CN207287738U - Multifunctional magnetic-force frame - Google Patents
Multifunctional magnetic-force frame Download PDFInfo
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- CN207287738U CN207287738U CN201721041515.1U CN201721041515U CN207287738U CN 207287738 U CN207287738 U CN 207287738U CN 201721041515 U CN201721041515 U CN 201721041515U CN 207287738 U CN207287738 U CN 207287738U
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- magnetic
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- force frame
- multifunctional
- outer shroud
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Abstract
The present utility model belongs to technical field of nucleic acid purification, and in particular to multifunctional magnetic-force frame, it includes at least the overall magnetic force portion in circular ring shape, and magnetic force portion includes inner ring, middle ring and the outer shroud stacked gradually from inside to outside；Middle ring includes at least magnetic ring, and the inner wall of inner ring is regularly evenly equipped with some interior fixed anchor rings；The outer wall of outer shroud is regularly evenly equipped with some outer fixed anchor rings.The utility model efficiency of magnetic separation is higher；Easily the multifunctional magnetic-force frame in the utility model can be installed on the common compact centrifuge in market and carry out centrifugally operated；Desired position is installed to again without PCR pipe or centrifuge tube are removed one by one, it is more convenient；A variety of test tubes can be adapted to.
Description
Technical field
The present utility model belongs to technical field of nucleic acid purification, and in particular to multifunctional magnetic-force frame, the magnetic frame are applicable in
Purify and elute in nucleic acid extraction, while the centrifugally operated in experimentation can be carried out.
Background technology
Paramagnetic particle method extraction nucleic acid is by lysate lysed sample, is specifically adsorbed from nucleic acid molecules after sample cracking
To magnetic-particle surface, and the impurity such as protein is not adsorbed and stayed in the solution.React certain time and then make in magnetic field
Magnetic-particle is separated with liquid under, recycling particle (i.e. magnetic bead-nucleic conjugate), then with cleaning solution wash removing it is non-specific
Property impurity of the absorption on magnetic bead, pure nucleic acid can be obtained by then carrying out elution.Paramagnetic particle method need not be centrifuged, is not required
Plurality of reagents is added, it is easy to operate, meet nucleic acid automation extraction and require, be that one of following nucleic acid purification method development is important
Direction.
Nucleic acid is separated and purified using Beads enrichment method, operating process is：Sample dissociation → magnetic bead is with separating core
Acid specific binding, washing → magnetic bead of magnetic separation → magnetic bead ＆ nucleic acid A conjugates are separated with the elution of nucleic acid to be separated and (cracked
→ combine, magnetic separation → washing → elution).After the completion of nucleic acid is separated and purified, it usually needs sample is carried out in next step
Processing, such as centrifugation, concussion etc. processing.
After sample cracking, magnetic bead is added into lysate, controls the acid-base value of solution, magnetic bead optionally with nucleic acid knot
Close, the magnetic bead for being adsorbed with nucleic acid is placed in magnetic field carries out magnetic separation at this time, it is possible to which the collection magnetic bead of orientation, then discards useless
Liquid, removes the non-specific impurity adsorbed (protein, fat, carbohydrate etc.) by rinsing liquid, then combines magnetic bead ＆ nucleic acid
Thing is placed in the eluent of high ph-values, and the nucleic acid of purifying can enter in eluent.Magnetic separation is required to produce the magnetic force in magnetic field
Device, the magnetic means are usually magnetic frame.
Current magnetic frame has the following disadvantages：
(1) existing magnetic frame is mostly to be developed, use scope for clinically common a certain consumptive material and special purpose
Narrow, user experience is not ideal enough.
(2) existing magnetic frame is mostly the magnetic-adsorption mode of fixed magnetic force and fixed position, in the effect of absorption and follow-up
There is many insufficient places in processing.
(3), it is necessary to remove centrifuge tube operation when existing magnetic frame earthquake and centrifugation, process is relatively complicated, takes.
