CN206504991U - A kind of air microbe tests consersion unit - Google Patents
A kind of air microbe tests consersion unit Download PDFInfo
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- CN206504991U CN206504991U CN201720045320.8U CN201720045320U CN206504991U CN 206504991 U CN206504991 U CN 206504991U CN 201720045320 U CN201720045320 U CN 201720045320U CN 206504991 U CN206504991 U CN 206504991U
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Abstract
The utility model discloses a kind of air microbe experiment consersion unit, for the aerial reactions change of microorganisms.The equipment includes reaction cabin, empty gasification generator and air sampler, is connected on the side wall of reaction cabin provided with air inlet with empty gasification generator, relative opposite side wall is connected provided with gas outlet with air sampler;Compartment roof, which is provided with fluorescent lamp and uviol lamp, cabin, is provided with fan and Temperature Humidity Sensor;The volume of the reaction cabin is 150 500 liters, and inwall scribbles hydrophobic coating.The hydrophobic surface of reaction cabin, fan disturbance, can largely ensure that microorganism is in air;Its volume can meet different open-assembly times and subsequent acquisition detection needs;The various devices of nacelle over-assemble can change the different condition of the air reaction of microorganism, and can in real time control, detect in real time, help to further appreciate that the aerial propagation characteristic of microorganism especially pathogenic bacteria.
Description
Technical field
The utility model belongs to aerosol art, and in particular to reactions change is set in atmosphere for a kind of microorganisms
It is standby, the device of the exposure reaction of the aerial activity of microorganisms, appeal and change in physical properties.
Background technology
Air is the important medium of microorganisms spreading, many human disease bacterium air-borne transmissions, can produce communicable diseases and break out
It is popular.Microorganism in air includes bacterium, fungi, virus, Richettsia, mycoplasma and Chlamydia etc..These microorganisms
There are many pathogen for causing infection in respiratory system in grain, such as haemophilus influenzae, legionella pneumophilia, influenza virus.
The outburst that produces communicable diseases, such as tuberculosis, measles, Q heat, pig stream are easy in some special occupation environment, crowd massing place etc.
Sense, anthrax, legionella pneumophilia disease etc..Research shows that air borne is the important channel that pathogen is propagated.
Microorganism in atmosphere can by aerosol transmission, due to the diversity of species, its survival ability in the environment,
Virulence change performance is different, therefore the change of microbial aerosol is also in variation.The particulate matter of different-grain diameter is in air
In propagation characteristic and apart from difference.For example, the propagation distance of small size particle is farther out, the propagation distance of large-size particles thing
It is nearer.Influenceed in addition, microorganism is propagated in atmosphere by a variety of environmental factors, including temperature, humidity, electromagnetic radiation and air
Chemical group is graded.Temperature is to influence the key factor of microbial survival.The influence radiated to microbial activity is relevant with wavelength, ripple
The radiation of length, energy by force, such as ultraviolet radioactive can destroy the DNA of pathogen;Long wave and visible ray can influence cell center line
The generation of cytochromes in plastochondria.Outside atmosphere chemical compositions are complex, and the mechanism of the influence to microorganism is also more
It is complicated.
Therefore, it is necessary to which under controllable environmental factor, to microorganism, change includes its activity for a long time in atmosphere
Studied.This air borne to evaluating microorganism, it is very to study its aerial physical change and biological decay etc.
It is necessary.
Existing air microbe research device is most of it cannot be guaranteed that microorganism stops in atmosphere for a long time, Er Qiehuan
Border factor poor controllability, open-assembly time is short, it is impossible to which the change that pathogenic bacteria occur in atmosphere is apparent from.Microorganism
It there occurs that what it is not immediately clear actually in atmosphere, the aerial pathogenic change of such as microorganism and propagation understand
Not enough.The microbial exposure device reported in document is in addition, there will be, general volume is larger, cost is high, and is difficult to thoroughly sterilize,
Easily cause the cross pollution of many experiments reaction.Also study air microbe being transferred in liquid phase and reacted, design liquid phase
Exposure reaction, it is impossible to react its aerial truth.
Utility model content
Main purpose of the present utility model is a kind of equipment of microorganisms of design reactions change in atmosphere, is micro- life
The air borne of thing aerosol provides an ideal experiment place, and can control in real time every response parameter (humiture,
Illumination, open-assembly time, ensure its be in air it is medium), detect microorganism oxidative damage and drying effect, study its occur,
Physical change and biological decay in propagation etc..
The technical solution of the utility model is as follows:
A kind of air microbe tests consersion unit, including reaction cabin, empty gasification generator and air sampler, in reaction
The side wall in cabin is connected provided with air inlet with empty gasification generator, and relative opposite side wall is provided with gas outlet and air sampling
Device is connected;Compartment roof, which is provided with fluorescent lamp and uviol lamp, cabin, is provided with fan and Temperature Humidity Sensor;Wherein:It is described anti-
The volume for answering cabin is 150-500 liters, and cabin inner walls scribble hydrophobic coating.
The nacelle of the reaction cabin is preferably cuboid, is made using stainless steel plate, and cabin inner walls scribble one layer of hydrophobic painting
Layer, to reduce absorption and deposition of the microbe granular in inwall.Coating material is preferably silicon nano-resin, and it also has high temperature resistant
With the characteristic of ultraviolet.In one embodiment of the present utility model, the size of reaction nacelle is 700mm × 500mm × 500mm
(length × width × height), volume is 175 liters, can meet requirement of the bioaerosol sampler to volume of air.For example, using
During BioSampler samplers, sampling flow is 12.5L/min, can gather 10min;Sampled using BioStage and Andersen
During device, sampling flow is 28.3L/min, can gather 5min.
Empty gasification generator is connected with the air inlet of reaction cabin, and aerosolized microorganism is passed through into nacelle.Further,
Moisture in drying tube, reduction microbe granular can be added between empty gasification generator and air inlet, reduces particle diameter, mitigates
Quality, reduces its free settling.The empty gasification generator can use respiration bottle, and carrier gas is done using nitrogen, will be aerosolized
Microbe granular is passed through reaction cabin.
The gas outlet of reaction cabin is connected with air sampler, and the air sampler is biological aerosol sampler, collection
Reacted microorganism, gained sample can be used for the indexs such as detection activity, oxidative damage and drying effect.Meanwhile, the air
Experiment on Microbiology consersion unit can also further comprise concentration detection apparatus, and such as optical particle counter (OPC) or ultraviolet-gas are molten
Glue particle size analyzer (UV-APS) etc., the gas outlet of reaction cabin is connected with concentration detection apparatus, and micro- life in cabin can be detected in real time
The concentration of composition granule.
Installed in the fluorescent lamp and uviol lamp of compartment roof, illumination and ultraviolet radioactive are respectively configured to provide.Uviol lamp may be used also
For for cabin sterilization.
The fan can use simple ceiling fan or scavenger fan, for disturbing the air-flow in cabin, reduce microorganism
The sedimentation of grain, increases its aerial stop and reaction time.
The Temperature Humidity Sensor is preferred to use split type sensor, and its probe is passed through cabin interior, for monitoring cabin
Interior temperature and humidity, and display pannel is suspended on the outside of cabin, is easy to real time readouts.
Further, back up ventilation mouthful is provided with the cabin side wall of reaction cabin, can be led to by back up ventilation mouthful into cabin
Enter various reaction gases, such as O3, for carrying out experiment of the gas to air microbe activity influence.When not in use, plug can be used
Block.
Further, blower fan or ventilation fan can be set in cabin bottom, increases air agitation.
Air microbe experiment consersion unit of the present utility model when in use, first passes through sky gasification generator by aerosol
The microbe granular of change is passed through in reaction cabin through air inlet, provides various reaction conditions by reaction cabin for microorganism, for example:Day
Light lamp provides visible ray illumination, and uviol lamp provides the gas disturbance situation in ultraviolet radioactive, fan control cabin, temperature and humidity sensing
Reaction temperature and humidity in device control cabinet room, back up ventilation mouthful can be passed through the gases such as vapor, ozone.It is micro- after the completion of reaction
Biology is gathered by gas outlet by air sampler, carries out coherent detection.
