CN205774568U - A kind of micro flow control chip device - Google Patents
A kind of micro flow control chip device Download PDFInfo
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- CN205774568U CN205774568U CN201620714716.2U CN201620714716U CN205774568U CN 205774568 U CN205774568 U CN 205774568U CN 201620714716 U CN201620714716 U CN 201620714716U CN 205774568 U CN205774568 U CN 205774568U
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- substrate
- magnet coil
- collecting tank
- flow control
- control chip
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Abstract
The utility model discloses a kind of micro flow control chip device, including: the first substrate and the second substrate, described first substrate upper surface is provided with plane magnet coil, and described first substrate lower surface is provided with multiple downward opening collecting tank, and described collecting tank is positioned at immediately below described plane magnet coil；Described second substrate upper surface is provided with fluid intake, access road, collecting region passage, exit passageway and the fluid issuing being sequentially communicated；And described collecting region passage is positioned at the underface of multiple described collecting tank.Therefore, a kind of micro flow control chip device that this utility model provides, by arranging collecting tank and microchannel on two substrates, and plane magnet coil is set on collecting tank top, such that it is able to utilize the magnetic field that plane magnet coil produces, and control size of current, it is possible to realize cells various in flowing through microchannel are separated and collected.
Description
Technical field
This utility model relates to micro fluidic chip technical field, particularly relates to a kind of micro flow control chip device.
Background technology
Microfluidic chip technology, also known as chip lab, is a kind of profit proposed by Sweden's research worker the nineties in 20th century
With micro-processing technology, by separation involved in biochemical reaction, sample, react and the basic step such as monitoring is integrated into area
For on the chip of squares several centimetres and realize whole-process control.In the separation process of biological cell, use at present based on immunity magnetic
The micro-fluidic chip of property isolation technics.The principle of this technology utilizes surface coupling specificities Anti-tumor labelling molecular antibody
Immunomagnetic beads captures the cell expressing corresponding antigens, adds stagnant for magnetic bead-cell conjugate the most outside under the effect of electric magnet
Stay in micro-fluidic chip passage, reach the purpose of target cell and unlabeled cells sorting.
Of the prior art Magnet is utilized to divide cellifugal micro-fluidic chip, it is common that permanent magnet to be set on chip and carries
The magnetic field of confession.But, the magnetic field provided is provided, its magnetic field intensity is relatively strong, and magnetic field immobilizes, it is impossible to regulation,
May be only available for single cell separation.
Utility model content
This utility model embodiment provides a kind of micro flow control chip device, solves micro-fluidic chip of the prior art
In the problem that cannot regulate of magnetic field.
A kind of micro flow control chip device that this utility model provides, including: the first substrate and the second substrate, described first base
Sheet upper surface is provided with plane magnet coil, and described first substrate lower surface is provided with multiple downward opening collecting tank, and described receipts
Collection groove is positioned at immediately below described plane magnet coil；Described second substrate upper surface is provided with fluid intake, the entrance being sequentially communicated
Passage, collecting region passage, exit passageway and fluid issuing；And described collecting region passage be positioned at multiple described collecting tank just under
Side.
It is also preferred that the left the described first substrate upper surface deposition of micro flow control chip device of the present utility model has insulating barrier, institute
Stating insulating barrier and be provided with through hole, described through hole is positioned at the center of described plane magnet coil, and described insulating barrier upper surface deposition has
Magnetic yoke structure, described magnetic yoke structure passes through described through hole and described plane magnet coil in the center of described plane magnet coil
Electrical contact.
It is also preferred that the left described first substrate of micro flow control chip device of the present utility model and described second substrate are glass,
Described insulating barrier is silicon dioxide, and described magnetic yoke structure is nickel dam.
To sum up, a kind of micro flow control chip device that this utility model embodiment provides, by arranging receipts on two substrates
Collection groove and microchannel, and plane magnet coil is set on collecting tank top, such that it is able to utilize the magnetic that plane magnet coil produces
, and control size of current, it is achieved that cells various in flowing through microchannel are separated and collected.
Accompanying drawing explanation
The structural representation of a kind of micro flow control chip device that Fig. 1 provides for this utility model embodiment；
First substrate lower surface structural representation of the micro-fluidic chip that Fig. 2 provides for this utility model embodiment；
First substrate upper surface structural representation of the micro-fluidic chip that Fig. 3 provides for this utility model embodiment.
Description of reference numerals: 100-the first substrate, 200-the second substrate, 101-plane magnet coil, 102-collecting tank,
103-through hole, 104-magnetic yoke structure, 201-fluid intake, 202-access road, 203-collecting region passage, 204-exit passageway,
205-fluid issuing.
Detailed description of the invention
Below in conjunction with the accompanying drawing in this utility model, the technical scheme of this utility model embodiment is carried out clear, complete
Description, it is clear that described embodiment is a part of embodiment of the present utility model rather than whole embodiments.Based on this
Embodiment in utility model, it is all that those of ordinary skill in the art are obtained on the premise of not making creative work
Other embodiments, all should belong to the scope of this utility model protection.
For the ease of understanding and explanation, describe, below by Fig. 1 to Fig. 3, the micro-fluidic chip that this utility model provides in detail
Device.Fig. 1 show a kind of micro flow control chip device structural representation that this utility model embodiment provides.As it is shown in figure 1, should
Micro flow control chip device may include that
First substrate 100 and the second substrate 200, the first substrate 100 upper surface is provided with plane magnet coil 101, the first base
Sheet 100 lower surface is provided with multiple downward opening collecting tank 102, and collecting tank 102 is positioned at immediately below plane magnet coil 101；
Second substrate 200 upper surface be provided be sequentially communicated fluid intake 201, access road 202, collecting region passage 203, exit passageway
204 and fluid issuing 205；And collecting region passage 203 is positioned at the underface of multiple collecting tank 102.
Concrete, the micro flow control chip device of this utility model embodiment offer, first can be in the first substrate 100 following table
The centre position in face arranges multiple downward opening collecting tank 102.Should be understood that collecting tank 102 can be evenly distributed in first
In the square of substrate 100 centre or rectangular area, the cross section of collecting tank 102 can be circular or oval.Then first
The upper surface of substrate 100 carries out the making of plane magnet coil 101, i.e. by micro-processing technology at the first substrate 100 upper surface
Aluminize or the metal level of other conductions, be then passed through etching etc., eventually form plane magnet coil 101 as depicted.Enter one
Step, deposit a layer insulating, such as silicon dioxide layer at the first substrate 100 upper surface so that each of plane magnet coil 101
All it is filled with an insulating layer between coil, and, leave through hole 103 in the center of plane magnet coil 101.Then can be
Deposition layer of metal nickel is continued so that metal nickel dam covers whole plane magnet coil 101, and is filled in through hole above insulating barrier
In 103, thereby may be ensured that sedimentary nickel makes electrical contact with in center with plane magnet coil 101, i.e. form magnetic yoke structure 104.
It addition, the upper surface at the second substrate 200 arranges microchannel, fluid intake 201 and fluid issuing 205 are i.e. set at two ends,
Access road 202, collecting region passage 203, exit passageway 204 is set gradually between fluid intake 201 and fluid issuing 205, and
And access road 202 is gradually broadened on the direction moving towards collecting region passage 203, exit passageway 204 goes out moving towards fluid
Mouth 205 directions become narrow gradually, and collecting region passage 203 arranges rectangular or square, and ensure and be positioned on the first substrate 100
Collecting tank 102 place rectangular or square when region coincide, i.e. when two substrate overlaps, collecting tank 102 region is just
It is positioned at well the surface of collection district passage 203.
