CN206385166U - A kind of DNA cloning device - Google Patents
A kind of DNA cloning device Download PDFInfo
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- CN206385166U CN206385166U CN201720063883.XU CN201720063883U CN206385166U CN 206385166 U CN206385166 U CN 206385166U CN 201720063883 U CN201720063883 U CN 201720063883U CN 206385166 U CN206385166 U CN 206385166U
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Abstract
A kind of DNA cloning device, including microcontroller, sample introduction tank, the micro- injector connected with sample introduction tank and the reactive tank being connected with micro- injector by capillary, reactive tank is double cylinder, space between internal layer cylinder and outer layer cylinder is divided into three thermostatic chambers, capillary equably spiral wrap on internal layer cylinder, the upper, middle and lower of internal layer cylinder sets three pieces of micro- heating chips, what micro- heating chip was connected by pad and with pad is uniformly distributed three heating electrodes, electrode is heated to stretch out in internal layer cylinder to three thermostatic chambers, leave passage in the centre of heating electrode, temperature sensor is provided with passage, microcontroller is connected by circuit with three pieces of micro- heating chips.Reaction solution is at the uniform velocity flowed in capillary, and heating is continuously circulated by thermostatic chamber, and layering sets heating chip, stable temperature control, it is simple in construction, it is easy to operate, amplification, and the amplification that can maximize can be minimized, amplification efficiency is improved, and cost is low, the lifting cost performance of PCR amplification instrument.
Description
Technical field
The utility model is related to biological technical field, more particularly to a kind of DNA cloning device.
Background technology
PCR is isothermal DNA amplification, with special, sensitive, yield it is high, quick, easy, repeat
Property is good, the features such as easily automate, can in a test tube by the target gene to be studied or a certain DNA fragmentation in a few hours
100,000 or even million times are expanded to, PCR amplifications several hours can just complete, PCR reacts 3 step, one circulation, each circulation is passed through
3 constant temperature stages are spent, high-temperature denatured, process annealing, the extension of middle temperature again expand DNA with index by the circulation of 30 or so,
In DNA cloning instrument alternating temperature heater, silicon heating is most common one kind, and with easy realization automation, speed is fast, directly heat
Cooling, the characteristics of effect is good, the medium used is metal, though heat conduction it is good with reaction tube be difficult accomplish it is seamless contact, and
And bottom-heated is typically disposed in, low pressure is larger, the possibility for having spilling.
The content of the invention
The purpose of this utility model is to provide a kind of stable temperature control, and heating cooling rate is fast, cheap and avoid overflowing
The DNA cloning device of leakage problem.
Technical solution adopted in the utility model：
A kind of DNA cloning device, including microcontroller, sample introduction tank, micro- injector for being connected with sample introduction tank and with micro- sample introduction
The reactive tank that device is connected by capillary, reactive tank is double cylinder, and the space between internal layer cylinder and outer layer cylinder is divided into three
Individual thermostatic chamber, respectively high-temperature denatured thermostatic chamber, process annealing thermostatic chamber and middle temperature extension thermostatic chamber, capillary equably spiral
Formula is wrapped on internal layer cylinder, and the upper, middle and lower of internal layer cylinder sets three pieces of micro- heating chips, micro- heating chip by pad and with
Being uniformly distributed in three heating electrodes, heating electrode stretching internal layer cylinder to three thermostatic chambers for pad connection, heats electrode
Centre is left and temperature sensor is provided with passage, passage, and microcontroller is connected by circuit with three pieces of micro- heating chips.
The material of the nexine cylinder and three thermostatic chambers is heat-barrier material.
Described capillary uses seal washer when passing through different constant temperature zones.
Described capillary finally extends on internal layer cylinder after spiral winding 30~45 is enclosed at the top of reactive tank.
The beneficial effects of the utility model：Reaction solution at the uniform velocity flows in capillary, is continuously circulated by thermostatic chamber
Heating, layering sets heating chip, and stable temperature control is simple in construction, easy to operate, can minimize amplification, and the expansion that can maximize
Increase, improve amplification efficiency, and cost is low.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Wherein：1- sample introduction tanks；The micro- injectors of 2-；3- capillaries；4- temperature sensors；5- pads；6- heats electrode；7- is micro-
Controller；8- circuits；The high-temperature denatured thermostatic chambers of 9-；10- process annealing thermostatic chambers；The middle temperature extension thermostatic chambers of 11-；12- internal layer posts
Face.
Embodiment
A kind of DNA cloning device, including microcontroller 7, sample introduction tank 1, the micro- injector 2 connected with sample introduction tank 1 and with it is micro-
The reactive tank that injector 2 is connected by capillary 3, reactive tank is double cylinder, the sky between internal layer cylinder 12 and outer layer cylinder
Three thermostatic chambers are partitioned into, respectively high-temperature denatured thermostatic chamber 9, process annealing thermostatic chamber 10 and middle temperature extension thermostatic chamber 11, hair
Equably spiral wrap is on internal layer cylinder 12 for tubule 3, and the upper, middle and lower of internal layer cylinder sets three pieces of micro- heating chips, micro- to add
What hot chip was connected by pad 5 and with pad 5 be uniformly distributed, and three heating electrodes 6 are constituted, and heating electrode 6 stretches out internal layer cylinder 12
To three thermostatic chambers, the centre of heating electrode 6, which is left, is provided with temperature sensor 4 in passage, passage, microcontroller 7 passes through
Circuit 8 is connected with three pieces of micro- heating chips.
The material of the nexine cylinder 12 and three thermostatic chambers is heat-barrier material.
Described capillary 3 uses seal washer when passing through different constant temperature zones.
Described capillary 3 finally extends after spiral winding 30~45 is enclosed on internal layer cylinder 12 from the top of reactive tank
Go out.
Sample is added from sample introduction tank 1, the spiral entered under the promotion of micro- injector 2 of adjustable sample introduction speed in reactive tank
In capillary 3, now the temperature of three thermostatic chambers is controlled three heating electrodes of micro- heating chip by microcontroller 7 in reactive tank
6 are warming up to fast and accurately reaction heating in the temperature needed for each thermostatic chamber, the passage of the insertion heating electrode of temperature sensor 4
The temperature of electrode 6, is accurately controlled constant temperature room temperature, and sample circulates shuttle, 30 along capillary 3 in differential responses phase temperature
The capillary of reactive tank is left after~45 circulations, now reaction terminates, and then refrigerates.
The volume of DNA cloning three thermostatic chambers of device is formulated according to sample size, the small-sized dress of experiment can be both designed to
Put, can also be prepared into for industrialized large-scale DNA cloning instrument.
One embodiment of the present utility model is described in detail above, but the content is only of the present utility model
Preferred embodiment, it is impossible to be considered as being used to limit practical range of the present utility model.It is all to be made according to present utility model application scope
Equivalent change with improve etc., all should still belong within patent covering scope of the present utility model.
Claims (4)
1. a kind of DNA cloning device, it is characterised in that connected including microcontroller (7), sample introduction tank (1), with sample introduction tank (1)
Micro- injector (2) and the reactive tank being connected with micro- injector (2) by capillary (3), reactive tank is double cylinder, internal layer post
Space of the face (12) between outer layer cylinder is divided into three thermostatic chambers, respectively high-temperature denatured thermostatic chamber (9), process annealing thermostatic chamber
(10) and middle temperature extension thermostatic chamber (11), equably spiral wrap is on internal layer cylinder (12) for capillary (3), internal layer cylinder
Upper, middle and lower three pieces of micro- heating chips are set, what micro- heating chip be connected by pad (5) and with pad (5) be uniformly distributed three add
Thermode (6) is constituted, and heating electrode (6) is stretched out in internal layer cylinder (12) to three thermostatic chambers, and the centre of heating electrode (6) is left
Temperature sensor (4) is provided with passage, passage, microcontroller (7) is connected by circuit (8) with three pieces of micro- heating chips.
2. DNA cloning device according to claim 1, it is characterised in that the internal layer cylinder (12) and three thermostatic chambers
Material be heat-barrier material.
3. DNA cloning device according to claim 1, it is characterised in that described capillary (3) passes through different flat-temperature zones
Seal washer is used during domain.
4. DNA cloning device according to claim 1, it is characterised in that described capillary (3) is in internal layer cylinder (12)
Upper spiral winding 30~45 finally extends after enclosing at the top of reactive tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720063883.XU CN206385166U (en) | 2017-01-19 | 2017-01-19 | A kind of DNA cloning device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720063883.XU CN206385166U (en) | 2017-01-19 | 2017-01-19 | A kind of DNA cloning device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206385166U true CN206385166U (en) | 2017-08-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720063883.XU Active CN206385166U (en) | 2017-01-19 | 2017-01-19 | A kind of DNA cloning device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109234156A (en) * | 2018-09-28 | 2019-01-18 | 杨帆 | A kind of biology temperature control device |
| CN114134033A (en) * | 2021-12-06 | 2022-03-04 | 广东润鹏生物技术有限公司 | PCR thermal cycling device and control method |
-
2017
- 2017-01-19 CN CN201720063883.XU patent/CN206385166U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109234156A (en) * | 2018-09-28 | 2019-01-18 | 杨帆 | A kind of biology temperature control device |
| CN114134033A (en) * | 2021-12-06 | 2022-03-04 | 广东润鹏生物技术有限公司 | PCR thermal cycling device and control method |
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