The beneficial effects of the utility model:The change that the utility model occurs in atmosphere for microorganisms first is provided
Research meanses and method, the volume of reaction cabin can meet different open-assembly times and detect needs with subsequent acquisition.Secondly, this practicality
It is new by that in the various devices of nacelle over-assemble, can change the different condition of the air reaction of microorganism, and can control in real time, it is real
When detect.In addition, the exposing device of research and development has hydrophobic surface, fan disturbance, it can largely ensure that microorganism is in
In atmosphere.The research meanses and method help to further appreciate that the aerial propagation of microorganism especially pathogenic bacteria is special
Property.
Brief description of the drawings
Fig. 1 air microbes test reaction cabin stereogram, wherein:1 is reaction nacelle;2 be air inlet;3-1 is daylight
Lamp;3-2 is uviol lamp;4 be fan;5 be Temperature Humidity Sensor;6 be gas outlet;7 be back up ventilation mouthful.
Embodiment
Below in conjunction with the accompanying drawings, the utility model is further illustrated by embodiment.
Using shown in Fig. 1 air microbe experiment reaction cabin microorganisms it is aerial activity, appeal and
Change in physical properties, air microbe experiment reaction cabin includes reaction nacelle 1, and air inlet is provided with the side of reaction nacelle 1
2, relative opposite side is provided with gas outlet 6, and compartment roof sets fluorescent lamp 3-1 and uviol lamp 3-2, and is hung with fan 4, cabin
Interior be provided with Temperature Humidity Sensor, bulkhead also leaves back up ventilation mouthful 7.
The reaction nacelle 1 is cuboid, is made using stainless steel plate, and its size is 700mm × 500mm × 500mm (long
× wide × high), volume is 175 liters, can meet requirement of the bioaerosol sampler to volume of air.For example, using
During BioSampler samplers, sampling flow is 12.5L/min, can gather 10min;Sampled using BioStage and Andersen
During device, sampling flow is 28.3L/min, can gather 5min.
Air inlet 2 is located at the left side in cabin, internal diameter 50mm.Air inlet 2 can be connected with empty gasification generator, will be aerosolized
Microorganism be passed through nacelle.Gas outlet 6 is located at the right side in cabin, internal diameter 50mm.Gas outlet 3 is connected with sampler, collection exists
Reacted microorganism in cabin, gained sample can be used for the indexs such as detection activity, oxidative damage and drying effect;May be used also simultaneously
It is connected with concentration detection apparatus, such as optical particle counter (OPC) or ultraviolet-aerosol particle size analyzer (UV-APS), can
The concentration of microbe granular in cabin is detected in real time.
Each one of fluorescent lamp 3-1 and uviol lamp 3-2 is installed in compartment roof, for providing illumination and ultraviolet radioactive.It is ultraviolet
Lamp 3-2 can be additionally used in for cabin sterilization.
Fan 4 uses simple ceiling fan, and flabellum diameter 420mm, for disturbing the air-flow in cabin, reduces microbe granular
Sedimentation, increases its aerial stop and reaction time.
Temperature Humidity Sensor 5 is split type, and probe is passed through cabin interior, for monitoring the humiture in cabin, error model
Enclose ± 2%;Display pannel is suspended on the outside of cabin, is easy to real time readouts.
In cabin side, wall leaves a back up ventilation mouthful 7, its internal diameter 20mm, and various reaction gases, example can be passed through into cabin
Such as O3, for carrying out experiment of the gas to air microbe activity influence.When not in use, it can be blocked with plug.
Concrete operations are as follows:
1) respiration bottle (Collison Nebulizer) is connected with air inlet 2, uses nitrogen (N2) carrier gas is done, gas is molten
The microbe granular of gel is passed through reaction cabin;
2) by the switch or condition of each part in control cabinet room, differential responses parameter is provided for microorganism, including but
It is not limited to:
A. by being turned on and off fluorescent lamp 3-1, visible ray illumination is controlled;
B. by being turned on and off uviol lamp 3-2 (uviol lamp can not simultaneously be opened with fluorescent lamp), ultraviolet radioactive is controlled;
C. situation is disturbed by the gas being turned on and off in fan 4, control cabinet room;
D. cabin indoor temperature is controlled by changing ambient temperature, reading can be carried out by Hygrothermograph 5;
E. the water vapour of atomization is passed through into cabin by back up ventilation mouthful 7, changes cabin indoor humidity, can be by Hygrothermograph
5 carry out reading;
F. other gases, such as ozone (O are passed through into cabin by back up ventilation mouthful 73), enter in atmosphere with microorganism
Row reaction.