In actual applications, first the immunomagnetic beads of antibody can be had to mix the fluid containing cell to be separated and coupling
Close, thus form the complex fluid containing cell-magnetic microsphere.Then this complex fluid is passed through under the effect of Micropump
Fluid intake 201, and enter microchannel.I.e. through access road 202 to collecting region passage 203.At this point it is possible to according to be separated
The parameters such as the weight of cell, regulation is passed through the size of current of plane magnet coil 101.After determining size of current, i.e. to plane electricity
Magnetic coil 101 is energized, thus produces magnetic field in plane magnet coil 101.Should be understood that under the action of a magnetic field, can will pass through
Cell-magnetic microsphere complex be collected in the collecting tank 102 of the first substrate 100.Finally, separated fluid is by outlet
Passage 203 flows out microchannel and is collected.Further, after all compound fluids flow through microchannel, it is also possible to close electric current, and
The cell collected is carried out the operations such as eluting, thus obtains pure cell to be separated.
Optionally, the first substrate 100 and the second substrate 100 can be glass, and insulating barrier can be silicon dioxide.
In sum, a kind of micro flow control chip device that this utility model embodiment provides, by setting on two substrates
Put collecting tank and microchannel, and plane magnet coil is set on collecting tank top, such that it is able to utilize plane magnet coil to produce
Magnetic field, and by controlled loading size of current on solenoid, it is possible to realize cells various in flowing through microchannel
Separate and collect.
Only several specific embodiments of the present utility model disclosed above, but, this utility model embodiment not office
Being limited to this, the changes that any person skilled in the art can think of all should fall into protection domain of the present utility model.
Claims (3)
1. a micro flow control chip device, it is characterised in that including: the first substrate (100) and the second substrate (200), described
One substrate (100) upper surface is provided with plane magnet coil (101), and described first substrate (100) lower surface is provided with multiple opening court
Under collecting tank (102), and described collecting tank (102) is positioned at immediately below described plane magnet coil (101)；Described second substrate
(200) upper surface be provided be sequentially communicated fluid intake (201), access road (202), collecting region passage (203), exit passageway
And fluid issuing (205) (204)；And described collecting region passage (203) is positioned at the underface of multiple described collecting tank (102).
A kind of micro flow control chip device the most according to claim 1, it is characterised in that the upper table of described first substrate (100)
Face deposition has insulating barrier, described insulating barrier to be provided with through hole (103), and described through hole (103) is positioned at described plane magnet coil
(101) center, described insulating barrier upper surface deposition has magnetic yoke structure (104), and described magnetic yoke structure (104) is at described plane electricity
The center of magnetic coil (101) is made electrical contact with described plane magnet coil (101) by described through hole (103).
A kind of micro flow control chip device the most according to claim 2, it is characterised in that described first substrate (100) and institute
Stating the second substrate (200) is glass, and described insulating barrier is silicon dioxide, and described magnetic yoke structure (104) is nickel dam.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620714716.2U CN205774568U (en) | 2016-07-07 | 2016-07-07 | A kind of micro flow control chip device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620714716.2U CN205774568U (en) | 2016-07-07 | 2016-07-07 | A kind of micro flow control chip device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205774568U true CN205774568U (en) | 2016-12-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620714716.2U Expired - Fee Related CN205774568U (en) | 2016-07-07 | 2016-07-07 | A kind of micro flow control chip device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205774568U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110653012A (en) * | 2019-10-18 | 2020-01-07 | 遵义市第一人民医院 | Multistage microfluidic chip device for sorting, enriching and detecting circulating tumor cells |
| CN116121063A (en) * | 2022-12-30 | 2023-05-16 | 山东大学 | Biochip for realizing magnetic field regulation and temperature monitoring and preparation method thereof |
-
2016
- 2016-07-07 CN CN201620714716.2U patent/CN205774568U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110653012A (en) * | 2019-10-18 | 2020-01-07 | 遵义市第一人民医院 | Multistage microfluidic chip device for sorting, enriching and detecting circulating tumor cells |
| CN116121063A (en) * | 2022-12-30 | 2023-05-16 | 山东大学 | Biochip for realizing magnetic field regulation and temperature monitoring and preparation method thereof |
| CN116121063B (en) * | 2022-12-30 | 2023-08-04 | 山东大学 | Biochip for realizing magnetic field regulation and temperature monitoring and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161207 Termination date: 20170707 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |