CN206474190U - A kind of micro-fluidic chip suitable for multielectrode sensor - Google Patents
A kind of micro-fluidic chip suitable for multielectrode sensor Download PDFInfo
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- CN206474190U CN206474190U CN201720130759.0U CN201720130759U CN206474190U CN 206474190 U CN206474190 U CN 206474190U CN 201720130759 U CN201720130759 U CN 201720130759U CN 206474190 U CN206474190 U CN 206474190U
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Abstract
The utility model discloses a kind of micro-fluidic chip suitable for multielectrode sensor, it is related to micro fluidic chip technical field.The utility model is by using the n reactive tank including being arranged at Same Latitude, runner, the micro-fluidic chip of detect tank and reservoir, due to being provided with the controlling switch open in usual whether control runner circulates at the inlet of the 1st reactive tank and the first liquid outlet of preceding (n 1) individual reactive tank, and the normally closed type controlling switch whether control runner circulates is provided with the second liquid outlet of preceding (n 1) individual reactive tank, therefore, can by control normally closed type controlling switch and controlling switch open in usual be turned on and off control the quantity of reactive tank needed to use, so as to reach the purpose for the detection for carrying out different aspect to same sample to be detected simultaneously, simplify testing process, realize the rapid of parting detection, it is miniaturized and integrated.
Description
Technical field
The utility model belongs to micro fluidic chip technical field, more particularly to a kind of miniflow suitable for multielectrode sensor
Control chip.
Background technology
Microfluidic chip technology is sample preparation, reaction, separation, detection during biological, chemistry, medical analysis etc.
Basic operation unit is integrated on the chip of one piece of micro-meter scale, is automatically performed the technology of analysis overall process.Micro-fluidic chip is
Microflow control technique realize main platform, its device characteristic be mainly its accommodate fluid resulting structure (runner, reative cell or its
Its functional part) at least on a latitude be micron order yardstick.Due to micron-sized structure, fluid shows and produced wherein
The property different from macro-scale, therefore develop the performance that the analysis of uniqueness is produced.At present, microfluidic chip technology
Application be concentrated mainly in terms of nucleic acid separation and quantitative, DNA sequencing, gene mutation and analysis of gene differential expression.
Micro-fluidic chip generally requires cooperation electrochemica biological sensor (electrode sensor) and buffer solution is used.Detection
When, by electrode sensor insert micro-fluidic chip reactive tank in, by sample to be detected and the mixed detection liquid pump of buffer solution
Enter micro-fluidic chip so that detection liquid is fully hybridized with electrode sensor, passes through the detection unit pair in micro-fluidic chip
Detection liquid after hybridization, which carries out detection, can complete detection process.
However, existing micro-fluidic chip generally only includes a reactive tank, it can only be treated with reference to an electrode sensor
Detect that sample carries out the detection of single aspect, it is impossible to many detections are carried out to sample to be detected simultaneously according to the actual requirements.
Detection to carry out multiple different aspects to sample to be detected, then need to use multiple micro-fluidic chips, complex operation and into
This is higher.
In summary, existing micro-fluidic chip, which exists, to ask simultaneously many detections of sample to be detected progress
Topic.
Utility model content
The purpose of this utility model is to provide a kind of micro-fluidic chip suitable for multielectrode sensor, it is intended to solve existing
With the presence of micro-fluidic chip can not simultaneously to sample to be detected carry out many detections the problem of.
The utility model is achieved in that a kind of micro-fluidic chip suitable for multielectrode sensor, for it is described
Electrode sensor and default buffer solution are used cooperatively to be detected to sample to be detected, and the micro-fluidic chip includes setting
In n reactive tank, runner, detect tank and the reservoir of Same Latitude, n is the integer more than 1；
The n reactive tank is connected since the 1st reactive tank according to the first liquid outlet of previous reactive tank by runner
The mode for leading to the inlet of latter reactive tank is sequentially communicated to n-th of reactive tank, the first liquid outlet of n-th of reactive tank
And the second liquid outlet of preceding (n-1) individual reactive tank is connected by runner with the inlet of the detect tank, the detect tank
Liquid outlet connected by runner with the inlet of reservoir and the 1st reactive tank, the inlet of the 1st reactive tank
For the inlet of the micro-fluidic chip；
The inlet of 1st reactive tank and it is described before (n-1) individual reactive tank the first liquid outlet at be provided with
The controlling switch open in usual that whether circulates of control runner, it is described before (n-1) individual reactive tank the second liquid outlet at be provided with control
Liquid level detection device, the 1st reactive tank are provided with the normally closed type controlling switch whether runner processed circulates, the reservoir
Inlet at be provided with magneto hydrodynamic Micropump；
When being detected to sample to be detected, if the corresponding controlling switch open in usual of a-th of reactive tank it is controlled close and
Normally closed type controlling switch is controlled to open, then the detection liquid being pumped into by the inlet of the micro-fluidic chip flows through the described 1st successively
Individual reactive tank flows through the detect tank to the reservoir to a-th of reactive tank；If the liquid level detection device detection
The reservoir is full of to the detection liquid, then cue is exported, to point out stopping to be pumped into the detection liquid；When the magnetic current
Body power Micropump is controlled to be opened to promote during the detection liquid flowing in runner, and the detection liquid is respectively with inserting the described 1st
Electrode sensor of the individual reactive tank into a-th of reactive tank is hybridized；After the completion of hybridization, if the 1st reactive tank
Closed to the corresponding controlling switch open in usual of (a-1) individual reactive tank is controlled, the corresponding normally closed type control of a-th of reactive tank
Switch is controlled to close, then the 1st reactive tank forms the space of autonomous closure to a-th of reactive tank respectively；If described
Detect that the sample to be detected in liquid is normal, then any reactive tank pair in the 1st reactive tank into a-th of reactive tank
When the normally closed type controlling switch answered is controlled unlatching, the detection liquid after hybridization in the reactive tank flows into the detect tank to complete inspection
Survey process；Wherein, the detection liquid is mixed to get by the sample to be detected with the default buffer solution；A is more than 1
And the integer less than n.
The utility model is by using n reactive tank, runner, detect tank and reservoir including being arranged at Same Latitude
Micro-fluidic chip, due to being provided with control at the inlet of the 1st reactive tank and the first liquid outlet of preceding (n-1) individual reactive tank
Controlling stream is provided with the controlling switch open in usual whether runner processed circulates, and the second liquid outlet of preceding (n-1) individual reactive tank
The normally closed type controlling switch whether road circulates, therefore, can be by controlling opening for normally closed type controlling switch and controlling switch open in usual
Open or close to control the quantity of reactive tank needed to use, not Tongfang is carried out to same sample to be detected simultaneously so as to reach
The purpose of the detection in face, simplifies testing process, realize parting detection it is rapid, miniaturization and it is integrated.
Brief description of the drawings
Fig. 1 is a kind of vertical view for micro-fluidic chip suitable for multielectrode sensor that the utility model embodiment is provided
Figure；
Fig. 2 is the paraffin a kind of micro-fluidic chip suitable for multielectrode sensor that the utility model embodiment is provided
The folding schematic diagram of micro-valve；
Fig. 3 be the utility model embodiment provide a kind of micro-fluidic chip suitable for multielectrode sensor it is normally closed
The folding schematic diagram of formula magnetic force micro-valve.
Embodiment
In order that the purpose of this utility model, technical scheme and advantage are more clearly understood, below in conjunction with accompanying drawing and implementation
Example, the utility model is further elaborated.It should be appreciated that specific embodiment described herein is only to explain
The utility model, is not used to limit the utility model.
Fig. 1 is a kind of vertical view for micro-fluidic chip suitable for multielectrode sensor that the utility model embodiment is provided
Figure, for convenience of description, illustrate only the part related to the utility model embodiment, details are as follows：
As shown in figure 1, a kind of micro-fluidic chip suitable for multielectrode sensor, for electrode sensor and default
Buffer solution uses cooperatively to detect sample to be detected.The micro-fluidic chip includes being arranged at n reaction of Same Latitude
Groove X1~Xn (being illustrated in Fig. 1 so that micro-fluidic chip includes 7 reactive tanks as an example), runner 2, detect tank 3 and reservoir 4.
Wherein, n is the integer more than 1.
N reactive tank X1~Xn passes through stream since the 1st reactive tank X1 according to the first liquid outlet of previous reactive tank
The mode that road 2 connects the inlet of latter reactive tank is sequentially communicated to n-th of reactive tank Xn, the first of n-th of reactive tank Xn
Liquid outlet and preceding (n-1) individual reactive tank X1~X (n-1) the second liquid outlet are connected by runner 2 and the inlet of detect tank 3
Logical, the liquid outlet of detect tank 3 is connected by runner 2 with the inlet of reservoir 4 and the 1st reactive tank X1, the 1st reactive tank X1
Inlet be micro-fluidic chip inlet.
It is all provided with 1st reactive tank X1 inlet and preceding (n-1) individual reactive tank X1~X (n-1) the first liquid outlet
It is equipped with the controlling switch open in usual 5 whether control runner 2 circulates, preceding (n-1) individual reactive tank X1~X (n-1) the second liquid outlet
Place is provided with the normally closed type controlling switch 6 whether control runner 2 circulates, reservoir 4 and is provided with liquid level detection device (in figure
It is not shown), magneto hydrodynamic Micropump (not shown) is provided with the inlet of the 1st reactive tank.
When being detected to sample to be detected, closed if the corresponding controlling switches 5 open in usual of a-th of reactive tank Xa are controlled
Close, and the controlled unlatching of the corresponding normally closed type controlling switches 6 of a-th of reactive tank Xa, then it is pumped into by the inlet of micro-fluidic chip
It is corresponding that detection liquid flows through a-th of reactive tank Xa of the 1st reactive tank X1 to a-th reactive tank successively, cocurrent after testing groove 3 to storage
Liquid bath 4；If liquid level detection device detects detection, liquid is full of reservoir 4, cue is exported, to point out stopping to be pumped into detection
Liquid；When magneto hydrodynamic Micropump is controlled to be opened to promote the detection liquid in runner 2 to flow, detection liquid is respectively with inserting the 1st
Electrode sensor in reactive tank X1 to a-th reactive tank Xa is hybridized；After the completion of hybridization, if the 1st reactive tank X1 to
(a-1) the corresponding controlling switch 5 open in usual of individual reactive tank X (a-1) is controlled closes, the corresponding normally closed type controls of a-th of reactive tank Xa
System switch 6 is controlled to close, then the 1st reactive tank X1 to a-th reactive tank Xa forms the space of autonomous closure respectively；If detecting liquid
In sample to be detected it is normal, then the corresponding normally closed type of any reactive tank in the 1st reactive tank X1 to a-th reactive tank Xa
When controlling switch 6 is controlled unlatching, the detection liquid after hybridization in the reactive tank flows into detect tank 4 to complete detection process.
In the utility model embodiment, a is the integer more than 1 and less than n.
In the utility model embodiment, detection liquid is mixed to get by sample to be detected with default buffer solution.
In actual applications, default buffer solution can be that can carry out hybridization with detecting the buffer solution of two processes.Example
Such as, when carrying out many site primers to SNP, default buffer solution can include sodium perchlorate, SDS (sodium dodecyl
Sulfate, sodium salt, lauryl sodium sulfate), the reagent such as tween, it can carry out hybridization and detect two processes.
In actual applications, the micro-fluidic chip can be used for carrying out many site primers to gene, for example, available for SNP
(Single Nucleotide Polymorphisms, the polymorphism of mononucleotide) carries out many site primers, or to pathogen
Gene carries out many site primers etc., is determined, is not limited herein with specific reference to actual demand.When being detected, according to
The quantity of the gene loci detected is needed to determine the quantity of the reactive tank and electrode sensor needed to use, and then by normally opened
Formula controlling switch and normally closed type controlling switch control the quantity of reactive tank needed to use.
In the utility model embodiment, electrode sensor can be electrochemica biological sensor, and it is made with biomaterial
For sensing element, using electrode as conversion element, it is characterized to detect signal with potential or electric current.Due to electrochemical biosensor
Utensil has high selectivity, therefore, and it is quick, the direct preferable analysis tool for obtaining complex system composition information.
In the utility model embodiment, magneto hydrodynamic Micropump and reactive tank X1~Xn, runner 2, detect tank 3 and liquid storage
Groove 4 also is located at Same Latitude (same level).Magneto hydrodynamic Micropump is used to drive the detection liquid in runner to flow.Specifically
, magneto hydrodynamic Micropump can be arranged at the both sides of the runner at the inlet of the 1st reactive tank.In actual applications, magnetic
Fluid dynamic Micropump can be used using coil and magnetic element as the magneto hydrodynamic pump of core, can be by magneto hydrodynamic Micropump
The coil planted is arranged at the both sides of the runner at the inlet of the 1st reactive tank.When using magneto hydrodynamic Micropump, by coil
Power supply is connected to, makes have electric current to flow through in coil, and magnetic field is applied by magnetic element, so that magneto hydrodynamic Micropump produces drive
Power (Lorentz force), and then drive detection liquid to flow into a-th of reactive tank Xa successively from the 1st reactive tank X1.
As the embodiment of the utility model one, controlling switch 5 open in usual can be paraffin micro-valve.
Fig. 2 is the paraffin a kind of micro-fluidic chip suitable for multielectrode sensor that the utility model embodiment is provided
The folding schematic diagram of micro-valve.
As shown in Figure 2 a, in actual applications, paraffin micro-valve 5 can be arranged at the first of reactive tank X1~X (n-1) and go out liquid
On the outer wall of runner 2 at mouthful.Under normal circumstances, paraffin micro-valve is in opening, now, and corresponding runner 2 is in stream
Logical state.When needing to control corresponding runner 2 to close, heating paraffin wax can be led to, after paraffin melting and solidification, will can be flowed accordingly
Road 2 is closed, and now paraffin micro-valve is closed (as shown in Figure 2 b).
As the embodiment of the utility model one, normally closed type controlling switch 6 can be normally closed type magnetic force micro-valve.
Fig. 3 be the utility model embodiment provide a kind of micro-fluidic chip suitable for multielectrode sensor it is normally closed
The folding schematic diagram of formula magnetic force micro-valve.
In actual applications, normally closed type controlling switch 6 can be arranged at reactive tank X1~X (n-1) the second liquid outlet
Runner 2 outer wall on.
As shown in Figure 3 a, under normal circumstances, normally closed type magnetic force micro-valve is closed, now, at corresponding runner 2
In closed mode, when needing to control corresponding runner 2 to circulate, normally closed type magnetic force micro-valve can be controlled by applying magnetic field
Overarm arm is lifted, so that normally closed type magnetic force micro-valve is in opening (as shown in Figure 3 b), now, corresponding runner 2 just can be located
In circulation status.
As the embodiment of the utility model one, liquid level detection device can be pressure sensor.Pressure sensor can be set
It is placed in the top of reservoir.If pressure sensor detects detection, liquid is full of reservoir 4, illustrates that detection liquid is filled with entirely
Runner 2, now, pressure sensor output cue, to point out to stop feed liquor.
As the embodiment of the utility model one, detect tank 3 can include the first external circuitses work nest 31, negative control and examine
Survey groove 32, the external circuitses work nest 34 of positive control detect tank 33 and second.
Wherein, the inlet of the first external circuitses work nest 31 is the inlet of detect tank 3, the first external circuitses work nest
31 liquid outlet connects the inlet of negative control detect tank 32 by runner 2, and the liquid outlet of negative control detect tank 32 passes through
The inlet of the connection positive control of runner 2 detect tank 33, the liquid outlet of positive control detect tank 33 is connected outside second by runner 2
The inlet of circuit work nest 34 is connect, the liquid outlet of the second external circuitses work nest 34 is the liquid outlet of detect tank 3.
In the utility model implementation, the first external circuitses work nest 31 and the second external circuitses work nest 34 are for external
Power supply, forms current loop, and provide the potential stablized for reactive tank with work nest.
Specifically, the first external circuitses work nest 31 can include reference electrode groove 311 and auxiliary electrode groove 312.
The inlet of reference electrode groove 311 is the inlet of the first external circuitses work nest 31, and reference electrode groove 311 goes out
Liquid mouthful is connected by runner 2 with the inlet of auxiliary electrode groove 312, and the liquid outlet of auxiliary electrode groove 312 is the first external circuitses
The liquid outlet of work nest 31.
Second external circuitses work nest 34 can be auxiliary slot electrode.
In the utility model embodiment, negative control detect tank 32 and positive control detect tank 33 are used for detection liquid
In sample to be detected whether can normally use.In the case of the sample to be detected in detecting liquid is normal, it could carry out miscellaneous
Detection process after friendship.Specifically, the control group in negative control detect tank 32 and positive control detect tank 33 is with detecting in liquid
Detection sample react, if positive controls have result, negative control group is without result, you can card detection liquid in it is to be detected
Sample can be used normally.
Below by taking the SNP site for detecting DNA sample as an example, one kind that the utility model embodiment is provided is applied to more electric
The micro-fluidic chip of pole sensor is described further：
First, selection electrode sensor (i.e. detection chip), for example, select detectable SNP site rs1421085 (fat bases
Cause), rs1121980 (ob gene), rs1815739 (champion's gene) 3 detection chips, respectively insert the 1st reactive tank
In X1, the 2nd reactive tank X2 and the 3rd reactive tank X3.The corresponding controlling switches 5 open in usual of the 3rd reactive tank X3 are controlled to close,
To ensure that detection liquid may not flow into reactive tank below.Meanwhile, the corresponding normally closed type controlling switches of the 3rd reactive tank X3 of control
6 open, to ensure that detection liquid can flow to detect tank 3 and reservoir 4.
When being detected, DNA sample and the progress of default buffer solution are sufficiently mixed and obtain detecting liquid, detection liquid is led to
The inlet for crossing micro-fluidic chip is persistently pumped into, and detection liquid flows through the 1st reactive tank X1, the 2nd reactive tank X2, the 3rd reaction
After groove X3 and detect tank 3, reservoir 4 is flow to.Filled when the liquid level detection device in reservoir 4 detects reservoir 4 and is detected liquid
Man Shi, liquid level detection device output cue.When receiving cue, at the inlet for controlling the 1st reactive tank X1
Controlling switch open in usual 5 close, to stop feed liquor, now, the loop of three reactive tanks of series connection is formed in micro-fluidic chip.Open
Dynamic magneto hydrodynamic Micropump, flows the detection liquid in runner 2, to promote the crossover process for detecting liquid and 3 detection chips.It is miscellaneous
After the completion of friendship, the corresponding controlling switches 5 open in usual of the 1st reactive tank X1 and the 2nd reactive tank X2 of control are closed, and control the 3rd
The corresponding normally closed type controlling switch 6 of individual reactive tank X3 is closed, and now, the 1st reactive tank X1 to the 3rd reactive tank X3 is formed respectively
The space of autonomous closure.By negative control detect tank and positive control detect tank monitoring detection liquid in sample to be detected whether
Normally, if normally, being detected respectively to the detection liquid after the hybridization in the 1st reactive tank X1 to the 3rd reactive tank X3.Tool
Body, the 1st reactive tank X1 is switched on power, and its corresponding normally closed type controlling switch 6 is opened, make the 1st reactive tank X1 streams
Enter detect tank 3 to collect the 1st corresponding testing result of reactive tank X1；After collection is finished, the 1st reactive tank X1 of control is corresponding
Normally closed type controlling switch 6 is closed, and the 2nd reactive tank X2 is switched on power, and opens the 2nd corresponding normally closed type of reactive tank X2
Controlling switch 6, makes the 2nd reactive tank X2 flow into detect tank 3 to collect the 2nd corresponding testing result of reactive tank X2；Similarly, enter
The collection of the 3rd corresponding testing result of reactive tank X3 of row.
