CN205074066U - Liquid nitrogen system of grinding - Google Patents
Liquid nitrogen system of grinding Download PDFInfo
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- CN205074066U CN205074066U CN201520785874.2U CN201520785874U CN205074066U CN 205074066 U CN205074066 U CN 205074066U CN 201520785874 U CN201520785874 U CN 201520785874U CN 205074066 U CN205074066 U CN 205074066U
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- grinding
- pestle
- mortar
- liquid nitrogen
- cryostat
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Abstract
The utility model belongs to the technical field of a liquid nitrogen grinder technique of use among physiology, biochemistry, the molecular biology and specifically relates to a liquid nitrogen system of grinding is related to. Its characteristics are including grinding the pestle, grinding pestle periphery be provided with and grind pestle dull polish plastics overcoat, the mortar with grind the pestle and correspond the setting and in the cryostat, be provided with mortar dull polish plastics overcoat on the mortar, the cryostat in still correspond and be provided with the centrifuging tube mounted frame and grind the pestle mounted frame. It can make things convenient for broken various specimen sample effectively fast, especially to the scientific research of big batch sample number, not only can save a large amount of manpowers and time, still has portably, swift, high -efficient and low -cost advantage, and can not lead to the fact the pollution between the specimen sample. This system can use under normal atmospheric temperature, low temperature and super low temperature condition for to the solution of above -mentioned problem more high -efficient, quick, the economy and convenient that becomes.
Description
Technical field
The utility model relates to the liquid nitrogen grinding device technique field used in a kind of physiology, biochemistry, molecular biology, especially relates to a kind of liquid nitrogen grinding system.
Background technology
In the various physiology of modern life science, Food Science and environmental science, biochemistry or molecular biological research, often need fragmentation pre-treatment test specimen being carried out to physics, as the extraction of RNA/DNA, the separation of protein, mineral nutrient element (Na
+, K
+, PO
3 2-, Ca
2+deng) containing the analysis etc. of the mensuration of quantitative analysis, soluble sugar content, the analysis of soluble amino acid, the mensuration of chlorophyll content and various metabolic enzyme activity (as catalase, superoxide dismutase, glutathione reductase etc.), these test specimens comprise plant, animal or soil etc.And the quality of sample broke process directly can affect the accuracy of follow-up test result, as in aging research to the detection of Unit Weight tissue sample RNA or DNA content, if tissue grinder is insufficient, then detected value will be less than normal.Equally, the measurement of other physiology, biochemistry, molecular indexes also can because historrhexis is insufficient and then affect the accuracy of result of the test.In addition, some test also requires to grind under ice bath or ultralow temperature situation, as the mensuration of enzymatic activity and the extraction of RNA.Current, for the solution of various test requirements document to sample broke be: 1. utilize pottery or glass mortar tissue abrasion or grinding limit, limit under condition of ice bath to add liquid nitrogen to maintain ultralow temperature, then ground tissue solution or powder transfer are carried out follow-up test step to centrifuge tube or test tube; 2. special plant equipment such as tissue grinder instrument is utilized to grind, point normal temperature type and ultralow temperature type.Its shortcoming is: 1. for traditional mortar, and when test specimen process number is larger, grinding and transfer all need consuming time, and inefficiency; And all need carefully to clean mortar before and after test, dry, sterilizing, even high-temperature baking (toasting more than 6h as needed 180 DEG C when RNA extracts), therefore expense people requires great effort time-consuming.In addition, as extracted RNA or DNA, when liquid nitrogen is added in ultralow temperature grinding repeatedly, due to strong liquid-nitrogen boiling, ground powder of organizing can be caused everywhere to splash, very easily because operating experience deficiency can cause the cross pollution between sample and sample, and then cause test failure.2. for the plant equipment of special grinding, it involves great expense, and the laboratory of not all or scientific research personnel all can have enough funds to buy, and for the few sample of number, special-purpose machinery grinding needs to expend more liquid nitrogen, does not calculate economically.In the scientific research field of modern forward position, each scientific research project comprises numerous experimental procedures, and completing of each experimental procedure needs at substantial time and painstaking effort; Therefore, cause whole scientific research project period planning to baffle because some annelet goes wrong or make whole previous experiments all that has been achieved is spoiled to be unacceptable.
Summary of the invention
The purpose of this utility model is to provide a kind of liquid nitrogen grinding system for the deficiencies in the prior art.Thus effectively solve the problems of the prior art.
For achieving the above object, the technical scheme that the utility model is taked is: described a kind of liquid nitrogen grinding system, be characterized in comprising grinding pestle, described grinding pestle periphery is provided with grinding pestle frosted plastic overcoat, pestle is corresponding is arranged in cryostat, for mortar and grinding, mortar is provided with mortar frosted plastic overcoat, in described cryostat, also correspondence is provided with centrifuge tube hanger bracket and grinding pestle hanger bracket.
It is expand the grinding pestle head of formula structure that described grinding pestle comprises the handle of upper end and lower end, and handle is connected by screw thread with grinding pestle head, and grinding pestle head upper end is provided with the scalariform annulus for fixing grinding pestle frosted plastic overcoat.
The described grinding pestle head expanding formula structure is for supporting grinding interface grinding pestle frosted plastic overcoat, and described screw thread replaces with hexagonal jack.
Described grinding pestle frosted plastic overcoat is frosted process interface with grinding pestle head contact surface, the neck of grinding pestle frosted plastic overcoat is the imperfect ring structures with scalariform annulus corresponding matching, and grinding pestle frosted plastic overcoat is cast for highstrenghtpiston or resin and formed.
The upper surface of described mortar is corresponding with grinding pestle head is provided with hole of caving in, grind mouth and projection, and mortar bottom is provided with grinding support, grinds support and is connected with cryostat, by fixing rivet; Described mortar frosted plastic overcoat lower surface is corresponding with mortar upper surface is provided with boss, grind muzzle and groove, described mortar frosted plastic overcoat and mortar contact surface are frosted process interface, and mortar frosted plastic overcoat is cast for highstrenghtpiston or resin and formed.
Described cryostat, is made up of inner wall layer, heat-insulation layer and outer wall layer.
Described centrifuge tube hanger bracket is fixedly linked by the first screw and cryostat, and on centrifuge tube hanger bracket, interface is higher than the end face of mortar, centrifuge tube hanger bracket is provided with grinding pestle suppending hole.
Described grinding pestle hanger bracket is fixedly linked by the second screw and cryostat, and on grinding pestle hanger bracket, interface is lower than the top of cryostat, and grinding pestle hanger bracket is provided with muller suppending hole.
The beneficial effects of the utility model are: described a kind of liquid nitrogen grinding system, it can quick and convenient broken various test specimen effectively, especially for the research work of batch samples number, not only can save a large amount of manpowers and time, also there is advantage that is easy, quick, efficient and low cost, and the pollution between test specimen can not be caused.This system can use under normal temperature, low temperature and condition of ultralow temperature, makes to become more efficient, quick, economical and convenient to the solution of the problems referred to above.
Accompanying drawing illustrates:
Fig. 1 is overall structure principle schematic of the present utility model;
Fig. 2 is grinding pestle structural principle schematic diagram of the present utility model;
Fig. 3 is grinding pestle frosted plastic jacket structure principle schematic of the present utility model;
Fig. 4 is grinding example structure principle schematic of the present utility model;
Fig. 5 is the plan structure principle schematic of Fig. 3 of the present utility model;
Fig. 6 is mortar frosted plastic jacket structure principle schematic of the present utility model;
Fig. 7 is cryostat, example structure principle schematic of the present utility model;
Fig. 8 is the front view of Fig. 7 cryostat, example structure of the present utility model;
Fig. 9 is the reverse view of Fig. 7 cryostat, example structure of the present utility model.
Shown in figure: 1. grind pestle; 1-1. handle; 1-1a. screw thread; 1-2. scalariform annulus; 1-3. grinds pestle head; 2. grind pestle frosted plastic overcoat; 3. mortar; 3-1. caves in hole; 3-2. grinds mouth; 3-3. protruding; 3-4. fixing rivet; 3-5. grinds support; 4. mortar frosted plastic overcoat; 4-1. boss; 4-2. grinds muzzle; 4-3. groove; 5. cryostat; 5-1. inner wall layer; 5-2. heat-insulation layer; 5-3. outer wall layer; 6. centrifuge tube hanger bracket; 6-1. grinds pestle suppending hole; Interface on 6-2. centrifuge tube hanger bracket; 6-3. first screw; 7. grind pestle hanger bracket; 7-1. second screw; 7-2. grinds interface on pestle hanger bracket; 7-3. muller suppending hole.
Detailed description of the invention
Below in conjunction with accompanying drawing shownschematically preferred example be described in further detail:
As shown in Fig. 1 to 8, described a kind of liquid nitrogen grinding system, be characterized in comprising grinding pestle 1, described grinding pestle 1 periphery is provided with grinding pestle frosted plastic overcoat 2, pestle 1 is corresponding is arranged in cryostat, 5 for mortar 3 and grinding, mortar 3 is provided with mortar frosted plastic overcoat 4, in described cryostat, 5, also correspondence is provided with centrifuge tube hanger bracket 6 and grinding pestle hanger bracket 7
Further, it is expand the grinding pestle head 1-3 of formula structure that described grinding pestle 1 comprises the handle 1-1 of upper end and lower end, handle 1-1 is connected by screw thread 1-1a with grinding pestle head 1-3, and grinding pestle head 1-3 upper end is provided with the scalariform annulus 1-2 for fixing grinding pestle frosted plastic overcoat 2.
Further, the described grinding pestle head 1-3 expanding formula structure is for supporting grinding interface grinding pestle frosted plastic overcoat 2, and described screw thread 1-1a replaces with hexagonal jack.
Further, described grinding pestle frosted plastic overcoat 2 is frosted process interface with grinding pestle head 1-3 contact surface, the neck of grinding pestle frosted plastic overcoat 2 is the imperfect ring structures with scalariform annulus 1-2 corresponding matching, and grinding pestle frosted plastic overcoat 2 is cast for highstrenghtpiston or resin and formed.
Further, the upper surface of described mortar 3 is corresponding with grinding pestle head 1-3 is provided with the hole 3-1 that caves in, grind mouth 3-2 and protruding 3-3, and mortar 3 bottom is provided with grinding support 3-5, and grinding support 3-5 passes through fixing rivet 3-4 and is connected with cryostat, 5; Described mortar frosted plastic overcoat 4 lower surface is corresponding with mortar 3 upper surface is provided with boss 4-1, grind muzzle 4-2 and groove 4-3, described mortar frosted plastic overcoat 4 is frosted process interface with mortar 3 contact surface, and mortar frosted plastic overcoat 4 is cast for highstrenghtpiston or resin and formed.
Further, described cryostat, 5 is made up of inner wall layer 5-1, heat-insulation layer 5-2 and outer wall layer 5-3.
Further, described centrifuge tube hanger bracket 6 is fixedly linked by the first screw 6-3 and cryostat, 5, and on centrifuge tube hanger bracket, interface 6-2 is higher than the end face of mortar 3, centrifuge tube hanger bracket 6 is provided with grinding pestle suppending hole 6-1.
Further, described grinding pestle hanger bracket 7 is fixedly linked by the second screw 7-1 and cryostat, 5, and on grinding pestle hanger bracket, interface 7-2 is lower than the top of cryostat, 5, and grinding pestle hanger bracket 7 is provided with muller suppending hole 7-3.
Described a kind of liquid nitrogen grinding system, during use, ice cube, liquid nitrogen are added cryostat, or do not add whatever, then mortar frosted plastic overcoat 4 is placed on mortar 3, again test specimen is placed in mortar frosted plastic overcoat 4, subsequently with the manual ground sample repeatedly of grinding pestle 1 with grinding pestle frosted plastic overcoat 2, until by sample grinding thoroughly fully, finally ground sample is transferred in centrifuge tube or test tube.Often grind a test specimen, replaceable mortar frosted plastic overcoat 4 and mortar frosted plastic overcoat 4, to prevent the pollution of sample.This system can quick and convenient broken various test specimen effectively, has advantage that is easy, quick, efficient and low cost, and can not cause the pollution between test specimen.
