CN206876716U

CN206876716U - A kind of Western blot Allergic skin test kit

Info

Publication number: CN206876716U
Application number: CN201720768843.5U
Authority: CN
Inventors: 王永昌
Original assignee: SHAOXING MEIDIKANG BIOLOGICAL TECHNOLOGY Co Ltd
Current assignee: SHAOXING MEIDIKANG BIOLOGICAL TECHNOLOGY Co Ltd
Priority date: 2017-06-29
Filing date: 2017-06-29
Publication date: 2018-01-12
Anticipated expiration: 2027-06-29

Abstract

The utility model discloses a kind of Western blot Allergic skin test kit, belong to technical field of immunoassay, including the detection plate provided with reactive tank, the bottom of reactive tank is equipped with the test strips of allergen protein antigen coat, and 2~5mm gap is left between the both ends of test strips and the corresponding end of reactive tank；The height of reactive tank is 3~6mm；One end of detection plate is provided with the plug division for extending and being fixed suitable for machine, and the end of reactive tank forms a limited step higher than plug division.Test agent box is arranged to adapt to the structure of machine detection, lengthening broadening processing has been carried out to reactive tank, its height is 3~6mm, to prevent liquid from being overflowed from reactive tank.The test agent box being put into every time in machine can be multiple, and anaphylactogen is identified by machine, substantially increases operating efficiency.The gap at test strips both ends is used for liquid feeding and imbibition, leaves enough spaces and facilitates suction nozzle and feed head to place.Limited step facilitates fixture to position, and it is clamped kit and is put into detection machine.

Description

A kind of Western blot Allergic skin test kit

Technical field

Technical field of immunoassay is the utility model is related to, specifically, is related to a kind of Western blot Allergic skin test Kit.

Background technology

Western blotting is the method for certain albumen in the specific binding detection of complex sample according to antigen-antibody, can be to egg It is white to carry out quantitative and semi-quantitative analysis.In detection, by antigen samples immobilization on trace paper, the examination of Allergic skin test is formed Paper slip, the detection for allergenic specific IgE antibody in human serum.

Existing Allergic skin test kit can only detect a measurand, work every time by manually being detected Make less efficient.

Utility model content

The purpose of this utility model is to provide a kind of Machine automated Western blot Allergic skin test kit of adaptation.

To achieve these goals, this Western blot Allergic skin test kit includes the detection plate provided with reactive tank, The bottom of reactive tank is equipped with the test strips of allergen protein antigen coat, is stayed between the both ends of test strips and the corresponding end of reactive tank There is 2~5mm gap；The height of reactive tank is 3~6mm；One end of detection plate, which is provided with, to extend and is inserted suitable for what machine was fixed Socket part, the end of reactive tank form a limited step higher than plug division.

In above-mentioned technical proposal, test agent box is arranged to adapt to the structure of machine detection, due to the activity point of view of machine Greatly, lengthening broadening processing has been carried out to reactive tank, its height is 3~6mm, to prevent liquid from being overflowed from reactive tank.Put every time It can be multiple to enter the test agent box in machine, and anaphylactogen is identified by machine, substantially increases operating efficiency.Test strips The gap at both ends is used for liquid feeding and imbibition, and the width in gap is 2~5mm, leaves enough spaces and facilitates suction nozzle and feed head to put Put.Facilitate fixture to position by the limited step of end set one in reactive tank, it is clamped kit and be put into detection machine.

Specific scheme is provided with the limiting plate extended downwardly for the both sides of plug division, is to coordinate machine grafting between two limiting plates Slot.Preferably, the length that limiting plate extends downwardly flushes with the bottom land of reactive tank so that limiting plate can be used to balance and put When putting detection plate in the horizontal plane, the cooperation of reactive tank and machine can be facilitated again.

Another specific scheme is that reactive tank is dovetail groove.The opening of dovetail groove is easy to liquid feeding and imbibition, is easy to simultaneously Observation.

Another specific scheme is less than the detection plate width of reactive tank present position for the width of plug division.Facilitate fixture Gripping.

Another specific scheme is that one end both sides of the reaction tank bottom away from plug division are provided with balance support plate.So that detection Plate level is more steady when placing.

Another specific scheme is equipped with balance support plate for the side of reactive tank both ends bottom.Further such that detection plate It is more steady when horizontal positioned.

Brief description of the drawings

Fig. 1 is the overlooking the structure diagram of the utility model embodiment；

Fig. 2 is the main structure diagram of the utility model embodiment；

Fig. 3 is the present invention looks up structural representation of the utility model embodiment.

Wherein：1st, detection plate；2nd, reactive tank；3rd, test strips；4th, plug division；5th, limited step；6th, limiting plate；7th, slot； 8th, support plate is balanced.