After testing result collection is finished, the 1st reactive tank X1 to the 3rd corresponding testing result of reactive tank X3 is entered respectively
Row analysis, you can obtain the genotypic results of 3 detected SNP sites.
In the utility model embodiment, crossover process is unified to be carried out, and shortens the reaction time, and detection process is realized
Automation, simplifies whole testing process.
The utility model embodiment is by using n reactive tank, runner, detect tank and storage including being arranged at Same Latitude
The micro-fluidic chip of liquid bath, due to being all provided with the inlet of the 1st reactive tank and the first liquid outlet of preceding (n-1) individual reactive tank
The controlling switch open in usual whether control runner circulates is equipped with, and is provided with the second liquid outlet of preceding (n-1) individual reactive tank
The normally closed type controlling switch whether control runner circulates, therefore, can be by controlling normally closed type controlling switch and control open in usual to open
What is closed is turned on and off controlling the quantity of reactive tank needed to use, and same sample to be detected is carried out simultaneously so as to reach
The purpose of the detection of different aspect, simplifies testing process, realize parting detection it is rapid, miniaturization and it is integrated.
Preferred embodiment of the present utility model is the foregoing is only, it is all at this not to limit the utility model
Any modifications, equivalent substitutions and improvements made within the spirit and principle of utility model etc., should be included in the utility model
Protection domain within.
Claims (7)
1. a kind of micro-fluidic chip suitable for multielectrode sensor, makes for coordinating with electrode sensor and default buffer solution
To be detected to sample to be detected, it is characterised in that the micro-fluidic chip includes being arranged at n reaction of Same Latitude
Groove, runner, detect tank and reservoir, n are the integer more than 1；
The n reactive tank since the 1st reactive tank, according to the first liquid outlet of previous reactive tank by flow passage after
The mode of the inlet of one reactive tank is sequentially communicated to n-th of reactive tank, the first liquid outlet of n-th of reactive tank and
Before second liquid outlet of (n-1) individual reactive tank connected by runner with the inlet of the detect tank, the detect tank goes out
Liquid mouthful is connected by runner with the inlet of reservoir and the 1st reactive tank, and the inlet of the 1st reactive tank is institute
State the inlet of micro-fluidic chip；
The inlet of 1st reactive tank and it is described before (n-1) individual reactive tank the first liquid outlet at be provided with control
The controlling switch open in usual whether runner circulates, it is described before (n-1) individual reactive tank the second liquid outlet at be provided with controlling stream
It is provided with liquid level detection device in the normally closed type controlling switch whether road circulates, the reservoir, the 1st reactive tank enters
Magneto hydrodynamic Micropump is provided with liquid mouthful；
When being detected to sample to be detected, closed and normally closed if the corresponding controlling switch open in usual of a-th of reactive tank is controlled
Formula controlling switch is controlled to open, then the detection liquid being pumped into by the inlet of the micro-fluidic chip flows through described 1st instead successively
Groove is answered to a-th of reactive tank, and flows through the detect tank to the reservoir；If the liquid level detection device detects institute
State detection liquid and be full of the reservoir, then cue is exported, to point out stopping to be pumped into the detection liquid；When the magnetic fluid is dynamic
Power Micropump is controlled to be opened to promote during the detection liquid flowing in runner, and the detection liquid is respectively with inserting described 1st instead
Electrode sensor of the groove into a-th of reactive tank is answered to be hybridized；After the completion of hybridization, if the 1st reactive tank is to
(a-1) the corresponding controlling switch open in usual of individual reactive tank is controlled closes, the corresponding normally closed type controlling switch of a-th of reactive tank
Controlled to close, then the 1st reactive tank forms the space of autonomous closure to a-th of reactive tank respectively；If the detection
Sample to be detected in liquid is normal, then any reactive tank in the 1st reactive tank into a-th of reactive tank is corresponding
When normally closed type controlling switch is controlled unlatching, the detection liquid after hybridization in the reactive tank flows into the detect tank to complete to detect
Journey；Wherein, the detection liquid is mixed to get by the sample to be detected with the default buffer solution；A is more than 1 and small
In n integer.
2. it is applied to the micro-fluidic chip of multielectrode sensor as claimed in claim 1, it is characterised in that the control open in usual
System switch is paraffin micro-valve.
3. it is applied to the micro-fluidic chip of multielectrode sensor as claimed in claim 1, it is characterised in that the normally closed type control
System switch is normally closed type magnetic force micro-valve.
4. it is applied to the micro-fluidic chip of multielectrode sensor as claimed in claim 1, it is characterised in that the level sensing
Device is pressure sensor；The pressure sensor is arranged at the top of the reservoir.
5. it is applied to the micro-fluidic chip of multielectrode sensor as claimed in claim 1, it is characterised in that the detect tank bag
Include the first external circuitses work nest, negative control detect tank, positive control detect tank and the second external circuitses work nest；
The inlet of the first external circuitses work nest is the inlet of the detect tank, the first external circuitses work nest
Liquid outlet by the inlet of negative control detect tank described in flow passage, the liquid outlet of the negative control detect tank passes through
The inlet of positive control detect tank described in flow passage, the liquid outlet of the positive control detect tank passes through described in flow passage
The inlet of second external circuitses work nest, the liquid outlet of the second external circuitses work nest goes out liquid for the detect tank
Mouthful.
6. it is applied to the micro-fluidic chip of multielectrode sensor as claimed in claim 5, it is characterised in that described first is external
Circuit work nest includes reference electrode groove and auxiliary electrode groove；
The inlet of the reference electrode groove is the inlet of the first external circuitses work nest, and the reference electrode groove goes out
Liquid mouthful is connected by runner with the inlet of the auxiliary electrode groove, and the liquid outlet of the auxiliary electrode groove is external for described first
The liquid outlet of circuit work nest.
7. it is applied to the micro-fluidic chip of multielectrode sensor as claimed in claim 5, it is characterised in that described second is external
Circuit work nest is auxiliary slot electrode.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720130759.0U CN206474190U (en) | 2017-02-10 | 2017-02-10 | A kind of micro-fluidic chip suitable for multielectrode sensor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720130759.0U CN206474190U (en) | 2017-02-10 | 2017-02-10 | A kind of micro-fluidic chip suitable for multielectrode sensor |
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| CN206474190U true CN206474190U (en) | 2017-09-08 |
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| CN201720130759.0U Expired - Fee Related CN206474190U (en) | 2017-02-10 | 2017-02-10 | A kind of micro-fluidic chip suitable for multielectrode sensor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108970653A (en) * | 2018-05-28 | 2018-12-11 | 辽宁工业大学 | A kind of sensor and preparation method based on micro-fluidic chip |
| CN110295107A (en) * | 2019-07-01 | 2019-10-01 | 贵州金玖生物技术有限公司 | A kind of multi-pass amount micro-fluidic chip for detection of nucleic acids |
-
2017
- 2017-02-10 CN CN201720130759.0U patent/CN206474190U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108970653A (en) * | 2018-05-28 | 2018-12-11 | 辽宁工业大学 | A kind of sensor and preparation method based on micro-fluidic chip |
| CN110295107A (en) * | 2019-07-01 | 2019-10-01 | 贵州金玖生物技术有限公司 | A kind of multi-pass amount micro-fluidic chip for detection of nucleic acids |
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