CN204981871U - Novel lymphocyte separator tube - Google Patents

Novel lymphocyte separator tube Download PDF

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Publication number
CN204981871U
CN204981871U CN201520762025.5U CN201520762025U CN204981871U CN 204981871 U CN204981871 U CN 204981871U CN 201520762025 U CN201520762025 U CN 201520762025U CN 204981871 U CN204981871 U CN 204981871U
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China
Prior art keywords
plug
unit
components
pipe according
lymphocyte
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CN201520762025.5U
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Chinese (zh)
Inventor
程吉频
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Zhejiang Baidi Biotechnology Co., Ltd
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Hangzhou Biocode Technology Co Ltd
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Priority to CN201520762025.5U priority Critical patent/CN204981871U/en
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Abstract

The utility model discloses a novel lymphocyte separator tube, including a pipe body, the body is the tube -shape, and the lower part of tube -shape is for centrum form, and be located the body and be provided with plug -in components, plug -in components comprise a cylinder structure and a concave surface structure integrated into one piece, the concave surface structure is located the lower part fretwork of the upper portion of cylinder structure, cylinder structure, be located still be provided with the cavernous structure that a plurality of is used for separating lymphocyte on the plug -in components. Add the lymphocyte parting liquid below the plug -in components then add the sample more than plug -in components, can directly make the layering of target lymphocyte in the plug -in components top through the centrifugation, with separation of impurities in the plug -in components below, take lymphocyte direct just can pour can, can not spill below the plug -in components. Its operation is simpler, and the impurity that the error results in when reducing the rifle head and drawing, and is purer than ordinary separation.

Description

Novel separation of lymphocytes pipe
Technical field
The utility model relates to a kind of cell Separating tube, particularly relates to a kind of separation of lymphocytes pipe, belongs to bio-medical technology field.
Background technology
Only have common separation of lymphocytes pipe in the market, its method is by adding sample and lymphocyte separation medium carries out centrifugal, can layering in centrifugal rear separator tube, and then experimenter needs to draw target lymphocyte with rifle head.Its shortcoming is exactly easy suck up impurity in suction process, operate cumbersome, not only waste time and energy, and error is large.
Utility model content
For solving the problems of the technologies described above, the utility model proposes a kind of simple to operation and be separated pure novel separation of lymphocytes pipe.
In order to achieve the above object, the technical solution of the utility model is as follows: the utility model devises a kind of plug-in application of uniqueness in cell Separating tube, and it can prevent density gradient centrifugation liquid and blood sample from mixing.The mid-way of this plug-in unit is provided with a center sorting hole, and use transfer pipet that density gradient centrifugation liquid is added bottom by center sorting hole, sample is added in the top of plug-in unit along tube wall, so just need not cautiously be added in above density gradient centrifugation liquid.Can pour in new centrifuge tube by blood plasma and lymphocyte easily after centrifugal, impurity be stayed bottom plug-in unit, saves operating time and operate miss.Its concrete scheme is as follows:
A kind of novel separation of lymphocytes pipe, comprise body, described body is tubular, and the bottom of tubular is centrum shape, be positioned at body and be provided with a plug-in unit, described plug-in unit is by a cylindrical structure and a concave structure is one-body molded forms, and described concave structure is positioned at the top of cylindrical structure, the bottom hollow out of cylindrical structure; Be positioned on described plug-in unit and be also provided with several for separating of lymphocytic cavernous structure.
As preferably, the center being positioned at described concave structure is provided with a center sorting hole, the diameter of described center sorting hole is 1.8mm ~ 2.3mm, lymphocyte separation medium is positioned at the below of plug-in unit, the pyramidal portion of body, is blood above plug-in unit, blood is gone down by center sorting hole in centrifugal process, meanwhile, lymphocyte separation medium is expressed to above plug-in unit from the cavernous structure of plug-in unit, is so just easy to separation of lymphocytes to come.
As preferably, the edge of described concave structure also offers several U-type groove holes uniformly, the rounding diameter in described U-type groove hole is 0.8mm ~ 1.2mm, and the opening in several U-type groove holes all outwardly, the number in described U-type groove hole is three, when when carrying out separation of lymphocytes, the blood sample above plug-in unit and lymph parting liquid is facilitated to carry out exchange of substance.
As preferably, the bottom of described cylindrical structure offers several arcuate sockets uniformly, and the arc degree of depth of described arcuate socket is 1mm, and width is 4mm, and the number of arcuate socket is three, the setting of arcuate socket is used for the sample of centrifuge tube when centrifugal above lymphocyte separation medium and plug-in unit and exchanges.
As preferably, the diameter 1mm larger than the diameter of cylindrical structure of described concave structure, and the inwall of body is close at the edge of concave structure, by larger than the diameter of cylindrical structure for the diameter design of concave structure 1mm, its effect is, also form aperture between such U-type groove hole and body, in centrifugal, lymphocyte separation medium can be split into above plug-in unit by these apertures.
As preferably, the opening in described U-type groove hole contacts with cylindrical structure, and forms a waist-shaped hole.
As preferably, be provided with several stiffening webs in described plug-in unit, the setting of stiffening web serves effect that is stable and that reinforce.
The beneficial effects of the utility model: lymphocyte separation medium is added below plug-in unit and then adds sample more than plug-in unit, can directly make the layering of target lymphocyte above plug-in unit through centrifugal, by magazins' layout below plug-in unit, take lymphocyte directly just can pour out, can not spill below plug-in unit.Its operation is simpler, and minimizing rifle head draws the impurity that time error causes, purer than common separation.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the structural representation of the utility model plug-in unit;
Wherein: 1. body, 2. plug-in unit, 3. cylindrical structure, 4. concave structure, 5. center sorting hole, 6.U type slotted eye, 7. arcuate socket, 8. stiffening web.
Embodiment
Below in conjunction with embodiment, the utility model is described in further detail.
As depicted in figs. 1 and 2, a kind of novel separation of lymphocytes pipe, comprise body 1, described body 1 is tubular, and the bottom of tubular is centrum shape, is positioned at body 1 and is provided with a plug-in unit 2, described plug-in unit 2 is by a cylindrical structure 3 and a concave structure 4 is one-body molded forms, described concave structure 4 is positioned at the top of cylindrical structure 3, the bottom hollow out of cylindrical structure 3; Be positioned on described plug-in unit 2 and be also provided with several for separating of lymphocytic cavernous structure.
The center being positioned at described concave structure 4 is provided with a center sorting hole 5, the diameter of described center sorting hole 5 is 1.8mm ~ 2.3mm, the preferred 2mm of the present embodiment, as long as again can within the scope of this, lymphocyte separation medium is positioned at the below of plug-in unit 2, the pyramidal portion of body 1, plug-in unit 2 is blood above, blood is gone down by center sorting hole 5 in centrifugal process, meanwhile, lymphocyte separation medium is expressed to above plug-in unit 2 from the cavernous structure of plug-in unit 2, is so just easy to separation of lymphocytes to come.
The edge of described concave structure 4 also offers several U-type groove holes 6 uniformly, described U-type groove hole, the rounding diameter of 6 is 0.8mm ~ 1.2mm, preferred 1mm in the present embodiment, as long as certainly within the scope of this, can, and the opening in several U-type groove holes 6 all outwardly, the number in described U-type groove hole 6 is three, when when carrying out separation of lymphocytes, facilitates the blood sample on plug-in unit 2 and lymphocyte separation medium to carry out exchange of substance.
The bottom of described cylindrical structure 3 offers several arcuate sockets 7 uniformly, the arc degree of depth of described arcuate socket 7 is 1mm, width is 4mm, and the number of arcuate socket 7 is three, and the setting of arcuate socket 7 is used for the sample of centrifuge tube when centrifugal above parting liquid and plug-in unit 2 and exchanges.
The diameter 1mm larger than the diameter of cylindrical structure 3 of described concave structure 4, and the inwall of body is close at the edge of concave structure 4, like this, shifting phenomena would not be there is easily in plug-in unit 2 in body 1, by larger than the diameter of cylindrical structure 3 for the diameter design of concave structure 4 1mm, its effect also forms aperture between such U-type groove hole 6 and body 1, in centrifugal, lymphocyte separation medium can be split into above plug-in unit by these apertures.
The opening in described U-type groove hole 6 contacts with cylindrical structure 3, and forms a waist-shaped hole.
Be provided with several stiffening webs 8 in described plug-in unit 2, the setting of stiffening web 8 serves effect that is stable and that reinforce.
The beneficial effects of the utility model: lymphocyte separation medium is added below plug-in unit and then adds sample more than plug-in unit, can directly make the layering of target lymphocyte above plug-in unit through centrifugal, by magazins' layout below plug-in unit, take lymphocyte directly just can pour out just can, can not spill below plug-in unit.Its operation is simpler, and minimizing rifle head draws the impurity that time error causes, purer than common separation, is separated quicker, centrifugal 10 minutes; And without the need to closing whizzer brake.
Its centrifugal method is:
(1), when using, lymphocyte separation medium is joined below plug-in unit 2 by center sorting hole, the pyramidal portion of body 1;
(2) then, add blood sample from the edge of body 1, blood sample is positioned at the top of plug-in unit 2;
(3) then carry out centrifugal, centrifugal rear lymphocyte layering is above plug-in unit 2, and magazins' layout is below plug-in unit 2;
(4) when sampling by separator tube conversely, center sorting hole is just on the lofty perch of plug-in unit 2, and add that the liquid in the plug-in unit 2 of capillary effect the inside cannot be poured out, target substance is above poured out.So just can well by separation of lymphocytes out!

Claims (9)

1. a novel separation of lymphocytes pipe, comprise body (1), described body (1) is tubular, and the bottom of tubular is centrum shape, it is characterized in that, be positioned at body (1) and be provided with a plug-in unit (2), described plug-in unit (2) is made up of with a concave structure (4) is one-body molded a cylindrical structure (3), described concave structure (4) is positioned at the top of cylindrical structure (3), the bottom hollow out of cylindrical structure (3); Be positioned on described plug-in unit (2) and be also provided with several for separating of lymphocytic cavernous structure.
2. novel separation of lymphocytes pipe according to claim 1, it is characterized in that, the center being positioned at described concave structure (4) is provided with a center sorting hole (5), and the diameter at described center sorting hole (5) is 1.8mm ~ 2.3mm.
3. novel separation of lymphocytes pipe according to claim 2, it is characterized in that, the edge of described concave structure (4) also offers uniformly several U-type groove holes (6), and the opening in several U-type groove holes (6) all outwardly, the rounding diameter of described U-type groove hole (6) is 0.8mm ~ 1.2mm.
4. novel separation of lymphocytes pipe according to claim 3, is characterized in that, the number of described U-type groove hole (6) is three.
5. novel separation of lymphocytes pipe according to claim 4, it is characterized in that, the bottom of described cylindrical structure (3) offers several arcuate sockets (7) uniformly, and the arc degree of depth of described arcuate socket (7) is 1mm, and width is 4mm.
6. novel separation of lymphocytes pipe according to claim 5, is characterized in that, the number of described arcuate socket (7) is three.
7. the novel separation of lymphocytes pipe according to any one of claim 1-6, it is characterized in that, the diameter 1mm larger than the diameter of cylindrical structure (3) of described concave structure (4), and the inwall of body (1) is close at the edge of concave structure (4).
8. novel separation of lymphocytes pipe according to claim 7, is characterized in that, the opening of described U-type groove hole (6) contacts with cylindrical structure (3), and forms a waist-shaped hole.
9. novel separation of lymphocytes pipe according to claim 7, is characterized in that, is provided with several stiffening webs (8) in described plug-in unit (2).
CN201520762025.5U 2015-09-29 2015-09-29 Novel lymphocyte separator tube Active CN204981871U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201520762025.5U CN204981871U (en) 2015-09-29 2015-09-29 Novel lymphocyte separator tube

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201520762025.5U CN204981871U (en) 2015-09-29 2015-09-29 Novel lymphocyte separator tube

Publications (1)

Publication Number Publication Date
CN204981871U true CN204981871U (en) 2016-01-20

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105132277A (en) * 2015-09-29 2015-12-09 杭州科鼎生物科技有限公司 Novel lymphocyte separation tube

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105132277A (en) * 2015-09-29 2015-12-09 杭州科鼎生物科技有限公司 Novel lymphocyte separation tube
CN105132277B (en) * 2015-09-29 2018-03-09 杭州科鼎生物科技有限公司 A kind of new separation of lymphocytes pipe

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Effective date of registration: 20191227

Address after: 310051 No.88 Jiangling Road, Xixing street, Binjiang District, Hangzhou City, Zhejiang Province

Patentee after: Zhejiang Baidi Biotechnology Co., Ltd

Address before: 310000 Zhejiang District of Xihu District city of Hangzhou province Shengyuan gudun road 6-503

Patentee before: HANGZHOU BIOCODE TECHNOLOGY CO., LTD.