CN206512226U - A kind of cell culture apparatus - Google Patents
A kind of cell culture apparatus Download PDFInfo
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- CN206512226U CN206512226U CN201720140503.8U CN201720140503U CN206512226U CN 206512226 U CN206512226 U CN 206512226U CN 201720140503 U CN201720140503 U CN 201720140503U CN 206512226 U CN206512226 U CN 206512226U
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- cell culture
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Abstract
The utility model discloses a kind of cell culture apparatus, including a substrate, one Support Channel, two microfluidic channels, length direction of the Support Channel along the substrate is centrally disposed, two microfluidic channels are separately positioned on the both sides of Support Channel, at least one position includes leakproof unit in the top surface of the junction of Support Channel and microfluidic channel or bottom surface, leakproof unit is for preventing timbering material from Support Channel flow to microfluidic channel, the utility model can realize the three dimensional viewing and measure of cell culture, furthermore, the top surface or bottom surface of passage and the boundary member of microfluidic channel between being extended to due to leakproof unit, therefore it can prevent cell from being reacted with other structures, it is enable to carry out the situation that accurate observation quantifies cell culture, interaction between the reaction of cell and cell.
Description
Technical field
The utility model is related to field of cell culture, more particularly to one kind can mass produce cell and can be exactly
The device of observation culture cell.
Background technology
The movement of cell is migration of the organism individual cells for various physics, chemistry or biological respinse, and
Deeply participate in the physiological phenomenon of the various diseases of human body, such as AIDS, bacterium infection, artery sclerosis, arthritis, periodontitis, ox
The diseases such as psoriasis, cancer, multiple sclerosis, male sterility.It is based on however, the cell evaluation of most conventional is determined
The signaling of two-dimensional space, therefore can not accurately help to observe the movement of cell, and because cell is actually existed in three
Dimension space, therefore conventional determining result is different from being really phenomenon.
Utility model content
There is provided a kind of observation based on three dimensions for the defect for aiming to overcome that prior art of the present utility model
And the device of signaling is determined, what the technical scheme of the device was realized in.
A kind of cell culture apparatus, including substrate, Support Channel, two microfluidic channels, the support lead to
Length direction of the road along the substrate is centrally disposed, and the Support Channel is the passage of timbering material flowing, described two micro-
Fluid passage is separately positioned on the both sides of the Support Channel, and the microfluidic channel is the passage flowed with cell, the branch
At least one position includes leakproof unit, the leakproof list in the top surface of the junction of frame passage and microfluidic channel or bottom surface
Member is that, for preventing timbering material from Support Channel flow to microfluidic channel, the height of the leakproof unit is that support leads to
The 20%~80% of road and microfluidic channel height, the leakproof unit is the length along the Support Channel and microfluidic channel
What the boundary in degree direction was continuously set.
The top of the Support Channel and microfluidic channel is all semicircular, the handing-over on the border of both semi-circular upper portions
Locate the leakproof unit described in formation, the end of the leakproof unit is sharp.
The Support Channel and the microfluidic channel are repeated and alternate mode is set.
The end of the microfluidic channel is away from the Support Channel.
The Support Channel is formed by injecting fluid material gel or formed by curing materials.
The material of the Support Channel is diatom salt, collagen, peptide, fibrin, Hyaluronic Acid, PHEMA, PVA, PEG, PEO,
Two kinds in PEGDA, gelatin, matrigel, PLLA, carboxymethyl cellulose, SAP, PHEMA-MMA, glucan and chitosan or more
The mixture of many materials.
The beneficial effects of the utility model are：The utility model can realize the three dimensional viewing and measure of cell culture, then
Person, the top surface or bottom surface of passage and the boundary member of microfluidic channel between being extended to due to leakproof unit, therefore can prevent
Cell and other structures are reacted, be enable to carry out accurate observation quantify the situation of cell culture, the reaction of cell and
Interaction between cell, further, since Support Channel and microfluidic channel can be set, so as to subtract in many ways
Few observation and the time for determining cell.
Brief description of the drawings
The utility model is described in further detail with reference to the accompanying drawings and detailed description, embodiment is only this reality
With new one embodiment, for those of ordinary skill in the art, without having to pay creative labor, go back
Other embodiments can be obtained with reference to the accompanying drawings, also within protection scope of the present invention.
Fig. 1 is the structural representation of the utility model cell culture apparatus；
Fig. 2 is the I-I diagrammatic cross-sections in the utility model cell culture apparatus；
Fig. 3 is the schematic diagram after the utility model cell culture apparatus timbering material formation Support Channel.
Reference：1- Support Channels, 10- Support Channel entrances, the outlet of 11- Support Channels, 100- substrates, 2- microfluids
Passage, 20- microfluidic channel inlets, the outlet of 21- microfluidic channels, 3- leakproof units, 4- timbering materials.
Embodiment
As shown in Figure 1 to Figure 3, a kind of cell culture apparatus disclosed in the utility model：Including a substrate 100, one
Individual Support Channel 1, two microfluidic channels 2.
Support Channel 1 and two microfluidic channels 2 are all disposed within basic 100, and the material of substrate 100 is that by micro-
The material of fluidic structures, can shift a small amount of any material.
Two microfluidic channels 2 are the passages of cell flowing, are arranged on the both sides of substrate 100, Support Channel 1 is provided in
The centre of 2 microfluidic channels.
Support Channel 1 is set along the Center Length direction of substrate 100, be provided with the upper end of Support Channel 1 into
Mouthfuls 10, the lower end of Support Channel 1 is provided with window 11, in addition, be also respectively equipped with the front and back end of microfluidic channel entrance and
Outlet.
Extracellular matrix is constituted after support when the flowing of timbering material 4 is formed, the advantage of microflow control technique is exactly to safeguard and thin
The three-dimensional reaction of born of the same parents' assessment of flow simulation, this timbering material 4 must flow in Support Channel 1, it is impossible to leak into microfluid
Passage 2.In addition, support is a kind of liquid, the described Support Channel 1 of cured rear formation is injected in specific position.
The material of Support Channel 1 can a kind of solidification material directly formed, can also through inject fluid solidify to form
, in order to solidify support, can again predetermined time with heating, the method for chemical mixing, or with the method for radiant light
Deng the material of Support Channel 1 is diatom salt, collagen, peptide, fibrin, Hyaluronic Acid, PHEMA, PVA, PEG, PEO, PEGDA, bright
Two or more materials in glue, matrigel, PLLA, carboxymethyl cellulose, SAP, PHEMA-MMA, glucan and chitosan
Mixture, in addition, the material that timbering material can also directly be extracted from body, such as cell, cell cluster or living tissue or
It is its mixture.
In the conventional technology, timbering material 4, which is hampered by microelectrode array, need to be fixed on a specific position, and cell
Also it can be reacted prior to microelectrode array rather than timbering material 4, and in the utility model, by Support Channel 1 and miniflow
The boundary member of body passage 2 sets leakproof unit 3, solves above-mentioned problem, deletes above-mentioned microelectrode array, by branch
The top surface of the border junction of frame passage 1 and microfluidic channel 2 or bottom surface form the leakproof unit of a predetermined length to make up
Remove the defect of microelectrode array.
When timbering material 4 flows into Support Channel 1 for the first time, due to the cohesive strength of support in itself, the two ends of support lead to
Road is likely to form the shape of depression, over time, starts to disperse between both sides, forms prominent shape, is formed
Shape depends on the surface contact angle of the passage.According to said process, the support with the surface of leakproof unit 3 due to opening
Power can prevent cell from being leaked into from microfluidic channel 2 in Support Channel 1.
The height of leakproof unit 3 is the 20%~80% of Support Channel 1 and the height of microfluidic channel 2, and leakproof unit 3 is edge
What the boundary of Support Channel 1 and the length direction of microfluidic channel 2 was continuously set.
The top of Support Channel 1 and microfluidic channel 2 is all semicircular, the junction on the border of both semi-circular upper portions
Described leakproof unit 3 is formed, the end of leakproof unit is sharp.
Support Channel 1 and microfluidic channel 2 are repeated and alternate mode is set.
The end of microfluidic channel 2 is away from Support Channel 1.
Claims (5)
1. a kind of cell culture apparatus, it is characterised in that：The cell culture apparatus includes：One substrate, Support Channel,
Two microfluidic channels, length direction of the Support Channel along the substrate is centrally disposed, and the Support Channel is support
The passage of material flowing, described two microfluidic channels are separately positioned on the both sides of the Support Channel, the microfluidic channel
It is the passage flowed with cell, at least one portion in the top surface of the junction of the Support Channel and microfluidic channel or bottom surface
Position include leakproof unit, the leakproof unit be for preventing timbering material from Support Channel flow to microfluidic channel,
The height of the leakproof unit is the 20%~80% of Support Channel and microfluidic channel height, and the leakproof unit is along institute
State what the boundary of Support Channel and the length direction of microfluidic channel was continuously set.
2. cell culture apparatus according to claim 1, it is characterised in that：The Support Channel and microfluidic channel it is upper
Portion is all semicircular, and the junction on the border of both semi-circular upper portions forms described leakproof unit, the leakproof unit
End is sharp.
3. cell culture apparatus according to claim 1, it is characterised in that：The Support Channel and the microfluidic channel
Repeat and alternate mode is set.
4. cell culture apparatus according to claim 1, it is characterised in that：The end of the microfluidic channel is away from institute
State Support Channel.
5. cell culture apparatus according to claim 1, it is characterised in that：The Support Channel is by injecting fluid material
Gel is formed or formed by curing materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720140503.8U CN206512226U (en) | 2017-02-16 | 2017-02-16 | A kind of cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720140503.8U CN206512226U (en) | 2017-02-16 | 2017-02-16 | A kind of cell culture apparatus |
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| CN206512226U true CN206512226U (en) | 2017-09-22 |
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| CN201720140503.8U Active CN206512226U (en) | 2017-02-16 | 2017-02-16 | A kind of cell culture apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112300940A (en) * | 2020-10-30 | 2021-02-02 | 大连医科大学 | Periodontal soft tissue bionic chip constructed based on microfluidic technology and application thereof |
-
2017
- 2017-02-16 CN CN201720140503.8U patent/CN206512226U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112300940A (en) * | 2020-10-30 | 2021-02-02 | 大连医科大学 | Periodontal soft tissue bionic chip constructed based on microfluidic technology and application thereof |
| CN112300940B (en) * | 2020-10-30 | 2023-06-16 | 大连医科大学 | Periodontal soft tissue bionic chip constructed based on microfluidic technology and application thereof |
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