CN205603587U

CN205603587U - DNA chip

Info

Publication number: CN205603587U
Application number: CN201620165789.0U
Authority: CN
Inventors: 邢红兵
Original assignee: Jiangsu Huachuang Biological Technology Co Ltd
Current assignee: Jiangsu Huachuang Biological Technology Co Ltd
Priority date: 2016-03-04
Filing date: 2016-03-04
Publication date: 2016-09-28
Anticipated expiration: 2026-03-04

Abstract

The utility model discloses a DNA chip, including temperature controlled plate, establish solid phase carrier in temperature controlled plate, establish the detecting area on solid phase carrier, establish the probe matrix on the detecting area, temperature controlled plate on establish the deep trouth, through heat -conducting glue bonding solid phase carrier in the deep trouth. The utility model discloses the DNA chip of heat establishes solid phase carrier in temperature controlled plate to through heat -conducting glue bonding solid phase carrier, be favorable to solid phase carrier's heat transfer, guarantee the service property (quality), establish different probes and put to the proof on solid phase carrier, both can realize functions such as gene expression register for easy reference survey, mutation detection, polymorphism analysis, have fine practicality.

Description

A kind of DNA Chip

Technical field

This utility model belongs to gene engineering technology field, is specifically related to a kind of DNA chip.

Background technology

Along with progressively implementing and the fast development of molecular biology related discipline of the Human Genome Project, increasing animals and plants, microbial genome sequence are measured, and the most former speed not having of gene sequence data increases rapidly.But how to go to study the function that such numerous gene undertaken in life process and just become the common problem of whole world life sciences worker.To this end, set up novel hybridization and sequence measurement with substantial amounts of hereditary information is carried out detection efficient, quick, analyze just seem increasingly important.

Biochip technology is exactly to comply with the product of this scientific development requirement, and its solution problems that appears as provides the prospect of radiance.This technology means that a large amount of probe molecules are fixed on holder the rear sample molecule with labelling to be hybridized, by detecting the hybridization signal intensities of each probe molecule and then obtaining quantity and the sequence information of sample molecule.Generally, it is simply that by micro-processing technology, by the ten hundreds of or even DNA fragmentation of million particular sequences counted, 2cm is fixed in arrangement regularly²Silicon chip, on the holder such as slide, a two-dimentional DNA probe array of composition, quite similar with the electronic chip of computer, so being referred to as gene chip.Gene chip is mainly used in gene test work.As far back as the eighties, short DNA segment is just fixed on holder by Bains W. et al., carries out sequencing by Crossing system.But it is directly to have benefited from probe solid phase situ synthesis techniques and the combination of Photolithographic techniques and the introducing of laser scanning confocal microscopy that gene chip moves towards the industrialization from laboratory.It makes synthesis, fixing highdensity ten hundreds of probe molecule practical, and by the micro-scanning technique of laser co-focusing allow to hybridization signal is carried out real-time, sensitive, detect and analyze accurately.As vacuum tube circuit is as transistor circuit and integrated circuit development are to be experienced, and the integrated of nucleic acid hybridization technique the most and has made Protocols in Molecular Biology that one revolution occurs.The existing company more than ten in the whole world specializes in the research-and-development activity of gene chip now, and the product of existing more molding and equipment come out.Main representative is Affymetrix company of the U.S..The many bit machines of the said firm's populated with, mathematics and molecular biology expert, its annual reasearch funds are more than 10,000,000 dollars, and lasted 6 or seven years as long as, have multinomial patent.Product or will have part and puts on market, and the Social benefit and economic benefit of generation makes us looking forward or upwards mesh.

Biochip technology is owing to being fixed on a large amount of probes on holder simultaneously, so can disposably sequences a large amount of to sample be detected and analyzed, thus solve the deficiencies such as tradition nucleic acid blot hybridization technique trivial operations, automaticity is low, sequence of operation quantity is few, detection efficiency is low.And, by the different probe array of design, use specific analysis method that this technology can be made to have multiple different using value, as gene expression profile mensurations, abrupt climatic change, polymorphism analysis, genomic library are mapped and sequencing by hybridization etc..

Utility model content

Sending out item: for the deficiencies in the prior art, the purpose of this utility model is to provide a kind of DNA chip, meets the use demands such as gene expression profile mensuration, abrupt climatic change, polymorphism analysis.

Technical scheme: for achieving the above object, the technical solution adopted in the utility model is as follows:

A kind of DNA chip, including temperature control plate, sets solid phase carrier in temperature control plate, sets detecting area on solid phase carrier, set probe matrix on detecting area；Described temperature control plate sets deep trouth, is bondd solid phase carrier by heat-conducting glue in deep trouth.

Described temperature control plate is metallic plate, macromolecular material plate or composite plate.

The upper surface of described solid phase carrier is equal with the upper surface of temperature control plate.

Described solid phase carrier is microscope slide.Fabricated in situ or micro-printing type solidification probe matrix on described microscope slide.

Beneficial effect: compared with prior art, the DNA chip of this utility model heat, solid phase carrier is set in temperature control plate 1, and bondd solid phase carrier by heat-conducting glue, be conducive to the heat transfer of solid phase carrier, it is ensured that use quality, solid phase carrier set different probes and puts to the proof, both can realize the functions such as gene expression profile mensuration, abrupt climatic change, polymorphism analysis, there is good practicality.

Accompanying drawing explanation

Fig. 1 is the structural representation of DNA chip.

Detailed description of the invention

Below in conjunction with concrete accompanying drawing, this utility model is described further.

As it is shown in figure 1, DNA chip, including temperature control plate 1, in temperature control plate 1, set solid phase carrier 2, on solid phase carrier 2, set detecting area 4, detecting area 3 sets probe matrix.

Temperature control plate 1 is the plate being prone to heat conduction, can be metallic plate, macromolecular material plate, and composite board, excellent thermal conductivity, such as copper coin.Setting deep trouth on temperature control plate 1, bondd solid phase carrier 2 by heat-conducting glue 3 in groove, the upper surface of solid phase carrier 2 is equal with the upper surface of temperature control plate 1.Solid phase carrier 2 can be microscope slide, fabricated in situ or micro-printing type solidification probe matrix on microscope slide.

DNA chip of the present utility model, solid phase carrier is set in temperature control plate 1, and bondd solid phase carrier by heat-conducting glue, be conducive to the heat transfer of solid phase carrier, ensure to use quality, solid phase carrier sets different probes put to the proof, both can realize the functions such as gene expression profile mensuration, abrupt climatic change, polymorphism analysis, there is good practicality.

Claims

1. a DNA chip, it is characterised in that include temperature control plate (1), sets solid phase carrier (2) in temperature control plate (1), sets detecting area (4) on solid phase carrier (2), set probe matrix on detecting area (4)；Described temperature control plate (1) sets deep trouth, by heat-conducting glue (3) bonding solid phase carrier (2) in deep trouth.

DNA chip the most according to claim 1, it is characterised in that: described temperature control plate (1) is metallic plate, macromolecular material plate or composite plate.

DNA chip the most according to claim 1, it is characterised in that: the upper surface of described solid phase carrier (2) is equal with the upper surface of temperature control plate (1).

DNA chip the most according to claim 1, it is characterised in that: described solid phase carrier (2) is microscope slide.

DNA chip the most according to claim 4, it is characterised in that: fabricated in situ or micro-printing type solidification probe matrix on described microscope slide.