Embodiment

With reference to embodiments and its accompanying drawing is further described in detail bright to the utility model, but the utility model and is not only limited In this.

Referring to Fig. 1 to Fig. 3, Western blot Allergic skin test kit includes detection plate 1, is arranged in detection plate 1 Reactive tank 2 and be placed on reactive tank 2 bottom test strips 3.Test strips 3 are by allergen protein antigen coat.

Wherein, reactive tank 2 is that reactive tank is dovetail groove, leave 2 between the both ends of test strips and the corresponding end of reactive tank~ 5mm gap, in the present embodiment, the gap at liquid feeding end is 2.5mm, and the gap at imbibition end is 3mm.The height of reactive tank be 3~ 6mm, the present embodiment 4mm.

Extend to form a plug division 4 at the liquid feeding end of detection plate 1, the width of plug division 4 is less than the present position of reactive tank 2 The width of detection plate 1, the plug division 4 are applicable to be fixed in detection machine.The end of reactive tank 2 is formed higher than plug division 4 One limited step 5, facilitates fixture to position, and it is clamped kit and is put into detection machine.It is provided with simultaneously in the both sides of plug division 4 The limiting plate 6 extended downwardly, to coordinate the slot 7 of machine grafting between two limiting plates 6, length that limiting plate 6 extends downwardly with it is anti- The bottom land of groove 2 is answered to flush.One end both sides of the bottom of reactive tank 2 away from plug division 4 are provided with balance support plate 8 so that the water of detection plate 1 It is more steady during placing flat.Also can be all provided with the side of reactive tank both ends bottom to balance support plate 8, for further increasing detection Stability when plate level is placed.

The detection process operating procedure of the present embodiment is as follows：

Step 1：All reagent components are balanced to room temperature (25 DEG C).Mark reactive tank after more can cleaning fluid moistening have nitre The reactive tank of sour tunica fibrosa, patient's 0.25ml serum is then separately added into reactive tank, is a person-portion per groove.

Step 2：It is incubated.Reactive tank is covered with film or the scraps of paper, then at room temperature on parallel oscillator with per minute The frequency oscillation of 30 times 45 minutes.

Step 3：Wash film.The serum in reactive tank is poured out, the wash bottle equipped with the cleaning fluid diluted is taken, is rinsed with cleaning fluid Individual test strips 5 seconds or 2 seconds * 3 times.Pour out the cleaning fluid in reactive tank.

Step 4：5 drop secondary antibody/biotins are added per groove.Repeat step 2：At room temperature with per minute on parallel oscillator The frequency oscillation of 30 times 45 minutes.

Step 5：Wash film.Repeat step 3.

Step 6：5 drop HRP enzyme streptavidins are added per groove.Repeat step 2：At room temperature with every point on parallel oscillator The frequency oscillation that clock is 30 times 45 minutes.

Step 7：Wash film.Repeat step 3.

Step 8：5 drop tmb substrates are added per groove.Reacted 10 ± 2 minutes with lucifuge at room temperature.

Step 9：With distilled water or deionized water rinsing test strips 5 seconds, the liquid in reactive tank is poured out.

Result judgement；Residual liquid in groove is blotted with clean blotting paper, is dried, band water mark corresponds to colorimetric card after all decorporating Visual observation is qualitative, or with Western blotting quantitative analysis instrument analysis result, and print examining report.

Preferable implementation example of the present utility model is the foregoing is only, is not limited to the utility model, it is all in this reality Within new spirit and principle, any modification, equivalent substitution and improvements made etc., guarantor of the present utility model should be included in Within the scope of shield.

Claims

1. a kind of Western blot Allergic skin test kit, including the detection plate provided with reactive tank, the bottom of the reactive tank It is equipped with the test strips of allergen protein antigen coat, it is characterised in that：

2~5mm gap is left between the both ends of the test strips and the corresponding end of reactive tank；

The height of the reactive tank is 3~6mm；

One end of the detection plate is provided with the plug division for extending and being fixed suitable for machine, and the end of the reactive tank is higher than described Plug division formed a limited step.

2. Western blot Allergic skin test kit as claimed in claim 1, it is characterised in that：The both sides of the plug division It is the slot for coordinating machine grafting between two limiting plates provided with the limiting plate extended downwardly.

3. Western blot Allergic skin test kit as claimed in claim 1, it is characterised in that：Described reactive tank is ladder Shape groove.

4. Western blot Allergic skin test kit as claimed in claim 1, it is characterised in that：The width of the plug division Less than the detection plate width of reactive tank present position.

5. Western blot Allergic skin test kit as claimed in claim 1, it is characterised in that：The reaction tank bottom is remote One end both sides from the plug division are provided with balance support plate.

6. Western blot Allergic skin test kit as claimed in claim 1, it is characterised in that：Reactive tank both ends bottom The side in portion is equipped with balance support plate.