CN206033772U - Catch device of magnetic particle mark cell - Google Patents
Catch device of magnetic particle mark cell Download PDFInfo
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- CN206033772U CN206033772U CN201620986252.0U CN201620986252U CN206033772U CN 206033772 U CN206033772 U CN 206033772U CN 201620986252 U CN201620986252 U CN 201620986252U CN 206033772 U CN206033772 U CN 206033772U
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- capture
- sample
- magnetic particle
- container
- particle labels
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Abstract
The utility model provides a catch device of magnetic particle mark cell, includes that first container, sample collecting pit, sample catch pond and magnetic field generator. First container includes first appearance chamber to hold sample and magnetic particle, the sample collecting pit is used for collecting the sample after catching, the sample is caught the pond and is included the catcher way, and the catcher is said including entry and export, entry and first appearance chamber intercommunication, export and sample collecting pit intercommunication, magnetic field generator sends magnetic field in catcher way. The device provided by the utility model, be applicable to the cell of catching the mark magnetic particle, adopt the common utensil in laboratory can realize equipment by oneself, low cost, the acquisition speed is fast, and convenient to use is along with using along with the dress.
Description
Technical field
The utility model is related to a kind of experimental provision for implementing life science, more particularly to a kind of capture magnetic particle labels
The device of cell.
Background technology
Circulating cancer cells (Circulating Tumor Cells, CTC) are present in each quasi-cancer cell in the circulatory system
It is referred to as.CTC once enters suitable histoorgan, you can bud into new metastatic cancer focus.Therefore the inspection to CTC
Survey, the assessment of the early stage etiological diagnosis for cancer patient, therapeutic strategy formulation, curative effect and prognosis all has very important meaning
Justice.But the content of CTC is very low in human peripheral blood, add in blood containing the red blood cell than much higher times of CTC, leucocyte, blood
How platelet etc., be effectively enriched with or capture these CTC in substantial amounts of " background " cell, becomes the key technology of CTC detections.
As numbers of the CTC in peripheral blood is many less more than normal haemocyte, many laboratories are all being ground in the world
Study carefully its method for separating, capturing and be enriched with.Such as：Separated according to the physical size of CTC.Due to CTC cells mean size about
L7 ± 1.5 μm, will be big than normal red blood cell, granulocyte, lymphocyte and monocyte, therefore, respective aperture can be used
Millipore filter or microwell chips carry out physical separation, then CTC is purified (such as by the follow-up wash-outs of Jing or the mode such as backflow：J
Chromatogr A.2007,1162 (2), 154～16l, Nat Protoc.2014,9 (3), 694～710,
200910198495.2 with 201210181873.0 etc.).Although the purity of such method purifies and separates CTC is higher, due to which
Processing technology is complicated, and flow rate of liquid is slower, and separating a sample needs larger amount of sample (more than 7.5ml), average time-consuming up to 4
More than hour, it is difficult to meet the clinical demand of quick detection.
Second method is adsorbed using surface-functionalized film or pipe-line system, so as to carry out CTC separation.The method is
Tumour antigen is all expressed based on most of CTC cells, antibody functionalized, sample flow after dilution is used in film/pipe surface
When Jing films or pipe surface, the surface antigen of CTC can be combined with corresponding antibody, so as to realize CTC capture (such as：
201310139673.0,201410153597.3 and 201210193145.9 etc.).Such method is needed first by film or pipe surface
Carry out it is antibody functionalized, due to different films or pipe surface property it is different, the combination effect of antibody it is difficult to ensure that, therefore cause
The acquisition performance deviation of CTC is very big.Additionally, there are the antibody in film/pipe surface to react with the antigen on CTC surfaces, need
Certain reaction temperature and time, such device are just effective only when flow rate of liquid is extremely slow, and need thermostat.Therefore,
Such technology application prospect is equally weaker.
The third method is to adopt digitlization capture systems (such as：201410818225.8) and based on the micro- of graphene oxide
Fluidic chip capture systems are (such as：Nat Nano.2013,8 (10), 735～741), and it is based on chip culture integral system
(Oncotarget.2014,75 (15), supp., 1～15) etc., all because complex process, flow process are loaded down with trivial details and using reasons such as inconvenience,
It is difficult to promote the use of in reality.
4th kind of method be based on immune magnetic separation technique, such separation method it is many with bulky grain magnetic bead (micron order or with
On) based on, cancer cell capture is carried out after magnetic bead surfaces carry out corresponding antibodies coupling, then CTC is purified into by Jing elution processes
Come (such as：J Transl Med.2011,9 (1)：1～8, Lab on a Chip.2014,14 (1)：57～62).Such technology
Advantage is the relatively high magnetism of magnetic bead, and separating power is higher, but carries out corresponding biology inspection (such as：Culture, nucleic acid extraction and exempt from
Epidemic disease histochemical staining etc.) operation before magnetic bead must be eluted using chemical method, the effect of the chemical substance of this process is not only right
CTC produces serious chemically or physically damage, it is also possible to disturb follow-up Biological Detection process.Also detection receives with magnetic in addition
Based on rice corpuscles (such as：201310614556.5) and subsequent detection during be not required to through elution process, but it is such detection make
Magnetic nanometer is γ-Fe2O3。
Said method provides beneficial reference for the separation of CTC, but remain a need for these methods convenient to carry out one kind or
Several devices.
Utility model content
The purpose of this utility model is to provide a kind of device of capture magnetic particle labels cell, is easy to catch by magnetic field
Obtain the cell of magnetic particle labels.
A kind of device of capture magnetic particle labels cell that the utility model is provided, including：
First container, which includes the first cavity volume, to accommodate sample and magnetic-particle；
Sample collection pond, its sample being used for after collecting capture；
Analyte capture pond, which includes capturing cavity, and capture cavity includes an inlet and an outlet, and entrance is connected with the first cavity volume,
Outlet is connected with sample collection pond；
Magnetic field generator, which sends magnetic field and in capture cavity.
Higher for sample concentration or viscosity, even if applying external force, the still relatively low sample of flow velocity can be using entering to sample
The mode of row dilution increases flow velocity, such as：After by sample and magnetic-particle mixing, then dilute.In order to improve the Automated water of device
It is flat so as to enter back into analyte capture pond Jing after the sample of magnetic particle labels is blended with dilution.
The device of another kind of capture magnetic particle labels cell that the utility model is provided, including：
First container, which includes the first cavity volume, to accommodate sample and magnetic-particle；
Sample collection pond, its sample being used for after collecting capture；
Second container, which includes the second cavity volume, to accommodate dilution；
Analyte capture pond, which includes capturing cavity, and capture cavity includes an inlet and an outlet, and the outlet of catcher road is received with sample
Ji Chi is connected；
Blender, which is connected with the first container and entrance respectively；
Magnetic field generator, which sends magnetic field and in capture cavity.
The sample not high for capture rate, can still enter sample collection pond, and need sample in the capture at first initial stage
Collection liquid in collecting pit, is captured again.In order to obtain capture after cell, also allow for control change analyte capture pond and
The sample contamination for causing, arranges current divider on the stream between analyte capture pond and sample collection pond.
The device of another kind of capture magnetic particle labels cell that the utility model is provided, including：
First container, which includes the first cavity volume, to accommodate sample and magnetic-particle；
Sample collection pond, its sample being used for after collecting capture；
Second container, which includes the second cavity volume, to accommodate dilution；
Analyte capture pond, which includes capturing cavity, and capture cavity includes an inlet and an outlet；
Current divider, its respectively with sample collection pond and outlet；
Blender, which is connected with the first container and entrance respectively；
Magnetic field generator, which sends magnetic field and in capture cavity.
Blender of the present utility model, is also connected with second container.
Current divider of the present utility model, also including the second shunting outlet, for collecting captured Jing magnetic particle labels
Sample (such as：The cell of Jing magnetic particle labels, bacterium, or other pathogen).
For ease of the connection of all parts, device of the present utility model, also including Rule pipe, respectively with the first container and mixed
Clutch connects, and second container and blender connection, or sample collection pond is connected with current divider.
The beneficial effect that technical solutions of the utility model are realized：
The device of the capture magnetic particle labels cell that the utility model is provided, it is adaptable to which capture marks the thin of magnetic-particle
Born of the same parents, are capable of achieving voluntarily to assemble using the common utensil in laboratory, and with low cost, acquisition speed is fast, easy to use, with
Dress.
The device of the capture magnetic particle labels cell that the utility model is provided, except the capture that can be used for magnetic cycle cancer cell
Outward, it may also be used for the capture of the related substances such as pathogenic bacteria, fungi, virion, bacteriophage and excretion body.Except can be used for patient
The capture of related substances in the blood and body fluid in source, it may also be used for the efficient capture of related substances in vitro culture thing.
Description of the drawings
Fig. 1 is an example structure schematic diagram of the device that the utility model captures magnetic particle labels cell；
Fig. 2 is another example structure schematic diagram of the device that the utility model captures magnetic particle labels cell.
Specific embodiment
The technical solution of the utility model is described in detail below in conjunction with accompanying drawing.The utility model embodiment is only to illustrate this
The technical scheme of utility model and it is unrestricted, although being described in detail to the utility model with reference to preferred embodiment, ability
Domain it is to be appreciated by one skilled in the art that can modify to the technical scheme of utility model or equivalent, and do not take off
From the spirit and scope of technical solutions of the utility model, which all should be covered in right of the present utility model.
Fig. 1 is an example structure schematic diagram of the device that the utility model captures magnetic particle labels cell.Such as Fig. 1 institutes
Show, the present embodiment device includes the first container 100 and sample collection pond 200.First container 100 includes that the first cavity volume (does not show
Go out), to accommodate sample and magnetic-particle.The sample that sample collection pond 200 is used for after collecting capture.Its bag of analyte capture pond 300
Capture cavity 310 is included, capture cavity includes entrance 311 and outlet 312, and entrance 311 is connected with the first cavity volume, exports 312 and sample
Product collecting pit is connected.Magnetic field generator 400 is (such as：The ferromagnetic rod of rubidium) be arranged at capture cavity 310 periphery, its send magnetic field and in
Capture cavity 310 (capture cavity is located in magnetic field), makes the sample of Jing magnetic particle labels deflect and be adsorbed by magnetic field,
And do not deflect without the sample of magnetic-particle and enter sample collection pond 200.
The device provided for ease of the present embodiment is also applied for the relatively low sample of flow velocity, and provides automatization level, this reality
Apply example and another kind of device is also provided.Fig. 2 is an example structure of the device that the utility model captures magnetic particle labels cell
Schematic diagram.As shown in Fig. 2 the present embodiment device includes the first container 100, second container 500 and sample collection pond 200.First
Container 100 includes the first cavity volume, to accommodate sample and magnetic-particle.Second container 500 includes the second cavity volume (not shown), to hold
Receive dilution.
The sample that sample collection pond 200 is used for after collecting capture.Analyte capture pond 300 it include capture cavity 310, capture
Cavity includes entrance 311 and outlet 312, and outlet 312 is connected with sample collection pond 200.Blender 700 respectively with the first container
100th, second container 500 is connected with entrance 311.Magnetic field generator 400 is (such as：The ferromagnetic rod of rubidium) it is arranged at the outer of capture cavity 310
In week, which sends magnetic field and in capture cavity 310, the sample of Jing magnetic particle labels is deflected and is adsorbed by magnetic field, and
Do not deflect without the sample of magnetic-particle and enter sample collection pond 200.
The sample not high for capture rate, can still enter analyte capture pond 200 in the capture at first initial stage, can be by sample
Sample liquid in capture pond 200 carries out second capture again by entrance.Cause in order to avoid being conveniently replaceable analyte capture pond
Sample contamination, on the stream between analyte capture pond and sample collection pond arrange current divider 800, its respectively with sample collection
200 connect with outlet 312, also including the second shunting outlet 830, for collecting the cell of Jing magnetic particle labels.
The device that the present embodiment is provided is easy to capture the cell of Jing magnetic particle labels and be enriched with, and can adopt
The common utensil in laboratory is realized voluntarily assembling, with low cost, is suitable for disposable application, and easy to use, acquisition speed is fast,
It is easy to carry out follow-up biological experiment operation to capturing cell in time.
Claims (8)
1. it is a kind of capture magnetic particle labels cell device, it is characterised in that include
First container, which includes the first cavity volume, to accommodate sample and magnetic-particle；
Sample collection pond, its sample being used for after collecting capture；
Analyte capture pond, which includes capturing cavity, and described capture cavity includes an inlet and an outlet, described entrance with it is described
First cavity volume is connected, and described outlet is connected with described sample collection pond；
Magnetic field generator, which sends magnetic field and in described capture cavity.
2. it is according to claim 1 capture magnetic particle labels cell device, it is characterised in that also including second container,
Which includes the second cavity volume, to accommodate dilution.
3. it is according to claim 1 capture magnetic particle labels cell device, it is characterised in that also including blender, its
Connect with the first described container and the entrance respectively.
4. it is according to claim 3 capture magnetic particle labels cell device, it is characterised in that also including second container,
Which includes the second cavity volume, and to accommodate dilution, described blender is also connected with described second container.
5. it is according to claim 4 capture magnetic particle labels cell device, it is characterised in that also including Rule pipe, point
It is not connected with the first described container and described blender, and described second container and described blender connection.
6. it is according to claim 1 capture magnetic particle labels cell device, it is characterised in that also including current divider, its
Respectively with described sample collection pond and described outlet.
7. it is according to claim 6 capture magnetic particle labels cell device, it is characterised in that described current divider is also
Including the second shunting outlet, for collecting the sample of captured Jing magnetic particle labels.
8. it is according to claim 6 capture magnetic particle labels cell device, it is characterised in that also including Rule pipe, point
It is not connected with described current divider with described sample collection pond.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620986252.0U CN206033772U (en) | 2016-08-29 | 2016-08-29 | Catch device of magnetic particle mark cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620986252.0U CN206033772U (en) | 2016-08-29 | 2016-08-29 | Catch device of magnetic particle mark cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206033772U true CN206033772U (en) | 2017-03-22 |
Family
ID=58302424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620986252.0U Expired - Fee Related CN206033772U (en) | 2016-08-29 | 2016-08-29 | Catch device of magnetic particle mark cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206033772U (en) |
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2016
- 2016-08-29 CN CN201620986252.0U patent/CN206033772U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170322 Termination date: 20170829 |