CN207391440U - Cell co-culture device - Google Patents
Cell co-culture device Download PDFInfo
- Publication number
- CN207391440U CN207391440U CN201721138653.1U CN201721138653U CN207391440U CN 207391440 U CN207391440 U CN 207391440U CN 201721138653 U CN201721138653 U CN 201721138653U CN 207391440 U CN207391440 U CN 207391440U
- Authority
- CN
- China
- Prior art keywords
- culture
- hole
- cell
- wall
- buffer area
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides cell co-culture device, including at least three culture holes, culture plate, upper cover, culture hole is arranged in matrix form on culture plate, it is tangent two-by-two to cultivate outer wall of hole, at least three adjacent culture outer wall of hole cross a buffer area, and through hole is offered in three at least two culture outer wall of hole cultivated in outer wall of hole of one buffer area of composition.Culture medium in the utility model culture hole can enter buffer area by through hole, and then it exchanges and exchanges between other cell culture mediums, make cell be not required to contact directly can reach culture medium exchange, the presence of buffer area ensures that culture hole inner cell will not intersect, ensure that the purity of cell, continue to cultivate available for later passages or carry out albumen and gene level detection.
Description
Technical field
The utility model is related to medical research equipment and laboratory equipments, and in particular to a kind of cell co-culture device.
Background technology
Basic medical research much needs to use the co-cultivation of two kinds of cells.Presently commercially available co-cultivation cell is expensive because of it
Price, it is impossible to meet the requirement that co-cultures for a long time.And it is divided into upper chamber and lower room, accommodates two kinds of different cells respectively, on
Lower confluent monolayer cells are separated by with polycarbonate membrane.It needs first to take out the upper chamber for having culture medium when observing lower room under the microscope.With with
Lower shortcoming：1. expensive 2. subsequent experimental implementation complexity 3. constantly moves upper chamber, cell contamination is easily caused.
Such as a kind of training for being used to cultivate cell of Patent No. CN201720021670.0 (publication number CN206375920U)
Ware is supported, is separated culture hole so as to fulfill co-cultivation as partition plate using filter membrane, but this method cost of manufacture is higher, and mistake
Filter membrane is easily broken the pollution for causing cell.
The content of the invention
In view of the problems of the existing technology, the utility model provides cell co-culture device, to solve above-mentioned at least one
Kind technical problem.
The technical solution of the utility model is：Cell co-culture device, including at least three culture holes, culture plate, upper cover,
The culture hole is arranged in matrix form on the culture plate, and the culture outer wall of hole is tangent two-by-two, and at least three is adjacent
Culture outer wall of hole crosses a buffer area, three at least two culture holes cultivated in outer wall of hole of one buffer area of composition
Through hole is offered on outer wall,
The aperture of the through hole is between 1.5mm-3mm.The aperture of the through hole is preferably 2mm.
Bottom 4mm-4.5mm of the through hole apart from the culture hole.Preferably 4.28mm.
Preferably, offer in the culture outer wall of hole of through hole, the number of openings opened up is three, and same culture outer wall of hole
On three through holes equidistantly arrange in the horizontal direction, the spacing of two neighboring through hole is in 0.5mm-1.2mm.
The floor space of the culture hole is in 4.67cm2±0.5mm2。
The quantity for surrounding the culture hole of buffer area is no more than four.
Preferably, the upper cover can be closely sealed with culture plate.
Preferably, the quantity of culture hole is three, four, six etc..
Advantageous effect：When the utility model is used, opening upper cover, being added in each culture hole needs to co-culture not
With species suspension cell, culture medium is supplied to 1.5ml, after its is adherent, supplies culture medium to 5ml, the training in each culture hole
Foster base can enter buffer area by through hole, and then exchange and exchange between other cell culture mediums, and cell is made to be not required to directly connect
It touches and can reach culture medium exchange, the presence of buffer area ensures that culture hole inner cell will not intersect, and ensure that the purity of cell,
Continue to cultivate available for later passages or carry out albumen and gene level detection.The utility model limits the position of through hole, energy
It enough prevents through hole from opening up improper from outside outflow, causes the pollution of cell and the waste of culture medium.
Description of the drawings
Fig. 1 is the structure diagram of the utility model；
Fig. 2 is the structure diagram of another embodiment of the utility model.
In figure：1 is culture hole, and 2 be culture plate, and 3 be buffer area, and 4 be through hole, and 5 be the first culture hole, and 6 be the second training
Hole is supported, 7 be the 3rd culture hole, and 8 be the 4th culture hole.
Specific embodiment
The utility model is described further below in conjunction with the accompanying drawings.
Embodiment 1
Cell co-culture device, including three culture holes 1, culture plate 2, upper cover, culture hole is arranged in described in matrix form
On culture plate, the culture outer wall of hole is tangent two-by-two, and three adjacent culture outer wall of hole cross a buffer area 3, composition
There are two through hole 4 is offered in culture outer wall of hole in three culture outer wall of hole of one buffer area, the aperture of through hole is in 2mm.
It is used to implement the co-cultivation of two kinds of cells.
Embodiment 2
As shown in Fig. 2, cell co-culture device, there are four culture hole settings, is respectively the culture of the first culture hole 5, second
Hole 6, the 3rd culture hole 7 and the 4th culture hole 8, the first culture hole, the second culture hole, the 3rd culture hole and the 4th culture hole are in square
Configuration is arranged, and tangent crosses buffer area 3 mutually；Four curved walls of buffer area 3 are surrounded, every side wall is one
Cultivate a quarter of outer wall of hole.
First culture hole, the second culture hole, the 3rd culture hole and the 4th culture hole outer wall on offer through hole.For reality
The co-cultivation of existing four kinds of cells.
Embodiment 3
As shown in Figure 1, cell co-culture device, there are six culture hole settings, is respectively the first culture hole, the second culture
Hole, the 3rd culture hole, the 4th culture hole, the 5th culture hole and the 6th culture hole, the first culture hole, the second culture hole, the 3rd training
Support hole, the 4th culture hole, the 5th culture hole and the 6th culture hole are arranged in matrix form, and mutual tangent cross two buffering areas
Domain；
First culture hole, the second culture hole, the 3rd culture hole are in same horizontal line, the 4th culture hole, the 5th culture hole,
6th culture hole same horizontal line；
First culture hole, the second culture hole, the 4th culture hole and the 5th culture hole cross first buffering area domain, the second culture
Hole, the 3rd culture hole, the 5th culture hole and the 6th culture hole cross second buffering area domain；
Through hole or the second culture are offered in first culture hole, the second culture hole, the 5th culture hole and the 6th culture hole
Through hole is offered in hole, the 4th culture hole, the 3rd culture hole and the 5th culture hole.It can be carried out at the same time what two groups of cells co-cultured
Experiment.
It, can be directly using commercially available six orifice plate as culture plate there are six culture hole is set in the present embodiment.It saves into
This, convenient for experimental implementation.
It the above is only the preferred embodiment of the present invention, it is noted that come for those skilled in the art
It says, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (6)
1. cell co-culture device, which is characterized in that including at least three culture holes, culture plate, upper cover, the culture hole is in square
Configuration is arranged on the culture plate, and the culture outer wall of hole is tangent two-by-two, and at least three adjacent culture outer wall of hole cross
One buffer area is offered at least two culture outer wall of hole in three of one buffer area culture outer wall of hole of composition logical
Hole,
The aperture of the through hole is in 1.5mm-3mm.
2. cell co-culture device according to claim 1, it is characterised in that：The through hole is apart from the bottom of the culture hole
Portion 4mm-4.5mm.
3. cell co-culture device according to claim 1, it is characterised in that：It offers in the culture outer wall of hole of through hole,
The number of openings opened up is three, and three through holes in same culture outer wall of hole are equidistantly arranged in the horizontal direction, adjacent two
The spacing of a through hole is in 0.5mm-1.2mm.
4. cell co-culture device according to claim 1, it is characterised in that：The floor space of the culture hole is in 4.67cm2
±0.5mm2。
5. cell co-culture device according to claim 1, it is characterised in that：The upper cover can be closely sealed with culture plate.
6. cell co-culture device according to claim 1, it is characterised in that：The quantity of culture hole for three, four, six
It is a.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721138653.1U CN207391440U (en) | 2017-09-06 | 2017-09-06 | Cell co-culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721138653.1U CN207391440U (en) | 2017-09-06 | 2017-09-06 | Cell co-culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207391440U true CN207391440U (en) | 2018-05-22 |
Family
ID=62415830
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721138653.1U Expired - Fee Related CN207391440U (en) | 2017-09-06 | 2017-09-06 | Cell co-culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207391440U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109666587A (en) * | 2018-10-24 | 2019-04-23 | 海南医学院 | A kind of dynamic cell culture plate |
-
2017
- 2017-09-06 CN CN201721138653.1U patent/CN207391440U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109666587A (en) * | 2018-10-24 | 2019-04-23 | 海南医学院 | A kind of dynamic cell culture plate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN202621208U (en) | Novel test tube rack | |
| CN201695031U (en) | Partitioned culture vessel with glass bottom | |
| KR100718158B1 (en) | Cell co-culture device | |
| US20160002585A1 (en) | Cell culture dish supporting simultaneously juxtaposed and separated cultures | |
| CN207391440U (en) | Cell co-culture device | |
| CN105695328A (en) | Co-culture device for cell culture and cell-cell interaction and using method of co-culture device | |
| Jo et al. | Microfluidic Platforms for Yeast‐Based Aging Studies | |
| KR20200056331A (en) | Cell culture device | |
| CN102703318A (en) | Multi-chamber co-culture device | |
| CN208151368U (en) | A kind of biological culture dish | |
| CN207895144U (en) | For microscopical load sample device | |
| CN206328389U (en) | A kind of use for laboratory microbial culture dish for being easy to data record | |
| CN108504574A (en) | Culture bottle | |
| CN205473829U (en) | Interact's culture apparatus between cell culture and cell | |
| CN105670914A (en) | Petri dish capable of continuously supplementing impact factors | |
| CN204111769U (en) | A kind of many cells Dual culture and migration observing device | |
| CN211471437U (en) | Culture assembly | |
| CN203429184U (en) | Multi-adherent-cell coculture device | |
| CN207175974U (en) | A kind of multi-functional cell cultivation equipment | |
| CN209113928U (en) | Multilayer culture and antipollution attached cell culture apparatus | |
| CN201162013Y (en) | Large-scale sifting motion cultivation plate | |
| CN205856468U (en) | Tissue Culture Dish | |
| CN205803500U (en) | Tissue Culture Plate | |
| CN206375920U (en) | A kind of culture dish for being used to cultivate cell | |
| CN207891327U (en) | A kind of many cells co-cultivation ware frame |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180522 Termination date: 20180906 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |