CN205511406U - Upper and lower open -ended plant tissue culture bottle - Google Patents

Abstract

The utility model discloses an upper and lower open -ended plant tissue culture bottle belongs to plant tissue culture technical field, including the transparent bottle, the bottle upper end is provided with the bottleneck, and upward bottle lid is connected to the bottleneck, goes up the bottle lid and is provided with the air vent, the bottle bottom sets up the under shed, is provided with down the bottle lid on the under shed, is provided with the grid on the lower bottle lid. The utility model discloses with traditional group bank up with earth the bottle only the upper end opening change the equal opening of lower extreme into, be favorable to the root the plant of fragility shift. Be provided with the grid under on the bottle lid, the growth of banking up the seedling for the group with earth provides an independent growth space, and the root system solely becomes integrative separately, and the root system is pulled apart together easily to the friendship knot of avoiding plant tissue culture in -process plant roots labyrinth when going out the bottle. And the bottleneck is wide, inoculation operating time is practiced thrift in the operation of being convenient for, is convenient for wash and reuse, practices thrift the manpower.

Description

A kind of plant tissue culture bottle of upper and lower opening

Technical field

This utility model belongs to field of plant tissue culture technique, is specifically related to the plant tissue culture bottle of a kind of upper and lower opening.

Background technology

Plant tissue culture also known as plant tissue culture, i.e. plant Sterile Culture Methods Used.It is, according to plant cell, there is totipotency Theory, utilize tissue, cell or the protoplasts such as in vitro organ such as root, stem, leaf, stem apex, flower, the fruit of plant, in nothing Bacterium and suitable synthetic medium and illumination, temperature etc. artificial under the conditions of, callus, adventitious bud, adventitious root can be induced, The technology of the plant that rear formation is complete.

Tissue culture bottle is a kind of device that plant tissue culture is conventional, is also seed bottle (being commonly called as: bacterium bottle), is used for cultivating seed plumelet A kind of container of breed of variety, container is nontoxic, safety, health, transparency and transmittance relatively good.By 15～25 during use Seedling is inoculated in a tissue culture bottle, and along with seedling is gradually grown up, the warped roots of test tube Seedling is bottom tissue culture bottle, deep-rooted Root intricate combine together, form one at bottle bottom dish, be extremely difficult to during bottle outlet, can to the root of test tube Seedling, stem, Leaf causes the biggest damage, and transplanting survival rate is greatly reduced, simultaneously because inconvenient operation when warped roots causes bottle outlet, Wash away culture medium inconvenience after bottle outlet, cause and transplant time-consuming taking a lot of work.

Utility model content

In order to overcome existing tissue culture bottle only upper end open, bottleneck is little, and in plant tissue culture course, root system of plant is crisscross Complicated combines together, and easily pulls apart the deficiency of root system during bottle outlet, and this utility model provides the plant group of a kind of upper and lower opening Training bottle.This tissue culture bottle simple in construction, changes traditional tissue culture bottle only upper end open into upper and lower side equal opening, and on lower bottle cap It is provided with grid.More fragile plant shifts lower bottle cap beneficially root, and grid provides one solely to the growth of tissue cultured seedling Vertical growing space, respective root system alone becomes one, it is to avoid in plant tissue culture course, the complicated knot of root system of plant exists Together, root system is easily pulled apart during bottle outlet.And bottle transparency and transmittance are all preferable, can significantly improve the efficiency of light energy utilization.

This utility model solves its technical problem and be the technical scheme is that

The plant tissue culture bottle of a kind of upper and lower opening, including transparent bottle, bottle upper end is provided with bottleneck, in bottleneck connection Bottle cap, upper bottle cover is provided with passage；Described bottle bottom arranges under shed, and under shed connects lower bottle cap, and lower bottle cap sets It is equipped with grid；Being square in the middle of grid, the grid external shape adjacent with bottle edge is with the change of bottle edge radian Change；The quantity of grid is determined by the size of tissue culture bottle；Square bottom side length 2.0cm ~ 2.2cm, high 2.8cm ~ 3.2cm.Bottle Surfaces externally and internally is smooth, forms for transparent material, it is simple to the globule drips, and more conducively printing opacity and observation can significantly improve plant luminous energy Utilization rate；Upper bottle cover uses rotary sealing, and sealing is closely, easy to operate, can realize stacking by tissue culture bottle, make full use of training Support case, the space of culturing rack, it is simple to mass propgation；Passage realizes the inside and outside ventilation of tissue culture bottle, in adding bottle and ambient atmos Exchange capacity, reduce the moisture content in bottle, increase tissue cultured seedling photosynthetic capacity；Under shed connects lower bottle cap, when needing Plant within bottle is removed, it is only necessary to being opened by lower bottle cap, the plant that beneficially root is more fragile shifts；Lower bottle cap On be provided with grid, provide an independent growing space to the growth of tissue cultured seedling, respective root system alone become one, it is to avoid plant In tissue culture procedures, root system of plant is complicated combines together, and easily pulls apart root system during bottle outlet.

It is provided with through hole, it is ensured that supplementary nutritional solution can be distributed in inside each grid uniformly on the sidewall of grid.

A diameter of 8 ~ the 15mm of passage, by the bacteriological filtration ventilated membrane capping that aperture is 0.2 ~ 0.3 μm, good permeability, bacteriological filtration is imitated The best, all dust granules including antibacterial can be filtered, reach the purpose purifying and ventilating.

Described bottle, upper bottle cover, lower bottle cap and grid are made up of high-purity polypropylene material, can enter it under High Temperature High Pressure Row sterilization, transparency is higher, not cracky.

The utility model has the advantages that:

(1) this utility model bottleneck is wide, it is simple to operation, saves the inoculation operating time, it is simple to clean and reuse, and saves Manpower；

(2) tissue culture bottle of this utility model design upper and lower opening, changes the structure of tradition tissue culture bottle only upper end open, tears open Load bottle cap more convenient, when needing to be removed by the plant within bottle, it is only necessary to being opened by lower bottle cap, beneficially root is more fragile Plant shift；

(3) this utility model is provided with grid on lower bottle cap, provides an independent growth to the growth of tissue cultured seedling Space, respective root system alone becomes one, it is to avoid in plant tissue culture course, root system of plant is complicated combines together, bottle outlet Shi Rongyi pulls apart root system；

(4) it is provided with through hole on this utility model grid, it is ensured that supplementary nutritional solution can be distributed in each grid uniformly Inside lattice；

(5) this utility model is provided with passage, adds the interior exchange capacity with ambient atmos of bottle, without sealed membrane, Saving operating procedure, it is simple to regulation and control, the later stage can uncap cultivations, breaks through space restriction；

(6) this utility model can be deposited in stacking, makes full use of incubator, culturing rack space, it is simple to mass propgation；

(7) this utility model material and shape design, it is simple to the globule drips, more conducively printing opacity and observation, not cracky, behaviour Make safer, light weight, it is simple to carrying.

Accompanying drawing explanation

Fig. 1 is the diametrically opposite sectional view of this utility model；

Fig. 2 is the top view of this utility model lower bottle cap.

Symbol description in figure: 1, bottle；2, upper bottle cover；3, passage；4, under shed；5, lower bottle cap；6, grid；7, logical Hole.

Detailed description of the invention

Below in conjunction with accompanying drawing, this utility model is further described:

With reference to Fig. 1, the plant tissue culture bottle of a kind of upper and lower opening, including transparent bottle 1, bottle 1 upper end is provided with bottleneck, Bottleneck connects upper bottle cover 2, and upper bottle cover 2 is provided with passage 3, and a diameter of 10mm of passage 3 is saturating by the bacteriological filtration that aperture is 0.25 μm Air film covers；Bottle 1 bottom arranges under shed 4, and under shed 4 connects lower bottle cap 5, and lower bottle cap 5 is provided with grid 6, grid 6 Through hole 7 it is provided with on sidewall.

With reference to Fig. 2, being square in the middle of described grid 6, grid 6 external shape adjacent with bottle 1 edge is with bottle Body 1 edge radian changes and changes, square bottom side length 2.1cm, high 3cm；Described bottle 1, upper bottle cover 2, grid 6 and lower bottle cap 5 are made up of high-purity polypropylene material.

It is last that it is noted that obviously above-described embodiment is only for clearly demonstrating this utility model example, And not restriction to embodiment.For those of ordinary skill in the field, the most also may be used To make other changes in different forms.Here without also cannot all of embodiment be given exhaustive.And thus That is amplified out obviously changes or changes among still in protection domain of the present utility model.

Claims (2)

1. a plant tissue culture bottle for upper and lower opening, including transparent bottle, bottle upper end is provided with bottleneck, and bottleneck connects upper bottle Lid, upper bottle cover is provided with passage；It is characterized in that, described bottle bottom arranges under shed, and under shed connects lower bottle cap, under Grid it is provided with on bottle cap；Being square in the middle of described grid, the grid external shape adjacent with bottle edge is with bottle Edge radian changes and changes；Square bottom side length 2.0cm ~ 2.2cm, high 2.8cm ~ 3.2cm；It is provided with logical on the sidewall of grid Hole；A diameter of 8 ~ the 15mm of described passage, by the bacteriological filtration ventilated membrane capping that aperture is 0.2 ~ 0.3 μm.

The plant tissue culture bottle of a kind of upper and lower opening the most as claimed in claim 1, it is characterised in that described bottle, upper bottle cover, Lower bottle cap and grid are made up of high-purity polypropylene material.