CN205893283U - Still oxygen suppliment cell culture jar of fixed bed - Google Patents
Still oxygen suppliment cell culture jar of fixed bed Download PDFInfo
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- CN205893283U CN205893283U CN201620864849.8U CN201620864849U CN205893283U CN 205893283 U CN205893283 U CN 205893283U CN 201620864849 U CN201620864849 U CN 201620864849U CN 205893283 U CN205893283 U CN 205893283U
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- oxygen
- fixed bed
- foamless oxygen
- gas
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Abstract
The utility model discloses a still oxygen suppliment cell culture jar of fixed bed utilizes the effect of concentration difference through still apparatus of oxygen supply, permeates the oxygen suppliment in to the culture solution by the micropore of hydrophobic permeability cell, can not produce unnecessary bubble, also not excessively oxygen suppliment, the production of stopping the bubble, if because in the hydrophobic permeability cell of the vapor that high temperature produced infiltration in the culture solution for when atmospheric pressure in the still apparatus of oxygen supply risees, then the accessible open exhaust pipe the valve with unnecessary gas outgoing. The utility model discloses simple structure is novel in design, the problem of the easy gassing of directly ventilating in having solved the cell culture jar from the source, very big improvement biological cell culture technical merit.
Description
Technical field
The utility model is related to biological cell culture technique, more particularly, to a kind of fixed bed foamless oxygen-supplying bioreactor.
Background technology
Cell culture technology is also cell clone technology, and the regular noun in biology is cell culture technology.No matter
For whole biotechnology, or for the biological cloning technology of one of them, cell culture be all one essential
Process, the extensive clone of cell culture inherently cell.Cell culture technology can be trained through a large amount of by a cell
Form the many cells for simply unicellular or few differentiation, substantial amounts of cell or its metabolite are obtained by cell culture.
Because biological product be all from cell get it is possible to say cell culture technology be biotechnology most crucial, most basic
Technology.Cell culture work has been widely used in the every field such as biology, medical science, new drug development, becomes most important base
One of plinth science.
The growth needs of cell are carried out in the culture tank of sealing, it is to avoid extraneous bacterium dirt infection；In order to provide cell growth
Necessary oxygen, needs to provide oxygen into the nutrient solution in culture tank, by pipeline directly to tank body more than existing technology
Interior nutrient solution supply, is so easy for producing substantial amounts of bubble in nutrient solution, thus affecting the growth of cell, because cell
Cultivation cycle is typically longer, for preventing culture tank inner product from pressing through many bubbles, needs constantly to exclude bubble to outside tank, not only increases
The risk of the extraneous bacterium dirt of infection, can cause the waste of mass propgation liquid simultaneously.
Utility model content
The purpose of this utility model is to provide a kind of bioreactor of novel structural principle, solves prior art and exists
Shortcoming, during cell culture, it is to avoid in nutrient solution produce bubble.
The utility model adopts the following technical scheme that realization:
A kind of fixed bed foamless oxygen-supplying bioreactor, including tank body, basket packed bed, rabbling mechanism and foamless oxygen-supplying dress
Put.
Described basket packed bed is placed in tank body, and for annular barrel structure, top cover and bottom are perforated plate construction, and center sets
Some gas-liquid inverting chambers, described gas-liquid inverting chamber top is provided with sliding bearing fixed seat, and described basket packed bed bottom is provided with props up
Frame.
Described rabbling mechanism includes floating centrifugal lifting stirring-head and driving means, described floating centrifugal lifting stirring
It is provided with the cavity that bottom is opened, described floating centrifugal lifting stirring-head is provided with some centrifugation pipelines in head.Described float from
Core type lifting stirring-head lower end is contained in sliding bearing fixed seat.
Described foamless oxygen-supplying device is arranged in gas-liquid inverting chamber, is provided with the air inlet pipe connecting with external air source and substantial amounts of
Hydrophobic, air-permeability pipe；Described hydrophobic, air-permeability pipe stretches in gas-liquid inverting chamber, upper end closed, and lower end is connected with foamless oxygen-supplying pipe.
Preferably, it is provided with universal joint between described stirring-head and the drive shaft of driving means.
Preferably, described foamless oxygen-supplying device is additionally provided with by the gas exhaust piping of Valve controlling.
Preferably, described hydrophobic, air-permeability pipe is fire resistant polymer hydrophobic permeable membrane.
Occupation mode of the present utility model is as follows:
Carrier is placed in basket packed bed, adds nutrient solution into tank body, nutrient solution liquid level is higher than the height of centrifugation pipeline
Degree, when sterilizing enters cell culture state after terminating, adds cell, starts driving means, drives the rotation of float lifting formula stirring-head
Turn, with the rotation of float lifting formula stirring-head, the nutrient solution in gas-liquid inverting chamber can elevated and centrifugation along stirring-head
Pipeline flows out, and forms negative pressure in gas-liquid inverting chamber, forces the nutrient solution in tank body to enter gas-liquid from gas-liquid inverting chamber bottom and hands over
Change chamber so that the nutrient solution in tank body circulates along gas-liquid inverting chamber.When nutrient solution flows through gas-liquid inverting chamber, still confession
Oxygen in oxygen device can be entered in nutrient solution by the micropore permeation on hydrophobic, air-permeability pipe in the presence of concentration difference, completes to training
The oxygen supply of nutrient solution, nutrient solution, when flowing through basket packed bed, oxygen molecule is supplied cell growth.
Advantageous Effects of the present utility model are: the utility model utilizes the work of concentration difference by foamless oxygen-supplying device
With, from the micropore of hydrophobic, air-permeability pipe to nutrient solution in permeate oxygen supply, the dissolved oxygen in culture medium controls still confession by dissolved oxygen electrode
The gas inlet valve of oxygen device, is accurately controlled do, ph, and prevents the generation of bubble；If because high temperature produces in nutrient solution
Raw vapor penetrates in hydrophobic, air-permeability pipe so that when the water yield in hydrophobic, air-permeability pipe raises, then can pass through to open gas exhaust piping
Valve unnecessary water is discharged.The utility model structure simple designs are novel, solve bioreactor direct from source
Ventilation is also easy to produce the problem of a large amount of bubbles, greatly improves biological cell culture technique level.
Brief description
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the structural representation of basket packed bed；
Fig. 3 is the top view of basket packed bed.
Specific embodiment
By the following description to embodiment, will more contribute to public understanding the utility model, but can't
Specific embodiment given by applicant is considered as the restriction to technical solutions of the utility model, any special to part or technology
The definition levied be changed and/or overall structure is made with form and immaterial conversion is regarded as technology of the present utility model
The protection domain that scheme is limited.
The present embodiment provides a kind of fixed bed foamless oxygen-supplying bioreactor, including tank body 1, basket packed bed 2, mixer
Structure 3 and foamless oxygen-supplying device 4.
Described basket packed bed 2 is placed in tank body 1, and for annular barrel structure, top cover and bottom are perforated plate construction, center
The gas-liquid inverting chamber 2.1 being provided with, described gas-liquid inverting chamber 2.1 top is provided with sliding bearing fixed seat 2.2, described basket packed bed
2 bottoms are provided with support 2.3.
Described rabbling mechanism 3 includes float lifting formula stirring-head 3.1 and driving means 5, described float lifting formula stirring-head
It is provided with the cavity 3.3 opened bottom, outside is provided with three centrifugation pipelines 3.2 in 3.1；Under described float lifting formula stirring-head 3.1
End is contained in sliding bearing fixed seat 2.2.
Described foamless oxygen-supplying device 4 is arranged in gas-liquid inverting chamber, is provided with by the gas exhaust piping 4.1 of Valve controlling and outer
The air inlet pipeline 4.2 of portion's source of the gas connection and substantial amounts of hydrophobic, air-permeability pipe 4.3；Described hydrophobic, air-permeability pipe 4.3 stretches into gas-liquid inverting chamber
In 2.1, upper end closed, lower end is connected with foamless oxygen-supplying pipe.
It is provided with universal joint 6 between described float lifting formula stirring-head 3.1 and the drive shaft of driving means 5.
The occupation mode of the present embodiment is as follows:
Carrier is placed in basket packed bed 2, adds nutrient solution into tank body 1, nutrient solution liquid level is higher than centrifugation pipeline 3.2
Height, after end subject to sterilization enter cell culture state when, add cell, start driving means 5, drive float lifting formula stir
Mix 3.1 rotation, with the rotation of float lifting formula stirring-head 3.1, the nutrient solution in gas-liquid inverting chamber 2.1 can elevated and edge
The outflow of centrifugation pipeline 3.2, form negative pressure in gas-liquid inverting chamber 2.1, force the nutrient solution in tank body 1 to enter gas from bottom
Liquid inverting chamber 2.1 is so that the nutrient solution in tank body 1 circulates along gas-liquid inverting chamber 2.1.Exchange when nutrient solution flows through gas-liquid
During chamber 2.1, the oxygen in foamless oxygen-supplying device 4 can pass through the micropore permeation on hydrophobic, air-permeability pipe 4.3 in the presence of concentration difference
Enter in nutrient solution, complete the oxygen supply to nutrient solution, nutrient solution, when flowing through basket packed bed 2, oxygen molecule is supplied cell growth.
The present embodiment by foamless oxygen-supplying device 4 utilize concentration difference effect, from hydrophobic, air-permeability pipe 4.3 micropore to culture
Infiltration oxygen supply in liquid, the dissolved oxygen in culture medium controls the gas inlet valve of foamless oxygen-supplying device by dissolved oxygen electrode, can be accurate
Control do, ph, and prevent the generation of bubble；If because the vapor that high temperature produces penetrates into hydrophobic, air-permeability pipe 4.3 in nutrient solution
Interior so that when the water yield in foamless oxygen-supplying device 4.3 raises, then can be by opening the valve of gas exhaust piping 4.1 by unnecessary water
Discharge.The present embodiment structure simple designs are novel, solve the problems, such as that bioreactor ventilation is also easy to produce bubble, pole from source
Big improves biological cell culture technique level.
Certainly, the utility model can also have other various embodiments, without departing substantially from the utility model spirit and its essence
In the case of, those of ordinary skill in the art can make various corresponding changes according to the utility model and deform, but this
Corresponding change and deformation all should belong to the scope of the claims appended by the utility model a bit.
Claims (8)
1. a kind of fixed bed foamless oxygen-supplying bioreactor, including tank body, basket packed bed, rabbling mechanism and foamless oxygen-supplying dress
Put, it is characterized by: described basket packed bed is placed in tank body, and center is provided with gas-liquid inverting chamber, described gas-liquid inverting chamber top
It is provided with sliding bearing fixed seat；Described rabbling mechanism includes floating centrifugal lifting stirring-head and driving means, described floating from
Core type lifting stirring-head lower end is contained in sliding bearing fixed seat；Described foamless oxygen-supplying device is arranged in gas-liquid inverting chamber.
2. a kind of fixed bed foamless oxygen-supplying bioreactor according to claim 1, it is characterized by: described basket packed bed
For annular barrel structure, top cover and bottom are perforated plate construction.
3. a kind of fixed bed foamless oxygen-supplying bioreactor according to claim 1, it is characterized by: described basket packed bed
Bottom is provided with support.
4. a kind of fixed bed foamless oxygen-supplying bioreactor according to claim 1, it is characterized by: described floating centrifugal
It is provided with, in lifting stirring-head, the cavity that bottom is opened, outside is provided with some centrifugation pipelines.
5. a kind of fixed bed foamless oxygen-supplying bioreactor according to claim 1, it is characterized by: described foamless oxygen-supplying dress
Put and be provided with the air inlet pipe connecting with external air source and substantial amounts of hydrophobic, air-permeability pipe；Described hydrophobic, air-permeability pipe stretches into gas-liquid inverting chamber
Interior, upper end closed, lower end is connected with foamless oxygen-supplying pipe.
6. a kind of fixed bed foamless oxygen-supplying bioreactor according to claim 1, it is characterized by: described float lifting formula
It is provided with universal joint between stirring-head and the drive shaft of driving means.
7. a kind of fixed bed foamless oxygen-supplying bioreactor according to claim 5, it is characterized by: described foamless oxygen-supplying dress
Put and be additionally provided with by the gas exhaust piping of Valve controlling.
8. a kind of fixed bed foamless oxygen-supplying bioreactor according to claim 5, it is characterized by: described hydrophobic, air-permeability pipe
For fire resistant polymer hydrophobic hollow filter membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620864849.8U CN205893283U (en) | 2016-08-11 | 2016-08-11 | Still oxygen suppliment cell culture jar of fixed bed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620864849.8U CN205893283U (en) | 2016-08-11 | 2016-08-11 | Still oxygen suppliment cell culture jar of fixed bed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205893283U true CN205893283U (en) | 2017-01-18 |
Family
ID=57781225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620864849.8U Active CN205893283U (en) | 2016-08-11 | 2016-08-11 | Still oxygen suppliment cell culture jar of fixed bed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205893283U (en) |
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2016
- 2016-08-11 CN CN201620864849.8U patent/CN205893283U/en active Active
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |