CN206594120U - A kind of capillary liquid chromatographic column temperature regulating device - Google Patents
A kind of capillary liquid chromatographic column temperature regulating device Download PDFInfo
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- CN206594120U CN206594120U CN201720251058.2U CN201720251058U CN206594120U CN 206594120 U CN206594120 U CN 206594120U CN 201720251058 U CN201720251058 U CN 201720251058U CN 206594120 U CN206594120 U CN 206594120U
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- capillary
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Abstract
A kind of capillary liquid chromatographic column temperature regulating device, is related to capillary liquid chromatographic column.Provided with radiator fan, hollow type capillary column support tube, capillary liquid chromatographic column, temperature control equipment and base, radiator fan is arranged at the top of hollow type capillary column support tube, the hollow type capillary column support tube periphery is covered with electric heating coating, thread groove is carved with outside hollow type capillary column support tube, the thread groove is used for fixed capillary liquid-phase chromatographic column, and the capillary liquid chromatographic column is with spiral wrap on hollow type capillary column support tube surface；Thermo-sensitive resistor layer is coated in the hollow type capillary column support tube periphery for being wound with capillary liquid chromatographic column, the temperature control equipment is provided with temperature sensor, display, the temperature sensor is used for the temperature for detecting thermo-sensitive resistor layer, the temperature signal output termination display of temperature sensor, by the temperature signal of display displays temperature sensor, hollow type capillary column support tube is arranged on base.
Description
Technical field
The utility model is related to capillary liquid chromatographic column, is filled more particularly, to a kind of capillary liquid chromatographic column temperature control
Put.
Background technology
Since the eighties in last century, with the development of chromatographic theory and correlation technique, people start gradually to recognize micro-
The advantage of piece-rate system, when the mobile phase flow rate that chromatographic run is used tends to micro updating or even nanoliter level, due to background solvent
Volume reduces, and can increase substantially detection sensitivity, so as to be adaptive to the analysis detection of micro-example.Simultaneously as
Required organic solvent amount is reduced in flow-reduction, unit run time, so as to limit the consumption of toxic solvent and save operation
Cost.In addition, in Tandem analysis technology online using liquid chromatography-mass spectrography, relatively low flow velocity can improve electron spray
The nebulization efficiency of ion gun is so as to further improve mass spectrographic detection sensitivity, it is adaptable to qualitative and quantitative point of complex sample
Analysis.
The important parameter that temperature is separated as liquid chromatogram, when directly affecting chromatogram separative efficiency and analysis operation
Between.By raising running temperature, mobile phase viscosity can be effectively reduced, so that under very fast flow conditions, system is still able to
Relatively low operating pressure is in, so as to largely improve chromatographic velocity.In addition, rise temperature can accelerate sample and exist
Mass-transfer efficiency between mobile phase and stationary phase, is conducive to obtaining preferable separating effect.
Traditional chromatographic column incubator can only support the capillary column of 15cm or so length mostly, and often for protein and
For the complex sample of the large biological molecules such as polypeptide, column length, which needs to reach that 50cm is even longer, to be allowed in single run
The resolution capability of people's satisfaction, this causes traditional column oven can not be applied to the research fields such as proteomics very well.Meanwhile, pass
The heating journey of system column oven is shorter, and mobile phase not fully reaches temperature equalization in column jecket, and influence separates reappearance, and these are not
Foot all governs the performance of traditional column oven function in actual use.
Chinese patent CN104034831A discloses a kind of overlength protein with temp auto-controlled, peptide separation capillary liquid phase color
Column system is composed, support is fixed on the middle part of shell；Populated capillary liquid chromatographic column is coiled on support, its post two
End is stretched out from the reserved aperture in the edge of shell respectively；After capillary is installed, insulation is filled between capillary column and shell
Material；Control panel is fixed on the middle part of shell, and with being placed in capillary column circle and close to the thermocouple of post, is fixed on
Temperature control module in capillary column circle and the semiconductor thermoelectric refrigeration piece connection as a column system shell part.The invention is
Controlling makees simple, cheap.Overlength capillary column is combined with temperature-controlling system, column temperature is raised, can effectively reduce mobile phase
Viscosity, reduces back-pressure, accelerates two alternate mass transfer rates, convenient, efficient to realize that sample is separated.
The content of the invention
The purpose of this utility model is that offer can be realized to the accurate fast of random length, especially overlength capillary column
A kind of capillary liquid chromatographic column temperature regulating device of fast temperature control.
The utility model is provided with radiator fan, hollow type capillary column support tube, capillary liquid chromatographic column, temperature control
Device and base, radiator fan are arranged at the top of hollow type capillary column support tube, outside the hollow type capillary column support tube
Week is covered with electric heating coating, and thread groove is carved with outside hollow type capillary column support tube, and the thread groove is used to fix capillary
Pipe liquid-phase chromatographic column, the capillary liquid chromatographic column is with spiral wrap on hollow type capillary column support tube surface；Twining
The hollow type capillary column support tube periphery cladding thermo-sensitive resistor layer of capillary liquid chromatographic column is wound with, the thermo-sensitive resistor layer is used
In the real-time column temperature of detection；The temperature control equipment is used to show real-time column temperature and controls to heat hollow type capillary column support tube
With the opening and closing of radiator fan, the temperature control equipment is provided with temperature sensor, display, and the temperature sensor is used
In the temperature of detection thermo-sensitive resistor layer, the temperature signal output termination display of temperature sensor is passed by display displays temperature
The temperature signal of sensor, hollow type capillary column support tube is arranged on base.
The hollow type capillary column support tube by heat good conductor be made, the hot good conductor may be selected from gold, silver,
Metal or alloy such as copper, iron etc., the external diameter of hollow type capillary column support tube can be 3~5cm.The electric heating coating can be used
Semiconductor thermoelectric film, infrared electrothermal film, magnetic induction Electric radiant Heating Film or other kinds of electric heating material, the thickness of electric heating coating can
It is described to be covered with electric heating coating for 0.1~5mm, can using spraying, paste or the method such as evaporation.
The thermo-sensitive resistor layer can be using RTD layer；The thermo-sensitive resistor layer is outer can be cased with being incubated sleeve, for maintaining
Steady temperature, it is described to be covered with thermo-sensitive resistor layer, it can use and miniature platinum thermal resistance sensing probe is welded on insulation sleeve lining；
The thickness for being incubated sleeve can be 0.5~10mm, and the insulation sleeve can be made up of resistant to elevated temperatures hot non-conductor, the high temperature resistant
Hot non-conductor may be selected from one kind in asbestos, polytetrafluoroethylene (PTFE), ceramic fiber cotton etc..
Populated capillary liquid chromatographic column is with spiral wrap on hollow type capillary column support tube surface.
Compared with the prior art, the utility model has following technique effect：
1) the utility model is used as heating skeleton using hollow type capillary column support tube so that capillary liquid chromatographic column
Spiral wrap can be taken on hollow type capillary column support tube surface, the body shared by the utility model is significantly reduced
Product, efficiently solves the heating problems of long column and overlength post, while allow many capillary liquid-phase chromatographic columns to heat simultaneously parallel,
Available in the chromatographic fractionation system of parallel multi-channel.
2) the spiral wrap structure that the utility model is used can effectively expand heating journey so that capillary liquid chromatography
Post can be with thermally equivalent in the range of full post.
3) radiator fan is arranged on hollow type capillary column support tube top by the utility model, using cross-ventilated side
Formula realizes quick reduction temperature.
4) electric heating coating of the present utility model is close to capillary liquid chromatographic column, and it is capillary to take contact heat transfer
Tubing string is heated so that heat transfer efficiency is higher.
5) the utility model uses platinum resistance temperature sensor, can precisely detect that capillary liquid chromatographic column is warm in real time
Degree, can accurately control capillary liquid chromatography running temperature, and excellent thermal insulation performance.
6) the utility model can be integrated in miniaturization chromatographic system, due to capillary column with spiral wrap in support tube
On, therefore support the temperature control of random length capillary chromatographic column, especially overlength capillary chromatographic column (being more than 50cm).
Brief description of the drawings
Fig. 1 is the structure composition schematic diagram of the utility model embodiment.
Embodiment
Following examples will be further described with reference to accompanying drawing to the utility model.
Referring to Fig. 1, the utility model embodiment is provided with radiator fan 1, hollow type capillary column support tube 2, capillary liquid
Phase chromatographic column 5, temperature control equipment 7 and base 6, radiator fan 1 are arranged on the top of hollow type capillary column support tube 2, described
The periphery of hollow type capillary column support tube 2 is coated with electric heating coating, and screw thread is carved with outside hollow type capillary column support tube 2
Groove, the thread groove is used for fixed capillary liquid-phase chromatographic column 5, and the capillary liquid chromatographic column 5 is with spiral wrap in
The empty surface of formula capillary column support tube 2；In the periphery of hollow type capillary column support tube 2 for being wound with capillary liquid chromatographic column 5
Thermo-sensitive resistor layer 3 is covered with, the thermo-sensitive resistor layer 3 is used to detect real-time column temperature；The temperature control equipment 7 is used to show in real time
Column temperature simultaneously controls to heat the opening and closing of hollow type capillary column support tube 2 and radiator fan 1, the temperature control equipment 7
Provided with temperature sensor, display, the temperature sensor is used for the temperature for detecting thermo-sensitive resistor layer 3, the temperature of temperature sensor
Signal output termination display is spent, by the temperature signal of display displays temperature sensor, hollow type capillary column support tube 2 is pacified
On base 6.
The hollow type capillary column support tube 2 by heat good conductor be made, the hot good conductor may be selected from gold, silver,
Metal or alloy such as copper, iron etc., the external diameter of hollow type capillary column support tube 2 can be 3~5cm.The electric heating coating can be adopted
With semiconductor thermoelectric film, infrared electrothermal film, magnetic induction Electric radiant Heating Film or other kinds of electric heating material, the thickness of electric heating coating
Can be 0.1~5mm, it is described to be covered with electric heating coating, can be using methods such as spraying, stickup or evaporations.
The thermo-sensitive resistor layer 3 can be using RTD layer；It can be cased with being incubated sleeve 4 outside the thermo-sensitive resistor layer 3, for tieing up
Steady temperature is held, it is described to be covered with thermo-sensitive resistor layer 3, it can use and miniature platinum thermal resistance sensing probe is welded on insulation sleeve 4
Inwall；The thickness for being incubated sleeve 4 can be 0.5~10mm, and the insulation sleeve 4 can be made up of resistant to elevated temperatures hot non-conductor, institute
State resistant to elevated temperatures hot non-conductor and may be selected from one kind in asbestos, polytetrafluoroethylene (PTFE), ceramic fiber cotton etc..
Populated capillary liquid chromatographic column is with spiral wrap on hollow type capillary column support tube surface.
Specific embodiment given below.
An external diameter 50mm is processed, thickness 3mm, high 150mm copper hollow cylinder, surface are gone out to solid using lathe process
Determine the screw thread of capillary liquid chromatographic column.It is 1m, 365 μm of external diameter, the capillary liquid chromatographic column that 100 μm of internal diameter to take a length
Support tube surface is wrapped in by threaded line.
Vapour deposition process is used to plate semiconductor thermoelectric film of a layer thickness for 0.5mm on support tube surface, and in support
Cylinder upper and lower ends pick out wire respectively.
It is 50.3mm that the ceramic fiber cotton for being 3mm with thickness, which makes an internal diameter, high 120mm insulation sleeve, in sleeve
Wall is pasted with metal platinum sensor probe, and picks out respective wire.The above-mentioned support tube for being tied with capillary column is passed through and protected
It is fixed on after warm sleeve on base, capillary column is imported and exported and stretched out respectively from sleeve upper and lower ends.
Radiator fan is installed with screw and bolt arrangement at the top of support tube, by its wire together with Electric radiant Heating Film wire, platinum electricity
Resistance sensor conductor is connected on the corresponding pin of temperature control equipment together.
The heater switch opened in chromatographic system equilibrium process in temperature control equipment starts to capillary liquid chromatography
Post is heated, while real-time column temperature can be shown by obtaining display screen on temperature control equipment, is heated to after assigned temperature disconnecting heating
Switch enters insulating process, and opening fan swicth when need to reduce temperature carries out forced convertion.
Claims (5)
1. a kind of capillary liquid chromatographic column temperature regulating device, it is characterised in that supported provided with radiator fan, hollow type capillary column
Cylinder, capillary liquid chromatographic column, temperature control equipment and base, radiator fan are arranged on hollow type capillary column support tube top
Portion, the hollow type capillary column support tube periphery is covered with electric heating coating, is carved with outside hollow type capillary column support tube
Thread groove, the thread groove is used for fixed capillary liquid-phase chromatographic column, and the capillary liquid chromatographic column is existed with spiral wrap
Hollow type capillary column support tube surface；Wrapped in the hollow type capillary column support tube periphery for being wound with capillary liquid chromatographic column
Thermo-sensitive resistor layer is covered, the thermo-sensitive resistor layer is used to detect real-time column temperature；The temperature control equipment is used to show real-time column temperature
And control to heat the opening and closing of hollow type capillary column support tube and radiator fan, the temperature control equipment is provided with temperature
Sensor, display, the temperature sensor are used for the temperature for detecting thermo-sensitive resistor layer, the temperature signal output of temperature sensor
Display is terminated, by the temperature signal of display displays temperature sensor, hollow type capillary column support tube is arranged on base.
2. a kind of capillary liquid chromatographic column temperature regulating device as claimed in claim 1, it is characterised in that hollow type capillary column branch
The external diameter for supportting cylinder is 3~5cm.
3. a kind of capillary liquid chromatographic column temperature regulating device as claimed in claim 1, it is characterised in that the electric heating coating
Thickness is 0.1~5mm.
4. a kind of capillary liquid chromatographic column temperature regulating device as claimed in claim 1, it is characterised in that the thermo-sensitive resistor layer is outer
It is cased with being incubated sleeve.
5. a kind of capillary liquid chromatographic column temperature regulating device as claimed in claim 4, it is characterised in that the thickness of insulation sleeve is
0.5~10mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720251058.2U CN206594120U (en) | 2017-03-15 | 2017-03-15 | A kind of capillary liquid chromatographic column temperature regulating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720251058.2U CN206594120U (en) | 2017-03-15 | 2017-03-15 | A kind of capillary liquid chromatographic column temperature regulating device |
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| Publication Number | Publication Date |
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| CN206594120U true CN206594120U (en) | 2017-10-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201720251058.2U Active CN206594120U (en) | 2017-03-15 | 2017-03-15 | A kind of capillary liquid chromatographic column temperature regulating device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024065861A1 (en) * | 2022-09-26 | 2024-04-04 | 台湾积体电路制造股份有限公司 | Furnace tube device |
-
2017
- 2017-03-15 CN CN201720251058.2U patent/CN206594120U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024065861A1 (en) * | 2022-09-26 | 2024-04-04 | 台湾积体电路制造股份有限公司 | Furnace tube device |
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