CN206420885U - A kind of mycoplasma pneumoniae IgM antibody detection kit - Google Patents
A kind of mycoplasma pneumoniae IgM antibody detection kit Download PDFInfo
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- CN206420885U CN206420885U CN201720077013.8U CN201720077013U CN206420885U CN 206420885 U CN206420885 U CN 206420885U CN 201720077013 U CN201720077013 U CN 201720077013U CN 206420885 U CN206420885 U CN 206420885U
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- upper plate
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- mycoplasma pneumoniae
- detection
- igm antibody
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Abstract
The utility model is related to Biological Detection technical field, it is specifically related to a kind of to obtain the mycoplasma pneumoniae IgM antibody detection kit of testing result rapidly, including box body and lid, provided with fixed support in box body, fixed support is provided with multiple grooves, provided with golden standard liquid bottle, Washing liquid bottle and detection plate in groove, detection plate includes the upper plate and lower plate being flexibly connected, lower plate is provided with adsorptive pads, upper plate is provided with watch window, upper plate inwall is provided with nitrocellulose filter with the watch window place of being adapted, and filter is provided with above watch window;Standard coloration band is additionally provided with the upper plate.It can be used for serum, the detection of blood plasma and whole blood sample using the utility model, improve detection sensitivity and be easy to staff to judge testing result.
Description
Technical field
The utility model is related to biology techniques detection field, more particularly to a kind of detection examination of mycoplasma pneumoniae IgM antibody
Agent box.
Background technology
Mycoplasma pneumoniae (M.Pneumonia) is the pathogen of mankind's Eaton agent pneumonia.Mainly through droplet infection, pneumonia branch
Substance is the pathogen for causing pneumonia more typical, and Children and teenager is multiple.Mycoplasma pneumoniae is different from common bacterium and disease
Poison, it is smaller than bacterium, but bigger than virus.It is the minimum microorganism that can be lived on one's own life.Eaton agent pneumonia whole year can fall ill, with
Autumn and winter is common.It, through air droplet transmission, causes respiratory tract infection by the mouth of patients during acute stage, nasal discharge.Its master that falls ill
It is relevant with indoor activity increase and close contact.Mycoplasma infection can also appear as pharyngitis, tracheobronchitis.
It is presently used for mycoplasma pneumoniae antigen, the kit of antibody test mainly has colloidal gold method, emulsion technique, enzyme-linked exempt from
Epidemic disease method, chemoluminescence method, fluorescent PCR method kit etc..In numerous methods colloidal gold method have it is convenient and swift, special it is sensitive,
Stability is strong, do not need the advantages of special installation and reagent, result judge directly perceived, therefore is commonly used to vast basic hospital, with
And among mass detection and large area census operations, with huge development potentiality and wide application prospect.
Collaurum is a kind of conventional labelling technique, is that the one of antigen-antibody is applied to using collaurum as tracer label thing
New immunolabelling technique is planted, there is that its is unique.In recent years widely used in various biological studies.Prior art
Collaurum percolation detection kit, general detection sample is serum, such as domestic patent《Cassette mycoplasma pneumoniae antibody is detected
Kit》(the patent No.:ZL200720021684.9) detection sample is serum, domestic patent《A kind of mycoplasma pneumoniae antibody examination
Paper slip》(the patent No.:ZL200720021685.3) detection sample is serum.Reviewer need to handle blood sample progress and obtain blood
It could be detected after clear, this make it that detection is relatively cumbersome.At present, mycoplasma pneumoniae IgM antibody is detected using colloidal gold technique
Kit producer it is numerous, therefore developing one kind can overcome colloidal gold diagnosis reagent sample process to require high, be easy to result
The kit of interpretation is very necessary.
Utility model content
Technical problem to be solved in the utility model is:In view of the shortcomings of the prior art there is provided one kind can be used for serum,
The detection of blood plasma and whole blood sample, improve detection sensitivity and be easy to staff to judge the mycoplasma pneumoniae IgM of testing result
Antibody assay kit.
In order to solve the above technical problems, the technical solution of the utility model is:
Provided with fixed support in a kind of mycoplasma pneumoniae IgM antibody detection kit, including box body and lid, the box body,
The fixed support is provided with multiple grooves, the groove provided with golden standard liquid bottle, Washing liquid bottle and detection plate, and the detection plate includes
The upper plate and lower plate being flexibly connected, the lower plate are provided with adsorptive pads, and the upper plate is provided with watch window, the upper plate
Block inwall is provided with nitrocellulose filter with the watch window place of being adapted, and filter is provided with above the watch window,
Standard coloration band is additionally provided with the upper plate.
As an improvement technical scheme, the upper plate be provided with stopper slot, the standard coloration band is arranged on spacing
In groove.
As an improvement technical scheme, the standard coloration with upper surface be provided with transparent material diaphragm.
As an improvement technical scheme, the filter is spoon shape, and the filter passes through bull stick and upper plate
Block is connected.
As an improvement technical scheme, the upper plate and lower plate is snap connection.
Employ after above-mentioned technical proposal, the beneficial effects of the utility model are:
(1) because upper plate increases standard coloration band, tester's sentence read result is facilitated, unified decision metrics make result more
Plus it is accurate;The accuracy of operating efficiency and result can be improved simultaneously, with certain practical value.
(2) the not permeable problem of sample is solved by filter, is more suitable for clinical examination;Add applicable sample type
(serum, blood plasma, whole blood), eliminates the link of sample process during use, time saving and energy saving;The reaction time is added, makes to resist
Antigen-antibody reaction is more abundant, improves the sensitivity of kit.
Brief description of the drawings
Fig. 1 is the structural representation of the utility model kit
Fig. 2 is the structural representation of detection plate in the utility model;
Fig. 3 is the structural representation of the upper plate of detection plate in Fig. 1;
Fig. 4 is the structural representation of the lower plate of detection plate in Fig. 1;
Wherein, 1- box bodys, 2- lids, 3- fixes plate under support, 4- grooves, the upper plates of 5-, 6-, 7- adsorptive pads, 8- observations
Window, 9- stopper slots, 10- nitrocellulose film layers, 11- standard coloration bands, 12- filters, 13- gold standard liquid bottles, 14- washings
Liquid bottle.
Embodiment
In order that the purpose of this utility model, technical scheme and advantage are more clearly understood, below in conjunction with accompanying drawing and implementation
Example, the utility model is further elaborated.It should be appreciated that specific embodiment described herein is only to explain
The utility model, is not used to limit the utility model.
A kind of mycoplasma pneumoniae IgM antibody detection kit, as shown in Figure 1, Figure 2, Fig. 3 and Fig. 4 it is jointly shown, including box body 1
With lid 2, box body 1 is interior to fix support 3 provided with sponge, and fixed support 3 is provided with groove 4, and groove 4 is interior provided with golden standard liquid bottle 13, cleaning solution
Bottle 14 and detection plate, detection plate include the upper plate 5 and lower plate 6 being flexibly connected, and lower plate 6 is provided with adsorptive pads 7, upper plate 5
Provided with watch window 8, the upper inwall of plate 5 is provided with nitrocellulose filter 10 with the place of being adapted of watch window 8, above watch window
Provided with filter 12, upper plate 5 is additionally provided with standard coloration band 11.
As an improvement, upper plate 5 is provided with stopper slot 9, and standard coloration band 5 is sticked in stopper slot 9 by double faced adhesive tape.
As an improvement, the upper surface of standard coloration band 11 is provided with the diaphragm of transparent material.
As an improvement, filter 12 is spoon shape, and filter 12 is connected by bull stick with upper plate 5.Filtering
The size and shape of device is adapted to observation window, and filter is made up of the framework of filter membrane and plastic material.
As a preferred embodiment, upper plate 5 is provided with card column, lower plate 6 is provided with the neck being adapted with card column.
The application method of kit comprises the following steps:
1. take out kit, equilibrium at room temperature 20~30 minutes;
2. cleaning solution 2 is added dropwise to drip in reacting hole, treat that liquid moistens film completely;
3. adding the μ L of test serum 50 (adding 2 drops if with sample suction pipe) in reacting hole, treat that liquid is fully sucked;
4. golden standard liquid 3 is added dropwise to drip in reacting hole, treat that liquid is fully sucked;
5. cleaning solution 3 is added dropwise to drip in reacting hole, treat that liquid is fully sucked after observing result in 3 minutes.
The explanation of assay:
1. it is negative --- Quality Control point C displays are red, and test point T location occurs without red point;
2. it is positive --- Quality Control point C displays are red, and test point T location has red point to occur;
3. it is invalid --- Quality Control point C, which does not develop the color, shows operational error or reagent failure.
Testing result is compared with standard coloration band:
Test point is compared with standard coloration band color, is judged positive strong and weak, is instructed clinical practice.
In summary, the utility model facilitates tester's sentence read result by increasing standard coloration band, can improve work effect
Rate, unified decision metrics, improves the accuracy of result, with certain practical value.Sample is solved by filter not permeable
Problem, is more suitable for clinical examination;Applicable sample type (serum, blood plasma, whole blood) is added, sample during use is eliminated
The link of processing, it is time saving and energy saving;The reaction time is added, makes antigen-antibody reaction more abundant, the sensitivity of kit is improved.
This patent is not limited to above-mentioned specific embodiment, one of ordinary skill in the art from above-mentioned design,
Without performing creative labour, made a variety of conversion are all fallen within the protection domain of this patent.
Claims (5)
1. a kind of mycoplasma pneumoniae IgM antibody detection kit, including box body and lid, it is characterised in that:Set in the box body
There is fixed support, the fixed support is provided with multiple grooves, the groove provided with golden standard liquid bottle, Washing liquid bottle and detection plate, described
Detection plate includes the upper plate and lower plate being flexibly connected, and the lower plate is provided with adsorptive pads, and the upper plate is provided with observation window
Mouthful, the upper plate inwall is provided with nitrocellulose filter with the watch window place of being adapted, and is set above the watch window
There is filter;Standard coloration band is additionally provided with the upper plate.
2. a kind of mycoplasma pneumoniae IgM antibody detection kit according to claim 1, it is characterised in that:The upper plate
Block is provided with stopper slot, and the standard coloration band is arranged in stopper slot.
3. a kind of mycoplasma pneumoniae IgM antibody detection kit according to claim 1, it is characterised in that:The standard
Developed band upper surface is provided with the diaphragm of transparent material.
4. a kind of mycoplasma pneumoniae IgM antibody detection kit according to claim 1, it is characterised in that:The filtering
Device is spoon shape, and the filter is connected by bull stick with upper plate.
5. a kind of mycoplasma pneumoniae IgM antibody detection kit according to claim 1, it is characterised in that:The upper plate
Block and lower plate are to snap connection.
Priority Applications (1)
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CN201720077013.8U CN206420885U (en) | 2017-01-21 | 2017-01-21 | A kind of mycoplasma pneumoniae IgM antibody detection kit |
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CN201720077013.8U CN206420885U (en) | 2017-01-21 | 2017-01-21 | A kind of mycoplasma pneumoniae IgM antibody detection kit |
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CN206420885U true CN206420885U (en) | 2017-08-18 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109799338A (en) * | 2019-01-14 | 2019-05-24 | 湖南达道生物工程有限公司 | A kind of test paper and its application suitable for peripheral blood immunochromatography quantitative detection |
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2017
- 2017-01-21 CN CN201720077013.8U patent/CN206420885U/en active Active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109799338A (en) * | 2019-01-14 | 2019-05-24 | 湖南达道生物工程有限公司 | A kind of test paper and its application suitable for peripheral blood immunochromatography quantitative detection |
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CP03 | Change of name, title or address |
Address after: No.699, Yuehe Road, Weifang Economic Development Zone, Weifang City, Shandong Province Patentee after: Shandong Kanghua Biomedical Technology Co., Ltd Address before: 261023 Shandong city of Weifang Province River Road Economic Development Zone No. 699 Patentee before: WEIFANG KANGHUA BIOTECH Co.,Ltd. |