CN205695269U - A kind of Dual-closed type ovum or embryo vitrifying freeze save set - Google Patents
A kind of Dual-closed type ovum or embryo vitrifying freeze save set Download PDFInfo
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- CN205695269U CN205695269U CN201620353840.0U CN201620353840U CN205695269U CN 205695269 U CN205695269 U CN 205695269U CN 201620353840 U CN201620353840 U CN 201620353840U CN 205695269 U CN205695269 U CN 205695269U
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Abstract
The utility model relates to a kind of Dual-closed type ovum or embryo vitrifying freeze save set, it is characterized in that: include closed outer tube (12) and closed inner tube (5), closed inner tube (5) is provided with interior tube chamber (1) and inner filtration system (6), closed outer tube (12) is provided with outer lumen (10) and outer filtration system (13), closed inner tube (5) is positioned in outer lumen (10), ovum or embryo (3) are placed in interior tube chamber (1), outside liquid nitrogen passes sequentially through outer filtration system (13), after inner filtration system (6) is degerming, ovum or embryo (3) are carried out freezing.The utility model takes full advantage of the mode of liquid nitrogen aseptic filtration system and multiple times of filtration, substantially increases the security of ovum and embryo cryopreservation.
Description
Technical field
The utility model relates to Med Biol Eng technical field, is specifically related to a kind of double-enclosure
Formula ovum or embryo vitrifying freeze save set, it utilizes aseptic filtration system, is used for aseptic
Cryogenic freezing preserves ovum or embryo.
Background technology
The freezen protective of the ovum of the mankind and embryo has been in Issues of Human Assisted Reproductive Technologies field one
Individual pith.In past these 20 years, the development of freezen protective technology also can reflect the mankind
The development degree of auxiliary procreation technology, present Refrigeration Technique can be used on freezen protective ovum and
The embryo of different times, especially vitrification, replaced the slow art freezing of tradition,
And become the optimal selection of Refrigeration Technique, even by this Technology application at highly difficult ovum, ovum
In the freezing of nest tissue and hESC.
Either egg freezing or Embryo freezing preservation, is required for a physical support, generally
Described load bar or straw etc..The ovum of current Reproductive Medicine Center employing or embryo cryopreservation system,
Mostly be exploitation formula as carried bar, i.e. embryo is directly exposed in the common liquid nitrogen of filtered so that
Ovum or embryo may be caused potential pollution by the pathogen in liquid nitrogen.Such as open freezing load
Body (cryotop), freezing ring (cryoloop), open type glass microcapillary (GMP),
Open elongation straw (OPS), electron microscope copper mesh etc..The freezing carrier of these types,
The freezing load bar using such as KITAZATO company of Japan, in its pipe and is the liquid of pollution outside pipe
Nitrogen, the solution containing embryo is directly exposed in liquid nitrogen, and the method can not be completely eliminated
Cross-infection risk during embryo freezing.Even if there being part producer design and produce semiclosed
The refrigerating plant of formula, but do not considered that liquid nitrogen filtered, simply ovum or embryo are inserted an envelope
In the environment closing, completely cut off embryo and the contacting of other liquid nitrogen containing noxious material in liquid nitrogen container, added
Putting on airs JY company, the liquid nitrogen in this cryovial remains to exist and pollutes.
Utility model content
The utility model devises a kind of Dual-closed type ovum or embryo vitrifying freeze save set,
Its solve the technical problem that be current Reproductive Medicine Center use ovum or embryo cryopreservation system many
For exploitation formula, i.e. embryo is directly exposed in the common liquid nitrogen of filtered so that in liquid nitrogen
Ovum or embryo may be caused potential pollution by pathogen.
In order to solve the technical problem of above-mentioned existence, the utility model have employed below scheme:
A kind of Dual-closed type ovum or embryo vitrifying freeze save set, it is characterised in that: bag
Including closed outer tube (12) and closed inner tube (5), closed inner tube (5) is provided with inner tube
Chamber (1) and inner filtration system (6), closed outer tube (12) be provided with outer lumen (10) and
Outer filtration system (13), closed inner tube (5) is positioned in outer lumen (10), ovum or embryo
Tire (3) is placed in interior tube chamber (1), outside liquid nitrogen pass sequentially through outer filtration system (13),
After inner filtration system (6) is degerming, ovum or embryo (3) are carried out freezing.
Further, closed inner tube (5) one end carries out airtight by containing bar (4), contains
Bar (4) is provided with ovum or embryo's set-point (2), and closed inner tube (5) other end passes through
Interior quoit (7) carries out airtight, and interior quoit (7) inner side is provided with inner filtration system (6),
Interior tube chamber liquid nitrogen entrance (9) is had, tube chamber liquid nitrogen entrance (9) in entering on interior quoit (7)
Liquid nitrogen by eventually entering in interior tube chamber (1) after inner filtration system (6).
Further, described to contain bar (4) one end relatively thin, and the other end is thicker；Ovum or embryo are put
Put point (2) to be positioned on thinner end, carry out, compared with butt end and inner sleeve open-ended (8), the company of sealing
Connect.
Further, described inner filtration system (6) is sterilised membrane filter post or aseptic filter post.
Further, closed outer tube (12) one end carries out airtight by outer rockbolt (11), close
Enclosed outer tube (12) other end carries out airtight by outer quoit (14), outer quoit (14)
Upper inner side is provided with outer filtration system (13), outer quoit (14) has outer lumen liquid nitrogen and enters
Mouth (16), enters the liquid nitrogen of outer lumen liquid nitrogen entrance (16) by outer filtration system (13)
After eventually enter in outer lumen (10).
Further, described outer filtration system (13) is sterilised membrane filter post or aseptic filter post.
Further, described ovum or embryonic origin are in mammal.
Further, described mammal behaviour, mouse, rat, rabbit, sheep, dog, cat or ox.
This Dual-closed type ovum or embryo vitrifying freeze save set have the advantages that
(1) the utility model takes full advantage of the side of liquid nitrogen aseptic filtration system and multiple times of filtration
Formula, substantially increases the security of ovum and embryo cryopreservation.
(2) the utility model utilizes filtration system to filter liquid nitrogen, makes ovum or embryo be in aseptic
In the environment preserving, and the temperature rate during not affecting glass freezing, greatly reduce
The possibility polluted during ovum or Embryo freezing preservation.
Brief description
Fig. 1: the utility model Dual-closed type ovum or embryo vitrifying freeze save set structure are shown
It is intended to.
Description of reference numerals:
Tube chamber in 1-；2-ovum or embryo's set-point；3-ovum or embryo；4-contains bar；
5-closed inner tube；6-inner filtration system；Quoit in 7-；8-inner sleeve is open-ended；
Tube chamber liquid nitrogen entrance in 9-；10-outer lumen；The outer rockbolt of 11-；12-closed outer tube；13
-outer filtration system；The outer quoit of 14-；15-outer tube is open-ended；16-outer lumen liquid
Nitrogen entrance.
Detailed description of the invention
Below in conjunction with Fig. 1, the utility model is described further:
As it is shown in figure 1, a kind of Dual-closed type ovum described in the utility model or embryo vitrifying are cold
Freeze save set, be to utilize aseptic filtration system, be used for cryogenic sterile freezen protective ovum or embryo
Tire.
A kind of Dual-closed type ovum or embryo vitrifying freeze save set, including closed outer tube
12 and closed inner tube 5, closed outer tube 12 and closed inner tube 5 all have filter membrane or filter post,
With guarantee liquid nitrogen aseptic filtration laggard enter official jargon.
Closed inner tube 5 includes containing bar 4 and interior tube chamber 1.Containing bar 4 is entity, front thick after
Carefully, ovum or embryo's set-point 2 are contained in the rear end attenuating.Closed inner tube 5 is pipe-like, front
End opening, its end contains bolt (metal inner ring) and sterilised membrane filter (aseptic filter post) in metal,
Containing tube chamber 1 in inserting after bar 4 loads embryo, both combine formation closed inner tube 5.Insert again
After entering liquid nitrogen, tube chamber supper-fast cooling formed negative pressure state, liquid nitrogen by negative pressure through inner tube
Sterilised membrane filter or aseptic filter post rapidly enter interior tube chamber 1, with ensure enter liquid nitrogen as germ-free condition,
Promote embry ogenesis glass freezing simultaneously.
Closed outer tube 12 volume is thick compared with closed inner tube, can accommodate closed inner tube 5 and insert
In it.Closed outer tube 12 includes outer rockbolt 11 and outer lumen 10, and outer lumen 10 end is containing gold
Bolt (metal inner ring) and filter membrane (filter post) in belonging to, outer rockbolt 11 inserts both outer lumen 10
In conjunction with making tube chamber be sealed shape, after liquid nitrogen inserted by closed outer tube 12, liquid nitrogen is only capable of by filter
Film (filter post) passes in and out outer lumen 10, it is ensured that the germ-free condition of airtight outer lumen 10.
Ovum or embryonic origin in mammal, including people, mouse, rat, rabbit, sheep, dog,
Cat, ox etc..
Specific embodiment, Preservation in sterile condition technology of the present utility model is preferable over open or semiclosed
Formula freezing storage device:
Embodiment 1:
1st, gamma ray sterilizing method pair will be utilized, to the utility model Dual-closed type ovum or embryo
Tire vitrificated cryopreserration device sterilizes；
2nd, it is poured onto liquid nitrogen in one small container；
3rd, closed outer tube 12 is inserted in liquid nitrogen, keep outer tube opening part and outer rockbolt 11
It is all located at outside liquid nitrogen, and keep skewed；
4th, after 3 minutes, treat that liquid nitrogen enters outer lumen 10 through the outer filtration system 13 below outer tube,
Extract outer rockbolt 11；
5th, insert closed inner tube 5 in closed outer tube 12；
6th, after 3 minutes, liquid nitrogen enters inner tube through the inner filtration system 6 below closed inner tube 5
Chamber 1；
7th, utilize aseptic nipper to go out interior pipe clamp, be placed into bacteria culture media, 37 degrees Celsius of cultivations
48 hours, observation bacterial growth situation:
8th, business-like open and semi-enclosed refrigerating plant is processed, i.e. directly equally
It is exposed in liquid nitrogen, so insert bacteria culture media and carry out Bacteria Culture；
9th, result is as follows:
Embodiment 2:
1st, embryo vitrifying freeze liquid is taken out from refrigerator, be placed in equilibrium at room temperature 5 minutes；
2nd, in plate, freezing liquid ES liquid droplet and VS liquid droplet are made；
3rd, the closed outer tube 12 of double-enclosure system glass refrigerating plant is inserted in liquid nitrogen flat
Weighing apparatus；
4th, embryo is transferred to from culture medium in ES liquid balance 5 minutes；
5th, again cultivation is transferred to from ES liquid in VS liquid 30 seconds；
6th, embryo is transferred quickly to Dual-closed type ovum or embryo vitrifying freeze save set
Embryo's set-point of closed inner tube 5；
7th, closed inner tube 5 carry out airtight after, put into the closed outer tube 12 in liquid nitrogen；
8th, outer rockbolt 11 is closed closed outer tube 12；
9th, whole device is transferred to the medium-term and long-term preservation of liquid nitrogen.
Above in conjunction with accompanying drawing, exemplary description is carried out to the utility model, it is clear that this practicality is new
The realization of type is not subject to the restrictions described above, as long as have employed method of the present utility model design
The various improvement carrying out with technical scheme, or not improved by design of the present utility model and technology
Scheme directly applies to other occasions, all in protection domain of the present utility model.
Claims (10)
1. a Dual-closed type ovum or embryo vitrifying freeze save set, it is characterised in that:
Including closed outer tube (12) and closed inner tube (5), in closed inner tube (5) is provided with
Tube chamber (1) and inner filtration system (6), closed outer tube (12) is provided with outer lumen (10)
With outer filtration system (13), closed inner tube (5) is positioned in outer lumen (10), ovum or
Embryo (3) is placed in interior tube chamber (1), outside liquid nitrogen pass sequentially through outer filtration system (13),
After inner filtration system (6) is degerming, ovum or embryo (3) are carried out freezing.
2. Dual-closed type ovum or embryo vitrifying freeze save set according to claim 1,
It is characterized in that: closed inner tube (5) one end carries out airtight by containing bar (4), contains
Bar (4) is provided with ovum or embryo's set-point (2), and closed inner tube (5) other end passes through
Interior quoit (7) carries out airtight, and interior quoit (7) inner side is provided with inner filtration system (6),
Interior tube chamber liquid nitrogen entrance (9) is had, tube chamber liquid nitrogen entrance (9) in entering on interior quoit (7)
Liquid nitrogen by eventually entering in interior tube chamber (1) after inner filtration system (6).
3. Dual-closed type ovum or embryo vitrifying freeze save set according to claim 2,
It is characterized in that: described to contain bar (4) one end relatively thin, and the other end is thicker；Ovum or embryo are put
Put point (2) to be positioned on thinner end, carry out, compared with butt end and inner sleeve open-ended (8), the company of sealing
Connect.
4. according to Claims 2 or 3, Dual-closed type ovum or embryo vitrifying freeze preserve
Device, it is characterised in that: described inner filtration system (6) is sterilised membrane filter post or aseptic filter post.
5. Dual-closed type ovum or embryo vitrifying freeze save set according to claim 4,
It is characterized in that: closed outer tube (12) one end carries out airtight by outer rockbolt (11), close
Enclosed outer tube (12) other end carries out airtight by outer quoit (14), outer quoit (14)
Upper inner side is provided with outer filtration system (13), outer quoit (14) has outer lumen liquid nitrogen and enters
Mouth (16), enters the liquid nitrogen of outer lumen liquid nitrogen entrance (16) by outer filtration system (13)
After eventually enter in outer lumen (10).
6. Dual-closed type ovum or embryo vitrifying freeze save set according to claim 5,
It is characterized in that: described outer filtration system (13) is sterilised membrane filter post or aseptic filter post.
7. Dual-closed type ovum or embryo's glass according to claim the 1st, the 2nd, the 3rd, 5 or 6
Change freezing storage device, it is characterised in that: described ovum or embryonic origin are in mammal.
8. Dual-closed type ovum or embryo vitrifying freeze save set according to claim 4,
It is characterized in that: described ovum or embryonic origin are in mammal.
9. Dual-closed type ovum or embryo vitrifying freeze save set according to claim 7,
It is characterized in that: described mammal behaviour, mouse, rat, rabbit, sheep, dog, cat or ox.
10. Dual-closed type ovum or embryo vitrifying freeze preserve dress according to claim 8
Put, it is characterised in that: described mammal behaviour, mouse, rat, rabbit, sheep, dog, cat
Or ox.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620353840.0U CN205695269U (en) | 2016-04-26 | 2016-04-26 | A kind of Dual-closed type ovum or embryo vitrifying freeze save set |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620353840.0U CN205695269U (en) | 2016-04-26 | 2016-04-26 | A kind of Dual-closed type ovum or embryo vitrifying freeze save set |
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| CN205695269U true CN205695269U (en) | 2016-11-23 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372470A (en) * | 2017-09-11 | 2017-11-24 | 马博 | A kind of ovum embryo vitrifying freeze save set |
| CN108496956A (en) * | 2018-03-30 | 2018-09-07 | 浙江大学 | A kind of freezing of human embryos carries auxiliary security docking facilities and its application method under bar liquid nitrogen |
| WO2020147604A1 (en) * | 2019-01-18 | 2020-07-23 | 上海廪典实业有限公司 | Ultralow-temperature freezing and sealing type preservation method |
| CN115486437A (en) * | 2021-06-18 | 2022-12-20 | 上海明悦医疗科技有限公司 | Auxiliary reproduction carrier bar assembly |
-
2016
- 2016-04-26 CN CN201620353840.0U patent/CN205695269U/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372470A (en) * | 2017-09-11 | 2017-11-24 | 马博 | A kind of ovum embryo vitrifying freeze save set |
| CN107372470B (en) * | 2017-09-11 | 2021-05-28 | 马博 | Ovum embryo vitrification cryopreservation device |
| CN108496956A (en) * | 2018-03-30 | 2018-09-07 | 浙江大学 | A kind of freezing of human embryos carries auxiliary security docking facilities and its application method under bar liquid nitrogen |
| CN108496956B (en) * | 2018-03-30 | 2023-09-01 | 浙江大学 | Auxiliary safety docking device under liquid nitrogen of human embryo freezing carrier rod and application method of auxiliary safety docking device |
| WO2020147604A1 (en) * | 2019-01-18 | 2020-07-23 | 上海廪典实业有限公司 | Ultralow-temperature freezing and sealing type preservation method |
| CN115486437A (en) * | 2021-06-18 | 2022-12-20 | 上海明悦医疗科技有限公司 | Auxiliary reproduction carrier bar assembly |
| CN115486437B (en) * | 2021-06-18 | 2024-01-23 | 上海明悦医疗科技有限公司 | Auxiliary reproduction carrying rod assembly |
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