Utility model content
To solve the above problems, the purpose of this utility model is to provide multifunctional magnetic-force frame, possesses magnetic separation function, at the same time
Also with regard to possessing fixed function.
The detailed technology scheme that the utility model is taken is：Multifunctional magnetic-force frame, it is in circular ring shape that it, which is included at least overall,
Magnetic force portion, magnetic force portion include inner ring, middle ring and the outer shroud stacked gradually from inside to outside；Middle ring includes at least magnetic ring, inner ring
Inner wall be regularly evenly equipped with some interior fixed anchor rings；The outer wall of outer shroud is regularly evenly equipped with some outer fixed anchor rings.
Preferably, the outer wall of outer shroud is obliquely sets, and the outer wall of outer shroud and the angle of horizontal plane are 90-85 °, can be with
Ensure the effect of centrifugation.
Further, middle ring further includes two and adjusts ladder；The terraced upper surface for being respectively fixedly disposed at magnetic ring of two adjustings,
The lower face of magnetic ring, adjusts ladder and is provided with adjusting mouth, and magnetic force portion further includes connection strap, the both ends of connection strap respectively with inner ring,
Outer shroud is fixedly connected, and connection strap face adjusts mouth.Required position is installed to again without PCR pipe or centrifuge tube are removed one by one
Put, it is more convenient.
Further, fixed part is further included；Fixed part includes stent and mounting hole, the axle center of mounting hole and the axis of inner ring
The heart overlaps, and the both ends of stent are fixedly connected with inner ring, mounting hole respectively.Can be easily by the multi-function magnetic in the utility model
Power frame is installed on the common compact centrifuge in market and carries out centrifugally operated.
Preferably, interior fixed anchor ring is made of elastic material.It can be automatically regulated according to the tube section of PCR pipe, and
Fixed effect is preferable.
Preferably, outer fixed anchor ring is made of hard elastic material, and outer fixed anchor ring is provided with partition.It can be adapted to
1.5mL or 2.0mL centrifuge tubes.
Following experimental data is the experiment carried out using the magnetic frame of the utility model.
1st, test sample type；It is serum sample respectively, buccal swab sample.
2nd, paramagnetic particle method extracts kit；Hepatitis B paramagnetic particle method extracts kit, brothers mouthful paramagnetic particle method extracts kit.
3rd, magnetic frame；A centrifuge tube purifying magnetic frame (control group) and the utility model magnetic frame on the market.
4th, experimental implementation；In experimentation, with an example sample, it is divided into two parts, two magnetic bead frame is respectively adopted and carries out magnetic bead
Adsorption and purification nucleic acid (i.e. magnetic separation).
5th, operate the computer；Real-time fluorescence quantitative PCR is carried out together, its CT value is compared and analyzed.
Data statistics result is as follows：
The CT values that can be seen that the utility model magnetic frame by above-mentioned data are located further forward, and illustrate that efficiency of magnetic separation is higher, i.e.,
Lost for the nucleic acid during reduction extraction purification, improve sensitivity and increase.
Compared with prior art, the utility model has the advantages that：1st, efficiency of magnetic separation is higher；2nd, can be easily
Multifunctional magnetic-force frame in the utility model is installed on the common compact centrifuge in market and carries out centrifugally operated；3rd, without inciting somebody to action
PCR pipe or centrifuge tube are removed one by one is installed to desired position again, more convenient；4th, a variety of test tubes can be adapted to.
Brief description of the drawings
Fig. 1 is the structure diagram of the multifunctional magnetic-force frame in the utility model.
Fig. 2 is the schematic diagram of the A-A sections of Fig. 1.
Magnetic force portion 1；Inner ring 11；Interior fixed anchor ring 111；Outer shroud 12；Outer fixed anchor ring 121；Middle ring 13；Magnetic ring 131；Adjust
Section ladder 132；Adjust mouth 1321；Connection strap 14；Fixed part 2；Stent 21；Mounting hole 22.
Embodiment
Please refer to Fig.1, Fig. 2, the utility model is multifunctional magnetic-force frame, it includes at least the overall magnetic force portion in circular ring shape
1。
Magnetic force portion 1 includes inner ring 11, middle ring 13 and the outer shroud 12 stacked gradually from inside to outside；Middle ring 13 includes at least magnetic
Power ring 131.Magnetic ring 131 can be the magnetic ring of the magnetic force 3500Gs of Suzhou ConRem Biomedical Technology Co., Ltd.；Certainly
Can also be other producers, the magnetic ring of specification.
The inner wall of inner ring 11 is regularly evenly equipped with some interior fixed anchor rings 111；If the outer wall of outer shroud 12 is regularly evenly equipped with
Dry outer fixed anchor ring 121.
Interior fixed anchor ring 111 is made of elastic material, for example medical grade rubber or medical silica-gel etc. are made.Interior retainer ring
Commonly used in adaptation PCR pipe etc., interior fixed anchor ring 111 made of elastic material can be automatic according to the tube section of PCR pipe in face 111
Ground is adjusted, and fixed effect is preferable.In general, interior fixed anchor ring 111 is adapted to the PCR pipe or eight connecting legs of 0.2mL.
Outer fixed anchor ring 121 is made (such as PP plastic stools) for hard elastic material, and outer fixed anchor ring 121 be provided with every
Disconnected (outer fixed anchor ring 121 can be divided into by two parts by partition, attached drawing is not drawn into).Outer fixed anchor ring 121 is usually used
In adaptation centrifuge tube.Outer fixed anchor ring 121 can be opened slightly, so as to be adapted to 1.5mL or 2.0mL centrifuge tubes.
The outer wall of outer shroud 12 is obliquely sets, and the outer wall of outer shroud 12 and the angle of horizontal plane are 90-85 °.It can protect
Demonstrate,prove the effect of centrifugation.
The operation principle of multifunctional magnetic-force frame in the utility model is：In use, by the examination equipped with the sample after cracking
Pipe fixes anchor ring 111 or outer fixed anchor ring 121 in loading, and sample is under the action of magnetic ring 131 produces magnetic force by magnetic
Choosing.
Middle ring 13 further includes two and adjusts ladder 132；Two adjust the upper end that ladder 132 is respectively fixedly disposed at magnetic ring 131
Face, the lower face of magnetic ring 131, adjust ladder 132 and are provided with adjusting mouth 1321, magnetic force portion 1 further includes connection strap 14, connection strap 14
Both ends be fixedly connected respectively with inner ring 11, outer shroud 12,14 face of connection strap adjust mouth 1321.Press two adjustings respectively with hand
Ladder 132, can move up and down magnetic ring 131 and fix (being fixed due to frictional force), so as to change magnetic as needed
The position of suction；When without magnetic separation (during than if desired for concussion), middle ring 13 need to only be moved up and only staggered with sample；Nothing
PCR pipe or centrifuge tube need to be removed one by one and be installed to desired position again, it is more convenient.
Multifunctional magnetic-force frame in the utility model further includes fixed part 2；Fixed part 2 includes stent 21 and mounting hole
22, the axle center of mounting hole 22 is overlapped with the axle center of inner ring 11, and the both ends of stent 21 are fixed with inner ring 11, mounting hole 22 respectively to be connected
Connect.The quantity of usual stent 21 is two or more, and each stent 21 is arranged in radioactivity.In use, by mounting hole
22 to it and are inserted into fixed pin with the axle center of centrifuge, easily can be installed on the multifunctional magnetic-force frame in the utility model
Centrifugally operated is carried out on the common compact centrifuge in market.
It is to be appreciated that term " " center ", " longitudinal direction ", " transverse direction ", "front", "rear", "left", "right", " vertical ", " water
It is flat ", " top ", " bottom ", " interior ", the orientation of the instruction such as " outer " or position relationship for based on orientation shown in the drawings or position relationship,
Only be for ease of the description present invention and simplify description, rather than instruction or infer the device of meaning or element must have it is specific
Orientation, be specific azimuth configuration and operation, thus it is not intended that protecting the limitation of content to the present invention.
