CN206202966U - A kind of plating medium packaging bag - Google Patents
A kind of plating medium packaging bag Download PDFInfo
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- CN206202966U CN206202966U CN201621120727.4U CN201621120727U CN206202966U CN 206202966 U CN206202966 U CN 206202966U CN 201621120727 U CN201621120727 U CN 201621120727U CN 206202966 U CN206202966 U CN 206202966U
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- bag
- plating medium
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- middle level
- sack
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Abstract
The utility model discloses a kind of plating medium packaging bag, including outer bag, middle level bag and inner layer bag, the bag chamber for accommodating plating medium is formed in inner layer bag, outer bag, middle level bag and inner layer bag are compound membrane bag, the sack sealing of outer bag, middle level bag and inner layer bag, is provided with drier in inner layer bag.The utility model uses three layers of packaging bag, often one layer of external packing can be removed into a clean rank, avoid and cause potential pollution because culture medium external packing enters clean control area, drier is placed with inner layer bag, the steam of plating medium generation can be absorbed, reduce plating medium surface steam influence properties of product, drier is to adsorb vapor by Physical, infiltration rate relaxes will not drastically absorb water, avoid the quality for excessively absorbing water and influenceing plating medium, steam is absorbed by drier and causes that the moisture of plating medium reaches dynamic equilibrium, the quality that ensure that product and the shelf-life for extending product.
Description
Technical field
The utility model is used for medium package field, more particularly to a kind of plating medium packaging bag.
Background technology
Aseptic pre- filling agar medium flat board is used for the microorganism monitoring of clean environment, is produced using specific culture medium
Product, culture is monitored to the flcating germ in clean environment, settling bacteria, body surface bacterium etc., to determine the cleaning of clean environment
Whether grade meets the requirements.Most of composition of medium matrix is water, because plating medium is in storage and transportation
Can there is temperature fluctuation, culture medium produces aqueous vapor in packaging bag when temperature is higher, aqueous vapor condensation after temperature is reduced suddenly
It is attached in packaging bag and plating medium into water droplet, the miscellaneous bacteria outside plating medium is possible to flow into flat board with condensed water
Cause culture medium microbiological contamination in culture medium.And packaging material and packing method are weighed to the quality of above culture medium and aseptic playing to pass
The effect wanted.
Country's plating medium packing method mainly has vacuum bag packages, heat shrink films at present, although can prevent packaging
Extraneous bacterium enters in culture medium flat plate culture medium, but effectively steam unnecessary in packaging bag can not be excluded or absorbed, because
In this plating medium storage transportation, vapor cohesion can produce more aqueous water in plating medium, influence product
Can, simultaneously because containing residues such as culture mediums in the aqueous water of flat board generation, may be to the cleaning using plating medium
Zone pollutes；Also part producer is packed using ventilating packaging bag, although can prevent to pack extraneous bacterium and enter culture medium
In plating medium, but plating medium is constantly lost in storage transportation reclaimed water branch through venting bags, causes flat board
The serious dehydration influence product quality of culture medium, so as to substantially reduce the shelf-life of culture medium.
Existing culture medium flat plate culture medium internal layer packaging is paper plastic bag structure, bag face all opaque structure on one side it is saturating
Air film, another side for all-transparent structure PET/PE composite membranes, so as to cause cannot complete observation plating medium inside shape
, using dialysis paper membrane, its membrane material easily absorbs moisture for most of state, opaque section ventilated membrane, once culture medium steam exists
Occurs condensed water phenomenon during mercury dropped, then paper material moisture-sensitive of dialysing causes membrane aperture to become big or paper sheet break, causes dirt
Dye, influences the other membrane material low intensity of flat board quality, and service life is not long.
Breathable to reduce steam in order to pack, current most domestic producer uses paper plastic bag as internal layer packaging,
And paper plastic bag packaging there is another problem, the effect not intercepted to hydrogen peroxide steam exactly, part pharmaceutical factory can be
The flat board for there was only internal layer packaging is sterilized with hydrogen peroxide steam in isolator, if inner layer bag does not have to hydrogen peroxide
Impermeable ability, part hydrogen peroxide steam will be caused to penetrate into packaging bag so as to influence the Detection results of culture medium.
The user high for purification rank requirement, the packaging to culture medium proposes requirement higher, and certain customers make
Need to be operated in clean control area during with microorganism plating medium, so during clean area is entered,
Definitely avoid the regional pollution caused by culture medium and packaging carry disease germs into workspace.
Utility model content
To solve the above problems, the utility model provides a kind of plating medium packaging bag, and it can completely cut off external microbe
Pollute, prevent hydrogen peroxide from permeating and plating medium can be kept in the reclaimed water point dynamic equilibrium of storage transportation.
The utility model solves the technical scheme that its technical problem used：A kind of plating medium packaging bag, including
Outer bag, the middle level being sleeved in outer bag bag and the inner layer bag being sleeved in the bag of middle level, form receiving flat in the inner layer bag
The bag chamber of plate culture medium, the outer bag, middle level bag and inner layer bag be can the infiltration of anti-hydrogen peroxide compound membrane bag, it is described outer
The sack sealing of layer bag, middle level bag and inner layer bag, is provided with drier in inner layer bag.
The improvement of technical solutions of the utility model is further used as, the sack of the outer bag, middle level bag and inner layer bag is equal
With heat-sealable area, the sack of inner layer bag is provided with below heat-sealable area can close or open the slide fastener or clip chain of sack.
It is further used as the improvement of technical solutions of the utility model, the sack side of the outer bag, middle level bag and inner layer bag
Edge is equipped with tearable mouth.
The improvement of technical solutions of the utility model is further used as, the drier is silica-gel desiccant, the drier
It is placed in desiccant package bags, desiccant package bags are non-woven bag.
The improvement of technical solutions of the utility model is further used as, also including the paper plastic bag being placed in after folding in inner layer bag,
The sack of the paper plastic bag has viscose bar.
The improvement of technical solutions of the utility model is further used as, the middle level bag is provided with Product labelling below sack
Paste area.
The improvement of technical solutions of the utility model is further used as, the outer bag is provided with irradiation below sack and indicates
Area is pasted in agent.
The improvement of technical solutions of the utility model is further used as, the outer bag, middle level bag and inner layer bag are transparent
Nylon PE compound membrane bags.
The beneficial effects of the utility model：The utility model is packed using three layers of packaging bag to plating medium, bag
Inner bag, middle bag and outer bag are included, simple structure is easy to use, be particularly suitable for purifying and require that rank user high uses, can often entered
Enter a clean rank and remove one layer of external packing, it is to avoid because culture medium external packing enters clean control area cause potential dirt
Dye, is placed with drier in inner layer bag, the steam that plating medium is produced in storage and transportation can be absorbed, so as to subtract
Plating medium surface steam influence properties of product are lacked, drier is to adsorb vapor by Physical, and infiltration rate relaxes
Will not drastically absorb water, it is to avoid excessive water suction and influence the quality of plating medium, plating medium is absorbed by drier and is existed
The steam produced in storage transportation causes that the moisture of plating medium reaches dynamic equilibrium, it is ensured that the quality of product and prolong
The shelf-life of product is grown.
Brief description of the drawings
The utility model is described in further detail below in conjunction with the accompanying drawings：
Fig. 1 is the utility model inner layer bag structural representation；
Fig. 2 is the utility model middle level bag structural representation；
Fig. 3 is the utility model outer bag structural representation.
Specific embodiment
Referring to figs. 1 to Fig. 3, that show the concrete structure of the preferred embodiment of the utility model.Below will specifically
The design feature of bright each element of the utility model, and if being described to during direction (upper and lower, left and right, before and after), be with
Structure shown in Fig. 1 is that, with reference to description, but actually used direction of the present utility model is not limited thereto.
The utility model provides a kind of plating medium packaging bag, including outer bag 1, is sleeved in outer bag 1
Layer bag 2 and the inner layer bag 3 being sleeved in middle level bag 2, form the bag chamber for accommodating plating medium in the inner layer bag 3, described outer
Layer bag 1, middle level bag 2 and inner layer bag 3 be can the infiltration of anti-hydrogen peroxide compound membrane bag, the outer bag 1, middle level bag 2 and interior
The layer left side of bag 3, the right and base heat-sealing, sack is reserved in top margin, and the outer bag 1, middle level bag 2 and inner layer bag 3 are in packaging
After the completion of seal sack, is provided with drier in inner layer bag 3, the drier is preferably silica-gel desiccant, is placed with inner layer bag 3
Silica-gel desiccant, can absorb the steam that plating medium is produced in storage and transportation, so as to reduce flat board culture
Primary surface steam influences properties of product, and silica-gel desiccant is to adsorb vapor by Physical, and infiltration rate relaxes will not be drastically
Water suction, it is to avoid excessive water suction and influence the quality of plating medium, plating medium is absorbed by drier and is transported in storage
During the steam that produces cause that the moisture of plating medium reaches dynamic equilibrium, it is ensured that the quality of product and extend product
Shelf-life.The drier is placed in desiccant package bags, and desiccant package bags are non-woven bag, the anti-anti-avulsion of non-woven fabrics material
Junk pollutes culture medium.
Wherein, the outer bag 1, middle level bag 2 and inner layer bag 3 are transparent nylon PE compound membrane bags, and user is to built-in
Plating medium is very clear.Three layers of packaging bag use anti-hydrogen peroxide infiltration composite (main material be nylon with
PE), hydrogen peroxide steam can effectively be isolated, it is ensured that plating medium will not during with hydrogen peroxide vapor sterilization
Influence the quality of culture medium.
The sack of the outer bag 1, middle level bag 2 and inner layer bag 3 is respectively provided with heat-sealable area, and the sack of inner layer bag 3 is in heat-sealable area
Lower section and be provided with sack 3cm and can close or open the slide fastener or clip chain 4 of sack, can after slide fastener or clip chain 4 are sealed
Fixed with by whole bag plating medium, slided in bag so as to avoid plating medium, user is in this bag of plating medium
Slide fastener or clip chain closing seam can be preserved when not being finished, be easy to use next time.
The outer bag 1, middle level bag 2 and inner layer bag 3 are equipped with tearable mouth 5 below the sack at the left and right edges of 1.5cm,
It is easy to user to open bag to use.
Also include the paper plastic bag being placed in inner layer bag 3 after folding, the sack of the paper plastic bag has viscose bar.Paper plastic bag can
Transfer and culture after being sampled for plating medium, prevent the extraneous pollution for causing in transfer and incubation.
During encapsulation, 10 plating mediums are stacked cylindrical, be mounted in the bag chamber of inner layer bag 3, while in internal layer
Silica-gel desiccant one is put into bag 3 to wrap, fold paper plastic bag one, then close slide fastener or clip chain 4, due to slide fastener or clip chain 4
Height it is suitable with the height of 10 pieces of flat boards, therefore plating medium can be fixed after slide fastener or clip chain 4 are closed, prevent from putting down
Plate culture medium is slided in transportation is preserved, and seals sack after slide fastener or the good seal of clip chain 4.
Inner layer bag 3 is packed into middle level bag 2, Product labelling is affixed on the product mark of hair at the 8cm of sack lower section after sack heat-sealing
Sign and paste area 6.Middle level bag 2 is packed into outer bag 1, the irradiation that indicator is affixed at the 8cm of sack lower section will be irradiated after sack heat-sealing
Indicator pastes area 7.
Drier soaking effect is assessed
By taking soy peptone agar medium as an example, TSA 90mm plating mediums are randomly selected（Three layers of aseptic packaging）Respectively
Three batches, are weighed and are recorded to drier and the bag each plating medium respectively after unpacking, and perform mark, Ran Houchong
It is new with three layers of packaging bag good seal, it is irradiated after, sample is placed in 50 DEG C（The thermal extremes transport of simulation culture medium）4 in insulating box
After it（It was observed that the globule is no longer absorbed in bag）, the drier of each bag sample is taken out, weigh and record, by the training of each flat board
Foster base is uncapped and is placed in 10min in 50 DEG C of insulating boxs, is weighed and is recorded after drying surface moisture, compares plating medium experiment front and rear
Weight difference, calculates drier water suction and reaches saturation rear plate culture medium fluid loss.From Tables 1 and 2, transported through simulation
Afterwards, the actual water suction total amount of drier is 7.06g~7.93g（90mm plating mediums）, the dehydration in every bag of plating medium
14.10~15.30g of total amount, higher than the water absorption of drier, illustrates that drier absorbs water in packaging bag and has reached saturation state,
Therefore drier will not excessively absorb water.Testing result is shown in Tables 1 and 2.
The TSA 90mm plating medium drier water absorption of table 1 is assessed
| Sample | G/ bags of weight before simulation transport | G/ bags of weight after simulation transport | Increase g/ bags of weight |
| 20,140,410 1-C of TSA lot numbers | 31.40 | 38.79 | 7.39 |
| 20,140,414 1-C of TSA lot numbers | 31.40 | 38.46 | 7.06 |
| 20,140,423 1-C of TSA lot numbers | 32.00 | 39.93 | 7.93 |
| Average value | 31.6 | 39.06 | 7.46 |
The TSA 90mm plating medium culture mediums fluid loss of table 2 is assessed
| Sample | G/ bags of weight before simulation transport | G/ bags of weight after simulation transport | G/ bags of dehydration weight |
| 20,140,410 1-C of TSA lot numbers | 470.30 | 455.0 | 15.30 |
| 20,140,414 1-C of TSA lot numbers | 442.49 | 427.27 | 15.22 |
| 20,140,423 1-C of TSA lot numbers | 415.60 | 401.40 | 14.10 |
| Average value | 442.80 | 427.89 | 14.87 |
Biology effect assessment after addition drier
TSA 90mm plating mediums（Three layers of aseptic packaging）After irradiated sterilizing, sample is placed in 4 in 50 DEG C of insulating boxs
After it（It was observed that the globule is no longer absorbed in bag）, drier water absorption reach saturation assess plating medium quality, will
Object bacteria is made the bacteria suspension containing bacterium number about 300~1000cfu/mL, 100 μ L is taken respectively and is inoculated in tested flat board, is connect per bacterial strain
Two flat boards are planted, bacterium cultivates 18-24h in 30-35 DEG C, and fungi cultivates 48-72h in 20-25 DEG C；Each object bacteria is inoculated with two
Plating medium.Observation colony growth situation and counting, and record result.As shown in Table 3, addition silica-gel desiccant can effectively be inhaled
The steam that level board is produced, condensed water is significantly reduced compared with drier sample and paper plastic bag vacuum packaging is not added with.Can by table 4
Know, after plating medium is through simulation transport, drier absorbs water and reaches saturation, the physical and chemical index and biological indicator of culture medium
Meet standard requirement, do not interfere with the quality of product.TSA 90mm plating mediums（Three layers of aseptic packaging）Three batch additions
The packing method of drier is significantly less than the packing method for being not added with drier through the content of condensed water in simulation transport back pkt..It is comprehensive
Upper described, the packing method for adding silica-gel desiccant is effectively reduced the condensation that plating medium is produced in transportation is preserved
Water, while will not also be adversely affected to the quality of culture medium.Testing result is shown in Table 3 tables 4.
Drier soaking effect compares after the simulation transport of table 3
| Sample | Plus desiccant package | It is not added with desiccant package | Paper plastic bag is vacuum-packed |
| 20,140,410 1-C of TSA lot numbers | Less solidifying water | More condensed water | More condensed water |
| 20,140,414 1-C of TSA lot numbers | Less solidifying water | More condensed water | More condensed water |
| 20,140,423 1-C of TSA lot numbers | Less solidifying water | More condensed water | More condensed water |
The TSA 90mm plating mediums of table 4（Three layers of aseptic packaging）Through simulation transport rear plate performance evaluation result
The anti-hydrogen peroxide infiltration assessment of inner layer bag
300ml deionized waters, heat-sealing will be loaded in inner layer bag；The sack of good seal is put into the peroxidating that concentration is 35%
1.5 hours in hydrogen solution, and make blank；Sack is taken out into wash clean, is dried, detected with hydrogen peroxide detector bar（Merck
0.5-25mg/l）The concentration of hydrogen peroxide of sack reclaimed water；Simultaneously with sack and paper without anti-hydrogen peroxide infiltration function
Bag as control, every group of sample do three it is parallel.As shown in Table 5, without the sack and paper of anti-hydrogen peroxide infiltration function
Bag hydrogen peroxide osmotic concentration be respectively 10-15mg/L and> 25mg/L；And this inner layer bag permeates material using anti-hydrogen peroxide
Material, the osmotic concentration of hydrogen peroxide is 0.5-2 mg/L, illustrates that the infiltration of hydrogen peroxide can be effective against.Testing result is shown in Table
5。
The anti-hydrogen peroxide permeability assessment result of the packaging bag of table 5
| Detection sample | Three average results of sample bag |
| Blank | 0 |
| Anti- hydrogen peroxide permeates packaging bag | 0.5-2 mg/L |
| Normal packet is packed | 10-15 mg/L |
| Paper plastic bag | > 25mg/L |
| 35% hydrogenperoxide steam generator | > 25mg/L |
Inner layer bag physics and chemistry and biological evaluation after hydrogen peroxide infiltration
By TSA 90mm plating mediums（Three layers of aseptic packaging）Three batches remove outer two-layer packaging bag, in only
It is put into 1.5 hours in the hydrogenperoxide steam generator that concentration is 35% in the case of layer bag；Sack is taken out into wash clean, is dried, by flat board
After culture medium takes out, the performance of culture medium is assessed.Sense organ Physico-chemical tests, observe culture medium outward appearance, concern culture medium color and
Clarity, the pH value determination at 25 DEG C ± 1 DEG C surveys two plating mediums, averages.Biological Detection, by object bacteria system
Into the bacteria suspension containing bacterium number about 300~1000cfu/mL, 100 μ L are taken respectively and is inoculated in tested flat board, put down for two per inoculation
Plate, bacterium cultivates 18-24h in 30-35 DEG C, and fungi cultivates 48-72h in 20-25 DEG C；Each object bacteria is inoculated with two flat boards.See
Colony growth situation and counting are examined, and records result.As shown in Table 6, be through concentration 35% hydrogenperoxide steam generator in soak 1.5
The physical and chemical index and biological indicator of hour TSA 90mm three batches of plating medium meet standard requirement, do not interfere with product
The quality of product.
Testing result is shown in Table 6.
The impact evaluation result of the TSA 90mm plating medium resistive to hydrogen peroxide effects of table 6
In sum, the utility model provides a kind of packing method of plating medium, can completely cut off external microbe dirty
Contaminate, prevent hydrogen peroxide from permeating and plating medium can be kept in the reclaimed water point dynamic equilibrium of storage transportation, there is very extensive
Application prospect.
Certainly, the invention is not limited to above-mentioned implementation method, and those of ordinary skill in the art are without prejudice to originally
Equivalent variations or replacement can be also made on the premise of utility model spirit, these equivalent modifications or replacement are all contained in the application
In claim limited range.
Claims (8)
1. a kind of plating medium packaging bag, it is characterised in that：Including outer bag, the middle level being sleeved in outer bag bag and suit
Inner layer bag in the bag of middle level, forms the bag chamber for accommodating plating medium in the inner layer bag, the outer bag, middle level bag and interior
Layer bag be can the infiltration of anti-hydrogen peroxide compound membrane bag, the sack sealing of the outer bag, middle level bag and inner layer bag, inner layer bag
Inside it is provided with drier.
2. plating medium packaging bag according to claim 1, it is characterised in that：The outer bag, middle level bag and internal layer
The sack of bag is respectively provided with heat-sealable area, and the sack of inner layer bag is provided with below heat-sealable area can close or open the slide fastener or folder of sack
Chain.
3. plating medium packaging bag according to claim 1, it is characterised in that：The outer bag, middle level bag and internal layer
The sack edge of bag is equipped with tearable mouth.
4. plating medium packaging bag according to claim 1, it is characterised in that：The drier is silica-gel desiccant,
The drier is placed in desiccant package bags, and desiccant package bags are non-woven bag.
5. plating medium packaging bag according to claim 1, it is characterised in that：Also include being placed in inner layer bag after folding
Paper plastic bag, the sack of the paper plastic bag has viscose bar.
6. plating medium packaging bag according to claim 1, it is characterised in that：The middle level bag sets below sack
There is Product labelling to paste area.
7. plating medium packaging bag according to claim 1, it is characterised in that：The outer bag sets below sack
There is irradiation indicator to paste area.
8. plating medium packaging bag according to claim 1, it is characterised in that：The outer bag, middle level bag and internal layer
Bag is transparent nylon PE compound membrane bags.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2015210551528 | 2015-12-16 | ||
| CN201521055152 | 2015-12-16 |
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| Publication Number | Publication Date |
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| CN206202966U true CN206202966U (en) | 2017-05-31 |
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ID=58762326
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| CN201621120727.4U Active CN206202966U (en) | 2015-12-16 | 2016-10-13 | A kind of plating medium packaging bag |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110920970A (en) * | 2019-11-28 | 2020-03-27 | 上海韵飞生物科技有限公司 | Packaging method of cell culture consumable meeting GMP (good manufacturing practice) requirements |
| CN113928724A (en) * | 2021-07-28 | 2022-01-14 | 上海韵飞生物科技有限公司 | Cell culture consumable and packaging method thereof |
| CN114084525A (en) * | 2021-12-14 | 2022-02-25 | 浙江华康药业股份有限公司 | Method for preventing agglomeration of sugar alcohol powder during storage |
| CN114132561A (en) * | 2021-12-02 | 2022-03-04 | 上海诺狄生物科技有限公司 | Full-automatic three-layer negative pressure packaging system and method for prefabricated culture medium |
| CN114132562A (en) * | 2021-12-02 | 2022-03-04 | 上海诺狄生物科技有限公司 | Dull and stereotyped full automatic production line of prefabricated culture medium |
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2016
- 2016-10-13 CN CN201621120727.4U patent/CN206202966U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110920970A (en) * | 2019-11-28 | 2020-03-27 | 上海韵飞生物科技有限公司 | Packaging method of cell culture consumable meeting GMP (good manufacturing practice) requirements |
| CN113928724A (en) * | 2021-07-28 | 2022-01-14 | 上海韵飞生物科技有限公司 | Cell culture consumable and packaging method thereof |
| CN114132561A (en) * | 2021-12-02 | 2022-03-04 | 上海诺狄生物科技有限公司 | Full-automatic three-layer negative pressure packaging system and method for prefabricated culture medium |
| CN114132562A (en) * | 2021-12-02 | 2022-03-04 | 上海诺狄生物科技有限公司 | Dull and stereotyped full automatic production line of prefabricated culture medium |
| CN114084525A (en) * | 2021-12-14 | 2022-02-25 | 浙江华康药业股份有限公司 | Method for preventing agglomeration of sugar alcohol powder during storage |
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