CN205420373U - Cell recovery device - Google Patents
Cell recovery device Download PDFInfo
- Publication number
- CN205420373U CN205420373U CN201620228449.8U CN201620228449U CN205420373U CN 205420373 U CN205420373 U CN 205420373U CN 201620228449 U CN201620228449 U CN 201620228449U CN 205420373 U CN205420373 U CN 205420373U
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- China
- Prior art keywords
- spacing hole
- hold
- cell recovery
- recovery device
- down bars
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Abstract
The utility model relates to a cell recovery device, including a pair of mount, opened spacing hole A and spacing hole B respectively on two mounts, spacing hole A and spacing hole B symmetry set up and the fixed flat fixed axle that sets up in same genshui of inserting of while, fix the hold -down bars that is equipped with a perpendicular to fixed axle on lieing in two fixed axle parts between the mount, and the activity is inserted and is equipped with a holder on the hold -down bars, the holder includes a montant and sets up deposits a plurality of clips of managing freezing for the centre gripping of montant lower extreme, and the top part of montant is the handle, and it has the mobile through -hole of establishing on the hold -down bars of inserting to be used for realizing that whole holder can sway for the hold -down bars to open in the montant part below the handle. Compared with the prior art, the utility model discloses a cell recovery device has not only improved the efficiency that the cell is come back to life or consciousness, reduces the probability that the cell pollutes, and whole process is very safe high -efficient, and has made things convenient for operator's observation.
Description
Technical field
This utility model relates to technical field of cell biology, particularly to a kind of cell recovery device.
Background technology
Cell recovery is to take out the frozen cryopreservation tube having cell and immerse to make its fast melt in about 38 DEG C warm water from liquid nitrogen, recovers the process of cell viability, and general step is (1) operator's wearing spectacles and glove, takes out cryovial from liquid nitrogen container；(2) put into rapidly in about 38 DEG C warm water, and frequently shake, made it melt completely in 1 minute, take out cell the most under aseptic conditions.
But in current actual mechanical process, owing to lacking unified operating provision, there is no a kind of general, unified cell recovery equipment yet, the concrete operational motion in different experiments room is not quite similar, such as (1) some operator is with Frostbite preventing gloves, direct hands takes out cryopreservation tube from liquid nitrogen and operates, the shortcoming of this operational motion is: one to two solencytes of once can only recovering, the visual field is bad simultaneously, easily below cryopreservation tube immersed in liquid level, cause cell contamination, be also inconvenient to observe cryopreservation tube inner cell liquid status；(2) some operator is free-hand operates by cryopreservation tube, and the shortcoming of this operational motion is: be easy to cold injury hands, and as easy as rolling off a log be subjected to the cryopreservation tube that liquid nitrogen gasification effect causes and explode the injury caused；(3) also having operator directly to throw away in water-bath after directly cryopreservation tube being taken out from liquid nitrogen, the shortcoming of this operational motion is: easily causes cell contamination, and can not swing in time and be unfavorable for thawing rapidly, etc..
In order to overcome above-mentioned technical problem, this utility model provides a kind of can make cell recovery processing efficient, the convenient cell recovery device observing, stablizing safety.
Utility model content
The purpose of this utility model is not enough in order to overcome present in above-mentioned existing cell recovery process, it is provided that a kind of can make cell recovery processing efficient, convenient observe, the cell recovery device of stable safety.
To achieve these goals, this utility model have employed techniques below scheme:
A kind of cell recovery device, including a pair fixed mount, two fixed mounts have spacing hole A and spacing hole B, spacing hole A and spacing hole B is symmetrical arranged and fixes simultaneously and is inserted with same horizontally disposed fixing axle, being fixed with a hold-down bars being perpendicular to fixing axle on the fixing shaft portion between two fixed mounts, on hold-down bars, activity is inserted with a clamper；Described clamper includes a montant and is arranged on the multiple clips in order to clamp cryopreservation tube of montant lower end, the upper section of montant is handle, the montant part below handle has the movable through hole being plugged on hold-down bars and can wave relative to hold-down bars in order to realize whole clamper.
In experimentation after cell recovery and recovery, thermostat water bath will be used, owing to the water in thermostat water bath is that operator adds, therefore the liquid level of the water in the thermostat water bath of different experiments room is also different, and during cell recovery, require that cell can quick-thawing, it is accomplished by the Cell sap build-up ice in cryopreservation tube to submerge under the warm water liquid level of about 38 DEG C as far as possible, but again due to liquid nitrogen gasification, tiny gap usually can be produced between lid and the tube wall of cryopreservation tube, if cryopreservation tube being completely immersed in and being likely to result in cell contamination, therefore, in experimentation, cryopreservation tube can not all submerge under liquid level again, visible the highest to cryopreservation tube status requirement in water in operation；nullIn this device，The height of fixing axle is regulated by the spacing hole A on fixed mount and spacing hole B，The height adjusting fixing axle i.e. have adjusted the height of clamper，Namely have adjusted cryopreservation tube position in water，When specifically used，Can first be clipped on the clip of clamper with an empty cryopreservation tube before this cell recovery，Then the height of the fixing axle of regulation，Cryopreservation tube is made to be in suitable liquid level in water-bath，Then change and place the cryopreservation tube containing freeze-stored cell，The most once can clamp multiple，Then by the artificial handle portion swung on clamper，The cryopreservation tube driving clamper sandwiched swings in warm water，So that cells frozen storing liquid thaws rapidly，As can be seen here，Cell recovery device of the present utility model，Not only increase the efficiency of cell recovery，Reduce the probability of cell contamination，Whole process is fool proof efficiently，And facilitate operator's observation.
Montant described here can be one-body molded by mould with the handle on montant, it is also possible to separately manufactured, in order to improve operator's comfort level when hand-held handle, handle portion outer cover is provided with plastic sheath；Described clip preferably uses the metal material that heat conduction is good, and such as copper, ferrum etc., and the clamping dynamics of this clip is relatively big, can clamp the cryopreservation tube of different size and can prevent cryopreservation tube from slipping, it is ensured that the stability of cryopreservation tube during cell recovery.
It is preferred that the two ends part of described fixing axle is helicitic texture, the most at least part of helicitic texture of helicitic texture at two ends is positioned at the outside of homonymy fixed mount, is provided with nut being positioned in the helicitic texture part outside homonymy fixed mount cooperation.
By the structure of screw thread and nut, fixing axle is firmly secured on two fixed mounts, the not only height of the fixing axle of convenient regulation, meanwhile, be also convenient for the distance between two fixed mounts of size adjustment according to thermostat water bath, nut here preferably be wing nut.
It is preferred that described spacing hole A and spacing hole B is a vertical elongate holes.
The height that fixing axle can be adjusted to has more selection, it is adaptable to more concrete experimental conditions.
It is preferred that described spacing hole A and spacing hole B is by the multiple holes composition being vertically arranged.
The height of fixing axle can be adjusted more accurately, and sufficiently stable.
It is preferred that be respectively marked with unified scale on spacing hole A and spacing hole B.
The same height is transferred at the two ends conveniently and accurately making fixing axle, it is ensured that fixing axle is horizontal.
It is preferred that described hold-down bars is horizontally disposed with, plane that hold-down bars and fixing axle are formed and plane-parallel.
This set facilitates the swing of clamper.
It is preferred that described hold-down bars is positioned at the point midway of fixing axle.
The realization that clamper can be more stable is so made to swing.
It is preferred that be respectively equipped with a limit stick on the fixing axle be positioned at hold-down bars both sides, when clamper inserts hold-down bars, there is gap between clamper and limit stick to prevent clamper from too swinging.
On the premise of making cell recovery quick-thawing, turn avoid amplitude of fluctuation excessive, reduce the probability of cell contamination.
It is preferred that the plurality of clip is arranged with montant for the center of circle to external radiation.
More clip so can be set, clamp more cryopreservation tube, improve the efficiency of cell recovery.
It is preferred that described clip at least includes 3-10.
In actual mechanical process, seldom can carry out the cell thawing process of majority when of recovery cell every time, the clip used at least includes 3, i.e. each three pipe cryopreservation tubes of at least can recovering, need after defrosting cell again to carry out following inoculating cell operation as early as possible, so 3 to 10 clips of design enough routine experimentation rooms use.
This utility model have employed having the beneficial effects that of technique scheme:
This utility model provides a kind of can make cell recovery processing efficient, the convenient cell recovery device observing, stablizing safety.nullIn this device，The height of fixing axle is regulated by the spacing hole A on fixed mount and spacing hole B，The height adjusting fixing axle i.e. have adjusted the height of clamper，Namely have adjusted cryopreservation tube position in water，When specifically used，Can first be clipped on the clip of clamper with an empty cryopreservation tube before this cell recovery，Then the height of the fixing axle of regulation，Cryopreservation tube is made to be in suitable liquid level in water-bath，Then change and place the cryopreservation tube containing freeze-stored cell，The most once can clamp multiple，Then by the artificial handle portion swung on clamper，The cryopreservation tube driving clamper sandwiched swings in warm water，So that cells frozen storing liquid thaws rapidly，As can be seen here，Cell recovery device of the present utility model，Not only increase the efficiency of cell recovery，Reduce the probability of cell contamination，Whole process is fool proof efficiently，And facilitate operator's observation.
Accompanying drawing explanation
Fig. 1 is the structural representation that this utility model is overall；
Fig. 2 is structural representation during the non-installing holder of this utility model；
Fig. 3 is the structural representation of clamper；
Fig. 4 is that this utility model is with the use of structural representation during cryopreservation tube.
Detailed description of the invention
With detailed description of the invention, this utility model is described in further detail below in conjunction with the accompanying drawings:
Embodiment:
A kind of cell recovery device, as Figure 1-4, including a pair fixed mount 1, two fixed mounts 1 have spacing hole A11 and spacing hole B12, spacing hole A11 and spacing hole B12 is a vertical elongate holes, and it is respectively marked with unified scale on spacing hole A11 and spacing hole B12, spacing hole A11 and spacing hole B12 is symmetrical arranged and fixes simultaneously and is inserted with same horizontally disposed fixing axle 2, the point midway of fixing axle 2 part between two fixed mounts 1 is fixed with a hold-down bars 3 being horizontally disposed with and being perpendicular to fixing axle 2, plane that hold-down bars 3 and fixing axle 2 are formed and plane-parallel, on hold-down bars 3, activity is inserted with a clamper 4；Described clamper 4 includes a montant 41 and is arranged on 4 clips 42 arranged to external radiation with montant 4 for the center of circle in order to clamp cryopreservation tube 5 of montant 41 lower end, the upper section of montant 41 is handle 411, montant 41 part below handle 411 has the movable through hole 412 being plugged on hold-down bars 3 and can wave relative to hold-down bars 3 in order to realize whole clamper 4.
In order to prevent clamper from too swinging in water, cause cell contamination, the fixing axle 2 being positioned at hold-down bars 3 both sides is respectively equipped with a limit stick 6, when the montant 41 of clamper 4 inserts hold-down bars 3, leaving gap between montant 41 and limit stick 6, interval, gap is 3mm-10mm；Above-mentioned fixing axle 2, hold-down bars 3 and limit stick 6 can be one-body molded.
The two ends part of fixing axle 2 is helicitic texture 7, and the most at least part of helicitic texture of helicitic texture 7 at two ends is positioned at the outside of homonymy fixed mount 1, coordinates and be provided with wing nut 8 in helicitic texture 7 part being positioned at outside homonymy fixed mount 1.
In this device, the height of fixing axle 2 is regulated by the spacing hole A11 on fixed mount 1 and spacing hole B12, the height adjusting fixing axle 2 i.e. have adjusted the height of clamper 4, namely have adjusted the cryopreservation tube 5 position in water, when specifically used, can first be clipped on the clip 42 of clamper 4 with an empty cryopreservation tube before this cell recovery, then the height of the fixing axle 2 of regulation, cryopreservation tube is made to be in suitable liquid level in water-bath, then change and place the cryopreservation tube 5 containing freeze-stored cell, the most once can clamp multiple, then by artificial handle 411 part swung on clamper 4, the cryopreservation tube 5 driving clamper 4 sandwiched swings in warm water, so that cells frozen storing liquid thaws rapidly；As can be seen here, cell recovery device of the present utility model, not only increase the efficiency of cell recovery, reduce the probability of cell contamination, whole process is fool proof efficiently, and facilitates operator's observation.
Claims (10)
1. a cell recovery device, it is characterized in that: include a pair fixed mount, two fixed mounts have spacing hole A and spacing hole B, spacing hole A and spacing hole B is symmetrical arranged and fixes simultaneously and is inserted with same horizontally disposed fixing axle, being fixed with a hold-down bars being perpendicular to fixing axle on the fixing shaft portion between two fixed mounts, on hold-down bars, activity is inserted with a clamper；Described clamper includes a montant and is arranged on the multiple clips in order to clamp cryopreservation tube of montant lower end, the upper section of montant is handle, the montant part below handle has the movable through hole being plugged on hold-down bars and can wave relative to hold-down bars in order to realize whole clamper.
A kind of cell recovery device the most according to claim 1, it is characterized in that: the two ends part of described fixing axle is helicitic texture, the most at least part of helicitic texture of helicitic texture at two ends is positioned at the outside of homonymy fixed mount, is provided with nut being positioned in the helicitic texture part outside homonymy fixed mount cooperation.
A kind of cell recovery device the most according to claim 1, it is characterised in that: described spacing hole A and spacing hole B is a vertical elongate holes.
A kind of cell recovery device the most according to claim 1, it is characterised in that: described spacing hole A and spacing hole B is by the multiple holes composition being vertically arranged.
5. according to a kind of cell recovery device described in any one of claim 3 or 4, it is characterised in that: on spacing hole A and spacing hole B, it is respectively marked with unified scale.
A kind of cell recovery device the most according to claim 1, it is characterised in that: described hold-down bars is horizontally disposed with, plane that hold-down bars and fixing axle are formed and plane-parallel.
A kind of cell recovery device the most according to claim 1, it is characterised in that: described hold-down bars is positioned at the point midway of fixing axle.
A kind of cell recovery device the most according to claim 1, it is characterized in that: on the fixing axle be positioned at hold-down bars both sides, be respectively equipped with a limit stick, when the montant of clamper inserts hold-down bars, leave gap between montant and limit stick to prevent clamper from too swinging.
A kind of cell recovery device the most according to claim 1, it is characterised in that: the plurality of clip is arranged with montant for the center of circle to external radiation.
A kind of cell recovery device the most according to claim 1, it is characterised in that: described clip at least includes 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620228449.8U CN205420373U (en) | 2016-03-23 | 2016-03-23 | Cell recovery device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620228449.8U CN205420373U (en) | 2016-03-23 | 2016-03-23 | Cell recovery device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205420373U true CN205420373U (en) | 2016-08-03 |
Family
ID=56549066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620228449.8U Expired - Fee Related CN205420373U (en) | 2016-03-23 | 2016-03-23 | Cell recovery device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205420373U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635743A (en) * | 2016-12-28 | 2017-05-10 | 浙江省海洋水产研究所 | Bacterium quick-freezing, storing and taking device |
| CN107746799A (en) * | 2017-11-21 | 2018-03-02 | 赛慕特生物工程（上海）有限公司 | A kind of hand-held dry type cell recovery instrument |
| CN109401916A (en) * | 2018-11-22 | 2019-03-01 | 修宝利 | A kind of parallel-moving type cell recovery device |
-
2016
- 2016-03-23 CN CN201620228449.8U patent/CN205420373U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635743A (en) * | 2016-12-28 | 2017-05-10 | 浙江省海洋水产研究所 | Bacterium quick-freezing, storing and taking device |
| CN106635743B (en) * | 2016-12-28 | 2019-04-23 | 浙江省海洋水产研究所 | Bacterium is rapidly frozen access device |
| CN107746799A (en) * | 2017-11-21 | 2018-03-02 | 赛慕特生物工程（上海）有限公司 | A kind of hand-held dry type cell recovery instrument |
| CN107746799B (en) * | 2017-11-21 | 2023-10-13 | 新钥(南京)生物科技有限公司 | Handheld dry cell resuscitator |
| CN109401916A (en) * | 2018-11-22 | 2019-03-01 | 修宝利 | A kind of parallel-moving type cell recovery device |
| CN109401916B (en) * | 2018-11-22 | 2021-04-30 | 东莞十度生物科技有限公司 | Translation formula cell recovery device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160803 Termination date: 20170323 |