The foregoing is only preferred embodiment of the present utility model, not in order to limit the utility model, all within spirit of the present utility model and principle, any amendment done, equivalent replacement, improvement etc., all should be included within protection domain of the present utility model.
Claims (8)
1. a liquid nitrogen grinding system, it is characterized in that comprising grinding pestle, described grinding pestle periphery is provided with grinding pestle frosted plastic overcoat, pestle is corresponding is arranged in cryostat, for mortar and grinding, mortar is provided with mortar frosted plastic overcoat, in described cryostat, also correspondence is provided with centrifuge tube hanger bracket and grinding pestle hanger bracket.
2. a kind of liquid nitrogen grinding system as claimed in claim 1, it is characterized in that: it is expand the grinding pestle head of formula structure that described grinding pestle comprises the handle of upper end and lower end, handle is connected by screw thread with grinding pestle head, and grinding pestle head upper end is provided with the scalariform annulus for fixing grinding pestle frosted plastic overcoat.
3. a kind of liquid nitrogen grinding system as claimed in claim 2, is characterized in that: the described grinding pestle head expanding formula structure is for supporting grinding interface grinding pestle frosted plastic overcoat, and described screw thread replaces with hexagonal jack.
4. a kind of liquid nitrogen grinding system as claimed in claim 2, it is characterized in that: described grinding pestle frosted plastic overcoat is frosted process interface with grinding pestle head contact surface, the neck of grinding pestle frosted plastic overcoat is the imperfect ring structures with scalariform annulus corresponding matching.
5. a kind of liquid nitrogen grinding system as claimed in claim 1, it is characterized in that: the upper surface of described mortar is corresponding with grinding pestle head is provided with hole of caving in, grind mouth and projection, mortar bottom is provided with grinding support, and grinding support is connected with cryostat, by fixing rivet; Described mortar frosted plastic overcoat lower surface is corresponding with mortar upper surface is provided with boss, grind muzzle and groove, and described mortar frosted plastic overcoat and mortar contact surface are frosted process interface.
6. a kind of liquid nitrogen grinding system as claimed in claim 1, is characterized in that: described cryostat, is made up of inner wall layer, heat-insulation layer and outer wall layer.
7. a kind of liquid nitrogen grinding system as claimed in claim 1, it is characterized in that: described centrifuge tube hanger bracket is fixedly linked by the first screw and cryostat, on centrifuge tube hanger bracket, interface is higher than the end face of mortar, centrifuge tube hanger bracket is provided with grinding pestle suppending hole.
8. a kind of liquid nitrogen grinding system as claimed in claim 1, it is characterized in that: described grinding pestle hanger bracket is fixedly linked by the second screw and cryostat, on grinding pestle hanger bracket, interface is lower than the top of cryostat, and grinding pestle hanger bracket is provided with muller suppending hole.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201520785874.2U CN205074066U (en) | 2015-10-11 | 2015-10-11 | Liquid nitrogen system of grinding |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201520785874.2U CN205074066U (en) | 2015-10-11 | 2015-10-11 | Liquid nitrogen system of grinding |
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CN205074066U true CN205074066U (en) | 2016-03-09 |
Family
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CN201520785874.2U Expired - Fee Related CN205074066U (en) | 2015-10-11 | 2015-10-11 | Liquid nitrogen system of grinding |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105170280A (en) * | 2015-10-11 | 2015-12-23 | 西北农林科技大学 | Liquid nitrogen grinding system |
-
2015
- 2015-10-11 CN CN201520785874.2U patent/CN205074066U/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105170280A (en) * | 2015-10-11 | 2015-12-23 | 西北农林科技大学 | Liquid nitrogen grinding system |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160309 Termination date: 20171011 |
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CF01 | Termination of patent right due to non-payment of annual fee |