CN207252666U - Cell gradient cooling cryopreservation box - Google Patents
Cell gradient cooling cryopreservation box Download PDFInfo
- Publication number
- CN207252666U CN207252666U CN201721270902.2U CN201721270902U CN207252666U CN 207252666 U CN207252666 U CN 207252666U CN 201721270902 U CN201721270902 U CN 201721270902U CN 207252666 U CN207252666 U CN 207252666U
- Authority
- CN
- China
- Prior art keywords
- box
- box body
- liquid
- feeding tube
- stent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000005138 cryopreservation Methods 0.000 title claims description 6
- 238000001816 cooling Methods 0.000 title abstract description 10
- 238000007710 freezing Methods 0.000 claims description 29
- 230000008014 freezing Effects 0.000 claims description 29
- 239000002904 solvent Substances 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 2
- 239000003960 organic solvent Substances 0.000 abstract description 8
- 239000007788 liquid Substances 0.000 abstract description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Medical Preparation Storing Or Oral Administration Devices (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The embodiment of the utility model discloses a cell gradient cooling cryopreserving box, including box body and the support of fixing on the box body, form the cavity between support and the box body, organic solvent is stored in the cavity, the box cover is installed to the box body upper end, and the liquid feeding pipe that communicates with the cavity is installed to the box body outer wall, the top of liquid feeding pipe is located the upper end at box body top, the utility model discloses fix the support on the box body, when unscrewing the box cover, organic solvent can not spill; the top of the liquid feeding pipe is higher than the box body, so that the organic solution can not leak out when the screw cap is unscrewed.
Description
Technical field
Biological medicine insulation instrument field is the utility model is related to, is specifically a kind of cell gradient cooling freezing storing box.
Background technology
The use of existing cell gradient freezing storing box, before freeze-stored cell, first takes out the pipe holder that freezes in box, past
Organic solvent is added in box, then stent is put back in box, then whole box precooling, when freezing, the cryopreservation tube equipped with cell
It is put into freezing storing box, lid is twisted, and progressively cools down, and -20 DEG C are dropped to from 4 DEG C, then drops to -80 DEG C, then takes out freezing storing box, opens
Lid, cell taking-up is put into liquid nitrogen and is preserved.
Existing cell gradient freezing storing box has the disadvantages that：After stent is put back in box, when to twist the lid off again,
The organic solvent being equipped with box is easily spilt, and is especially taken out the cell being stored in -80 DEG C of freezing storing box and is moved to liquid
When in nitrogen tank, the lid of freezing storing box causes to be difficult to turn on, firmly if unscrewed lid, is easier to due to being placed in sub-zero temperature
The organic solvent for causing to be equipped with box leaks out.
Utility model content
In view of this, the utility model embodiment provides a kind of cell gradient cooling freezing storing box, and preventing from twisting the lid off takes
Organic solvent leaks out during cell cryopreservation tube.
The utility model embodiment discloses a kind of cell gradient cooling freezing storing box, including box body and the branch that is fixed on box body
Frame, forms cavity between the stent and box body, the cavity memory is placed with solvent, and the cassette upper end installs box lid, box
External wall is provided with the liquid-feeding tube connected with cavity, and the top of the liquid-feeding tube is located at the upper end at the top of box body.
Further, the liquid-feeding tube is obliquely installed.
Further, the stent is step type, and the stent is made of 2 cylinders, and the box body is cylinder, institute
The upper end for stating stent is connected with inboard wall of cartridge, and the radial direction of the stent along cylinder is uniformly provided with multiple freezing tube seats.
Further, the depth of the freezing tube seat and the cryopreservation tube stored are highly identical.
Further, screw capping is installed at the top of the liquid-feeding tube.
Further, the upper end of the screw capping is parallel with horizontal plane.
Further, the screw capping is cylinder, and the screw capping is internally provided with internal thread, and the liquid-feeding tube upper end is equipped with outer
Screw thread, the screw capping are threadedly coupled with liquid-feeding tube.
Cell gradient cooling freezing storing box, secures the stand on box body, stent is nothing disclosed in the utility model embodiment
What method was taken out.When so turning on box lid, organic solvent will not leak out, and the liquid-feeding tube connected with cavity is provided with box body outer wall,
Liquid-feeding tube is used for adding organic solution, and usually screw capping and liquid-feeding tube are connection status, only when the organic solution in box body not
Just need to turn on (being typically all before freeze-stored cell, add at room temperature) when enough, along with the overhead height of liquid-feeding tube is slightly higher
In box body, it ensure that organic solution will not leak out when turning on screw capping.
Brief description of the drawings
Fig. 1 is the utility model embodiment cell gradient cooling freezing storing box front view.
Fig. 2 is the utility model embodiment cell gradient cooling freezing storing box top view.
Embodiment
The following is a combination of the drawings in the embodiments of the present utility model, and the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describe, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole realities
Apply example.Based on the embodiment in the utility model, those of ordinary skill in the art institute without creative efforts
The every other embodiment obtained, shall fall within the protection scope of the present invention.
As depicted in figs. 1 and 2, the utility model embodiment discloses a kind of cell gradient cooling freezing storing box, including 1 He of box body
The stent 2 being fixed on box body, forms cavity 3 between the stent 2 and box body 1,3 memory of cavity is placed with solvent, institute
1 upper end of box body installation box lid 4 is stated, 1 outer wall of box body is provided with the liquid-feeding tube 5 connected with cavity, and the top of the liquid-feeding tube 5 is located at
The upper end at the top of box body 1.
In this embodiment, since stent is integrally fixed on box body, stent can not take out.When so turning on box lid,
Organic solvent will not leak out, and the liquid-feeding tube that is connected with cavity is being provided with box body outer wall, and liquid-feeding tube is used for adding organic solution,
And usually screw capping and liquid-feeding tube are connection status, just need to turn on (typically only when the organic solution in box body is inadequate
Before freeze-stored cell, add at room temperature), along with the overhead height of liquid-feeding tube is slightly above box body, ensure that when turning on screw capping
Organic solution will not leak out.
In one embodiment of the utility model, the liquid-feeding tube 5 is obliquely installed.The space for being not take up stent is obliquely installed,
And it is also convenient for liquid feeding.
In one embodiment of the utility model, the stent is step type, and the stent is made of 2 cylinders, described
Box body is cylinder, and the upper end of the stent is connected with inboard wall of cartridge, and the stent is uniformly provided with along the radial direction of cylinder
Multiple freezing tube seats 21.Freezing tube seat is used to place cryovial.
In one embodiment of the utility model, the depth of the freezing tube seat 21 and the cryopreservation tube stored are highly identical.
In one embodiment of the utility model, screw capping 6 is installed at the top of the liquid-feeding tube 5.Screw capping usually with liquid-feeding tube
For connection status, prevent organic solution from being leaked out from liquid-feeding tube.
In one embodiment of the utility model, the upper end of the screw capping 6 is parallel with horizontal plane.
In one embodiment of the utility model, the screw capping 6 is cylinder, and the screw capping 6 is internally provided with internal thread, described
Liquid-feeding tube upper end is equipped with external screw thread, and the screw capping 6 is threadedly coupled with liquid-feeding tube 5.It is threadedly coupled fastening and easily opens.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
Spiritual and any modification within principle, made, equivalent substitution of utility model etc., should be included in the protection of the utility model
Within the scope of.
Claims (7)
- The freezing storing box 1. cell gradient cools down, it is characterised in that including box body and the stent being fixed on box body, the stent and box Cavity is formed between body, the cavity memory is placed with solvent, and the cassette upper end installs box lid, and box side wall is provided with and sky The liquid-feeding tube of chamber connection, the top of the liquid-feeding tube is located at the upper end at the top of box body.
- The freezing storing box 2. cell gradient as claimed in claim 1 cools down, it is characterised in that the liquid-feeding tube is obliquely installed.
- The freezing storing box 3. cell gradient as claimed in claim 1 cools down, it is characterised in that the stent is stepped, the branch Frame is made of 2 cylinders, and the box body is cylinder, and the upper end of the stent is connected with inboard wall of cartridge, stent edge circle The radial direction of cylinder is uniformly provided with multiple freezing tube seats.
- The freezing storing box 4. cell gradient as claimed in claim 3 cools down, it is characterised in that the depth of the freezing tube seat and storage Cryopreservation tube it is highly identical.
- The freezing storing box 5. cell gradient as claimed in claim 1 cools down, it is characterised in that installation is twisted at the top of the liquid-feeding tube Lid.
- The freezing storing box 6. cell gradient as claimed in claim 5 cools down, it is characterised in that put down with horizontal plane the upper end of the screw capping OK.
- The freezing storing box 7. cell gradient as claimed in claim 6 cools down, it is characterised in that the screw capping is cylinder, described to twist Lid is internally provided with internal thread, and the liquid-feeding tube upper end is equipped with external screw thread, and the screw capping is threadedly coupled with liquid-feeding tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721270902.2U CN207252666U (en) | 2017-09-29 | 2017-09-29 | Cell gradient cooling cryopreservation box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721270902.2U CN207252666U (en) | 2017-09-29 | 2017-09-29 | Cell gradient cooling cryopreservation box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207252666U true CN207252666U (en) | 2018-04-20 |
Family
ID=61918398
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721270902.2U Active CN207252666U (en) | 2017-09-29 | 2017-09-29 | Cell gradient cooling cryopreservation box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207252666U (en) |
-
2017
- 2017-09-29 CN CN201721270902.2U patent/CN207252666U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant |