CN205774465U

CN205774465U - A kind of vacuum nucleic acid extraction column for extracting cfDNA

Info

Publication number: CN205774465U
Application number: CN201620496838.9U
Authority: CN
Inventors: 疏义林; 万琴; 周昇; 张守德
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-05-27
Filing date: 2016-05-27
Publication date: 2016-12-07
Anticipated expiration: 2026-05-27

Abstract

This utility model belongs to the field of nucleic acid purification in biotechnology, is specifically related to a kind of vacuum nucleic acid extraction column for extracting cfDNA, including extracting inner prop, extracting column jacket, cross over pipe；Described extraction column jacket includes upper prop, lower prop, described upper column diameter is more than lower column diameter, and upper prop and lower prop are connected as the integrative-structure of UNICOM, are provided with upper filter screen and lower filter screen in described lower prop, upper filter screen is positioned at lower prop top, filling glass pearl between upper filter screen and upper prop bottom；This utility model production efficiency is high, and product purity is high, the easy to operate suitable popularization and application of simple in construction.

Description

A kind of vacuum nucleic acid extraction column for extracting cfDNA

Technical field

This utility model belongs to the field of nucleic acid purification in biotechnology, be specifically related to a kind of for Extract the vacuum nucleic acid extraction column of cfDNA.

Background technology

No matter the existing method extracting nucleic acid by medium, mainly have centrifugal column method and paramagnetic particle method Which kind of method, principle is all the characteristic of silicon oxide substrate absorption nucleic acid.Centrifugal column method is used to extract Nucleic acid, is affected by silicagel column volume, it is difficult to extract the sample more than 200 μ L.And use magnetic bead Method, although magnetic bead itself can infinitely increase, but often by absorbability and the magnetic bead of Magnet Can remaining a small amount of magnetic bead in the impact of adsorbable area, and the nucleic acid finally often extracted.

CfDNA is DNA free in blood, and often content is low, needs to carry out substantial amounts of sample Enrichment is extracted, and can be only achieved the requirement of downstream experiment detection.Traditional centrifugal column or paramagnetic particle method, All it is difficult to reach this requirement.

Utility model content

Little in order to solve prior art is extracted the method extracted amount of nucleic acid, extract in product and exist The defects such as impurity, this utility model provides a kind of production efficiency high, and product purity is high, structure letter A kind of vacuum nucleic acid extraction column for extracting cfDNA of single operation.

It is as follows that this utility model solves the technical scheme that its technical problem used: one is used for carrying Take the vacuum nucleic acid extraction column of cfDNA, including extracting inner prop, extracting column jacket, cross over pipe；Described Extracting column jacket and include upper prop, lower prop, described upper column diameter is more than lower column diameter, upper prop and lower prop Being connected as the integrative-structure of UNICOM, be provided with upper filter screen in described lower prop, upper filter screen is positioned on lower prop Portion, filling glass pearl between upper filter screen and upper prop bottom, lower prop bottom end opening；In described extraction Post external diameter is less than or equal to upper column internal diameter, is nested in upper prop；Described cross over pipe is T-shaped pipe, point For vertical tube and transverse tube, transverse tube is positioned at riser middle, and riser upper end is connected with lower prop bottom, transverse tube It is connected with air pump, vertical tube lower end base opening.

Also include that lower filter screen, described lower filter screen are positioned at lower prop bottom end opening.

Described extraction inner prop includes extracting inner wall and extracting inner prop filter screen, extracts inner prop filter screen position In extracting inner wall bottom.

Also include that fixing lug boss, described fixing lug boss are positioned at extraction inner prop top outer wall, or fixing Boss is positioned in the middle part of upper prop, and the length of fixing lug boss to upper prop top is equal to column length in extracting； The fixed extraction inner prop position when extracting inner prop and being nested in upper prop.

Described cross over pipe includes outer tube and silylation layer, and silylation layer is attached to outer tube wall.

Being provided with connected unit at described cross over pipe bottom end opening, described connected unit outer wall inwall is equipped with Screw thread, also includes that attachment cap, described attachment cap medial wall are provided with screw thread, with connected unit screw thread even Connect, seal.

Described connected unit can also and the equipment such as centrifuge tube be attached, to facilitate extraction work.

Breathing waterproof film it is provided with between transverse tube and air pump in the middle part of described cross over pipe.

Described breathing waterproof film is positioned at the position that cross over pipe vertical tube is connected with transverse tube.

The beneficial effects of the utility model are:

This utility model may be used for the sample extraction of the large volume low concentrations such as cfDNA, compares general Centrifugal column extracting method, the method extract nucleic acid can concentrate 7.5 times；So that effect Rate and yield and product quality all get a promotion.

Save extraction spent time；Extraction column is recyclable.This utility model scientific structure closes Reason, easily manufactured, suitable popularization and application.

Accompanying drawing explanation

Fig. 1 is embodiment 1 structural representation；

Fig. 2 is embodiment 1 partial resolution structural representation；

Fig. 3 is that embodiment 2 extracts inner prop structural representation；

Fig. 4 is embodiment 3 structural representation.

Detailed description of the invention

Describe embodiment of the present utility model with reference to the accompanying drawings in detail.

Embodiment 1:

See Fig. 1-2 the present embodiment structural representation:

A kind of vacuum nucleic acid extraction column for extracting cfDNA, outside extracting inner prop 1, extracting Post 2, cross over pipe 3；Described extraction column jacket 2 includes upper prop 4, lower prop 5, and described upper prop 4 diameter is big It is connected as the integrative-structure of UNICOM in lower prop 5 diameter, upper prop 4 and lower prop 5, sets in described lower prop 5 Having upper filter screen 6 and lower filter screen 7, upper filter screen 6 is positioned at lower prop 5 top, upper filter screen 6 and upper prop 4 bottom Between filling glass pearl 12, lower prop 5 bottom end opening, described lower filter screen 7 is positioned at lower prop 5 bottom end opening Place；Described extraction inner prop external diameter is less than or equal to upper prop 4 internal diameter, is nested in upper prop 4；Described Cross over pipe 3 is T-shaped pipe, is divided into vertical tube 9 and transverse tube 10, and transverse tube 10 is positioned in the middle part of vertical tube 9, vertical tube 9 Upper end is connected with lower prop 5 bottom, and transverse tube 10 is connected with air pump 20, vertical tube 9 lower end base opening.

Described extraction inner prop 1 includes extracting inner wall 11 and extracting inner prop filter screen 8, extracts inner prop filter Net 8 is positioned at extraction inner wall 11 bottom.

Also include that fixing lug boss 13, described fixing lug boss 13 are positioned in the middle part of upper prop 4, fixing lug boss 13 Length to upper prop 4 top is equal to extract inner prop 1 length；It is nested in upper prop 4 extracting inner prop 1 Time fixed extraction inner prop 1 position.

Described cross over pipe includes outer tube 14 and silylation layer 15, and silylation layer 15 is attached to outer tube 14 inwall.

Being provided with connected unit 16 at described cross over pipe bottom end opening, described connected unit 16 outer wall inwall is equal It is provided with screw thread, also includes that attachment cap 17, described attachment cap 17 medial wall are provided with screw thread, and be connected Platform 16 is threaded, seals.

Breathing waterproof film 18 it is provided with between transverse tube 10 and air pump in the middle part of described cross over pipe.

Described breathing waterproof film 18 is positioned at the position that cross over pipe vertical tube 9 is connected with transverse tube 10.

Embodiment 2:

With reference to Fig. 3, fixing lug boss 13 is positioned at extraction inner prop 1 top outer wall so that extract inner prop 1 embedding Fixed extraction inner prop 1 position when being enclosed within upper prop 4.Remaining is with embodiment 1.

Embodiment 3:

With reference to Fig. 4, described connected unit 16 and centrifuge tube equipment are attached, to facilitate extraction work, Remaining is with embodiment 1.

Certainly, the above is preferred implementation of the present utility model, it is noted that for For those skilled in the art, on the premise of without departing from this utility model principle, Can also make some improvements and modifications, these improvements and modifications are also considered as guarantor of the present utility model Protect scope.

Claims

1. the vacuum nucleic acid extraction column being used for extracting cfDNA, it is characterised in that: include carrying Take inner prop, extract column jacket, cross over pipe；Described extraction column jacket includes upper prop, lower prop, described on Column diameter is more than lower column diameter, and upper prop and lower prop are connected as the integrative-structure of UNICOM, described lower prop Inside being provided with upper filter screen, upper filter screen is positioned at lower prop top, fills glass between upper filter screen and upper prop bottom Glass pearl, lower prop bottom end opening；Described extraction inner prop external diameter is less than or equal to upper column internal diameter, nested In upper prop；Described cross over pipe is T-shaped pipe, is divided into vertical tube and transverse tube, and transverse tube is positioned at riser middle, Riser upper end is connected with lower prop bottom, and transverse tube is connected with air pump, vertical tube lower end base opening.

A kind of vacuum nucleic acid extraction column for extracting cfDNA the most according to claim 1, It is characterized in that: also include that lower filter screen, described lower filter screen are positioned at lower prop bottom end opening；Described Extract inner prop to include extracting inner wall and extracting inner prop filter screen, extract inner prop filter screen and be positioned at extraction Post jamb bottom.

A kind of vacuum nucleic acid extraction column for extracting cfDNA the most according to claim 2, Also include that fixing lug boss, described fixing lug boss are positioned at extraction inner prop top outer wall, or fixing lug boss Being positioned in the middle part of upper prop, the length of fixing lug boss to upper prop top is equal to column length in extracting；Carrying Take fixed extraction inner prop position when inner prop is nested in upper prop.

A kind of vacuum nucleic acid extraction column for extracting cfDNA the most according to claim 1, Described cross over pipe includes outer tube and silylation layer, and silylation layer is attached to outer tube wall.

A kind of vacuum nucleic acid extraction column for extracting cfDNA the most according to claim 1, Being provided with connected unit at described cross over pipe bottom end opening, described connected unit outer wall inwall is equipped with screw thread, Also include that attachment cap, described attachment cap medial wall are provided with screw thread, threaded with connected unit, enter Row seals.

A kind of vacuum nucleic acid extraction column for extracting cfDNA the most according to claim 1, Breathing waterproof film it is provided with between transverse tube and air pump in the middle part of described cross over pipe.

A kind of vacuum nucleic acid extraction column for extracting cfDNA the most according to claim 6, Described breathing waterproof film is positioned at the position that cross over pipe vertical tube is connected with transverse tube.