3) sampler is connected with gas outlet 6, the microorganism after collection reaction completion.Using BioStage or
Andersen samplers samples, can directly be cultivated, the concentration of Bacterial diversity in detection cabin.Use BioSampler
Samplers sample, obtains liquid sample, can carry out molecular Biological Detection, detects activity, oxidative stress and the drying of microorganism
Effect etc..
4) corpuscular counter is connected with gas outlet 6, the concentration of cabin indoor accelerated test can be detected in real time.
Claims (10)
1. a kind of air microbe tests consersion unit, it is characterised in that the equipment includes reaction cabin, empty gasification generator and sky
Gas sampling, is connected, relative opposite side wall is provided with the side wall of reaction cabin provided with air inlet with empty gasification generator
Gas outlet is connected with air sampler;Compartment roof is provided with fluorescent lamp and uviol lamp, cabin to be passed provided with fan and humiture
Sensor;Wherein:The volume of the reaction cabin is 150-500 liters, and cabin inner walls scribble hydrophobic coating.
2. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that the hydrophobic coating is silicon nanometer
Resinous coat.
3. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that the empty gasification generator is produced
Aerosolized microorganism be passed through by air inlet in reaction cabin;The air sampler is biological aerosol sampler.
4. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that the empty gasification generator is with entering
Drying tube is provided between gas port.
5. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that the equipment also includes being used in fact
When detection cabin in microbe granular concentration concentration detection apparatus, the gas outlet phase of the concentration detection apparatus and the reaction cabin
Even.
6. air microbe as claimed in claim 5 tests consersion unit, it is characterised in that the concentration detection apparatus is light
Learn corpuscular counter or ultraviolet-aerosol particle size analyzer.
7. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that the fan is ceiling fan or ventilation
Fan.
8. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that the Temperature Humidity Sensor is to divide
Body formula sensor, it, which is popped one's head in, is located at cabin interior, and display pannel is located at outside cabin.
9. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that also set in reaction cabin cabin bottom
It is equipped with blower fan or ventilation fan.
10. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that the cabin side wall of reaction cabin is set
There is back up ventilation mouthful.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106769811A (en) * | 2017-01-16 | 2017-05-31 | 北京大学 | A kind of air microbe tests consersion unit and application |
CN109407629A (en) * | 2018-09-11 | 2019-03-01 | 广州市赛特检测有限公司 | A kind of pathogen on-line monitoring system and method |
CN111366512A (en) * | 2020-04-21 | 2020-07-03 | 中南大学 | Method for researching propagation mechanism of pathogen-carrying particles in passenger room of high-speed train |
CN111487168A (en) * | 2020-04-21 | 2020-08-04 | 中南大学 | System and method for testing propagation rule of pathogen-carrying particulate matters in passenger room of high-speed train |
-
2017
- 2017-01-16 CN CN201720045320.8U patent/CN206504991U/en active Active
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106769811A (en) * | 2017-01-16 | 2017-05-31 | 北京大学 | A kind of air microbe tests consersion unit and application |
CN109407629A (en) * | 2018-09-11 | 2019-03-01 | 广州市赛特检测有限公司 | A kind of pathogen on-line monitoring system and method |
CN111366512A (en) * | 2020-04-21 | 2020-07-03 | 中南大学 | Method for researching propagation mechanism of pathogen-carrying particles in passenger room of high-speed train |
CN111487168A (en) * | 2020-04-21 | 2020-08-04 | 中南大学 | System and method for testing propagation rule of pathogen-carrying particulate matters in passenger room of high-speed train |
CN111366512B (en) * | 2020-04-21 | 2022-02-22 | 中南大学 | Method for researching propagation mechanism of pathogen-carrying particles in passenger room of high-speed train |
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