CN205062078U - Quick viral enrichment device of environment water sample of high efficiency counterflush - Google Patents
Quick viral enrichment device of environment water sample of high efficiency counterflush Download PDFInfo
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- CN205062078U CN205062078U CN201520498444.2U CN201520498444U CN205062078U CN 205062078 U CN205062078 U CN 205062078U CN 201520498444 U CN201520498444 U CN 201520498444U CN 205062078 U CN205062078 U CN 205062078U
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- water sample
- counterflush
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Abstract
The utility model discloses a quick viral enrichment device of the environment water sample of high efficiency counterflush, including first, second grade filtering component, laminar flow enrichment subassembly, peristaltic pump and PLC program controller etc. Still be equipped with the counterflush pump. This device has combined to hold back that the formula is filtered, the advantage of two kinds of techniques of slipstream separation, has guaranteed the higher rate of recovery of viral enrichment in bulky, the high flow capacity water sample, introduces efficient back flush link in good time, can prevent effectively that the membrane module of separation of impurities in -process from blockking up, can reliable continuous operation and embody the outstanding feature of its quick viral enrichment. This enrichment installation of automated degree is higher, has quick viral enrichment, continuous operation is reliable, convenient to use is economic outstanding advantage, is particularly useful for on -the -spotly becoming to be used for laboratory detection after the small sample concentrate appearance with big sample water sample is concentrated.
Description
Technical field
The utility model belongs to bioanalysis test and environmental science, particularly relates to a kind of Quick environmental water sample virus enriching device of high-level efficiency counterflush.
Background technology
Notification number CN201681012U utility model " Quick environmental water sample virus enriching device " is a kind of viral enriching apparatus being separated two kinds of technological merits in conjunction with interception type filtration with tangential flow, this device is provided with application of sample bucket, its outlet connects with first step filter assemblies entrance, first step filter assemblies is provided with the first layer coarse filtration film in 100 μm, aperture and the second layer coarse filtration film in 30 μm, aperture, the outlet of first step filter assemblies connects with the 1st two position, three-way electromagnetic change valve P mouth, this reversing valve A mouth connects with second stage filter assemblies entrance, B mouth connects with recoil waste liquid bottle, second stage filter assemblies is provided with the filtration recoil film in 10 μm, aperture and is positioned at the pressure transmitter of outlet, the outlet of second stage filter assemblies connects with the 2nd two position, three-way electromagnetic change valve P mouth, this reversing valve A mouth connects with peristaltic pump Single port, B mouth exports with specimen bottle and connects, peristaltic pump another port connects with the 3rd two position, three-way electromagnetic change valve P mouth, this reversing valve A mouth connects with laminar flow enrichment module inlet, B mouth connects with recoil bottle, laminar flow enrichment module outlet connects with specimen bottle entrance, cleaner liquid outlet is connected with waste liquid bottle, this device is provided with PLC sequence controller, enriching apparatus starts, the pressure transmitter of stop signal and second stage filter assemblies represents that the signal of filter membrane blockage inputs to PLC sequence controller, and the corresponding steering order correspondence that PLC sequence controller exports is connected to the drive element of the 1st, the 2nd, the 3rd two position, three-way electromagnetic change valve and peristaltic pump.
The enrichment flow process of above-mentioned utility model comprises preliminary filtration, viral enrichment two basic steps and counterflush troubleshooting steps.The object of counterflush is the blocking getting rid of second stage filter assemblies generation, the filtration capacity of recovery component, its flow process is: when the filtration recoil film blocking of second stage filter assemblies, the pressure transmitter of its outlet detects pressure anomaly rising, now, PLC sequence controller is by cut out the 1st, the P mouth of the 2nd two position, three-way electromagnetic change valve and the B mouth of the 3rd two position, three-way electromagnetic change valve, open the A of the 2nd two position, three-way electromagnetic change valve, the A of B mouth and the 3rd two position, three-way electromagnetic change valve, P mouth, peristaltic pump flows to and is set as reverse direction flow, the pure water extracted in recoil bottle rinses the filtration recoil film in the filter assemblies of the second stage by this device, flushing waste drains into recoil waste liquid bottle, until the force value that pressure transmitter records recovers normal.
In addition, above-mentioned utility model must be cleaned whole device after each viral enrichment, to avoid the crossed contamination caused between water sample, and microorganism growing in pipeline when preventing long time stored.This cleaning process is also complete under PLC sequence controller controls.Cleaning process is: first in environmental water sample application of sample bucket, inject 100ppm Peracetic Acid 10L, take off the filter membrane of first and second grade of filter assemblies, close the B mouth of the 1st, the 2nd, the 3rd two position, three-way electromagnetic change valve, open A, P mouth of each valve, what set peristaltic pump flows to positive flow, and now scavenging solution fully cleans under the driving of peristaltic pump pipeline.
Practice finds, the counterflush limited pressure that the peristaltic pump of above-mentioned utility model provides, and when particularly cleaning whole device, efficiency is lower.
Summary of the invention
The purpose of this utility model is the technical problem of the counterflush limited pressure that the peristaltic pump overcoming above-mentioned Quick environmental water sample virus enriching device provides, and provides a kind of Quick environmental water sample virus enriching device of high-level efficiency counterflush.
The utility model takes following technical scheme for achieving the above object: the Quick environmental water sample virus enriching device of high-level efficiency counterflush, by application of sample bucket, first step filter assemblies, second stage filter assemblies, 1st two position, three-way electromagnetic change valve, 2nd two position, three-way electromagnetic change valve, 3rd two position, three-way electromagnetic change valve, connect the pressure transmitter of second stage filter assemblies outlet, specimen bottle, recoil bottle, recoil waste liquid bottle, laminar flow enrichment assembly, peristaltic pump and PLC sequence controller formed, feature is, counterflush pump is connected with between the A mouth of described 2nd two position, three-way electromagnetic change valve and the P mouth of the 3rd two position, three-way electromagnetic change valve, the corresponding controling end of PLC sequence controller is connected to the drive element of counterflush pump.
The utility model can also take following technical measures:
In described 1st grade of filter assemblies, the first layer coarse filtration film and second layer coarse filtration film are disk filter membrane.
Filtering recoil film in described 2nd grade of filter assemblies is disk recoil film.
The molecular weight cut-off of described laminar flow enrichment assembly is 60K ~ 80K, and what inside was filled is hollow fiber filtering membrane, its single filter membrane internal-and external diameter 0.9mm/1.3mm, total surface area 0.3m
2.
The beneficial effects of the utility model are with advantage: this device combines the advantage that interception type filters, tangential flow is separated two kinds of technology, ensure that the rate of recovery that in large volume, high flow capacity water sample, viral enrichment is higher, the back flushing link that in good time introducing is main power source with counterflush pump, together with peristaltic pump, high-level efficiency back flushing is carried out to second stage filter assemblies or this device, effectively can prevent that the membrane module in magazins' layout process from blocking, growing and breeding of avoiding the crossed contamination between sample and microorganism in pipeline.This enriching apparatus level of automation is higher, has the outstanding advantages of quick virus enrichment, continuously economy reliable, easy to use, is particularly useful for being used for test in laboratory after large sample water sample is condensed into small sample concentrated solution sample by scene.
Accompanying drawing explanation
Fig. 1 is the utility model virus enriching apparatus example structure schematic diagram.
Fig. 2 is the filling mode schematic diagram of the hollow fiber filter membrane of Fig. 1 laminar flow enrichment assembly.
Fig. 3 is the viral enriching apparatus workflow block diagram of Fig. 1 embodiment.
Fig. 4 is the viral enriching apparatus cleaning process block diagram of Fig. 1 embodiment.
Number in the figure: 1 application of sample bucket, 2 first step filter assemblies, 2-1 the first layer coarse filtration film, 2-2 second layer coarse filtration film, 3 the 1st two position, three-way electromagnetic change valves, 4 second stage filter assemblies, 4-1 filters recoil film, 5 recoil waste liquid bottles, 6 pressure transmitters, 7 the 2nd two position, three-way electromagnetic change valves, 8 peristaltic pumps, 9 counterflush pumps, 10 the 3rd two position, three-way electromagnetic change valves, 11 specimen bottles, 12 recoil bottles, 13 laminar flow enrichment assemblies, 13-1 hollow fiber filtering membrane, 14 waste liquid bottles.
Embodiment
The utility model is further illustrated below in conjunction with embodiment and accompanying drawing thereof.
As shown in Figure 1, in the water sample of the high-level efficiency counterflush of the present embodiment, viral enriching apparatus is provided with application of sample bucket 1, its outlet connects with the entrance of first step filter assemblies 2, first step filter assemblies 2 is provided with the first layer coarse filtration film 2-1 in 100 μm, aperture and the second layer coarse filtration film 2-2 in 30 μm, aperture, the first layer coarse filtration film and second layer coarse filtration film are disk filter membrane, and its diameter is 100mm.
The outlet of first step filter assemblies 2 connects with the P mouth of the 1st two position, three-way electromagnetic change valve 3, and the A mouth of this reversing valve connects with the entrance of second stage filter assemblies 4, and B mouth connects with recoil waste liquid bottle 5.
Be provided with the filtration recoil film 4-1 of disc in second stage filter assemblies 4, its diameter is 30mm, and aperture is 10 μm.The outlet of second stage filter assemblies 4 also arranges pressure transmitter 6, the outlet of second stage filter assemblies simultaneously also connects with the P mouth of the 2nd two position, three-way electromagnetic change valve 7, A mouth and the peristaltic pump 8 of this reversing valve illustrate left side port and connect, and the B mouth of this reversing valve connects with the outlet of specimen bottle 11.
On the right side of the diagram of peristaltic pump 8, port connects with the P mouth of the 3rd two position, three-way electromagnetic change valve 10, and this reversing valve A mouth connects with laminar flow enrichment assembly 13 entrance, and the B mouth of this reversing valve connects with recoil bottle 12.The outlet of laminar flow enrichment assembly 13 connects with the entrance of specimen bottle 11.The cleaner liquid outlet C of laminar flow enrichment assembly 13 is connected with waste liquid bottle 14.
Counterflush pump 9 is connected with between the A mouth of the 2nd two position, three-way electromagnetic change valve 7 and the P mouth of the 3rd two position, three-way electromagnetic change valve 10.
As shown in Figure 2, the outside dimension of laminar flow enrichment assembly is Φ 50mm × 386mm, operating pressure 0.12MPa, and molecular weight cut-off is 60K ~ 80K, and what inside was filled is hollow fiber filtering membrane, its single filter membrane internal-and external diameter 0.9mm/1.3mm, total surface area 0.3m
2.
In embodiment, the latex flexible pipe of the 2nd two position, three-way electromagnetic change valve 7, the 3rd two position, three-way electromagnetic change valve 10 and the connecting pipeline of peristaltic pump 8 corresponding port to be internal diameter be 8mm, other connecting pipeline all adopts internal diameter to be the hard teflon pipe of 5mm ~ 8mm.
This device is provided with PLC sequence controller (not shown), the signal that recoil film 4-1 blockage is filtered in the expression that the pressure transmitter 6 of the startup of enriching apparatus, stop signal and second stage filter assemblies 4 exports inputs to PLC sequence controller, the instruction correspondence of the corresponding controling end that PLC sequence controller exports is connected to the drive element of the 1st, the 2nd, the 3rd two position, three-way electromagnetic change valve, peristaltic pump and counterflush pump 9, and its workflow as shown in Figure 3.The object of this flow process is tentatively to filter it when retaining virus in water sample as far as possible, gets rid of most of impurity.In water sample, viral enrichment flow process comprises preliminary filtration, viral enrichment two basic steps and counterflush step.
Preliminary filtering process is as described in the flow chart of figure 3: first inject in environmental water sample application of sample bucket and treat enrichment water sample, in recoil bottle, add clean water.Then this device switch is started, under PLC time variable control, device closes the B mouth of the 1st, the 2nd and the 3rd two position, three-way electromagnetic change valve, open A, P mouth of the 1st, the 2nd and the 3rd two position, three-way electromagnetic change valve, and the flow direction of peristaltic pump is set as 1-2 positive flow, now, environmental water sample is by under the effect of peristaltic pump, successively through first step filter assemblies, second stage filter assemblies and laminar flow enrichment assembly, filter out impurities and flow into specimen bottle containing virulent water sample, cleaner liquid enters waste liquid bottle.
Virus enrichment flow process is: first close the 1st, the P mouth of the 2nd two position, three-way electromagnetic change valve and the B mouth of the 3rd two position, three-way electromagnetic change valve, open the A of the 2nd two position, three-way electromagnetic change valve simultaneously, the A of P mouth and the 3rd two position, three-way electromagnetic change valve, P mouth, the flow direction of setting peristaltic pump is 1-2 positive flow, now, water sample through coarse filtration and essence filter will no longer through the 1st, cascade filtration assembly, only repeatedly circulate between specimen bottle and laminar flow enrichment assembly, often carry out 1 circulation, all discharge a certain amount of cleaner liquid, until the viral suspension in specimen bottle reaches final target volume, now stop enrichment cycles, virus enrichment flow process completes.
Counterflush flow process is: this flow process object is get rid of blocking, the filtration capacity of recovery component when blocking occurs second stage filter assemblies.
Pressure transmitter is connected with in the outlet of second stage filter assemblies, when the filtration recoil film blocking of this assembly, pressure can raise extremely, this is extremely by pressure transmitter is detected, now, PLG sequence of control is by closedown the 1st, the P mouth of the 2nd two position, three-way electromagnetic change valve and the B mouth of the 3rd two position, three-way electromagnetic change valve, open the A of the 2nd two position, three-way electromagnetic change valve, the A of B mouth and the 3rd two position, three-way electromagnetic change valve, P mouth, start counterflush pump and peristaltic pump, peristaltic pump flows to and is set as 2-1 reverse direction flow, now, the pure water extracted in recoil bottle rinses the filtration recoil film in the filter assemblies of the second stage by this device, flushing waste drains into recoil waste liquid bottle, until the force value that pressure transmitter records recovers normal.
As shown in Figure 4, must clean whole device after viral enrichment in each water sample, to avoid the crossed contamination caused between water sample, and microorganism growing in pipeline when preventing long time stored.This cleaning process automatically completes under PLG sequence of control controls.Cleaning process is: first in environmental water sample application of sample bucket, inject 100ppm Peracetic Acid 10L, take off the filter membrane of first and second grade of filter assemblies, close the B mouth of the 1st, the 2nd, the 3rd two position, three-way electromagnetic change valve, open A, P mouth of each valve, start counterflush pump and peristaltic pump, the flow direction of setting peristaltic pump is 1-2, and now scavenging solution fully cleans under the driving of counterflush pump and peristaltic pump pipeline.
In the water sample of the present embodiment, viral enriching apparatus effect is in the sample good, viral recovery is more than 40%, sample water sample processing power can reach more than 50L/h, and in use substantially occur without line clogging situation, after enrichment, cleaning performance is good, for follow-up enrichment is ready.
Claims (4)
1. the Quick environmental water sample virus enriching device of high-level efficiency counterflush, by application of sample bucket, first step filter assemblies, second stage filter assemblies, 1st two position, three-way electromagnetic change valve, 2nd two position, three-way electromagnetic change valve, 3rd two position, three-way electromagnetic change valve, connect the pressure transmitter of second stage filter assemblies outlet, specimen bottle, recoil bottle, recoil waste liquid bottle, laminar flow enrichment assembly, peristaltic pump and PLC sequence controller formed, it is characterized in that: between the A mouth of described 2nd two position, three-way electromagnetic change valve and the P mouth of the 3rd two position, three-way electromagnetic change valve, be connected with counterflush pump, the corresponding controling end of PLC sequence controller is connected to the drive element of counterflush pump.
2. Quick environmental water sample virus enriching device according to claim 1, is characterized in that: in described first step filter assemblies, the first layer coarse filtration film and second layer coarse filtration film are disk filter membrane.
3. Quick environmental water sample virus enriching device according to claim 1, is characterized in that: filtering recoil film in the filter assemblies of the described second stage is disk recoil film.
4. Quick environmental water sample virus enriching device according to claim 1, it is characterized in that: the molecular weight cut-off of described laminar flow enrichment assembly is 60K ~ 80K, what inside was filled is hollow fiber filtering membrane, its single filter membrane internal-and external diameter 0.9mm/1.3mm, total surface area 0.3m
2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520498444.2U CN205062078U (en) | 2015-07-13 | 2015-07-13 | Quick viral enrichment device of environment water sample of high efficiency counterflush |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520498444.2U CN205062078U (en) | 2015-07-13 | 2015-07-13 | Quick viral enrichment device of environment water sample of high efficiency counterflush |
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| CN205062078U true CN205062078U (en) | 2016-03-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520498444.2U Expired - Fee Related CN205062078U (en) | 2015-07-13 | 2015-07-13 | Quick viral enrichment device of environment water sample of high efficiency counterflush |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110286000A (en) * | 2019-07-16 | 2019-09-27 | 中国科学院海洋研究所 | A kind of fluid sample Suction filtration device and its application method based on ROV |
-
2015
- 2015-07-13 CN CN201520498444.2U patent/CN205062078U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110286000A (en) * | 2019-07-16 | 2019-09-27 | 中国科学院海洋研究所 | A kind of fluid sample Suction filtration device and its application method based on ROV |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 300171, Hedong Road, Hedong District, Tianjin, No. third, floor 316, room 66, eleven Patentee after: TIANJIN HONOUR INSTRUMENT CO., LTD. Address before: 300171, Hedong Road, Hedong District, Tianjin, No. third, floor 316, room 66, eleven Patentee before: Tianjin Ounuo Instrument Co., Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160302 Termination date: 20190713 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |