CN205821356U - A kind of agitator for culture plant cell device - Google Patents
A kind of agitator for culture plant cell device Download PDFInfo
- Publication number
- CN205821356U CN205821356U CN201521079920.3U CN201521079920U CN205821356U CN 205821356 U CN205821356 U CN 205821356U CN 201521079920 U CN201521079920 U CN 201521079920U CN 205821356 U CN205821356 U CN 205821356U
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- CN
- China
- Prior art keywords
- rotary shaft
- cross tube
- fixedly connected
- utility
- agitator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
This utility model provides a kind of agitator for culture plant cell device, the single rotary unit of stirring structure in prior art is extended by this technical scheme, define upper and lower two-layer laterally to stir and longitudinally stirring, thus be obviously improved adequacy and the uniformity of stirring.In order to alleviate the produced fierce splashing in whipping process of this utility model agitator, this utility model is provided above disc baffles at cross tube, thus effectively prevent culture fluid and splash with produced by stirring and air-flow, on this basis, in order to eliminate a large amount of bubble, this utility model is provided with sheet glass below disc baffles, culture fluid bubble can produce when sheet glass rotates with rotating shaft certain shear action, thus contribute to eliminating bubble.This utility model achieves prominent technique effect with relatively simple technological improvement, and cost is relatively low simultaneously, be easily achieved, and therefore has prominent promotion prospect.
Description
Technical field
This utility model relates to culture plant cell equipment technical field, is specifically related to a kind of for plant cell training
Support the agitator of device.
Background technology
Culture plant cell is in vitro, and callus or other easy scattered tissues are placed in liquid training
Support and base carry out concussion cultivation, obtain being dispersed into free suspension cell, make cell proliferation by successive transfer culture,
Thus obtain a kind of technology of a large amount of cell colony.According to Object of Development, culture plant cell mainly has unicellular
Cultivating, monoploid is cultivated, Protoplast cuhnre etc.；According to culture systems can be divided into suspension culture, liquid culture,
Solid culture, Immobilized culture etc..
Plant cell batch production process in, the culture tank of scale is one of indispensable device,
The culture plant cell tank of prior art is broadly divided into gas-lifting type and the big class of stirring-type two, wherein airlift reactor
Dissolved oxygen is more abundant, but mass-transfer efficiency is relatively low and needs more complicated gas recirculation assembly and filtration sterilization ring
Joint；The application of stirring type bioreactor is the most extensive comparatively speaking, it is possible to fully ensures that the suspension of cell and has
Help promote culture fluid dissolved oxygen.But plant cell is different from the microorganism of free living existence, it is to mechanical shear stress
Toleration poor, if stirring paddle material hardness is relatively big, easily cause cell rupture dead, and if using simply
Softer stirring paddle is then difficult to ensure that the adequacy of stirring.If additionally, prior art meeting stirring adequacy
Require to typically require fiercer stirring or ventilation, perform to often lead to culture fluid during this generic operation in culture tank
Splash in tank, and other assemblies at culture tank top may be infected with, on the one hand have impact on culture efficiency the most also
The component operation such as the pH probe that is easily caused in tank body, temperature probe are abnormal.
Summary of the invention
This utility model aims to the technological deficiency of prior art, it is provided that a kind of for culture plant cell device
Agitator, to solve prior art causes culture fluid to splash for culture plant cell tank when high degree of agitation
Technical problem.
For realizing above technical purpose, this utility model by the following technical solutions:
A kind of agitator for culture plant cell device, including vertical rotary shaft, sheer pole, cross tube, turns
Dish, sponge sheet, through hole, coir rope, motor, horizontal rotary shaft, fan, disc baffles, vertical bar, glass
Sheet, wherein indulges rotary shaft, sheer pole, cross tube three all in hollow tubular, and sheer pole and cross tube hang down respectively
Direct-connected be connected in vertical rotary shaft, be interconnected inside vertical rotary shaft, sheer pole, cross tube three, sheer pole and
Being distributed with some through holes on cross tube, the bondage respectively of the two ends of sheer pole has coir rope, and the two ends of cross tube are respectively
Bondage has coir rope, motor to be fixedly connected on vertical interior of rotating shaft, horizontal rotary shaft one end is connected with motor reel,
The other end extend out to outside vertical rotary shaft, and rotating disk is fixedly connected on the other end of described horizontal rotary shaft, sponge sheet
Being fixedly connected on the edge of rotating disk, fan is fixedly connected on vertical interior of rotating shaft, and disc baffles is fixedly connected on
Vertical rotary shaft, the card of disc baffles is perpendicular with vertical rotary shaft, and disc baffles position is higher than cross tube, vertically
Bar is fixedly connected on below disc baffles, and sheet glass is fixedly connected on vertical bar lower end.
In above technical scheme, the single rotary unit that prior art stirs module is extended, and is formed
Upper and lower two-layer laterally stirs and longitudinally stirring, thus has been obviously improved adequacy and the uniformity of stirring.?
On the basis of this, this utility model is coir rope in horizontal blade two ends bondage, thus expands when blade rotates training
The sphere of action of nutrient solution, owing to the shape of coir rope is the most irregular, therefore, it is possible to promote the turbulent extent of culture fluid.
In order to alleviate the blade mechanical shear stress to plant cell, this utility model rotates sponge sheet and coir rope as motion
Unit, the two quality is the most soft, and the integrity of cell can be effectively ensured；What is more important, this practicality
Novel on the cross bar performing stirring, it is provided with some through holes, utilizes the fan being built in vertical rotary shaft to carry out simultaneously
Air-flow exports, thus avoids the direct collision of cell and cross bar, reduces mechanical shear stress.
In order to alleviate the produced fierce splashing in whipping process of this utility model agitator, this utility model exists
Cross tube be provided above disc baffles, thus effectively prevent culture fluid and fly produced by stirring and air-flow
Spattering, on this basis, in order to eliminate a large amount of bubble, this utility model is provided with sheet glass below disc baffles,
Culture fluid bubble can be produced certain shear action when sheet glass rotates with rotating shaft, thus contribute to eliminating
Bubble.This utility model achieves prominent technique effect with relatively simple technological improvement, simultaneously cost relatively low,
It is easily achieved, therefore there is prominent promotion prospect.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of this utility model embodiment 1 agitator；
Fig. 2 is the vertical section structure schematic diagram that rotary shaft indulged by this utility model embodiment 1 agitator；
In figure
1, vertical rotary shaft 2, sheer pole 3, cross tube 4, rotating disk
5, sponge sheet 6, through hole 7, coir rope 8, motor
9, horizontal rotary shaft 10, fan 11, disc baffles 12, vertical bar
13, sheet glass
Detailed description of the invention
Hereinafter detailed description of the invention of the present utility model will be described in detail.In order to avoid the most unnecessary
Details, in the examples below to belonging to known structure or function will not be described in detail.Following example
Used in approximating language can be used for quantitative expression, show can allow in the case of not changing basic function
Quantity has certain variation.In addition to being defined, technology used in following example and scientific terminology have and this
The identical meanings that utility model one of ordinary skill in the art are commonly understood by.
Embodiment 1
A kind of agitator for culture plant cell device, including vertical rotary shaft 1, sheer pole 2, cross tube
3, rotating disk 4, sponge sheet 5, through hole 6, coir rope 7, motor 8, horizontal rotary shaft 9, fan 10, plate-like
Baffle plate 11, vertical bar 12, sheet glass 13, wherein indulge rotary shaft 1, sheer pole 2, cross tube 3 three equal
In hollow tubular, sheer pole 2 and cross tube 3 are respectively perpendicular and are connected in vertical rotary shaft 1, vertical rotary shaft 1,
It is interconnected inside sheer pole 2, cross tube 3 three, sheer pole 2 and cross tube 3 is distributed with some logical
Hole 6, the bondage respectively of the two ends of sheer pole 2 has coir rope 7, the bondage respectively of the two ends of cross tube 3 to have coir rope 7,
It is internal that motor 8 is fixedly connected on vertical rotary shaft 1, and horizontal rotary shaft 9 one end is connected with motor 8 axle, another
It is outside that end extend out to vertical rotary shaft 1, and rotating disk 4 is fixedly connected on the other end of described horizontal rotary shaft 9, sponge
Sheet 5 is fixedly connected on the edge of rotating disk 4, and it is internal that fan 10 is fixedly connected on vertical rotary shaft 1, and plate-like is kept off
Plate 11 is fixedly connected on vertical rotary shaft 1, and the card of disc baffles 11 is perpendicular with vertical rotary shaft 1, and plate-like is kept off
Plate 11 position is higher than cross tube 3, and vertical bar 12 is fixedly connected on below disc baffles 11, sheet glass 13
It is fixedly connected on vertical bar 12 lower end.
On the basis of above technical scheme, meet following condition:
The blowing direction of described fan is downward.
The aperture of described through hole 6 is 1mm.
Described rotating disk 4 is between sheer pole 2 and cross tube 3.
Embodiment 2
A kind of agitator for culture plant cell device, including vertical rotary shaft 1, sheer pole 2, cross tube
3, rotating disk 4, sponge sheet 5, through hole 6, coir rope 7, motor 8, horizontal rotary shaft 9, fan 10, plate-like
Baffle plate 11, vertical bar 12, sheet glass 13, wherein indulge rotary shaft 1, sheer pole 2, cross tube 3 three equal
In hollow tubular, sheer pole 2 and cross tube 3 are respectively perpendicular and are connected in vertical rotary shaft 1, vertical rotary shaft 1,
It is interconnected inside sheer pole 2, cross tube 3 three, sheer pole 2 and cross tube 3 is distributed with some logical
Hole 6, the bondage respectively of the two ends of sheer pole 2 has coir rope 7, the bondage respectively of the two ends of cross tube 3 to have coir rope 7,
It is internal that motor 8 is fixedly connected on vertical rotary shaft 1, and horizontal rotary shaft 9 one end is connected with motor 8 axle, another
It is outside that end extend out to vertical rotary shaft 1, and rotating disk 4 is fixedly connected on the other end of described horizontal rotary shaft 9, sponge
Sheet 5 is fixedly connected on the edge of rotating disk 4, and it is internal that fan 10 is fixedly connected on vertical rotary shaft 1, and plate-like is kept off
Plate 11 is fixedly connected on vertical rotary shaft 1, and the card of disc baffles 11 is perpendicular with vertical rotary shaft 1, and plate-like is kept off
Plate 11 position is higher than cross tube 3, and vertical bar 12 is fixedly connected on below disc baffles 11, sheet glass 13
It is fixedly connected on vertical bar 12 lower end.
Above embodiment of the present utility model is described in detail, but described content is the most of the present utility model
Preferred embodiment, not in order to limit this utility model.All done in application range of the present utility model appoint
What amendment, equivalent and improvement etc., within should be included in protection domain of the present utility model.
Claims (4)
1. the agitator for culture plant cell device, it is characterised in that include vertical rotary shaft (1),
Sheer pole (2), cross tube (3), rotating disk (4), sponge sheet (5), through hole (6), coir rope (7),
Motor (8), horizontal rotary shaft (9), fan (10), disc baffles (11), vertical bar (12),
Sheet glass (13), wherein indulges rotary shaft (1), sheer pole (2), cross tube (3) three all in hollow
Tubulose, sheer pole (2) and cross tube (3) are respectively perpendicular and are connected in vertical rotary shaft (1), vertical rotary shaft
(1), sheer pole (2), it is interconnected inside cross tube (3) three, sheer pole (2) and cross tube (3)
On be distributed with some through holes (6), the two ends bondage respectively of sheer pole (2) has coir rope (7), cross tube
(3) it is internal that the bondage respectively of two ends has coir rope (7), motor (8) to be fixedly connected on vertical rotary shaft (1),
It is outside that horizontal rotary shaft (9) one end is connected with motor (8) axle, the other end extend out to vertical rotary shaft (1),
Rotating disk (4) is fixedly connected on the other end of described horizontal rotary shaft (9), and sponge sheet (5) is fixedly connected on
On the edge of rotating disk (4), it is internal that fan (10) is fixedly connected on vertical rotary shaft (1), disc baffles (11)
Being fixedly connected on vertical rotary shaft (1), the card of disc baffles (11) is perpendicular with vertical rotary shaft (1), dish
Shape baffle plate (11) position is higher than cross tube (3), and vertical bar (12) is fixedly connected on disc baffles (11)
Going up below, sheet glass (13) is fixedly connected on vertical bar (12) lower end.
Agitator for culture plant cell device the most according to claim 1, it is characterised in that institute
The blowing direction stating fan is downward.
Agitator for culture plant cell device the most according to claim 1, it is characterised in that institute
The aperture stating through hole (6) is 1mm.
Agitator for culture plant cell device the most according to claim 1, it is characterised in that institute
State rotating disk (4) to be positioned between sheer pole (2) and cross tube (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521079920.3U CN205821356U (en) | 2015-12-18 | 2015-12-18 | A kind of agitator for culture plant cell device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521079920.3U CN205821356U (en) | 2015-12-18 | 2015-12-18 | A kind of agitator for culture plant cell device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205821356U true CN205821356U (en) | 2016-12-21 |
Family
ID=58147630
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521079920.3U Expired - Fee Related CN205821356U (en) | 2015-12-18 | 2015-12-18 | A kind of agitator for culture plant cell device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205821356U (en) |
-
2015
- 2015-12-18 CN CN201521079920.3U patent/CN205821356U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161221 Termination date: 20171218 |