CN204903478U - Preceding processing apparatus that nitrosamine mark detected - Google Patents
Preceding processing apparatus that nitrosamine mark detected Download PDFInfo
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- CN204903478U CN204903478U CN201520621718.2U CN201520621718U CN204903478U CN 204903478 U CN204903478 U CN 204903478U CN 201520621718 U CN201520621718 U CN 201520621718U CN 204903478 U CN204903478 U CN 204903478U
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- nitrosamine
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- storage pipe
- decontaminating column
- pipe
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Abstract
The utility model relates to a preceding processing apparatus that nitrosamine mark detected. The device by liquid storage pipe, glass piston, rubber stopper, governing valve, decontaminating column, mount, connect liquid bottle and base etc. To constitute, the decontaminating column is connected to the liquid storage pipe, sets up the governing valve between liquid storage pipe and decontaminating column, and the mount is connected to the decontaminating column, and the decontaminating column lower part sets up and connects the liquid bottle, connects the liquid bottle fixed by the base, has a ramuscule pipe on the liquid storage pipe, has the rubber stopper on the ramuscule pipe. Upper portion is responsible for to the liquid storage pipe has glass piston, size and liquid storage pipe pipe diameter phase -match. There is the aperture on the governing valve for adjust the eluate velocity of flow. The decontaminating column packs to nitrosamine molecular engram material. Connect liquid bottle body for circular, the bottle end is the point end, and the bottleneck is the ground, is connected with rotary evaporato. The utility model is used for the measuring of nitrosamine in the urine has advantages such as quick, accuracy.
Description
Technical field
The utility model belongs to technical field of analytical chemistry, is specifically related to a kind of pretreating device being exclusively used in nitrosamine marker detection in urine.
Background technology
Nitrosamine is the compound that the nitrogen-atoms of nitroso--NO is connected with the nitrogen-atoms in amino, is one of four large food contaminants.All nitrosamine is contained in food, cosmetics, beer.In smoked cured food, containing a large amount of nitrosamines materials, some digestive system tumor, the incidence of disease as the cancer of the esophagus is relevant to the nitrosamine quantity taken in meals.When smoked cured food and wine are taken in jointly, nitrosamine will be multiplied to the harm of health.
The character of health risk and degree when research for biomarker is conducive to illustrating that the mankind are exposed to this type of material.Because nitrosamine biomarker content is lower, during detection, interference is comparatively large, higher to the requirement of sample pre-treatments, therefore, develop a kind of be exclusively used in nitrosamine marker detection in urine pretreating device and using method particularly important.
Summary of the invention
The utility model provide nitrosamine marker detection in a kind of urine pretreating device and, for the detection of nitrosamine in urine, there is the advantages such as quick, accurate.
The technical scheme that the utility model adopts is: in a kind of urine the pretreating device of nitrosamine marker detection by liquid storage pipe, variable valve, decontaminating column, fixed mount, connect liquid bottle and base is formed; Liquid storage pipe connects decontaminating column, arranges variable valve, decontaminating column Connection Bracket between liquid storage pipe and decontaminating column, and decontaminating column bottom arranges and connects liquid bottle, connects liquid bottle and is fixed by base, liquid storage pipe has a ramuscule pipe, ramuscule pipe has rubber stopper.
Glass piston is arranged at described liquid storage pipe supervisor top, and size and liquid storage pipe caliber match.
Described variable valve there is aperture, adjustable eluent flow rate.
Described decontaminating column filler is nitrosamine molecular engram material.
Described connects liquid bottle body for circular, and be the point end at the bottom of bottle, bottleneck is ground, can be connected with Rotary Evaporators.
Described base is foam, for fixedly connecing liquid bottle, in seat shape with connect liquid bottle and match.
The utility model provides the pretreating device of nitrosamine marker detection in a kind of urine, for in urine during nitrosamine marker detection, step is simple, sample introduction analysis is got final product after concentrated, for one-stop pretreating device, good purification, highly sensitive, when greatly reducing instrumental analysis, impurity is to the interference of object chromatographic peak; The material that Available experimental room is easy to get is prepared from, and cost is lower, while raising precision of analysis, decreases the pollution of pretreatment process to environment.
Accompanying drawing explanation
Fig. 1 is the pretreating device structural representation of nitrosamine marker detection in the utility model urine.
In figure: 1-glass piston, 2-liquid storage pipe, 3-rubber stopper, 4-variable valve, 5-decontaminating column, 6-fixed mount, 7-connects liquid bottle, 8-base.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in further detail.
Embodiment 1:
As shown in Figure 1, in urine of the present utility model, the pretreating device of nitrosamine marker detection is by glass piston 1, liquid storage pipe 2, rubber stopper 3, variable valve 4, decontaminating column 5, fixed mount 6, connects liquid bottle 7 and base 8 is formed; Liquid storage pipe connects decontaminating column, arranges variable valve, decontaminating column Connection Bracket between liquid storage pipe and decontaminating column, and decontaminating column bottom arranges and connects liquid bottle, connects liquid bottle and is fixed by base, liquid storage pipe has a ramuscule pipe, ramuscule pipe has rubber stopper.
Glass piston 1 can take off, and size and liquid storage pipe caliber 2 match, and liquid storage pipe 2 material is glass, can store up eluent 10mL; Rubber stopper 3, material is rubber, coordinates with liquid storage pipe 2 bottom tubule upwards, after pushing away the solution in clean post 5, prevents the material in decontaminating column 5 to be damaged due to negative pressure when opening glass piston 1; Variable valve 4 material is glass, and valve has aperture, adjustable eluent flow rate, and size and liquid storage pipe 2 match, good airproof performance; Decontaminating column 5, filler is nitrosamine molecular engram material, and specification is 50mg; Fixed mount 6, Main Function is fixing decontaminating column 5; Connect liquid bottle 7, material is glass, and body is circular, and be the point end at the bottom of bottle, bottleneck is ground, can be connected with Rotary Evaporators, and specification is 10mL; Base 8, material is foam, Main Function for fixedly to connect liquid bottle 7, size with connect liquid bottle 7 and match.
In urine, the application of the pretreating device of nitrosamine marker detection is as follows:
Open glass piston 1, get the urine after 3mL enzymolysis in liquid storage pipe, add the inner mark solution (the deuterated N'-nitrosonornicotine of interior mark NNN-d4() of 50 μ L, NAB-d4(deuterated N-nitroso-anabasine), NAT-d4(deuterated N-nitrosoanabasine) and NNAL-d3 [deuterated 4-(N-methyl-N-nitrosamino-)-4-(3-pyridine radicals)-butanols] mixing, concentration is 0.1 ~ 0.2ng/mL, solvent is acetonitrile, the mass ratio of 4 kinds of internal standard compound matter is 1:1:1:2), rotate variable valve 4, sample is about 0.5mL/min to the rear coutroi velocity of decontaminating column 5, leave standstill 5 minutes, add 1mL10mM ammonium acetate solution and flow into decontaminating column 5 through liquid storage pipe 2, open variable valve 4 completely subsequently, cover tightly rubber stopper 3, cover glass piston 1 and slowly push away downwards, the moisture on decontaminating column 5 is pushed away dry, open rubber stopper 3, open glass piston 1, get 1mL normal heptane in liquid storage pipe 2, rotate variable valve 4, drip washing decontaminating column 5, then open variable valve 4 completely, cover tightly rubber stopper 3, cover glass piston 1 and slowly push away downwards, the normal heptane on decontaminating column 5 is pushed away dry completely, finally open rubber stopper 3, open glass piston 1, get 1mL methyl alcohol: the solution of methylene chloride 1:9 (volume ratio) flows into decontaminating column 5 through liquid storage pipe 2 and carries out wash-out, repeat 2 times, eluent is collected with the liquid bottle 7 that connects on base 8, take off and connect liquid bottle 7, redissolve with 150 μ L acetonitriles after evaporate to dryness, enter LC-MS/MS(liquid chromatography-tandem mass spectrum combined instrument) analyze.The utility model is used for the detection of nitrosamine mark in urine, has the advantages such as quick, accurate.
Claims (6)
1. a pretreating device for nitrosamine marker detection, is characterized in that by liquid storage pipe, variable valve, decontaminating column, fixed mount, connects liquid bottle and base is formed; Liquid storage pipe connects decontaminating column, arranges variable valve, decontaminating column Connection Bracket between liquid storage pipe and decontaminating column, and decontaminating column bottom arranges and connects liquid bottle, connects liquid bottle and is fixed by base, liquid storage pipe has a ramuscule pipe, ramuscule pipe has rubber stopper.
2. the pretreating device of nitrosamine marker detection according to claim 1, it is characterized in that glass piston is arranged at described liquid storage pipe supervisor top, size and liquid storage pipe caliber match.
3. the pretreating device of nitrosamine marker detection according to claim 1, is characterized in that described variable valve has aperture, for regulating eluent flow rate.
4. the pretreating device of nitrosamine marker detection according to claim 1, is characterized in that described decontaminating column filler is nitrosamine molecular engram material.
5. the pretreating device of nitrosamine marker detection according to claim 1, it is characterized in that the described liquid bottle body that connects is circular, be the point end at the bottom of bottle, bottleneck is ground, is connected with Rotary Evaporators.
6. the pretreating device of nitrosamine marker detection according to claim 1, is characterized in that described base is foam, for fixedly connecing liquid bottle, in seat shape with connect liquid bottle and match.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520621718.2U CN204903478U (en) | 2015-08-18 | 2015-08-18 | Preceding processing apparatus that nitrosamine mark detected |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520621718.2U CN204903478U (en) | 2015-08-18 | 2015-08-18 | Preceding processing apparatus that nitrosamine mark detected |
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| Publication Number | Publication Date |
|---|---|
| CN204903478U true CN204903478U (en) | 2015-12-23 |
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| CN201520621718.2U Active CN204903478U (en) | 2015-08-18 | 2015-08-18 | Preceding processing apparatus that nitrosamine mark detected |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044252A (en) * | 2015-08-18 | 2015-11-11 | 云南中烟工业有限责任公司 | Pretreatment device for detection of nitrosamine marker and application thereof |
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2015
- 2015-08-18 CN CN201520621718.2U patent/CN204903478U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044252A (en) * | 2015-08-18 | 2015-11-11 | 云南中烟工业有限责任公司 | Pretreatment device for detection of nitrosamine marker and application thereof |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |