CN205774521U - A kind of late blight of potato sampling apparatus - Google Patents

Abstract

The open a kind of late blight of potato sampling apparatus of this utility model, including open-topped box body, it is covered on the lid of described opening, in box body, is placed with the fresh potato chips for covering disease sample, the interior top of described lid is provided with multiple depression bar, contacts with described fresh potato chips bottom described depression bar.In one embodiment of this utility model late blight of potato sampling apparatus, have employed openable seal box, in seal box, be placed with fresh potato chips.After field uses, immediately the late blight of potato disease sample sheared can be placed under fresh potato chips, make phytophthora invade potato chips and breed, until producing mycelia.Decrease the pollution of disease sample, be conducive to quickly obtaining substantial amounts of bacterial strain.

Description

A kind of late blight of potato sampling apparatus

Technical field

This utility model belongs to late blight of potato prevention and control field, relates to the sampling apparatus of phytopathy sample, is specifically related to horse The sampler of bell potato late blight.

Background technology

The late blight of potato PotatoLateBlightCaused by phytophthora infestans, cause potato haulm dead and tuber A kind of crushing oomycetes disease rotted.Pathogenic bacteria mainly survives the winter in potato block with mycelium, sows the potato block that carries disease germs, causes not germinateing Or be unearthed after germinateing and i.e. die, have be unearthed after become primary infection focus, disease portion produces Sporangium and borrows air-flow propagation to infect again, Form infection center, cause this disease and spread over a whole area from one point, spread rapidly expansion；Sporangium on sick leaf also can penetrate into rainwater or irrigation water Soil infects potato block, i.e. forms sick potato, become main source of infection in next year.Cold at night super-humid conditions, relative humidity are warmed up in pathogenic bacteria happiness day Under the conditions of more than 95%, 18-22 DEG C, favourable sporangial formation, cool (10-13 DEG C, keep 1-2 hour) has again water droplet to deposit Sprout at, favourable Sporangium and produce zoospore, warm (24-25 DEG C, continue 5-8 hour) with the presence of water droplet, favourable Sporangium Directly output germ tube, in the most rainy time, there was dampness in the air, or warm foggy under the conditions of morbidity weight.

A lot of currently for phytophthora infestans research, but monangial bacterial strain in the research of existing phytophthora infestans Separation method operation easier big, time-consuming for a long time, success rate is low.From fresh potato chips, directly separate phytophthora infestans, potato chips On miscellaneous bacteria be difficult to remove, separation process can cause a large amount of pollution, it is difficult to obtain pure phytophthora infestans for reality Test.

In order to solve above technical problem, prior art proposes a kind of phytophthora infestans monangial separation side Method, this separation method Inoculating needle is a little mycelia of picking from the potato leaf of morbidity, at the 2ml containing 200 μ l sterilized water Centrifuge tube stirs for several times, after mycelia on pin to be seeded and Sporangium are mixed into sterilized water, be the whirlpool of 1500rpm/min with rotating speed Rotation machine vortex 30s, is repeated twice；Sporangia suspension concentration is adjusted, by sporangia suspension concentration subsequently with blood counting chamber It is adjusted to 1/2 μ l；On a piece of microscope slide, placement three is with the coverslip of 75% ethanol sterilizing, respectively at three coverslipes Above dripping sporangia suspension, then microscopy under 10 × 10 power microscopes, if observing single Sporangium, then on drop Add a cover the rye (Secale cereale L.) culture medium of 0.8cm × 0.8cm, be then transferred in the empty culture dish of sterilizing, sealing；Culture dish is put K cryogenic treatment 2-3h to 4 DEG C of refrigerators, stimulates Sporangium release zoospore, and the environment being then transferred into 11 DEG C deposits 16- 18h, promotes that zoospore is sprouted, and finally places in the constant incubator of 18 DEG C and cultivates 4-10 days；Sporangium to be sprouted is black Produce mycelia in wheat culture block, transfer them to cultivate, in 18 DEG C of constant temperature on rye (Secale cereale L.) culture medium flat plate in superclean bench Incubator is cultivated, i.e. obtains the bacterial strain grown by single Sporangium.

During realizing this utility model, inventor finds that in aforementioned prior art, at least there are the following problems:

1, in traditional method, disease belt transect is returned laboratory, then disease sample is placed under fresh potato chips, after cultivating directly Separating phytophthora infestans from fresh potato chips, the miscellaneous bacteria on potato chips is difficult to remove, and can cause a large amount of dirt in separation process Dye, it is difficult to obtain pure phytophthora infestans strain for testing.

2, direct picking mycelia from blade, although decrease the pollution of miscellaneous bacteria, but mycelia is very little, when needing to spend more Between find Sporangium with microscope, separation efficiency is low.

Summary of the invention

In consideration of it, this utility model purpose is to provide one to be favorably improved the late blight of potato single Sporangium efficiency Late blight of potato sampling apparatus.

Inventor is by long-term exploration and trial, and experiment repeatedly and effort, constantly reforms and innovates, for solving Above technical problem, the technical scheme that this utility model provides is to provide a kind of late blight of potato sampling apparatus, including top The box body of opening, is covered on the lid of described opening, is placed with the fresh potato chips for covering disease sample in box body, described lid Interior top is provided with multiple depression bar, contacts with described fresh potato chips bottom described depression bar.

A detailed description of the invention according to this utility model late blight of potato sampling apparatus, the top of described depression bar is Cambered surface.

A detailed description of the invention according to this utility model late blight of potato sampling apparatus, described depression bar is big angle pressure Bar.

A detailed description of the invention according to this utility model late blight of potato sampling apparatus, the material of described depression bar is One in silica gel, glass, plastics.

A detailed description of the invention according to this utility model late blight of potato sampling apparatus, described box body and described lid Body is circle, and described box body is threadeded with described lid.

A detailed description of the invention according to this utility model late blight of potato sampling apparatus, the internal diameter of described box body is 5-10cm。

A detailed description of the invention according to this utility model late blight of potato sampling apparatus, described fresh potato chips are circle Sheet.

A detailed description of the invention according to this utility model late blight of potato sampling apparatus, the thickness of described fresh potato chips Degree is 2-3mm.

Compared with prior art, a technical scheme in technique scheme has the advantage that

A), in an embodiment of this utility model late blight of potato sampling apparatus, have employed openable sealing Box, is placed with fresh potato chips in seal box.After field uses, can immediately the late blight of potato disease sample sheared be placed on Under fresh potato chips, make phytophthora invade potato chips and breed, until producing mycelia.Decrease the pollution of disease sample, be conducive to quickly obtaining Obtain substantial amounts of bacterial strain.

B), in an embodiment of this utility model late blight of potato sampling apparatus, depression bar is big foot compression bar, it is possible to Prevent fresh potato chips to be punctured, damaged.

C) in an embodiment of this utility model late blight of potato sampling apparatus, described box body and described lid It is threaded connection, it is simple to seal, opens or closes seal box and be all not easy vibrations to fresh potato chips.

Accompanying drawing explanation

In order to be illustrated more clearly that the technical scheme of this utility model embodiment, below will be to required in embodiment The accompanying drawing used is briefly described, it will be appreciated that the following drawings illustrate only some embodiment of the present utility model, the most not Should be considered as the restriction to scope, for those of ordinary skill in the art, on the premise of not paying creative work, Other relevant accompanying drawings can also be obtained according to these accompanying drawings.

Fig. 1 is the exploded perspective structural representation of this utility model late blight of potato sampling apparatus one preferred embodiment.

Fig. 2 is the upward view of lid in this utility model late blight of potato sampling apparatus one preferred embodiment.

Fig. 3 is the structural representation of three kinds of depression bars in this utility model late blight of potato sampling apparatus one preferred embodiment Figure.

Figure acceptance of the bid note is respectively as follows:

1 box body,

11 external screw threads,

2 lids,

21 depression bars,

21a the first depression bar,

21b the second depression bar,

21c the 3rd depression bar,

3 fresh potato chips,

4 sick samples.

Detailed description of the invention

Illustrate with a specific embodiment below in conjunction with the accompanying drawings.

For making the purpose of this utility model embodiment, technical scheme and advantage clearer, below in conjunction with this practicality Accompanying drawing in novel embodiment, is clearly and completely described the technical scheme in this utility model embodiment, aobvious So, described embodiment is a part of embodiment of this utility model rather than whole embodiments.Based on this practicality Embodiment in novel, it is every other that those of ordinary skill in the art are obtained under not making creative work premise Embodiment, broadly falls into the scope of this utility model protection.Therefore, below to the enforcement of the present utility model provided in the accompanying drawings The detailed description of mode is not intended to limit claimed scope of the present utility model, but is merely representative of of the present utility model Selected embodiment.

It should also be noted that similar label and letter represent similar terms, therefore, the most a certain Xiang Yi in following accompanying drawing Individual accompanying drawing is defined, then it can not be defined further and explain in accompanying drawing subsequently.

See Fig. 1 to Fig. 3.Late blight of potato sampling apparatus described by the present embodiment, including open-topped box body 1, it is covered on the lid 2 of described opening, in box body 1, is placed with the fresh potato chips 3 for covering disease sample 4.In the present embodiment, box body 1 is separate with lid 2, and lid 2 can be sealed on box body 1, constitutes a seal box fully sealed.Certainly, box body 1 and lid Body 2 can also be connected with each other by connector.

The interior top of described lid 2 is provided with multiple depression bar 21, and the plurality of depression bar includes 2 and the depression bar of more than 2, this In embodiment, the quantity of depression bar 21 is 4.Contacting with described fresh potato chips 3 bottom described depression bar 21, the effect of depression bar is solid Fixed fresh potato chips 3 so that it is fixing.Fresh potato chips 3 are used for covering disease sample 4, when gathering sick sample, are first placed on by the sick sample sheared In box body 1, hit exactly it is further preferred that the sick sample sheared is placed on bottom box body 1, fresher potato chips 3 lid is placed on disease sample On, then cover lid, under the effect of depression bar, fresh potato chips 3 are also fixed, and sick sample 4 is also fixed.Fresh potato chips refer to just from The potato chips cut out in potato block, for infecting of late blight of potato phytophthora infestans.

The quantity that arranges of depression bar 21 is multiple, including 2 and more than 2, in the present embodiment, the depression bar 21 shown in Fig. 2 Quantity is 4, and 4 depression bars are distributed on foursquare four angles.Certainly, the quantity of depression bar can be 3,3 depression bars Lay respectively on three summits of an equilateral triangle.Although the quantity of the depression bar described in the present embodiment is multiple, but its quantity Also it is not intended to be infinite number of, it is possible to fresh potato chips are played suppression and is transporting late blight of potato sampling apparatus During fresh potato chips 3 will not rock and be advisable.

As selectable embodiment, the top of depression bar 21 is cambered surface, can reduce contacting of depression bar 21 and fresh potato chips Area.

As selectable embodiment, described depression bar 21 is big foot compression bar, and big foot compression bar includes a foot and bar portion, foot Portion's diameter is more than bar portion diameter, and cadre is fixed on the inner bottom of box body 1.Fig. 3 shows the situation of three kinds of big foot compression bars, the first pressure The foot of bar 21a is umbrella shape, and the foot of the second depression bar 21b is spherical, and the foot of the 3rd depression bar 21c is cylindrical.Big foot compression bar Foot's diameter more than the diameter of cadre, will not insert or damage fresh potato chips keeping depression bar.

The material of described box body 1 and described lid 2 is transparent plastic or glass, and the material of described depression bar 21 is silica gel, glass One in glass, plastics.As preferably, select to use silica gel material as depression bar, it is possible to protect fresh potato chips to be difficult to damaged. Depression bar 21 can also is that the elastomeric element that bottom is smooth, such as spring.

Described box body 1 and described lid 2 are circle, and described box body is threadeded with described lid.Fig. 1 shows box body Being provided with external screw thread 11 on 1, lid 2 is provided with the female thread screwed togather with described external screw thread 11, described female thread is in the accompanying drawings Not shown, but those skilled in the art just can know according to the general knowledge of this area.As preferably, fresh potato chips 3 are disk, when So, fresh potato chips 3 are not limited to disk, it is also possible to be that triangle, square etc. be regular or irregular polygon.

The internal diameter of described box body 1 is 5-10cm, and as preferably, the internal diameter of box body 1 is 5cm.Fresh potato chips the most smaller In 5cm, such as, the diameter of fresh potato chips 3 can select 4.5cm, the thickness of fresh potato chips 3 to be 2mm or 3mm, it is also possible to is 2- Any thickness in the range of 3mm.Susceptible variety, such as Favorita, river taro 56 etc. is selected for cutting the Rhizoma Solani tuber osi of fresh potato chips, First with 75% ethanol, potato ball is disinfected before section.

During the sampling of field, single scab disease leaf, clip 1-2cm first it are collected²Sick sample, is placed on sick sample in box body 1 Between, scene cuts out the potato block that thickness is 2-3mm on the potato ball sterilized, and potato block periphery is without Rhizoma Solani tuber osi Skin, lies in box body by fresh potato block, covers disease sample completely, covers lid, can take back use for laboratory in experiment. Under the conditions of 18-20 DEG C, cultivate 5-7 days, just can grow mycelia at potato block upper surface, just can be used for monangial bacterial strain Separate.

The present embodiment late blight of potato sampling apparatus is used to carry out the sampling of the late blight of potato in field, it is possible to avoid The pollution of miscellaneous bacteria, it is to avoid directly sick belt transect is returned the destruction to leaf surface bacterium colony during laboratory.

Use the mode of buckle to connect as selectable embodiment, box body 1 and lid 2, use the mode of snapping even When connecing, box body 1 can not be circular with lid 2, such as oval, square, rectangle.

In description of the present utility model, it is to be understood that term " " center ", " thickness ", " on ", D score, " water Flat ", the orientation that indicates of " top ", " end ", " interior ", " outward " etc. or position relationship be based on orientation shown in the drawings or position relationship, It is for only for ease of description this utility model and simplifies description rather than instruction or imply that the equipment of indication or element must have Specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to restriction of the present utility model.

Additionally, term " first ", " second " are only used for describing purpose, and it is not intended that instruction or hint relative importance Or the implicit quantity indicating indicated technical characteristic.Thus, define " first ", the feature of " second " can express or Implicitly include one or more this feature.In description of the present utility model, " multiple " are meant that two or two Above, unless otherwise expressly limited specifically.

In this utility model, unless otherwise clearly defined and limited, fisrt feature second feature it " on " or it D score can include that the first and second features directly contact, it is also possible to includes that the first and second features are not directly contact but logical Cross the other characterisation contact between them.And, fisrt feature second feature " on ", " top " and " above " include One feature is directly over second feature and oblique upper, or is merely representative of fisrt feature level height higher than second feature.First is special Levy second feature " under ", " lower section " and " below " include fisrt feature immediately below second feature and obliquely downward, or only Represent that fisrt feature level height is less than second feature.

Below it is only preferred implementation of the present utility model, it is noted that above-mentioned preferred implementation should not regard For to restriction of the present utility model, protection domain of the present utility model should be as the criterion with claim limited range.For For those skilled in the art, without departing from spirit and scope of the present utility model, it is also possible to make some Improvements and modifications, these improvements and modifications also should be regarded as protection domain of the present utility model.

Claims (8)

1. a late blight of potato sampling apparatus, it is characterised in that include open-topped box body (1), be covered on described in open The lid (2) of mouth, is placed with the fresh potato chips (3) for covering disease sample (4) in box body (1), the interior top of described lid (2) is installed Multiple depression bar (21), described depression bar (21) bottom is had to contact with described fresh potato chips (3).

Late blight of potato sampling apparatus the most according to claim 1, it is characterised in that the top of described depression bar (21) is Cambered surface.

Late blight of potato sampling apparatus the most according to claim 1 and 2, it is characterised in that described depression bar (21) is big Angle depression bar.

Late blight of potato sampling apparatus the most according to claim 1 and 2, it is characterised in that the material of described depression bar (21) Matter is the one in silica gel, glass, plastics.

Late blight of potato sampling apparatus the most according to claim 1, it is characterised in that described box body (1) and described lid Body (2) is circle, and described box body is threadeded with described lid.

Late blight of potato sampling apparatus the most according to claim 5, it is characterised in that the internal diameter of described box body (1) is 5-10cm。

Late blight of potato sampling apparatus the most according to claim 5, it is characterised in that described fresh potato chips (3) are circle Sheet.

Late blight of potato sampling apparatus the most according to claim 1, it is characterised in that the thickness of described fresh potato chips (3) Degree is 2-3mm.