CN205038161U - Basement of flower type silver nanoparticle enhanced fluorescence - Google Patents
Basement of flower type silver nanoparticle enhanced fluorescence Download PDFInfo
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- CN205038161U CN205038161U CN201520648538.3U CN201520648538U CN205038161U CN 205038161 U CN205038161 U CN 205038161U CN 201520648538 U CN201520648538 U CN 201520648538U CN 205038161 U CN205038161 U CN 205038161U
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Abstract
The utility model discloses a basement of flower type silver nanoparticle enhanced fluorescence, which comprises a substrate, be provided with colored type silver nanoparticle layer on the substrate, the inferior single layer structure that flower type silver nanoparticle layer is constituteed for a plurality of flower type silver nanoparticle. Flower type silver nanoparticle layer is that the coverage rate is 40% - 80% inferior single layer structure. Wherein, flower type silver nanoparticle particle diameter is 0.5 -3 mu m, then single it is coarse that flower type silver nanoparticle surface has roughness 5 -300nm's nanometer. The nanometer rough structure that utilizes flower type silver nanoparticle surface enlargies the the fluorescence signal of molecule transmission, the magnification 10 -100 doubly, owing to do not need extra operation to enlarge, this technique is also convenient than the conventional art, simple simultaneously.
Description
Technical field
The utility model relates to fluorescence analysis, and more particularly it is the substrate of a kind of flower pattern silver nano-grain Fluorescence Increasing.
Background technology
Fluorescence analysis is a kind of important detection method at biological, medical field, and main technological means is ELISA.It utilizes biomolecule if the biologic specificity identification of antigen, antibody etc. is to detect specific molecule, and then the diagnosis state of an illness.But this technology has certain limitation in the micro-biomolecule of detection.Usually at the state of an illness initial stage, antibody equimolecular is less, and required detection time needs several days usually, even several weeks, greatly delays condition-inference.And diagnosis not in time makes result for the treatment of reduce usually, available treatment means reduces, and this situation is extensively present in such as type 1 diabetes, and cancer, in the diagnosis of acquired immune deficiency syndrome (AIDS) etc.
In recent years research finds, by mating surface plasmon technology, greatly can strengthen the fluorescence signal intensity of molecule, the highest enhancing efficiency can reach 10000 times.If this technology is applied to ELISA, can make greatly shorten detection time, from several days, a few week foreshorten to several hours, this provides possibility for the timely diagnosis state of an illness, accepts more effective treatment very crucial to patient.
Surface plasmons strengthens fluorescent technique to be needed specific substrate to realize.Amplified medium in these substrates is normally made up of metal Nano structures such as gold, silver, strengthens the fluorescence signal around this region by the coupling (forming nanometer gap) between nanostructured.
Conventional enhancing substrate has two kinds, and a kind of is the regular nanostructured utilizing micro-nano manufacturing process to prepare, and be characterized in that signal relative deviation is little, signal reproducibility is high, but signal strengthens relatively little.Simultaneously owing to have employed micro-nano process technology, strengthening region can process arbitrarily in substrate desired position as requested, finds the time of determinand position, reduce the difficulty of operation when shortening detection.But on the other hand, needed for micro-processing technology, cost is huge, needs dust free room and related processing equipment.
And another kind of substrate spreads on smooth substrate by the nano particle of chemosynthesis, the feature of this substrate is that signal enhancing is high, but each deviation of signal gathered is comparatively large, and reappearance is lower.Operationally, because conventional nano-particles size is in tens to hundreds of nanometer, usually will allows between particle and be polymerized, could produce and strengthen signal, and the process of polymerization is often difficult to control, and is difficult to reach optimal conditions.Conventional solution time particle is modified or is grown at substrate surface, but the basal signal obtained like this strengthens limited.
Utility model content
The flower pattern silver nano-grain Fluorescence Increasing substrate that the purpose of this utility model is to overcome existing two kinds of Fluorescence Increasing technical deficiency parts and provides.
The utility model realizes by following technical scheme:
The substrate of flower pattern silver nano-grain Fluorescence Increasing, comprises substrate, described substrate is provided with flower pattern Silver nano-particle layer, and described flower pattern Silver nano-particle layer is the subband structures structure of several flower pattern silver nano-grains composition.
Described flower pattern Silver nano-particle layer to be coverage rate be 40%-80% subband structures structure.
Wherein, described flower pattern silver nano-grain particle diameter is 0.5-1 μm, then single described flower pattern silver nano-grain surface has the nanoscale rough of roughness 5-10nm.
When described flower pattern silver nano-grain particle diameter is 1-2 μm, single described flower pattern silver nano-grain surface has the nanoscale rough of roughness 10-100nm.
Selection particle diameter is the described flower pattern silver nano-grain of 2-3 μm, and now single described flower pattern silver nano-grain surface has the nanoscale rough of roughness 100-300nm.
Compared with existing fluorescence analysis detection technique medically, this technology for detection efficiency is high, consuming time few.Conventional analytical techniques is after a small amount of antibody or antigen molecule are captured, and often can not detect immediately, this is mainly because instrument and equipment cannot detect so faint signal.Usual solution utilizes amplifying technique, such as biotin-Streptavidin iodine, and this technical requirement is high, needs testing staff through professional training, length consuming time, and signal amplifying speed rate is slow.This technology utilizes surface plasma enhancing technology, the fluorescence signal of nanoscale rough structure to molecular emission on flower pattern silver nano-grain surface is utilized to amplify, enlargement factor at 10-100 doubly, simultaneously owing to not needing extra operation to amplify, also comparatively conventional art is convenient, simple for this technology.
Accompanying drawing explanation
Fig. 1 is that the substrate of flower pattern silver nano-grain Fluorescence Increasing uses structural representation.
Fig. 2 is flower pattern silver nano-grain structure for amplifying schematic diagram
In figure: substrate 1 (glass sheet or silicon chip); Flower pattern Silver nano-particle layer 2; Fluorescent biomolecules 3 to be measured; Is furnished with the microscope 4 of CCD; Display screen 5; For exciting light/reception light signal circuit 6.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail, but this embodiment should not be construed restriction of the present utility model, only for example, simultaneously by illustrating that advantage of the present utility model will become clearly and easy understand.
The substrate of flower pattern silver nano-grain Fluorescence Increasing, comprises substrate 1, substrate 1 is provided with flower pattern Silver nano-particle layer 2, and flower pattern Silver nano-particle layer 2 is the subband structures structure of several flower pattern silver nano-grains composition.
In flower pattern Silver nano-particle layer 2, flower pattern silver nano-grain arranges accumulation on substrate 1 with the form of Hexagonal packing, if this accumulation does not have point defect or line defect in substrate 1 scope, is called full individual layer, and namely coverage rate is 100%.
Preferably, flower pattern Silver nano-particle layer 2 to be coverage rates be 40%-80% subband structures structure.
Preferably, flower pattern silver nano-grain particle diameter is 0.5-1 μm, and single flower pattern silver nano-grain surface has the nanoscale rough of roughness 5-10nm, the gully namely in Fig. 2 on single flower pattern silver nano-grain.
Preferably, flower pattern silver nano-grain particle diameter is 1-2 μm, and single flower pattern silver nano-grain surface has the nanoscale rough of roughness 10-100nm.
Preferably, flower pattern silver nano-grain particle diameter is 2-3 μm, and single flower pattern silver nano-grain surface has the nanoscale rough of roughness 100-300nm.
During utility model works, fluorescence molecule 3 to be measured is adsorbed on flower pattern Silver nano-particle layer 2.Be provided with the microscope 4 with CCD above substrate 1, the microscope 4 with CCD is connected with display screen 5.
It should be noted that: to those of ordinary skill in the art, can also make some changes or distortion to the utility model under the prerequisite not changing the utility model principle, this belongs to protection domain of the present utility model equally.
Claims (5)
1. flower pattern silver nano-grain Fluorescence Increasing substrate, comprise substrate (1), it is characterized in that: described substrate (1) is provided with flower pattern Silver nano-particle layer (2), the subband structures structure that described flower pattern Silver nano-particle layer (2) forms for several flower pattern silver nano-grains.
2. flower pattern silver nano-grain Fluorescence Increasing according to claim 1 substrate, is characterized in that: described flower pattern Silver nano-particle layer (2) to be coverage rate be 40%-80% subband structures structure.
3. flower pattern silver nano-grain Fluorescence Increasing according to claim 2 substrate, is characterized in that: described flower pattern silver nano-grain particle diameter is 0.5-1 μm, and single described flower pattern silver nano-grain surface has the nanoscale rough of roughness 5-10nm.
4. flower pattern silver nano-grain Fluorescence Increasing according to claim 2 substrate, is characterized in that: described flower pattern silver nano-grain particle diameter is 1-2 μm, and single described flower pattern silver nano-grain surface has the nanoscale rough of roughness 10-100nm.
5. flower pattern silver nano-grain Fluorescence Increasing according to claim 2 substrate, is characterized in that: described flower pattern silver nano-grain particle diameter is 2-3 μm, and single described flower pattern silver nano-grain surface has the nanoscale rough of roughness 100-300nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520648538.3U CN205038161U (en) | 2015-08-25 | 2015-08-25 | Basement of flower type silver nanoparticle enhanced fluorescence |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520648538.3U CN205038161U (en) | 2015-08-25 | 2015-08-25 | Basement of flower type silver nanoparticle enhanced fluorescence |
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| CN205038161U true CN205038161U (en) | 2016-02-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520648538.3U Expired - Fee Related CN205038161U (en) | 2015-08-25 | 2015-08-25 | Basement of flower type silver nanoparticle enhanced fluorescence |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105136757A (en) * | 2015-08-25 | 2015-12-09 | 武汉奥旷精密仪器有限公司 | Flower-shaped silver nanometer particle fluorescence-enhanced substrate and preparation method thereof |
| CN108436253A (en) * | 2018-02-26 | 2018-08-24 | 北京航空航天大学 | A kind of preparation method of SERS- fluorescent dual modules formula metal enhancing substrate |
-
2015
- 2015-08-25 CN CN201520648538.3U patent/CN205038161U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105136757A (en) * | 2015-08-25 | 2015-12-09 | 武汉奥旷精密仪器有限公司 | Flower-shaped silver nanometer particle fluorescence-enhanced substrate and preparation method thereof |
| CN108436253A (en) * | 2018-02-26 | 2018-08-24 | 北京航空航天大学 | A kind of preparation method of SERS- fluorescent dual modules formula metal enhancing substrate |
| CN108436253B (en) * | 2018-02-26 | 2020-07-17 | 北京航空航天大学 | Preparation method of SERS-fluorescence dual-mode metal enhanced substrate |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160217 Termination date: 20200825 |