CN206706116U - A kind of tissue digestion device - Google Patents
A kind of tissue digestion device Download PDFInfo
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- CN206706116U CN206706116U CN201720539209.4U CN201720539209U CN206706116U CN 206706116 U CN206706116 U CN 206706116U CN 201720539209 U CN201720539209 U CN 201720539209U CN 206706116 U CN206706116 U CN 206706116U
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- tissue digestion
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- digestion device
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Abstract
The utility model provides a kind of tissue digestion device, including：Magnetic agitation heater and the tissue digestion device being arranged on the magnetic agitation heater；Sample inlet, magnetic rotor entrance and filtrate inlet are provided with the tissue digestion device；Filter is provided with the filtrate inlet.Tissue digestion device of the present utility model is integral by magnetic agitation heater and tissue digestion device sets, convenient operation, and sample inlet, magnetic rotor entrance and the filtrate inlet of tissue digestion device of the present utility model are available for various tissue fluid to use, convenient sample-adding and filtering, avoid and transfer the pollution, it is simple to operate.
Description
Technical field
This practical biotechnology field, more particularly, to a kind of tissue digestion device.
Background technology
Before animal cell culture, animal tissue's block typically is handled with pancreatin, albumen (cell will be connected between its cell
Epimatrix) digest, tissue block is separated into individual cells, this process is exactly animal tissue's digestion.
Animal tissue's digestion has a variety of methods, such as enzyme digestion.And device is also varied.Prior art is disclosed to be filled
Put and mostly separately carried out for heater and slaking apparatus, and water bath heating device due to sterility requirements it is strict, it is cumbersome,
Intermediate transfer often easily causes pollution.
Utility model content
In view of this, the technical problems to be solved in the utility model is to provide a kind of tissue digestion device, and this practicality is new
The tissue digestion device that type provides is simple to operate, substantially reduces contamination probability.
The utility model provides a kind of tissue digestion device, including：
Magnetic agitation heater and the tissue digestion device being arranged on the magnetic agitation heater；
Sample inlet, magnetic rotor entrance and filtrate inlet are provided with the tissue digestion device；The filtrate inlet
On be provided with filter.
Preferably, the tissue digestion device is tissue digestion tank.
Preferably, a diameter of 11cm~13cm of tissue digestion tank；A diameter of 4cm of the sample inlet~
5cm；A diameter of 4cm~5cm of the magnetic rotor entrance；A diameter of 4cm~5cm of the filtrate inlet.
Preferably, the filter is filter screen；The filter screen is threadedly coupled with the filtrate inlet.
Preferably, the aperture of the filter screen is 7~10 μm.
Preferably, the first spiral cover is provided with the sample inlet；The second spiral cover is provided with the filtrate inlet.
Preferably, the 3rd spiral cover is provided with the magnetic rotor entrance, is set on the 3rd spiral cover fluted.
Preferably, the tissue digestion device includes magnetic rotor, and the magnetic rotor includes glass column and is arranged on institute
First bead and the second bead at glass column both ends are stated, and includes metal, the first bead in second bead
With the groove ball-joint of the 3rd spiral cover.
Preferably, magnetic force heating plate is provided with the magnetic agitation heater, the tissue digestion device is arranged at
In the magnetic force heating plate.
Preferably, speed control knob, temperature control knob, temperature display are provided with the magnetic agitation heater
Screen and rotating speed display screen；The temperature range of the temperature control knob is 0~40 DEG C.
Compared with prior art, the utility model provides a kind of tissue digestion device, including：Magnetic agitation heater
With the tissue digestion device being arranged on the magnetic agitation heater；Sample is provided with the tissue digestion device to enter
Mouth, magnetic rotor entrance and filtrate inlet；Filter is provided with the filtrate inlet.Tissue digestion dress of the present utility model
Magnetic agitation heater and tissue digestion device sets is integral, convenient operation is put, and tissue of the present utility model disappears
Disguising sample inlet, magnetic rotor entrance and the filtrate inlet put is available for various tissue fluid to use, and convenient sample-adding and filtering, avoids
Transfer the pollution, it is simple to operate.