Embodiment above is only the preferred embodiment of this creation, all in this wound not to limit this creation
Any modification, equivalent substitution, improvement and etc. done within the spirit and principle of work, should be included in this creation protection domain it
It is interior.
Claims (6)
1. multifunctional magnetic-force frame, it is characterised in that：Including at least it is overall be in circular ring shape magnetic force portion (1), magnetic force portion (1) include by
Inner ring (11), middle ring (13) and the outer shroud (12) stacked gradually from inside to outside；Middle ring (13) includes at least magnetic ring (131), interior
The inner wall of ring (11) is regularly evenly equipped with some interior fixed anchor rings (111)；The outer wall of outer shroud (12) is regularly evenly equipped with some outer
Fixed anchor ring (121).
2. multifunctional magnetic-force frame as claimed in claim 1, it is characterised in that：The outer wall of the outer shroud (12) is obliquely to set
Put, and the outer wall of outer shroud (12) and the angle of horizontal plane are 90-85 °.
3. multifunctional magnetic-force frame as claimed in claim 1, it is characterised in that：The middle ring (13) further includes two and adjusts ladder
(132)；Two adjust terraced (132) and are respectively fixedly disposed at the upper surface of magnetic ring (131), the lower face of magnetic ring (131), adjust
Section terraced (132), which is provided with, adjusts mouth (1321), and the magnetic force portion (1) further includes connection strap (14), the both ends point of connection strap (14)
It is not fixedly connected with inner ring (11), outer shroud (12), connection strap (14) face adjusts mouth (1321).
4. multifunctional magnetic-force frame as claimed in claim 3, it is characterised in that：Further include fixed part (2)；Fixed part (2) includes
Stent (21) and mounting hole (22), the axle center of mounting hole (22) are overlapped with the axle center of the inner ring (11), and the two of stent (21)
End is fixedly connected with inner ring (11), mounting hole (22) respectively.
5. the multifunctional magnetic-force frame as described in claim 1-4 is any, it is characterised in that：The interior fixed anchor ring (111) is bullet
Property material is made.
6. multifunctional magnetic-force frame as claimed in claim 5, it is characterised in that：The outer fixed anchor ring (121) is hard elastic
Material is made, and fixes anchor ring (121) outside and be provided with partition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721041515.1U CN207287738U (en) | 2017-08-20 | 2017-08-20 | Multifunctional magnetic-force frame |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721041515.1U CN207287738U (en) | 2017-08-20 | 2017-08-20 | Multifunctional magnetic-force frame |
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| Publication Number | Publication Date |
|---|---|
| CN207287738U true CN207287738U (en) | 2018-05-01 |
Family
ID=62444826
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721041515.1U Active CN207287738U (en) | 2017-08-20 | 2017-08-20 | Multifunctional magnetic-force frame |
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| CN (1) | CN207287738U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109248793A (en) * | 2018-09-27 | 2019-01-22 | 中国辐射防护研究院 | A kind of separation of solid and liquid half built-in magnet test tube bracket system and its operating method |
| CN109332008A (en) * | 2018-11-06 | 2019-02-15 | 钱瑜 | A kind of blood testing centrifuge that test tube is fixed in convenience |
-
2017
- 2017-08-20 CN CN201721041515.1U patent/CN207287738U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109248793A (en) * | 2018-09-27 | 2019-01-22 | 中国辐射防护研究院 | A kind of separation of solid and liquid half built-in magnet test tube bracket system and its operating method |
| CN109332008A (en) * | 2018-11-06 | 2019-02-15 | 钱瑜 | A kind of blood testing centrifuge that test tube is fixed in convenience |
| CN109332008B (en) * | 2018-11-06 | 2020-12-01 | 岳欣 | Blood that conveniently fixes test tube detects uses centrifuge |
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