CN206646095U - One-stop site nucleic acid augmentation detection carrying case - Google Patents
One-stop site nucleic acid augmentation detection carrying case Download PDFInfo
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- CN206646095U CN206646095U CN201720197375.0U CN201720197375U CN206646095U CN 206646095 U CN206646095 U CN 206646095U CN 201720197375 U CN201720197375 U CN 201720197375U CN 206646095 U CN206646095 U CN 206646095U
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- nucleic acid
- casing
- stop site
- carrying case
- augmentation detection
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Abstract
It the utility model is related to a kind of one-stop site nucleic acid augmentation detection carrying case, including casing and case lid, the case lid is covered on casing, pipettor is provided with the casing, electronic broken instrument, centrifuge, metal bath, isothermal duplication fluorescence detector and cell panel, the configuration of basic reaction solution can be completed using the one-stop site nucleic acid augmentation detection carrying case, sample crushes, boiling lysis, the experimental implementation such as the extraction of nucleic acid and upper machine augmentation detection, the flow of whole nucleic acid isothermal amplification detection is completed, simultaneously, using cell panel directly to the electronic broken instrument, centrifuge and metal bath power supply, extraneous it need not be powered in whole experiment process, so that nucleic acid isothermal amplification detection can be completed at the scene, do not restricted by environment.
Description
Technical field
The utility model belongs to cause of disease field detecting device technical field, and in particular to a kind of one-stop site nucleic acid amplification
Detect carrying case.
Background technology
In the prior art, the methods of conventional detection for cause of disease mainly uses cell culture, serological test, it is uncomfortable
Close early stage quick detection, it is difficult to adapt to the needs of epidemic situation control.Immune colloidal gold technique, Standard PCR and the DNA of developed recently are visited
Pin hybridization technique, still suffer from specificity and the problem of sensitiveness.Although Real-Time Fluorescent Quantitative PCR Technique high sensitivity, reliability
It is good, it is totally-enclosed reaction, but the technology needs expensive quantitative real time PCR Instrument, in basic health department and can not prevent
Epidemic disease mechanism popularizes, and to ensure that high specific generally requires fluorescence labeling probe, and testing cost is higher.It should be noted simultaneously
It is that accurate expensive instrument requires higher to the professional ability of quality control, operating environment and personnel, it is difficult in epidemic situation scene, open country
The places such as outside, occasion is ready to go out on duty use, the energy source and power (220V civil powers) needed for operational outfit detection scene also usually without
Method guarantee.
Compared with above-mentioned technological means, the amplified reaction overall process of nucleic acid isothermal amplification detection technique is at the same temperature
Carry out, be not necessary to need to undergo the cyclic process of tens temperature changes as PCR reactions, therefore want instrument needed for amplification
Ask and greatly simplify, the reaction time shortens, and has huge application value, is especially suitable for field quick detection.Wherein, ring mediation etc.
Temperature amplification (Loop-mediatedIsothermalAmplification, LAMP) technology, amplification efficiency and detection sensitivity can
Than high 10-100 times or so of regular-PCR, as a result differentiate easy, such as precipitation turbidity or metal ion chelation agent of detection reaction tube
Color change can differentiate yin and yang attribute, the requirement for sample treatment, operating technology and instrument and equipment is preferably all than relatively low
Used in the poor basic health in epidemic situation scene or condition and epidemic prevention organization.
But, it is necessary to the heat circulating equipment of load, the detection of amplified production when with nucleic acid isothermal amplification detection technique
Also it is more complicated, it is necessary to a set of complicated flow and equipment.Therefore this just governs nucleic acid isothermal amplification detection technique at the scene
Application.
Utility model content
In order to solve above mentioned problem existing for prior art, realize that nucleic acid isothermal amplification detection can be completed at the scene, this reality
A kind of one-stop site nucleic acid augmentation detection carrying case, the one-stop site nucleic acid augmentation detection carrying case are provided with new
According to the requirement of nucleic acid isothermal amplification detection technique, it is equipped with required for nucleic acid extraction can be completed under simple environment and is detected
Instrument and equipment, and would be integrated into a case being convenient for carrying, it is achieved thereby that showing nucleic acid isothermal amplification detection
Field application.
Technical scheme is used by the utility model：One-stop site nucleic acid augmentation detection carrying case, including casing and
Case lid, the case lid are covered on casing, and pipettor, electronic broken instrument, centrifuge, metal bath, isothermal are provided with the casing
Amplification fluorescent detector and cell panel, the cell panel respectively with electronic broken instrument, centrifuge, metal bath and the isothermal duplication
Fluorescence detector electrically connects；The box house is provided with multiple dividing plates, and casing is divided into space independent of each other by the dividing plate,
The pipettor, electronic broken instrument, centrifuge, metal bath, isothermal duplication fluorescence detector and cell panel are separately positioned on difference
In space.
Contain pipettor, electronic broken instrument, centrifuge, metal bath in the one-stop site nucleic acid augmentation detection carrying case
With isothermal duplication fluorescence detector, basic reaction solution can be completed using the one-stop site nucleic acid augmentation detection carrying case
The experimental implementation such as configuration, the broken of sample, the extraction of boiling lysis, nucleic acid and upper machine augmentation detection, completed whole nucleic acid
The flow of isothermal duplication detection, meanwhile, include cell panel in the one-stop site nucleic acid augmentation detection carrying case, using battery
Plate is directly powered to the electronic broken instrument, centrifuge and metal bath, extraneous need not be powered in whole experiment process so that
Nucleic acid isothermal amplification detects, and can complete, not restricted by environment at the scene.
As a kind of mode for connecting and opening, one end of the casing and case lid is hinged, and the other end is removable by buckle
Unload connection.
Carried in order to facilitate experimenter, preferable technical scheme is that the casing is provided with handle.
In order to protect the experiment equipment in the one-stop site nucleic acid augmentation detection carrying case, preferable technical scheme
It is that the surface of the dividing plate is provided with air bag, and gas vent is offered on the air bag.When in use, experiment equipment is put into difference
Space after, inflated by the gas vent into air bag, air bag plays buffering and fixed effect to experiment equipment after heaving,
When needing to take out experiment equipment, open gas vent and release the gas in air bag, you can be taken out conveniently experiment equipment.
As the mode of another convenient fixed experiment equipment, the interior shape in the space is with being arranged in the space
The pipettor in portion, electronic broken instrument, centrifuge, metal bath, the shape of isothermal duplication fluorescence detector or cell panel are engaged, institute
The surface for stating dividing plate is provided with liner.Wherein liner can play a part of bumper and absorbing shock.
Further, the liner is made of foamed material.
The beneficial effects of the utility model are：It is portable that the utility model provides a kind of one-stop site nucleic acid augmentation detection
Case, using the one-stop site nucleic acid augmentation detection carrying case can complete the configuration of basic reaction solution, sample it is broken,
The experimental implementations such as boiling lysis, the extraction of nucleic acid and upper machine augmentation detection, the flow of whole nucleic acid isothermal amplification detection is completed,
Meanwhile include cell panel in the one-stop site nucleic acid augmentation detection carrying case, using cell panel directly to described electronic broken
Broken instrument, centrifuge and metal bath power supply, extraneous need not power in whole experiment process so that nucleic acid isothermal amplification detection exists
Scene can complete, and not restricted by environment.
Brief description of the drawings
Fig. 1 is the structural representation of embodiment in the utility model；
Fig. 2 is embodiment middle case internal structure schematic diagram in the utility model.
In figure：1st, casing；11st, handle；2nd, dividing plate；3rd, pipettor；4th, electronic broken instrument；5th, centrifuge；6th, metal bath；7、
Isothermal duplication fluorescence detector；8th, cell panel；9th, air bag.
Embodiment
With reference to embodiment, content of the present utility model is further illustrated.It should be appreciated that implementation of the present utility model
The following examples are not limited to, any formal accommodation and/or change made to the utility model fall within this
Utility model protection scope.
As depicted in figs. 1 and 2, the utility model provides a kind of one-stop site nucleic acid augmentation detection carrying case, including
Casing 1 and case lid (not shown in Fig. 1), the casing 1 are provided with handle 11, and the case lid is covered on casing 1, the casing
1 and one end of case lid be hinged, the other end is detachably connected by buckle；Pipettor 3, electronic broken instrument are provided with the casing 1
4th, centrifuge 5, metal bath 6, isothermal duplication fluorescence detector 7 and cell panel 8, the cell panel 8 respectively with it is described electronic broken
Instrument 4, centrifuge 5, metal bath 6 and isothermal duplication fluorescence detector 7 electrically connect；The inside of casing 1 is provided with multiple dividing plates 2, institute
State dividing plate 2 and casing 1 be divided into space independent of each other, the pipettor 3, electronic broken instrument 4, centrifuge 5, metal bath 6, etc.
Warm amplification fluorescent detector 7 and cell panel 8 are separately positioned in different spaces.
Contain pipettor 3, electronic broken instrument 4, centrifuge 5, gold in the one-stop site nucleic acid augmentation detection carrying case
Category bath 6 and isothermal duplication fluorescence detector 7, can be completed basic using the one-stop site nucleic acid augmentation detection carrying case
The experimental implementations such as configuration, the broken of sample, the extraction of boiling lysis, nucleic acid and the upper machine augmentation detection of reaction solution, have been completed whole
The flow of individual nucleic acid isothermal amplification detection, meanwhile, include cell panel 8 in the one-stop site nucleic acid augmentation detection carrying case,
Directly powered using cell panel 8 to electronic broken instrument 4, centrifuge 5 and the metal bath 6, need not in whole experiment process
External world's power supply so that nucleic acid isothermal amplification detects, and can complete, not restricted by environment at the scene.
The surface of the dividing plate 2 is provided with air bag 9, and gas vent is offered on the air bag 9.When in use, by experiment equipment
After being put into different spaces, inflated by the gas vent into air bag 9, air bag 9 heave after to experiment equipment play buffering and
Fixed effect, when needing to take out experiment equipment, open gas vent and release the gas in air bag 9, you can be taken out conveniently
Experiment equipment.
Present inventor can cause the cumbersome of operation it is considered that inflation/deflation using air bag 9 repeatedly；And make
Once used time air bag 9 ruptures, the disappearance of buffer protection function can be directly resulted in, to solve the above problems, inventor devises separately
A kind of mode of fixed experiment equipment：The interior shape in the space and the pipettor 3, electronic broken for being arranged on the interior volume
Broken instrument 4, centrifuge 5, metal bath 6, the shape of isothermal duplication fluorescence detector 7 or cell panel 8 are engaged, the table of the dividing plate 2
Face is provided with and uses liner made of foamed material, and the liner can play a part of bumper and absorbing shock.
In the present embodiment, the isothermal duplication fluorescence detector is OptiGene Limited of Britain
GenieIII。
It should be noted last that above example is only unrestricted to illustrate the technical solution of the utility model, to the greatest extent
The utility model is described in detail with reference to preferred embodiment for pipe, should be understood that and the foregoing is only this practicality newly
The embodiment of type, be not used to limit the scope of protection of the utility model, it is all it is of the present utility model spirit and
Within principle, any modification, equivalent substitution and improvements done etc., it should be included within the scope of protection of the utility model.
Claims (6)
1. one-stop site nucleic acid augmentation detection carrying case, including casing (1) and case lid, the case lid are covered on casing (1),
Characterized in that, pipettor (3), electronic broken instrument (4), centrifuge (5), metal bath (6), isothermal are provided with the casing (1)
Amplification fluorescent detector (7) and cell panel (8), the cell panel (8) respectively with the electronic broken instrument (4), centrifuge (5),
Metal bath (6) and isothermal duplication fluorescence detector (7) electrical connection；Be provided with multiple dividing plates (2) inside the casing (1), it is described every
Casing (1) is divided into space independent of each other, the pipettor (3), electronic broken instrument (4), centrifuge (5), metal by plate (2)
Bath (6), isothermal duplication fluorescence detector (7) and cell panel (8) are separately positioned in different spaces.
2. one-stop site nucleic acid augmentation detection carrying case according to claim 1, it is characterised in that the casing (1)
It is hinged with one end of case lid, the other end is detachably connected by buckle.
3. one-stop site nucleic acid augmentation detection carrying case according to claim 1, it is characterised in that the casing (1)
It is provided with handle (11).
4. one-stop site nucleic acid augmentation detection carrying case according to claim 1, it is characterised in that the dividing plate (2)
Surface be provided with air bag (9), offer gas vent on the air bag (9).
5. one-stop site nucleic acid augmentation detection carrying case according to claim 1, it is characterised in that the space it is interior
Portion's shape and pipettor (3), electronic broken instrument (4), centrifuge (5), metal bath (6), the isothermal for being arranged on the interior volume
The shape of amplification fluorescent detector (7) or cell panel (8) is engaged, and the surface of the dividing plate (2) is provided with liner.
6. one-stop site nucleic acid augmentation detection carrying case according to claim 5, it is characterised in that the liner uses
Foamed material is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720197375.0U CN206646095U (en) | 2017-03-02 | 2017-03-02 | One-stop site nucleic acid augmentation detection carrying case |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720197375.0U CN206646095U (en) | 2017-03-02 | 2017-03-02 | One-stop site nucleic acid augmentation detection carrying case |
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| Publication Number | Publication Date |
|---|---|
| CN206646095U true CN206646095U (en) | 2017-11-17 |
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ID=60280942
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720197375.0U Active CN206646095U (en) | 2017-03-02 | 2017-03-02 | One-stop site nucleic acid augmentation detection carrying case |
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| CN (1) | CN206646095U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108531390A (en) * | 2018-04-11 | 2018-09-14 | 张薇 | A kind of portable equipment that can be used for Site Detection nucleic acid |
-
2017
- 2017-03-02 CN CN201720197375.0U patent/CN206646095U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108531390A (en) * | 2018-04-11 | 2018-09-14 | 张薇 | A kind of portable equipment that can be used for Site Detection nucleic acid |
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