Brief description of the drawings
Fig. 1 is the structure chart that Fig. 1 is the tissue digestion device described in the one of embodiment of the utility model；
Fig. 2 is the filtering that the tissue digestion device described in the one of embodiment of the utility model is arranged on filtrate inlet
Installation drawing.
Embodiment
The utility model provides the utility model and provides a kind of tissue digestion device, including：
Magnetic agitation heater and the tissue digestion device being arranged on the magnetic agitation heater；
Sample inlet, magnetic rotor entrance and filtrate inlet are provided with the tissue digestion device；The filtrate inlet
On be provided with filter.
Tissue digestion device provided by the utility model is available for various tissue fluid to digest, for known to those skilled in the art
Tissue fluid digestion, herein without limit.
Tissue digestion device provided by the utility model includes magnetic agitation heater.
Magnetic force heating plate is provided with magnetic agitation heater described in the utility model, the tissue digestion device is set
It is placed in the magnetic force heating plate.
It is of the present utility model tissue digestion device to be heated by the magnetic force heating plate and magnetic agitation, avoid making
With the heating water bath set of prior art, also avoid using existing temperature probe, it is easier.
The utility model is known without restriction, those skilled in the art for the specification and material of the magnetic force heating plate
.The magnetic force heating plate diameter or width is preferably 10~12cm；The material of the magnetic force heating plate is preferably TU2 purples
Copper coin vacuum coating/black brilliant ceramic panel.
The utility model for described magnetic agitation heater shape without limit, can be art technology
Cuboid known to personnel or square.The utility model for described magnetic agitation heater specification without limit
It is fixed, tissue digestion device can be carried.
The utility model is for being provided with speed control knob on described magnetic agitation heater, temperature control is revolved
Button, temperature display and rotating speed display screen；Preferably, rotating speed control rotation is provided with a side of magnetic agitation heater
Button, temperature control knob, temperature display and rotating speed display screen.
The utility model is for the speed control knob, temperature control knob, temperature display and rotating speed display screen
Specific material and control system are well known to those skilled in the art without limiting.
Wherein, the temperature range of the temperature control knob is preferably 0~40 DEG C；More preferably 20~40 DEG C；Rotating speed control
The range of speeds of knob processed is preferably 0~300r/min；The utility model is for magnetic agitation heater said temperature and turns
The setting of speed, the requirement of the actual temp and rotating speed of tissue digestion is more bonded, temperature control is more sensitive and accurate, and digestion is more thorough
Bottom.
The utility model for the magnetic agitation heater source without limit, can be it is commercially available, can also
Made by oneself according to specification described in the utility model and demand.
The tissue that tissue digestion device provided by the utility model includes being arranged on the magnetic agitation heater disappears
Makeup is put.
The utility model, without limiting, preferably can be tissue digestion tank for the tissue digestion device；More preferably
For three mouthfuls of tissue digestion tanks.
Wherein, tissue digestion tank includes tank body and three entrances, specifically：
Sample inlet, magnetic rotor entrance and filtrate inlet are provided with the tissue digestion device；The filtrate inlet
On be provided with filter.
Tissue digestion tank diameter described in the utility model is preferably 11cm~13cm；More preferably 11cm~12cm；
The highly preferred of the tank body is 15~17cm, more preferably 15~16cm.
The utility model for tissue digestion tank material without limit, it is well known to those skilled in the art.
Tissue digestion tank sample inlet described in the utility model is preferably circular, and the diameter of the sample inlet is preferably
4cm~5cm；The tissue digestion tank magnetic rotor entrance is preferably circular, and the diameter of the magnetic rotor entrance is preferably 4cm
~5cm；The tissue digestion tank filtrate inlet is preferably circular, and the diameter of the filtrate inlet is preferably 4cm~5cm.
Filter is provided with filtrate inlet described in the utility model.The filter is filter screen；The filter screen with
The filtrate inlet threaded connection.
The utility model for the filter screen material without limit, filtering tissue fluid well known to those skilled in the art
Filter screen.The aperture of the filter screen is preferably 7~10 μm；More preferably 7~9 μm；Most preferably 7~8 μm.
The first spiral cover is provided with sample inlet described in the utility model；First spiral cover and the sample inlet screw thread
Connection.The second spiral cover is provided with filtrate inlet described in the utility model；Second spiral cover connects with the filtrate inlet screw thread
Connect.
The 3rd spiral cover, the 3rd spiral cover and magnetic rotor entrance are provided with magnetic rotor entrance described in the utility model
Threaded connection.Set on 3rd spiral cover fluted.
The utility model for first spiral cover, the second spiral cover and the 3rd spiral cover material without limit, this area
It is preferably identical with the tissue digestion tank known to technical staff.
In the utility model, the tissue digestion device includes magnetic rotor.
Magnetic rotor described in the utility model include glass column and be arranged on the glass column both ends the first bead and
Second bead, and include metal in second bead, the groove ball-joint of the first bead and the 3rd spiral cover.
The metal is preferably stainless steel.The diameter of first bead described in the utility model is preferably 0.8~1.1cm；
More preferably 0.9~1cm；The diameter of second bead is preferably 0.8~1.1cm；More preferably 0.9~1cm；The glass
The height of glass post is preferably 14~16cm, more preferably 14~15cm.
The utility model by the setting of above three entrance, can cause tissue digestion for needing filtering, without mistake
The tissue fluid of filter and stirring can be used, and the scope of application is wider, the tissue fluid filtered especially for needs, directly pour into i.e.
Can, the step of avoiding conventional filtering and transfer, reduce pollution.
The setting of the specific magnetic rotor of the utility model simultaneously to be stirred more uniformly, and digestion is more thorough
Bottom.
The utility model provides a kind of tissue digestion device, including：Magnetic agitation heater and it is arranged at the magnetic
Tissue digestion device on power agitating heater；Sample inlet, magnetic rotor entrance are provided with the tissue digestion device
And filtrate inlet；Filter is provided with the filtrate inlet.Tissue digestion device of the present utility model adds magnetic agitation
Thermal and tissue digestion device sets are integral, convenient operation, and the sample of tissue digestion device of the present utility model enters
Mouth, magnetic rotor entrance and filtrate inlet are available for various tissue fluid to use, and convenient sample-adding and filtering, avoid and transfer the pollution, behaviour
Make simple.
Fig. 1 is the structure chart of the tissue digestion device described in the one of embodiment of the utility model, wherein:
1 is magnetic agitation heater, and 2 be magnetic force heating plate, and 3 be speed control knob, and 4 be rotating speed display screen, and 5 be temperature
Control handle is spent, 6 be temperature display, and 7 be the first spiral cover, and 8 be the 3rd spiral cover, and 9 be the second spiral cover, and 10 be magnetic rotor, 11
For sample inlet；12 be magnetic rotor entrance；13 be filtrate inlet.Filter 14 is additionally provided with described 13, such as Fig. 2 institutes
Show, Fig. 2 is the filter that the tissue digestion device described in the one of embodiment of the utility model is arranged on filtrate inlet
Figure.
In order to further illustrate the utility model, with reference to embodiments to tissue digestion device provided by the utility model
It is described in detail.
Embodiment 1
According to structure organization slaking apparatus described in the utility model.Specially：Length and width is 20cm, a height of 10cm's
Magnetic agitation heater, is provided with a diameter of 12cm of magnetic force heating plate on device, the scope of temperature control knob for 20~
40 DEG C, the range of speeds is 0~300r/min.Tissue digestion device is arranged in magnetic force heating plate, is three mouthfuls of tissue digestion tanks.Sample
Product entrance, magnetic rotor entrance and filtrate inlet diameter are 5cm, and are provided with spiral cover.Tissue digestion tank is highly 15cm,
Base diameter is 11cm.Filtrate inlet is provided with 7 μm of screen packs and spiral cover, a diameter of 1cm the first bead and the spiral cover
Groove ball-joint, be stainless steel metal ball in the bead of rotor second, a diameter of 1cm.
Embodiment 2
Tissue digestion device is installed according to the structure described in the utility model embodiment 1, it is sterile to be operated：Tissue
Block is cut into small pieces 1cm*1cm, is directly put into from sample inlet in tissue digestion tank, starter.
Embodiment 3
Tissue digestion device is installed according to the structure described in the utility model embodiment 1, it is sterile to be operated：Need to filter
Tissue fluid, be put into from filtrate inlet in tissue digestion tank, starter.
Described above is only preferred embodiment of the present utility model, it is noted that for the common skill of the art
For art personnel, on the premise of the utility model principle is not departed from, some improvements and modifications can also be made, these improve and
Retouching also should be regarded as the scope of protection of the utility model.
Claims (10)
- A kind of 1. tissue digestion device, it is characterised in that including：Magnetic agitation heater and the tissue digestion device being arranged on the magnetic agitation heater；Sample inlet, magnetic rotor entrance and filtrate inlet are provided with the tissue digestion device；Set on the filtrate inlet It is equipped with filter.
- 2. device according to claim 1, it is characterised in that the tissue digestion device is tissue digestion tank.
- 3. device according to claim 2, it is characterised in that a diameter of 11cm~13cm of tissue digestion tank； A diameter of 4cm~5cm of the sample inlet；A diameter of 4cm~5cm of the magnetic rotor entrance；The filtrate inlet A diameter of 4cm~5cm.
- 4. device according to claim 1, it is characterised in that the filter is filter screen；The filter screen and the filter Liquid inlet screw connects.
- 5. device according to claim 4, it is characterised in that the aperture of the filter screen is 7~10 μm.
- 6. according to the device described in claim 1, it is characterised in that be provided with the first spiral cover on the sample inlet；The filtrate The second spiral cover is provided with entrance.
- 7. device according to claim 6, it is characterised in that the 3rd spiral cover, institute are provided with the magnetic rotor entrance State set on the 3rd spiral cover it is fluted.
- 8. device according to claim 7, it is characterised in that the tissue digestion device includes magnetic rotor, the magnetic Power rotor includes glass column and is arranged on first bead and the second bead at the glass column both ends, and second glass Include metal, the groove ball-joint of the first bead and the 3rd spiral cover in pearl.
- 9. device according to claim 1, it is characterised in that magnetic force heating is provided with the magnetic agitation heater Plate, the tissue digestion device are arranged in the magnetic force heating plate.
- 10. device according to claim 9, it is characterised in that be provided with rotating speed control on the magnetic agitation heater Knob, temperature control knob, temperature display and rotating speed display screen processed；The temperature range of the temperature control knob is 0~40 ℃。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720539209.4U CN206706116U (en) | 2017-05-12 | 2017-05-12 | A kind of tissue digestion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720539209.4U CN206706116U (en) | 2017-05-12 | 2017-05-12 | A kind of tissue digestion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206706116U true CN206706116U (en) | 2017-12-05 |
Family
ID=60464049
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720539209.4U Active CN206706116U (en) | 2017-05-12 | 2017-05-12 | A kind of tissue digestion device |
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| Country | Link |
|---|---|
| CN (1) | CN206706116U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108753611A (en) * | 2018-06-06 | 2018-11-06 | 青岛农业大学 | The soft tissue slaking apparatus of primitive cell culture |
-
2017
- 2017-05-12 CN CN201720539209.4U patent/CN206706116U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108753611A (en) * | 2018-06-06 | 2018-11-06 | 青岛农业大学 | The soft tissue slaking apparatus of primitive cell culture |
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