It is a kind of new to take liquid culture bag
Technical field
The utility model is related to Bioexperiment instrument field, more particularly to a kind of new to take liquid culture bag.
Background technology
Culture bag be it is a kind of can support the cell culture container of high-density growth by what special gas permeable material made, because of it
Using transparent material, can direct observation of cell state under the microscope, and its gas exchange area is big, compared with blake bottle,
Can obtain greater number of cell culture bags be applied to culture suspension cell, so be now widely used in T cell, NK cells and
The culture of the human lymphocytes such as NKT cells.Current culture bag is essentially all an overall sack.Collected in cell
Cheng Zhong, some are not that disposable collecting is complete, or the certain cell of collection is detected can thus there be sample cross contamination
Probability.
Utility model content
In order to solve the above technical problems, the utility model provide it is a kind of it is new take liquid culture bag, using a main bag body
The structure connected with branched bag, solves conventional cell culture bag and takes the problem of liquid has cross pollution probability.
To reach above-mentioned purpose, the technical solution of the utility model is as follows:It is a kind of new to take liquid culture bag, including main bag
Body, feed tube, drain pipe and at least one branch bag;The feed tube and drain pipe are located at the two ends of the main bag body respectively;Institute
State and the brasnch conduct with convex port is connected with main bag body;The branch with female port is connected with the branch bag to lead
Pipe;The convex port is with the female port equipped with the nut for sealing;The main bag body by convex port with it is recessed
The mating connection of shape port connects the branch bag.
It is preferred that, the brasnch conduct is located at the middle part of the main bag body with the junction of the main bag body.
It is preferred that, the feed tube is 2, respectively positioned at the both sides of main bag body.
It is preferred that, the drain pipe is 3, the adjacent drain pipe spaced set.
It is preferred that, the branch bag is 1-5.
It is preferred that, also including two grades of branch bags;The brasnch conduct with convex port is connected with the branch bag;Described two grades
The brasnch conduct with female port is connected with branch bag;The branch bag is connected by the mating connection of convex port and female port
Logical two grades of branch bag.
It is preferred that, the drain pipe is connected with anti-pollution connector;The anti-pollution connector includes connecting conduit, male end
Mouth, compartment;The compartment is made up of PP films, is internally formed cylindrical cavity;One end of the cavity is provided with taper
Opening, and be connected by connecting conduit with drain pipe;The other end of the cavity is provided with the taper with the tapered opening
Plug;Convex port is connected by connecting conduit in the middle part of the cavity.
It is preferred that, the brasnch conduct being connected with the main bag body is provided with the skirt stretched out at the outer of convex port
Side;The width of the shirt rim is 1-2.5cm;The shirt rim is made up of EVA material.
It is preferred that, the nearly main bag body end of the brasnch conduct is provided with pipe clamp.
As further improvement of the utility model, also including three-level branch bag;It is connected with two grades of branch bag with convex
The brasnch conduct of shape port;The brasnch conduct with female port is connected with the three-level branch bag;Two grades of branch bag passes through
Convex port connects the three-level branch bag with the mating connection of female port.
By above-mentioned technical proposal, the beneficial effect of technical solutions of the utility model is:The utility model is rational in infrastructure, adopts
The mode being connected with a main bag body and branched bag, it is adaptable to a variety of experimental occasions, can be effective using more flexible
Avoid the pollution probability for frequently taking liquid to cause in experimentation.Feed tube and drain pipe are located to the two ends of main bag body respectively,
Feed liquor is carried out relatively independently and the process of liquid is taken, it is to avoid mutually pollution.And drain pipe connection anti-pollution connector, can effectively it keep away
Exempt from repeatedly to take liquid process to cause sample in main bag body to pollute main bag body;The setting of shirt rim, can prevent brasnch conduct
In use, port contact is to other pollution sources, and prevents from touching table top, it is ensured that the security of port.
Brief description of the drawings
, below will be to embodiment in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
Or the accompanying drawing to be used needed for description of the prior art is briefly described, it should be apparent that, drawings in the following description are only
It is some embodiments of the present utility model, for those of ordinary skill in the art, is not paying the premise of creative work
Under, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation disclosed in the utility model embodiment.
Fig. 2 is the structural representation of the utility model main bag body.
Fig. 3 is the structural representation of the utility model anti-pollution connector.
Fig. 4 is the structural representation of brasnch conduct of the utility model with shirt rim.
Fig. 5, Fig. 6 are the structural representations disclosed in the utility model embodiment.
Numeral and the corresponding component title represented by letter in figure:
1. the drain pipe of 2. feed tube of main bag body 3.
4. 5. anti-pollution connectors of bag 6. of brasnch conduct
7. the conical plug of 8. tapered opening of compartment 9.
10. the shirt rim of 11. nut of connecting conduit 12.
13. two grades of branch bags
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is carried out
Clearly and completely describe, it is clear that described embodiment is only a part of embodiment of the utility model, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belongs to the scope of the utility model protection.
Embodiment 1
With reference to Fig. 1 to Fig. 4, new liquid culture bag, including main bag body 1, feed tube are taken the utility model discloses a kind of
2nd, drain pipe 3 and a branch bag 5;Feed tube 2 and drain pipe 3 are located at the two ends of main bag body 1 respectively.Feed tube 2 is preferably 2,
It is located at the both sides of main bag body 1 respectively.Drain pipe 3 is preferably 3, the adjacent spaced set of drain pipe 3.It is connected with main bag body 1
Brasnch conduct 4 with convex port;The brasnch conduct 4 with female port is connected with branch bag 5;Convex port and female end
Mouth is equipped with the nut 11 for sealing.Brasnch conduct 4 is located at the middle part of main bag body 1 with the junction of main bag body 1.Main bag body 1
Pass through convex port and the mating connection connected component bag 5 of female port.Pipe can be provided with the nearly end of main bag body 1 of brasnch conduct 4
Folder, is dammed when being separated so as to branch bag 5 with main bag body 1.
In order to further reduce the possibility that sample pollutes, anti-pollution connector can be set in the exit of drain pipe 3
6;Specifically, anti-pollution connector 6 includes connecting conduit 10, convex port, compartment 7;Compartment 7 is made up of PP films, internal
Form cylindrical cavity;One end of cavity is provided with tapered opening 8, and is connected by connecting conduit 10 with drain pipe 3;In cavity
The other end be provided with conical plug 9 with tapered opening 8;Convex port is connected by connecting conduit 10 in the middle part of cavity.When taking liquid,
Keep conical plug 9 to be separated with tapered opening 8, open convex port, progress takes liquid;Take after liquid terminates, first close cone with conical plug 9
Shape opening 8, then closes convex port with pipe clamp or nut 11.In addition, the brasnch conduct 4 being connected with main bag body 1 can be
The shirt rim 12 stretched out is set at the outer of convex port;The width of shirt rim 12 is preferably 1-2.5cm;Shirt rim 12 is by EVA material
It is made, it is possibility to have PP goods PE materials are made.Port contact can so be reduced to the possibility of other pollution sources.
Embodiment 2
With reference to Fig. 5, main bag body 1 can use structure same as Example 1, mainly include main bag body 1, feed tube 2, go out
Liquid pipe 3 and multiple bags 5, preferably 2-5.It is by the way of two branch bags 5 such as Fig. 5.Two branch bags 5 are located at master respectively
2 sides of bag.Feed tube 2 and drain pipe 3 are located at the two ends of main bag body 1 respectively;Point with convex port is connected with main bag body 1
Branch conduit 4;The brasnch conduct 4 with female port is connected with branch bag 5;Convex port is with female port equipped with for sealing
Nut 11;Main bag body 1 passes through convex port and the mating connection connected component bag 5 of female port.
In use, being referred to following steps:
1. culture bag is put on operating desk, it is ensured that table top is neatly without sharppointed article, and feed tube 2 carries out feed liquor, Ran Houyong
Cap stopper or pipe clamp closing feed tube 2, carry out cell culture.
2. brasnch conduct 4 is clamped with pipe clamp.
3. the female port of brasnch conduct 4 and convex port are separated, screwed respectively with nut 11, branch bag 5 and main bag body 1
Separation.
4. main bag body 1 and another bag 5 proceed culture.
5. collecting the cell in branch bag 5, relevant subsequent experimental implementation is carried out.
6. female port and convex port on another brasnch conduct 4 are separated, screwed respectively with nut 11, obtain second
Branch bag 5.
Embodiment 3
With reference to Fig. 6, two grades of branch bags 13 are employed in the present embodiment, other structures are similar to Example 1.The present embodiment is in branch
The brasnch conduct 4 with convex port is connected with bag 5;The brasnch conduct 4 with female port is connected with two grades of branch bags 13;
Branch bag 5 connects two grades of branch bags 13 with the mating connection of female port by convex port.
Embodiment 4
Embodiment 4 sets up three-level branch bag 5 on the basis of embodiment 3;It is connected with two grades of branch bags 13 with convex port
Brasnch conduct 4;The brasnch conduct 4 with female port is connected with three-level branch bag 5;Two grades of branch bag 13 by convex port with
The mating connection connection three-level branch bag 5 of female port.
By above-mentioned specific embodiment, the beneficial effects of the utility model are:The utility model is rational in infrastructure, using a master
The mode that bag 1 and branched bag 5 are connected, it is adaptable to a variety of experimental occasions, using more flexible, it is possible to prevente effectively from
Frequently the pollution probability that liquid is caused is taken in experimentation.Feed tube 2 and drain pipe 3 are located to the two ends of main bag body 1, phase respectively
To independently carrying out feed liquor and taking the process of liquid, it is to avoid mutually pollution.And the connection anti-pollution connector 6 of drain pipe 3, can effectively it keep away
Exempt from repeatedly to take liquid process to cause sample in main bag body 1 to pollute main bag body 1;The setting of shirt rim 12, can prevent branch
Conduit 4 in use, port contact to other pollution sources.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or new using this practicality
Type.A variety of modifications to these embodiments will be apparent for those skilled in the art, determine herein
The General Principle of justice can in other embodiments be realized in the case where not departing from spirit or scope of the present utility model.Cause
This, the utility model is not intended to be limited to the embodiments shown herein, and is to fit to and principles disclosed herein
The most wide scope consistent with features of novelty.