CN206408213U - Magnetic bead transfer device and the nucleic acid extraction instrument for including the magnetic bead transfer device - Google Patents
Magnetic bead transfer device and the nucleic acid extraction instrument for including the magnetic bead transfer device Download PDFInfo
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- CN206408213U CN206408213U CN201720041215.7U CN201720041215U CN206408213U CN 206408213 U CN206408213 U CN 206408213U CN 201720041215 U CN201720041215 U CN 201720041215U CN 206408213 U CN206408213 U CN 206408213U
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- substrate
- bar magnet
- magnetic bead
- transfer device
- magnetosheath
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Abstract
The utility model is related to a kind of magnetic bead transfer device and includes the nucleic acid extraction instrument of the magnetic bead transfer device, including substrate, and the substrate is provided with substrate actuator；Magnetosheath frame, is installed on the substrate, and the magnetosheath frame is provided with hollow and one end open magnetosheath；Bar magnet mechanism, the bar magnet mechanism is provided with the magnetic bar magnet of tool, the bar magnet mechanism is installed on the substrate and relatively described magnetosheath frame can moved back and forth under the driving of the substrate actuator, is stretched into driving the relatively described magnetosheath frame of the bar magnet to move back and forth or away from the magnetosheath；Travel mechanism, with moveable transfer arm, the transfer arm and the substrate connection are to drive the substrate to move.The magnetic bead transfer device can save the operation of manually absorption migration magnetic bead, and stability is good, and magnetic bead transfer efficiency is greatly improved, thus significantly improve nucleic acid extraction efficiency and quality, and the apparatus structure is simple, and cost is low, has a good application prospect.
Description
Technical field
The utility model is related to biotechnological device field, more particularly to a kind of magnetic bead transfer device and including the magnetic bead
The nucleic acid extraction instrument of transfer device.
Background technology
In recent years, with the high speed development of molecular biology, large quantities of molecular biosciences based on nucleic acid have been emerged
Experiment, such as sequencing of two generations, quantitative fluorescent PCR, genetic chip, making nucleic acid molecular hybridization are learned, and DNA integrality, RNA degrade
Degree, the purity of nucleic acid and concentration etc. all produce more or less influence to molecular biology experiment.In addition precisely medical treatment is included
Inside, the especially emergency treatment of the medical applications based on nucleic acid is just being obtained more and more using (such as cause of disease quick diagnosis technology)
Pay attention to and apply, strong influence traditional medical diagnostic method, improve quick diagnosis level.
Method for extracting nucleic acid main at present is paramagnetic particle method, foundation and pellosil centrifugal column identical principle, with nanometer
Technology the surface of superparamagnetic nano particle is improved and surface modification after, be prepared into superparamagnetism silica nano magnetic
Pearl, the magnetic bead can specifically be recognized with nucleic acid molecules and efficiently combined on micro interface.Utilize silica nanoparticle
Superparamagnetism, can be from blood, animal tissue, food, cause of disease in the presence of guanidine hydrochloride, guanidinium isothiocyanate etc. and externally-applied magnetic field
Nucleic acid is isolated in microorganism equal samples.
Paramagnetic particle method nucleic acid extraction can be generally divided into cracking, with reference to, washing and elute, however, by manually carrying out
Magnetic bead transfer operation is needed to expend long time, influences the efficiency of nucleic acid extraction, and nucleic acid is easily degraded, and extraction time is long
The quality of extracted nucleic acid can be influenceed.
Utility model content
Based on this, it is necessary to long for the above-mentioned manually progress magnetic bead transfer operation consuming time, nucleic acid extraction efficiency
Low the problem of, there is provided a kind of simple efficient magnetic bead transfer device.
Specific technical scheme is as follows：
A kind of magnetic bead transfer device, including：
Substrate, the substrate is provided with substrate actuator；
Magnetosheath frame, is installed on the substrate, and the magnetosheath frame is provided with hollow and one end open magnetosheath；
Bar magnet mechanism, the bar magnet mechanism is installed on the substrate provided with having magnetic bar magnet, the bar magnet mechanism
And relatively described magnetosheath frame can be moved back and forth under the driving of the substrate actuator, to drive the relatively described magnetosheath of the bar magnet
Frame is moved back and forth and stretched into or the remote magnetosheath；
Travel mechanism, with moveable transfer arm, the transfer arm and the substrate connection are to drive the substrate to move
It is dynamic.
Above-mentioned magnetic bead transfer device in use, first can place a sample into reacting hole, by lysate, with reference to liquid destruction carefully
Born of the same parents' structure, makes the abundant separate out of nucleic acid, is combined with the magnetic bead in solution, then drives substrate by the transfer arm of travel mechanism
Movement makes magnetosheath stretch into reacting hole, then drives bar magnet mechanism kinematic bar magnet is stretched into magnetosheath by substrate actuator, magnetic bead
And the nucleic acid combined with magnetic bead is then adsorbed on magnetosheath.Then moving substrate makes magnetosheath extend into the reacting hole for washing,
Bar magnet mechanism kinematic is driven to make bar magnet away from magnetosheath by substrate actuator, dynamic respons container makes magnetic bead and nucleic acid depart from magnetosheath
It is dispersed in cleaning solution, and residual impurity is removed by cleaning solution washing.Then substrate actuator drives bar magnet mechanism to transport again
Dynamic bar magnet is stretched into magnetosheath absorption magnetic bead and nucleic acid, then moving substrate stretches into magnetosheath in the reacting hole for elution, passes through
Nucleic acid of the eluent Dissociative on magnetic bead, last moving substrate is moved away the magnetic bead that finishes is eluted, high concentration and high-purity
The nucleic acid of degree is then stayed in reacting hole.By the magnetic bead transfer device, the operation of manually absorption migration magnetic bead is eliminated, surely
Qualitative good, magnetic bead transfer efficiency is greatly improved, thus significantly improves nucleic acid extraction efficiency and quality, and apparatus structure letter
Single, cost is low, has a good application prospect.
In one of the embodiments, the substrate is provided with guide rail slide block, and the guide rail slide block is described including being fixed on
The guide rail of substrate and the sliding block that can be slided along the guide rail, the bar magnet mechanism are connected to be installed on the base with the sliding block
Plate, the substrate actuator is connected to drive the bar magnet mechanism to move back and forth along the guide rail with the bar magnet mechanism.
In one of the embodiments, the bar magnet mechanism includes connecting plate and bar magnet frame, described connecting plate one end and institute
Sliding block connection is stated, the other end away from the sliding block is connected with the bar magnet frame, and the bar magnet is located at the bar magnet frame.
In one of the embodiments, the substrate actuator is provided with substrate drive shaft, and the substrate actuator passes through
The substrate drive shaft is threadedly coupled with the connecting plate, and the substrate actuator can drive the substrate drive shaft rotation with band
The connecting plate is moved to move back and forth along the guide rail.
In one of the embodiments, the bar magnet frame is provided with magnetic part mounting groove, the magnetic part mounting groove and pacified
Equipped with magnetic part, the bar magnet is contacted with the magnetic part.
In one of the embodiments, the bar magnet mechanism be provided with bar magnet location sensitive plate, the substrate be provided with
The first position induction pieces and second place induction pieces of the substrate actuator communication connection, the bar magnet mechanism moves back and forth pre-
If apart from when, the bar magnet location sensitive plate is corresponding with the first position induction pieces or the second place induction pieces position,
So that the first position induction pieces or the second place induction pieces control the substrate actuator to stop, so as to limit described
The reciprocating distance of bar magnet mechanism.
In one of the embodiments, the magnetic part mounting groove two ends are provided with magnetic part fixed plate, and the magnetic part is consolidated
Fixed board abuts the magnetic part being fixed in the magnetic part mounting groove with the magnetic part.
In one of the embodiments, the quantity of the bar magnet is multiple, a pair of multiple magnetosheaths and the bar magnet 1
Should.
In one of the embodiments, a diameter of 2mm-10mm of the magnetosheath.
Another object of the present utility model is to provide a kind of nucleic acid extraction instrument, including above-mentioned magnetic bead transfer device.
Brief description of the drawings
Fig. 1 for an embodiment magnetic bead transfer device bar magnet away from magnetosheath when structural representation；
Fig. 2 stretches into structural representation during magnetosheath for the magnetic bead transfer device bar magnet shown in Fig. 1.
Embodiment
For the ease of understanding the utility model, the utility model is more fully retouched below with reference to relevant drawings
State.Preferred embodiment of the present utility model is given in accompanying drawing.But, the utility model can come real in many different forms
It is existing, however it is not limited to embodiment described herein.On the contrary, the purpose for providing these embodiments is made to public affairs of the present utility model
The understanding for opening content is more thorough comprehensive.
It should be noted that when element is referred to as " being installed on " another element, it can be directly on another element
Or can also have element placed in the middle.When an element is considered as " connection " another element, it can be direct connection
To another element or it may be simultaneously present centering elements.When an element is considered as " connection " another element, it can
To be directly to another element or may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article is led with belonging to technology of the present utility model
The implication that the technical staff in domain is generally understood that is identical.It is herein to be in term used in the description of the present utility model
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein "and/or" includes
The arbitrary and all combination of one or more related Listed Items.
As depicted in figs. 1 and 2, a kind of magnetic bead transfer device 300 of the preferred embodiment of the utility model one, including substrate
310th, magnetosheath frame 320, bar magnet mechanism 330 and travel mechanism's (not shown).
Substrate 310 is provided with substrate actuator 311, and magnetosheath frame 320 is installed on substrate 310, and magnetosheath frame 320 is provided with hollow
And the magnetosheath 321 of one end open.Bar magnet mechanism 330 is installed on substrate provided with having magnetic bar magnet 331, bar magnet mechanism 330
310 and relative magnetosheath frame 320 it can be moved back and forth under the driving of substrate actuator 311, to drive bar magnet 331 with respect to magnetosheath frame
320 move back and forth, so that bar magnet 331 is stretched into or away from magnetosheath 321.Travel mechanism has moveable transfer arm 341, mobile
Arm 341 is connected to drive substrate 310 to move with substrate 310.Specifically in the present embodiment, transfer arm 341 can drive the edge of substrate 310
Axial direction or the radial direction movement of bar magnet 331, it will be understood that move mode not limited to this, can be according to the transfer need of magnetic bead
It is adjusted.
The magnetic bead transfer device 300 of the present embodiment leads in use, can first place a sample into the reacting hole of reaction plate 200
Cross lysate, destroy eucaryotic cell structure with reference to liquid, make the abundant separate out of nucleic acid, combined with the magnetic bead in solution, then by moving
The transfer arm 341 of motivation structure drives the movement of substrate 310 magnetosheath 321 is stretched into reacting hole, then is driven by substrate actuator 311
The motion of bar magnet mechanism 330 makes bar magnet 331 stretch into magnetosheath 321, and magnetic bead and the nucleic acid combined with magnetic bead are then adsorbed in magnetosheath 321
On.Then moving substrate 310 makes magnetosheath 321 extend into the reacting hole for washing, and bar magnet is driven by substrate actuator 311
The motion of mechanism 330 makes bar magnet 331 away from magnetosheath 321, and dynamic respons plate 200 makes magnetic bead and nucleic acid disengaging magnetosheath 321 be dispersed in and wash
Wash in liquid, and residual impurity is removed by cleaning solution washing.Then substrate actuator 311 drives bar magnet mechanism 330 to move again
Bar magnet 331 is set to stretch into the absorption magnetic bead of magnetosheath 321 and nucleic acid, then moving substrate 310 stretches into magnetosheath 321 reaction for elution
Kong Zhong, by nucleic acid of the eluent Dissociative on magnetic bead, last moving substrate 310 moves away the magnetic bead that elution is finished,
The nucleic acid of high concentration and high-purity is then stayed in reacting hole.By the magnetic bead transfer device 300, eliminate manually absorption and turn
The operation of magnetic bead is moved, stability is good, magnetic bead transfer efficiency is greatly improved, thus significantly improves nucleic acid extraction efficiency and quality,
And the apparatus structure is simple, cost is low, has a good application prospect.
Specifically, substrate 310 is provided with guide rail slide block 312, and guide rail slide block 312 includes the guide rail for being fixed on substrate 310
3121 and the sliding block 3122 that can be slided along guide rail 3121, bar magnet mechanism 330 is connected to be installed on substrate 310, base with sliding block 3122
Plate actuator 311 is connected to drive bar magnet mechanism 330 to move back and forth along guide rail 3121 with bar magnet mechanism 330.In the present embodiment,
Substrate actuator 311 is linear stepping motor, is fixed on by substrate actuator support frame 3111 on substrate 310.
Specifically, bar magnet mechanism 330 includes connecting plate 332 and bar magnet frame 333, and the one end of connecting plate 332 connects with sliding block 3122
Connect, the other end away from sliding block 3122 is connected with bar magnet frame 333, bar magnet 331 is located at bar magnet frame 333.
Further, substrate actuator 311 is provided with substrate drive shaft 3112, and substrate actuator 311 is driven by substrate
Axle 3112 is threadedly coupled with connecting plate 332, substrate actuator 311 the substrate drive shaft 311 can be driven to rotate in different directions with
Connecting plate 332 is driven to be moved back and forth along guide rail 3121.
Preferably, bar magnet frame 333 is provided with magnetic part mounting groove 3331, magnetic part mounting groove 3331 and is provided with magnetic part
3332, bar magnet 331 is contacted with magnetic part 3332, so as to strengthen the magnetic of bar magnet 331 by magnetic part 3332, makes magnetic bead energy of adsorption
Power is more preferable.
Preferably, bar magnet mechanism 330 is provided with bar magnet location sensitive plate 334, and substrate 310 is provided with and substrate actuator
The first position induction pieces 313 and second place induction pieces 314 of 311 communication connections, bar magnet mechanism 330 move back and forth pre-determined distance
When, bar magnet location sensitive plate 334 is corresponding with first position induction pieces 313 or the position of second place induction pieces 314, so that first
Put induction pieces 313 or the control base board actuator 311 of second place induction pieces 314 stops, so as to limit the back and forth fortune of bar magnet mechanism 330
Dynamic distance, makes the relative distance of bar magnet 331 and magnetosheath 321 control more stable and accurate.In the present embodiment, substrate 310
On be fixed with circuit board 315, first position induction pieces 313 and second place induction pieces 314 are optocoupler, are installed on circuit board 315,
With sensing groove, bar magnet mechanism 330, which is moved back and forth, can be such that bar magnet location sensitive plate 334 stretches into corresponding sensing groove, so that the
One location sensitive part 313 or the control base board actuator 311 of second place induction pieces 314 stop, and prevent bar magnet mechanism 330 from continuing original
The motion in direction.
Preferably, the two ends of magnetic part mounting groove 3331 are provided with magnetic part fixed plate 3333, magnetic part fixed plate 3333 and magnetic
Property part 3332 abuts magnetic part 3332 being fixed in magnetic part mounting groove 3331, so as to keep the stabilization of magnetic part 3332.
In the present embodiment, the quantity of bar magnet 331 is 12, and 12 magnetosheaths 321 are corresponded with bar magnet 331, and bar magnet 331 is in
Arranged in a straight line, the reacting hole with 96 orifice plates is corresponding.It is appreciated that the quantity and arrangement mode of bar magnet 331 can be as needed
Adjustment, to adapt to different reaction vessel and demand.
Further, a diameter of 2mm-10mm of magnetosheath 321, the sample volume for extraction is minimum up to 20uL, energy
Enough save sample and cost.
The magnetic bead transfer device 300 of the present embodiment can save the operation of manually absorption migration magnetic bead, and stability is good, magnetic
Pearl transfer efficiency is greatly improved, thus significantly improves nucleic acid extraction efficiency and quality, and the apparatus structure is simple, and cost is low,
Have a good application prospect.
As shown in figure 1, the nucleic acid extraction instrument (not shown) of the preferred embodiment of the utility model one, including above-mentioned magnetic bead turn
Moving device 300.Nucleic acid extraction instrument of the present utility model eliminates manually absorption migration magnetic by magnetic bead transfer device 300
The operation of pearl, stability is good, and magnetic bead transfer efficiency is greatly improved, thus significantly improves nucleic acid extraction efficiency and quality, and should
Apparatus structure is simple, and cost is low, has a good application prospect.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope of this specification record is all considered to be.
Embodiment described above only expresses several embodiments of the present utility model, and it describes more specific and detailed,
But therefore it can not be interpreted as the limitation to utility model patent scope.It should be pointed out that for the common skill of this area
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
Protection domain of the present utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (10)
1. a kind of magnetic bead transfer device, it is characterised in that including：
Substrate, the substrate is provided with substrate actuator；
Magnetosheath frame, is installed on the substrate, and the magnetosheath frame is provided with hollow and one end open magnetosheath；
Bar magnet mechanism, the bar magnet mechanism is installed on the substrate and can provided with having magnetic bar magnet, the bar magnet mechanism
Relatively described magnetosheath frame is moved back and forth under the driving of the substrate actuator, to drive the relatively described magnetosheath frame of the bar magnet past
Move and stretch into or the remote magnetosheath again；
Travel mechanism, with moveable transfer arm, the transfer arm and the substrate connection are to drive the substrate to move.
2. magnetic bead transfer device according to claim 1, it is characterised in that the substrate is provided with guide rail slide block, described
Guide rail slide block includes the guide rail for being fixed on the substrate and the sliding block that can be slided along the guide rail, the bar magnet mechanism and the cunning
Block is connected to be installed on the substrate, and the substrate actuator is connected to drive the bar magnet mechanism along institute with the bar magnet mechanism
State guide rail reciprocating motion.
3. magnetic bead transfer device according to claim 2, it is characterised in that the bar magnet mechanism includes connecting plate and bar magnet
Frame, described connecting plate one end is connected with the sliding block, and the other end away from the sliding block is connected with the bar magnet frame, the bar magnet
Located at the bar magnet frame.
4. magnetic bead transfer device according to claim 3, it is characterised in that the substrate actuator drives provided with substrate
Axle, the substrate actuator is threadedly coupled by the substrate drive shaft with the connecting plate, and the substrate actuator can drive
The substrate drive shaft rotates to drive the connecting plate to move back and forth along the guide rail.
5. magnetic bead transfer device according to claim 3, it is characterised in that the bar magnet frame is installed provided with magnetic part
Magnetic part is installed in groove, the magnetic part mounting groove, the bar magnet is contacted with the magnetic part.
6. magnetic bead transfer device according to claim 1, it is characterised in that the bar magnet mechanism is felt provided with bar magnet position
Plate is answered, the substrate is provided with the first position induction pieces and second place induction pieces communicated to connect with the substrate actuator,
When the bar magnet mechanism moves back and forth pre-determined distance, the bar magnet location sensitive plate and the first position induction pieces or described the
Two location sensitive part positions correspondence, so that the first position induction pieces or the second place induction pieces control the substrate to drive
Moving part stops, so as to limit the reciprocating distance of bar magnet mechanism.
7. magnetic bead transfer device according to claim 5, it is characterised in that the magnetic part mounting groove two ends are provided with magnetic
Part fixed plate, the magnetic part fixed plate abuts the magnetic part being fixed on the magnetic part mounting groove with the magnetic part
It is interior.
8. magnetic bead transfer device according to claim 1, it is characterised in that the quantity of the bar magnet is multiple, Duo Gesuo
Magnetosheath is stated to correspond with the bar magnet.
9. the magnetic bead transfer device according to claim any one of 1-8, it is characterised in that a diameter of 2mm- of the magnetosheath
10mm。
10. a kind of nucleic acid extraction instrument, it is characterised in that including the magnetic bead transfer device described in claim any one of 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720041215.7U CN206408213U (en) | 2017-01-13 | 2017-01-13 | Magnetic bead transfer device and the nucleic acid extraction instrument for including the magnetic bead transfer device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720041215.7U CN206408213U (en) | 2017-01-13 | 2017-01-13 | Magnetic bead transfer device and the nucleic acid extraction instrument for including the magnetic bead transfer device |
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| Publication Number | Publication Date |
|---|---|
| CN206408213U true CN206408213U (en) | 2017-08-15 |
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| CN201720041215.7U Active CN206408213U (en) | 2017-01-13 | 2017-01-13 | Magnetic bead transfer device and the nucleic acid extraction instrument for including the magnetic bead transfer device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112226362A (en) * | 2020-12-11 | 2021-01-15 | 博奥生物集团有限公司 | Nucleic acid analysis cartridge and nucleic acid analysis apparatus |
-
2017
- 2017-01-13 CN CN201720041215.7U patent/CN206408213U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112226362A (en) * | 2020-12-11 | 2021-01-15 | 博奥生物集团有限公司 | Nucleic acid analysis cartridge and nucleic acid analysis apparatus |
| CN112226362B (en) * | 2020-12-11 | 2021-03-12 | 博奥生物集团有限公司 | Nucleic acid analysis cartridge and nucleic acid analysis apparatus |
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