CN205443274U - Box circulation foam photobioreactor - Google Patents
Box circulation foam photobioreactor Download PDFInfo
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- CN205443274U CN205443274U CN201521130195.8U CN201521130195U CN205443274U CN 205443274 U CN205443274 U CN 205443274U CN 201521130195 U CN201521130195 U CN 201521130195U CN 205443274 U CN205443274 U CN 205443274U
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Abstract
The utility model provides a box circulation foam photobioreactor, include: a box is equipped with a feed inlet and a discharge gate, just the feed inlet with the discharge gate is located the relative both sides of box, the polylith guide plate, vertical set up in in the box, will the box falls into a plurality of compartments, be equipped with a plurality of water conservancy diversion holes on the guide plate, a plurality of air distributor, set up in the bottom half, and the interval set up in in the compartment, a foam groove, set up in the box top, and with the box intercommunication, be equipped with the foam discharge port on the foam groove.
Description
Technical field
This utility model relates to a kind of box Circulation foam bioreactor.
Background technology
Bioreactor is can effectively to utilize luminous energy, completes the reactor of the processes such as specific biomass cultivation.Illumination biomass are cultivated and are widely used value in FUTURE ENVIRONMENT and energy field.
One important application of bioreactor is for biological High Density Cultivation such as algae.The artificial culture using bioreactor to carry out marine microalgae is to solve the important channel that restriction marine microalgae large-scale cultivation amplifies; for the acquisition of the important living resources such as food proteins, medicinal and other raw materials, and the acquisition of biodiesel basis raw material is significant.Additionally, utilize microalgae to reduce discharging CO2, it is main and effective carbon sequestration mode on the earth, is paid much attention to by domestic and international researcher.Utilize biological hydrogen production by photosynthetic bacteria, have do not put oxygen, to produce hydrogen purity high, to the response range width of solar spectrum and can be with multiple biological features such as forming good Tiny ecosystem system of setting up, it is considered as one of green hydrogen source, also obtains the extensive concern of domestic and international researcher.Bioreactor technology has critical meaning for industrialization, the high efficiency of said process.
The design of bioreactor is complicated more than traditional chemical reactor, except relating to quality transmission and mixing, the type of flow, kinetics, in addition to the problem such as temperature control, it is necessary to consideration this key factor of light radiation.Luminous energy reduces rapidly along with the increase of the degree of depth of liquid in the liquid phase, and the most only on the top layer of liquid phase, photoreaction could effectively be carried out.Therefore Photoreactor design it is crucial that the most effectively utilize light source, and meet the needs of mass transfer and mixing simultaneously.In recent years, various forms of bioreactor is developed.Dividing from structure, bioreactor has the various structures such as pool, duct type, flat/box, pillar.
Since the nineties in last century, with pipeline reactor and plate-type reactor, the closed Photoreactor as representative develops rapidly, has the most formed some patents and research report.Pipeline reactor typically by clear hard plastics or glass, lucite sealed tube, bends to difformity, utilizes transparent pipeline, carries out light cultivation by natural light or artificial light source.The pipeline reactor being placed on conical support reported such as Japan Patent JP9121835-A (1995) and JP2743316-B2；Bottom horizontal flow sheet pipeline reactor (the Appl.Microbiol.Biotechnol of the report such as O.Pulz, 2001,58:287-293), ring form pipeline type reactor (the Int.Biodeter.Biodegra. of the report such as L.Travieso, 2001,47:151-155), the shell and tube reactor that world patent WO2008010737-A1 (2008) reports.It is big that duct type has illuminating area, advantage, and the research that is widely used in terms of light-catalyzed reaction and biomass cultivation such as can operate continuously.But for ensureing that light source is sufficient, the diameter of sealed tube is generally less than 0.1m, and its cost is high, not easy cleaning simultaneously.
Board-like (box) reactor is made up of the plate case structure of single or multiple combinations, or is divided into multiple chamber by installation deflection plate in casing.R.D.Qrtega in 1986 et al. reported first flat plate photobioreactor (Biomass, 1986, (10): 14-156.), hereafter the board-like Photoreactor of multi-form is in the news in succession, the inner light source boxboard typed reactor of the report such as patent such as United States Patent (USP) US005104803A (1992), US0059932A1 (2003), italian patent IT124175 (1994), the built-in baffled reactor of the different structure of the report such as Chinese invention patent CN1880442A (2006), world patent WO2009069967-A2 (2009)；Additionally pertinent literature reports the plate-type reactor of some new models, the vertical caulking groove plate-type reactor (BioresourceTech. reported such as Tredici etc., 1991,38:153-160), and the board-like bioreactor of inclination bubbling of the report such as Q.Hu (J.Appl.Phycol., 1996,8:139-145), the L-type Flat photobioreactor (BioresourceTech., 2006,97:322-329) etc. of the report such as S.R.Chae.These reactors have that the efficiency of light energy utilization is high, structure is relatively easy, can need, by advantages such as unit modular design structures, to be suitable for High Density Cultivation according to cultivation.But the type reactor exists serious problems on industry is amplified: for the reactor of simple in construction, on direction of illumination, the mixing intensity of reactant/biomass and liquid phase is low, mix mass transfer effect after amplifying box volume and be deteriorated and the reduction of illumination utilization rate；And the design of baffle plate type adds the resistance of fluid, it is easily formed dead band in reactor content, and should not clear up.
Post holding reactor is based on airlift reactor.Owing to inside reactor is along the circulation of guide shell, in reactor, less turbulence is high, is effectively increased mass transfer and mixing efficiency, and oxygen desorbing is good, CO2Utilization rate high, and the structure of reactor of the type is simple, has been widely used in industries such as fermentations, has had industrialized good basis；But its surface area accepting illumination is less, the utilization rate of light need not, if improve light utilization rate, such reactor then has a good application prospect.
In sum, application at present and the bioreactor of exploitation, still suffer from various problem, develop the efficiency of light energy utilization high, mixing simultaneously and mass transfer effect are preferable, and amplification suitable for industrialized and the Photoreactor of operation remain the key technical problem needing to solve.
Utility model content
The purpose of this utility model is: propose a kind of box Circulation foam bioreactor for illumination cultivation microorganism, to improve the utilization rate of luminous energy, ensure that reactor has the hydrodynamic characteristics such as good mixing and transmission, can complete the preliminary concentration of biomass, reactor easily industry is amplified and operation simultaneously.
To achieve these goals, the one box Circulation foam bioreactor that this utility model provides, including:
One casing, is provided with a charging aperture and a discharging opening, and described charging aperture is positioned at the opposite sides of described casing with described discharging opening；
Polylith deflector, is vertically arranged in described casing, and described casing is divided into multiple compartment, and described deflector is provided with multiple pod apertures；
Multiple gas distributors, are arranged at described box bottom, and are arranged at intervals in described compartment；
One foam tank, is arranged at above described casing, and connects with described casing, and described foam tank is provided with foam outlet.
Above-mentioned box Circulation foam bioreactor, the top of described deflector, bottom be equal and leaves gap between described casing.
Above-mentioned box Circulation foam bioreactor, described deflector is divided into low level deflector and high-order deflector, the base of the described high-order deflector distance away from described box bottom to be more than the base of the described low level deflector distance away from described box bottom.
Above-mentioned box Circulation foam bioreactor, the base of described high-order deflector is higher than the distance on the base of low level deflector to box bottom by 20%～100% to the distance of described box bottom.
Above-mentioned box Circulation foam bioreactor, described low level deflector and described high-order deflector are spaced, if the charging aperture of described reactor at left end, discharging opening at right-hand member, the described high-order deflector being positioned at left side forms an ascent stage compartment with the described low level deflector being positioned at right side, and the described low level deflector being positioned at left side forms a descending branch compartment with the described high-order deflector being positioned at right side.
Above-mentioned box Circulation foam bioreactor, described gas distributor is positioned at described ascent stage compartment.
Above-mentioned box Circulation foam bioreactor, described foam tank one end is concordant with described casing, and another section is longer than described casing, and the side of the foam tank growing part is provided with described foam outlet.
Above-mentioned box Circulation foam bioreactor, described discharging opening and described foam outlet homonymy.
Above-mentioned box Circulation foam bioreactor, described casing be longitudinal section be the elongated slot of isosceles trapezoid；The ratio of the trapezoidal upper end and width of going to the bottom is 1～1.4:1, and width range of going to the bottom is 10～25cm；The height of casing and the ratio gone to the bottom are 4～10:1.
Above-mentioned box Circulation foam bioreactor, the base of the described deflector distance away from described box bottom is 0.5～1:1 with the ratio of the width of going to the bottom of described casing longitudinal section, and the distance at casing top described in the top margin of described deflector is 0.5～2:1 with the ratio of the upper bottom width degree of described casing longitudinal section.
Above-mentioned box Circulation foam bioreactor, described deflector is provided with along equally distributed three pod apertures of size such as described deflector longitudinal midlines, described deflector height is 8～11:1 with the ratio in the aperture of described pod apertures, and the lower of described deflector is 1.5～3:1 along wide and aperture ratio.
Above-mentioned box Circulation foam bioreactor, described foam tank be longitudinal section be the elongated slot of isosceles trapezoid；The centrage of described foam tank and described casing longitudinal section overlaps；Go to the bottom in described foam tank longitudinal section and the width ratio of the upper end of described casing longitudinal section is 1～1.5:1, and on described foam tank longitudinal section, the end and width ratio of going to the bottom are 1～2:1；The described foam tank degree of depth is the 15%～25% of box height.
Above-mentioned box Circulation foam bioreactor, described ascent stage compartment is 1～1.3:1 with the length ratio of described descending branch compartment, and the length of described ascent stage compartment and the aspect ratio of described casing are 1:4～10.
Above-mentioned box Circulation foam bioreactor, the material of described casing is glass or lucite, the top seal or unlimited of described casing.
The beneficial effects of the utility model:
1) froth bed can improve the concentration of culture, reduces light loss simultaneously, thus can improve optical energy utilization efficiency and average cell density；
2) by circulation in the exchange of liquid layer and froth bed forming material (including gas or other culture materials dissolved) and transmission, higher mass-transfer efficiency and the cultivation of oxygen desorption rate, beneficially biomass can be obtained；
3) deflector is spaced apart by high and low, the most longitudinal perforate, can realize mixing uniform of liquid；
4) during discharge, directly product or biomass are carried out concentration, it is achieved that the coupling reacted and separate, improve efficiency and the economy of process.
Below in conjunction with the drawings and specific embodiments, this utility model is described in detail, but not as to restriction of the present utility model.
Accompanying drawing explanation
Fig. 1 is the front view of box Circulation foam bioreactor of the present utility model；
Fig. 2 is the side view of box Circulation foam bioreactor of the present utility model.
Wherein, reference
Casing 1
Gas distributor 2
Deflector 3
High-order deflector 31
Low level deflector 32
Foam tank 4
Discharging opening 5
Foam outlet 6
Charging aperture 7
Descending branch compartment 8
Ascent stage compartment 9
Detailed description of the invention
This utility model provide box Circulation foam Photoreactor, including casing 1, gas distributor 2, deflector 3, foam tank 4.
The casing of reactor is long groove shape, and its longitudinal section is isosceles trapezoid, and on it, the end and width ratio of going to the bottom are 1:1～1.4:1, and for ensureing irradiation and the flowing of light, its width range of going to the bottom is 10～25cm；Height (trapezoidal height) and the ratio gone to the bottom of casing are 4:1～10:1；Such design can improve the light distribution at reactor, obtains preferable hydrodynamic characteristic.The length of casing was determined by the amount of cultivation or the time of staying.Be respectively arranged at two ends with charging aperture 7 and discharging opening 5, charging aperture 7 and the discharging opening 5 of casing are arranged at the opposite sides of casing, and the charging aperture 7 in the present embodiment is positioned at lower section on the left of casing, and discharging opening 5 is positioned at top on the right side of casing.
Keep at a certain distance away in casing 1 several deflectors 3 vertically-mounted, deflector 3 and casing 1 front-back intersects vertically, and casing 1 is divided into several compartments；Those deflector 3 dual-sides are fixed on the casing of reactor, and deflector 3 bottom all leaves gap with box bottom, deflector top with casing top.The base of deflector 3 is 0.5:1～1:1 to the go to the bottom ratio of width of the distance of box bottom and casing cross section, and in the distance of the top of deflector 3 to casing top and casing cross section, the ratio of bottom width degree is 0.5:1～2:1；The height of all deflectors 3 is the most identical, but a point high position and two kinds of mounting means of low level are spaced, base, the distance of top to box bottom according to those deflectors 3 are different, deflector 3 is divided into high-order deflector 31 and low level deflector 32, high-order deflector 31 is spaced with low level deflector 32, the base of high-order deflector 31 distance bottom casing 1 is more than the base of low level deflector 32 distance away from box bottom, and the former exceeds 20%～100% to the distance of box bottom than the latter.At the cell bottom center that deflector is separated, interval installation gas distributor 2, if the charging aperture of reactor at left end, discharging opening at right-hand member, then the left side of compartment be a high position deflector 31, right side is low level deflector 32, and the height at its jeting area center is between two deflector lower limbs.The high-order deflector 31 being positioned at left side forms an ascent stage compartment with the low level deflector 32 being positioned at right side, and the low level deflector 32 being positioned at left side forms a descending branch compartment with the high-order deflector 31 being positioned at right side, and gas distributor 2 is arranged in ascent stage compartment 9.By mounting guiding board 3 and gas distributor 2, can be formed in the casing 1 of reactor such as the shown circulation in Fig. 1 and the flowing of overall plug-flow.Its principle is: after the compartment equipped with gas distributor 2 is ventilated, should reduce every the equal phase density of plan of indoor liquid, thus and do not have fluid in the adjacent compartments of air-blowing to form density contrast, principle according to Airlift circulating reactor, density contrast will promote fluid from the ascent stage compartment (ascent stage) of air-blowing to descending branch compartment 8 (descending branch) flowing not having air-blowing, thus forms circulation.Simultaneously because convince discharge orifice by patient analysis in the middle of deflector 3, partially liq will enter adjacent compartments by those pod apertures.Shown in Fig. 1, being low level deflector 32 on the right side of gas distributor, bottom gap is little thus flow resistance is big；High-order deflector 31 in the left side of gas distributor, bottom gap is big thus flow resistance is little；This just makes more fluid flow according to the direction of solid line in Fig. 1, thus forms one on apparent from reactor inlet to the plug-flow of outlet.
The upper end of the casing of reactor is foam tank 4, and foam tank 4 connects (such as Fig. 2) with casing 1, and its longitudinal section is also isosceles trapezoid, and the centrage of its centrage and casing longitudinal section overlaps.The width ratio of the upper end that what foam tank longitudinal section was trapezoidal go to the bottom and casing longitudinal section is trapezoidal is 1:1～2:1, and the upper end and width ratio of going to the bottom that foam tank longitudinal section is trapezoidal are 1:1～1.5:1；The degree of depth of foam tank is the 15%～25% of box height；In length, foam tank one end is concordant with casing, and another section grows 10-60cm than casing, and this grows part groove side and installs foam outlet 6, and the biomass in the foam that will collect and foam are discharged, and foam outlet 6 and discharging opening 5 are positioned at homonymy.
Below in conjunction with biomass illumination cultivation, illustrate the mode of operation of this reactor:
In reactor, add culture fluid, make liquid level reach above deflector.Inoculation biomass, add proper amount of surfactant as foam stabiliser, blowing air (or rich in CO2Gas, such as flue gas) cultivate.Under the effect of Airlift circulating, bubble the most constantly disperses, and surfactant is gathered in bubble interface simultaneously, forms froth bed, under the operating condition optimized, froth bed can be made to be located exactly in foam tank on liquid level.Light is injected from the top of reactor and surrounding.Under the common effect of circulation effect and steel foams, in liquid phase, biomass are constantly enriched with in foam liquid phase, the most a part of biomass constantly occur with rupture return liquid layer, this exchange and the mixing of circulation and foam so that each biological cell can well accept illumination；CO in bubble2Constantly it is delivered to rapidly in foam liquid phase, is carried out photosynthetic absorption by biology in foam liquid phase；Accompany circulation and bubble of foam solution updates and constantly swaps with liquid phase main body；With the renewal of foam solution, dissolve nutrient substance in the liquid phase and constantly taken to froth zone, and the metabolite in foam, including the oxygen dissolved, then constantly taken back liquid phase main body, be diluted or desorbing, formed continuous print incubation.Along with fluid is to the plug-flow of outlet side, biomass constantly grow and flow to outlet side, and the biomass reaching incubation time flow out from foam tank outlet 6, after standing froth breaking, and biomass collection, and foam solution can return charging aperture 7 with the merging of fresh feed liquid and recycle.
For further illustrating the effect of the reactor utilizing this utility model to provide, especially exemplified by embodiment once:
Embodiment 1
The present embodiment is foam phase and the light decay situation of liquid phase of this utility model Photoreactor.Reactor is lucite manufacture, and top is opened wide, and main structure parameters is as follows: the 16cm of the upper end that casing longitudinal section is trapezoidal, and go to the bottom 12cm, box height 105cm, casing length 244cm, dischargeable capacity 350L；Casing is divided into 12 compartments by 11 pieces of deflectors, and the length of ascent stage compartment and descending branch compartment 8 is 20cm (distance between the most adjacent two deflectors is 20cm), and Reactor inlet side is ascent stage compartment.The height of deflector is 77cm, and high-order deflector 31 is 12cm away from box bottom, and low level deflector 32 is 8cm away from box bottom, and on deflector, open pore size is 7cm, every two pitchs of holes 14cm, most descends 14cm below pitch-row deflector.Installing membrane type gas distributor at ascent stage compartment, the centre-height of gas distributor areal area is concordant with deflector lower edge, a diameter of 18mm of distributor, and total height is 48mm, and average pore size is 5mm.On foam tank, the end and width of going to the bottom are 20cm, and the degree of depth is 20cm, a length of 260cm.Measurement system is water-air system, wherein contains 0.8 × 108The chlorella (MarineChlorella) of cell/mL.Employing Tween20 is foam stabiliser.Liquid amount is 340L, and ventilation is 30L/min, and when stablizing, liquid phase height is 103cm, and foam height mutually is 5cm；With daylight lamp as light source, incident illumination illumination I0=4200lx.In foam photoreaction of the present utility model, the degree of optical attenuation is less than pure liquid phase.
Embodiment 2
The present embodiment carries out the cultivation of sea growing chlorella (MarineChlorella) for using bioreactor of the present utility model.Reactor with embodiment 1, light from reactor head and surrounding inject, incoming illumination I0=4200lx.Inoculum density is 4.2 × 106Cell/ml, culture medium uses f/2 to improve sea water medium, and adds surfactant Tween20, and concentration is 30mg/L.Initial pH8.2, temperature is room temperature, culture fluid 340L, and ventilation is 30L/min, and when stablizing, height of liquid layer is 103cm, and foam layer height is 3cm.Cultivating 180h under the conditions of this, the growth pattern of algae cell density is as shown in table 1.Situation when wherein matched group is that all liquid phases (liquid level is 106cm) are cultivated in same reactor.After stable, chlorella concentration ratio in the foam is 1.4～1.6.
The cell density growth pattern (10 of table 1 chlorella6cell/mL)
Certainly; this utility model also can have other various embodiments; in the case of without departing substantially from this utility model spirit and essence thereof; those of ordinary skill in the art are when making various corresponding change and deformation according to this utility model, but these change accordingly and deformation all should belong to the scope of the claims appended by this utility model.
Claims (14)
1. a box Circulation foam bioreactor, it is characterised in that including:
One casing, is provided with a charging aperture and a discharging opening, and described charging aperture is positioned at the opposite sides of described casing with described discharging opening；
Polylith deflector, is vertically arranged in described casing, and described casing is divided into multiple compartment, and described deflector is provided with multiple pod apertures；
Multiple gas distributors, are arranged at described box bottom, and are arranged at intervals in described compartment；
One foam tank, is arranged at above described casing, and connects with described casing, and described foam tank is provided with foam outlet.
Box Circulation foam bioreactor the most as claimed in claim 1, it is characterised in that the top of described deflector, bottom be equal and leaves gap between described casing.
Box Circulation foam bioreactor the most as claimed in claim 2, it is characterized in that, described deflector is divided into low level deflector and high-order deflector, the base of the described high-order deflector distance away from described box bottom to be more than the base of the described low level deflector distance away from described box bottom.
Box Circulation foam bioreactor the most as claimed in claim 3, it is characterised in that the distance on the base of described high-order deflector to described box bottom than the base of described low level deflector to the distance high 20%～100% of box bottom.
Box Circulation foam bioreactor the most as claimed in claim 4, it is characterized in that, described low level deflector and described high-order deflector are spaced, if the charging aperture of described reactor at left end, discharging opening at right-hand member, the described high-order deflector being positioned at left side forms an ascent stage compartment with the described low level deflector being positioned at right side, and the described low level deflector being positioned at left side forms a descending branch compartment with the described high-order deflector being positioned at right side.
Box Circulation foam bioreactor the most as claimed in claim 5, it is characterised in that described gas distributor is positioned at described ascent stage compartment.
Box Circulation foam bioreactor the most as claimed in claim 1, it is characterised in that described foam tank one end is concordant with described casing, and another section is longer than described casing, the side of the foam tank growing part is provided with described foam outlet.
Box Circulation foam bioreactor the most as claimed in claim 7, it is characterised in that described discharging opening and described foam outlet homonymy.
Box Circulation foam bioreactor the most as claimed in claim 1, it is characterised in that described casing be longitudinal section be the elongated slot of isosceles trapezoid；The ratio of the trapezoidal upper end and width of going to the bottom is 1～1.4:1, and width range of going to the bottom is 10～25cm；The height of casing and the ratio gone to the bottom are 4～10:1.
Box Circulation foam bioreactor the most as claimed in claim 9, it is characterized in that, the base of the described deflector distance away from described box bottom is 0.5～1:1 with the ratio of the width of going to the bottom of described casing longitudinal section, and the distance at the top margin casing top of described deflector is 0.5～2:1 with the ratio of the upper bottom width degree of described casing longitudinal section.
11. box Circulation foam bioreactors as claimed in claim 1, it is characterized in that, described deflector is provided with along equally distributed three pod apertures of size such as described deflector longitudinal midlines, described deflector height is 8～11:1 with the ratio in the aperture of described pod apertures, and the lower of described deflector is 1.5～3:1 along wide and aperture ratio.
12. box Circulation foam bioreactors as claimed in claim 10, it is characterised in that described foam tank be longitudinal section be the elongated slot of isosceles trapezoid；The centrage of described foam tank and described casing longitudinal section overlaps；Go to the bottom in described foam tank longitudinal section and the width ratio of the upper end of described casing longitudinal section is 1～1.5:1, and on described foam tank longitudinal section, the end and width ratio of going to the bottom are 1～2:1；The described foam tank degree of depth is the 15%～25% of box height.
13. box Circulation foam bioreactors as claimed in claim 5, it is characterised in that described ascent stage compartment is 1～1.3:1 with the length ratio of described descending branch compartment, the length of described ascent stage compartment and the aspect ratio of described casing are 1:4～10.
14. box Circulation foam bioreactors as claimed in claim 1, it is characterised in that the material of described casing is glass or lucite, the top seal or unlimited of described casing.
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| CN201521130195.8U CN205443274U (en) | 2015-12-30 | 2015-12-30 | Box circulation foam photobioreactor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2022095267A1 (en) * | 2020-11-09 | 2022-05-12 | 河南农业大学 | Novel pump-free internal circulation biological hydrogen photoproduction reactor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2022095267A1 (en) * | 2020-11-09 | 2022-05-12 | 河南农业大学 | Novel pump-free internal circulation biological hydrogen photoproduction reactor |
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