CN204789459U - Ethidium bromide adsorption equipment - Google Patents
Ethidium bromide adsorption equipment Download PDFInfo
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- CN204789459U CN204789459U CN201520501975.2U CN201520501975U CN204789459U CN 204789459 U CN204789459 U CN 204789459U CN 201520501975 U CN201520501975 U CN 201520501975U CN 204789459 U CN204789459 U CN 204789459U
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- ethidium bromide
- adsorbent equipment
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- 238000001179 sorption measurement Methods 0.000 title claims abstract description 29
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 title claims abstract description 27
- 229960005542 ethidium bromide Drugs 0.000 title claims abstract description 27
- 238000009833 condensation Methods 0.000 claims abstract description 46
- 230000005494 condensation Effects 0.000 claims abstract description 44
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000012528 membrane Substances 0.000 claims abstract description 5
- 239000003463 adsorbent Substances 0.000 claims description 25
- 238000001962 electrophoresis Methods 0.000 claims description 17
- 229910000838 Al alloy Inorganic materials 0.000 claims description 3
- 239000000956 alloy Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 16
- 108020004707 nucleic acids Proteins 0.000 abstract description 10
- 150000007523 nucleic acids Chemical class 0.000 abstract description 10
- 102000039446 nucleic acids Human genes 0.000 abstract description 10
- 238000001502 gel electrophoresis Methods 0.000 abstract description 9
- 238000012360 testing method Methods 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 4
- 238000009510 drug design Methods 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 239000011543 agarose gel Substances 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000000499 gel Substances 0.000 description 22
- 239000003292 glue Substances 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 230000008569 process Effects 0.000 description 13
- NOIIUHRQUVNIDD-UHFFFAOYSA-N 3-[[oxo(pyridin-4-yl)methyl]hydrazo]-N-(phenylmethyl)propanamide Chemical compound C=1C=CC=CC=1CNC(=O)CCNNC(=O)C1=CC=NC=C1 NOIIUHRQUVNIDD-UHFFFAOYSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 229920000936 Agarose Polymers 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 239000004831 Hot glue Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000980 acid dye Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to an ethidium bromide adsorption equipment, its aim at provide the device that can adsorb the ethidium bromide of nucleic acid gel electrophoresis in -process, reduce or when stopping EB and pollute the laboratory air, and can improve agarose gel's utilization ratio, and assurance test's homogeneity is favorable to promoting biology and medical science, and the development in fields such as drug design provides safeguard for the the experimenter safe operation, adsorption equipment includes the condensation box and adsorbs the box, under shed on the condensation box, adsorb box bottom portion opening, the top is equipped with the top cap, adsorb box bottom portion with the switch -on of condensation box top, the condensation box includes the condensation layer, condensation layer one side is equipped with the inlet opening, and the opposite side is equipped with the apopore, be equipped with the air vent that runs through top and bottom on the condensation layer, adsorb the box including the adsorbed layer, place the active carbon of ventilated membrane parcel in the adsorbed layer, the adsorbed layer be equipped with with the communicating thru hole of condensation layer air vent.
Description
Technical field
The utility model relates to adsorbent equipment, particularly relates to a kind of ethidium bromide adsorbent equipment, for the absorption of ethidium bromide in Ago-Gel preparation and nucleic acid gel electrophoresis process.
Background technology
The gel electrophoresis of DNA and RNA is one of normal experiment at biology and field of medicaments always, it is no matter the checking at plasmid extraction, the displaying of PCR result, or gene order-checking early stage sample preliminary work recruitment evaluation, nucleic acid gel electrophoresis be all provide for experimenter the information such as nucleic acid purity, concentration, clip size the most directly, most effective method.Ethidium bromide (Ethidiumbromide, the conventional dyes that when being EB) nucleic acid electrophoresis, separate nucleic acid detects, nucleic acid samples is combined with EB and after agarose gel electrophoresis, excite with standard 302nm ultraviolet light transmission instrument and radiate orange red signal, send fluorescence by ultraviolet excitation nucleic acid-EB, observe and the size of definite kernel acid fragment and purity with the gel imaging disposal system shooting of Polaroid egative film or band CCD imaging head.
In nucleic acid gel electrophoresis process, utilize EB dyestuff to have Color is good, highly sensitive, stable in properties, easily store, low price and the advantage such as easy and simple to handle; This technology mainly contains following defect: EB is high causing toxicity material; there is very strong mutagenesis; easily cause cell generation canceration and high volatility (more than 60 ~ 70 DEG C can volatilize) be difficult to again degraded; this requires to use the laboratory of EB to set up special EB contaminated area; carry out nucleic acid electrophoresis simultaneously and carry out careful protection with the experimenter tested that develops, otherwise harm will be produced to the even whole laboratory of experimenter
When carrying out nucleic acid electrophoresis in laboratory, although operating personnel's band gloves operate, avoid EB is infected with human body danger by contact, in electrophoresis process, evaporate aerial EB, there is no at present the measure of test operation personnel protection and preventive means.
Along with the development of biological medicine technology, more and more higher requirement is proposed for the ease for use of DNA and RNA gel electrophoresis and wide usage.In recent years, the possibility studying EB substitute is all being devoted in countries in the world, as with genefinder, syprogreen etc. are nontoxic, low toxicity Fluorescent nucleic acid stain replaces EB, but these dyestuffs significantly limit its application owing to there is the shortcomings such as sensitivity is low, development effect is poor, can not reuse.Therefore, when the gel electrophoresis of DNA and RNA is carried out in current laboratory, most widely used nucleic acid dye remains EB.
Given this, research and develop a kind of device of adsorbable ethidium bromide, to reduce or to purify EB volatilization contaminated air and experimental enviroment, reduce the harm of EB to experimenter and seem very necessary.
Summary of the invention
The technical problems to be solved in the utility model be to provide a kind of can the device of ethidium bromide in adsorbs nucleic acid gel electrophoresis process, reduce or stop EB and pollute while Laboratory air, and the utilization factor of Ago-Gel can be improved, the homogeneity of warranty test, be conducive to promoting biology and medical science, the development in the fields such as drug design, for experimenter's safe operation provides safeguard.
For reaching above-mentioned purpose, a kind of ethidium bromide adsorbent equipment of the utility model, described adsorbent equipment comprises condensation box and adsorption box, described condensation box upper and lower opening, described adsorption box bottom opening, top is provided with top cover, connect with described condensation box top bottom described adsorption box, described condensation box comprises condensate layer, and described condensate layer side is provided with inlet opening, opposite side is provided with apopore, and described condensate layer is provided with the air hole running through top and bottom; Described adsorption box comprises adsorbed layer, and be placed with the activated charcoal of ventilated membrane parcel in described adsorbed layer, described adsorbed layer is provided with the open-work communicated with described condensate layer air hole.
Wherein said condensation box and described adsorption box are aluminum alloy material.
Wherein said condensate layer is also provided with some condensation heads, and described condensation head is conical, and its tip deviates from described adsorption box.
Wherein often row condensation head and often row air hole interval arrange.
Wherein said condensation box and described adsorption box measure-alike.
Wherein said condensation box two bottom sides is provided with electrophoresis tank fixed head, and described electrophoresis tank fixed head is provided with semicircle fixed orifice.
The utility model ethidium bromide adsorbent equipment difference from prior art is that the utility model achieves following technique effect:
The utility model ethidium bromide adsorbent equipment, uses cheap acticarbon, solves because of EB volatilization in glue and electrophoresis process, the problem of test for contamination environment, and in reduction gel electrophoresis assay process, EB is to the harm of experimenter; Meanwhile, gel maker makes gel maker seal because adsorbent equipment covers falling after glue on gel maker, so just avoids glue under open environment to make moisture evaporation, causes gel strength to change, the fidelity of warranty test.
Below in conjunction with accompanying drawing, the utility model is described in further detail.
Accompanying drawing explanation
Fig. 1 is the structural representation of Ago-Gel gel maker;
Fig. 2 is the structural representation of the utility model ethidium bromide adsorbent equipment;
Fig. 3 is the using state figure that the utility model ethidium bromide adsorbent equipment is buckled on gel device;
Fig. 4 is the structural representation of the utility model ethidium bromide adsorbent equipment when being applied to electrophoretic apparatus.
Description of reference numerals: 1-agarose glue module; 2-glue pallet; 3-glue comb; 4-gel maker side seat; 5-condensation box; 6-adsorption box; 7-condensation head; 8-air hole; 9-inlet opening; 10-apopore; 11-electrophoresis tank fixed head; 12-fixed orifice; 13-top cover.
Embodiment
Below in conjunction with drawings and Examples, to above-mentioned being described in more detail with other technical characteristic and advantage of the utility model.
As shown in Figure 2, a kind of ethidium bromide adsorbent equipment of the utility model, described adsorbent equipment comprises condensation box 5 and adsorption box 6, condensation box 5 upper and lower opening, adsorption box 6 bottom opening, top is provided with top cover 13, connect with condensation box 5 top bottom adsorption box 6, condensation box 5 comprises a condensate layer, described condensate layer side is provided with inlet opening 9, opposite side is provided with apopore 10, described condensate layer is provided with the air hole 8 running through top and bottom, adsorption box 6 comprises an adsorbed layer, the activated charcoal of ventilated membrane parcel is placed with in described adsorbed layer, described adsorbed layer is provided with the open-work communicated with described condensate layer air hole.
Embodiment 1: the EB of adsorbing volatilizing in the preparation process of Ago-Gel
As shown in Figure 1, Ago-Gel gel maker is made up of the square agarose glue module 1 of 6 60 × 60mm and gel maker side seat 4.When glue, after melting with the certain density agarose of microwave-oven-heating preparation, add appropriate EB.The Ago-Gel being added with EB is poured in the gel maker installing gel pallet 2 and glue comb 3 in advance, then the condensation box 5 of Fig. 2 ethidium bromide adsorbent equipment is covered on gel maker Fig. 1 (as shown in Figure 3).The adsorbent equipment of ethidium bromide shown in Fig. 2 comprises two box bodys.One is condensation box 5, and comprise a condensate layer, another is adsorption box 6, comprises an adsorbed layer, and condensation box and adsorption box are the box-packed thing of equal-sized rectangular parallelepiped (cooling box 5; Adsorption box 6 is an activated charcoal); The box mouth of condensation box 5 can be buckled on gel maker (Fig. 1) closely.Condensation box 5 and adsorption box 6 are all made with aluminum alloy materials, and adsorbed layer both sides are respectively equipped with inlet opening 9 and apopore 10, before glue, first add 0 DEG C of cold water at the condensate layer of condensation box.Condensate layer is also provided with conical condensation 7 and air hole 8.In glue process, a large amount of water vapor that hot glue volatilizes make water vapor be condensed into water under condensate layer cold service, and most water can be condensed in water droplet instillation gel maker after assembling around taper condensation 7.Air hole 8 on condensate layer runs through condensate layer, arrives adsorbed layer (adsorption box 6), makes the EB volatilized arrive adsorbed layer by air hole 8; Adsorbed layer is adjacent with condensate layer, is provided with the open-work communicated with air hole 8, at the activated charcoal that the adsorbed layer of adsorption box wraps up built with ventilated membrane.The EB volatilized in glue process can be passed through the air hole of condensate layer, arrives adsorbed layer, is absorbed by the activated charcoal in adsorbed layer.Experimentally room environmental temperature height, in glue process, when the water vapor of volatilization is condensed into water at the layer that is not condensed through condensate layer, or condensation effect is deteriorated after glue a couple of days, when activated charcoal becomes wet, arbitrarily can change the activated charcoal in the cold water of condensate layer and adsorbed layer, to ensure the EB that volatilizes being adsorbed as much as possible.
Activated charcoal in the cold water of condensate layer and adsorbed layer can arbitrarily be changed.
Embodiment 2: the EB of adsorbing volatilizing in nucleic acid electrophoresis process
Fig. 4 is the schematic diagram of ethidium bromide adsorbent equipment for nucleic acid electrophoresis process, its mounting structure is identical with Ago-Gel with absorption principle, condensation box 5 lower casing mouth size is identical with electrophoresis tank size, lid mouth plane is arranged fits with electrophoresis tank, and is provided with electrophoresis tank fixed head 11 and the semicircle fixed orifice 12 fixing with electrophoresis tank positive and negative electrode in its laminating plane.After electrophoresis application of sample terminates, ethidium bromide adsorbent equipment (Fig. 4) is buckled on electrophoresis tank, fixing plate hole 12 is buckled on positive and negative electrode.So just can bromizate second ingot adsorbent equipment to be simply fixed on electrophoresis tank, the water vapor condensation that airtight adsorption box just can will volatilize in electrophoresis process, the harmful gases such as the EB of volatilization are by the charcoal absorption of adsorbed layer.Experimentally room environmental temperature height, in electrophoresis process, when the water vapor of volatilization is condensed into water at the layer that is not condensed through condensate layer, or electrophoresis rear condensation effect variation for several times, when activated charcoal becomes wet, arbitrarily can change the activated charcoal in the cold water of condensate layer and adsorbed layer, to ensure the EB that volatilizes being adsorbed as much as possible.
Above-described embodiment is only be described preferred implementation of the present utility model; not scope of the present utility model is limited; under the prerequisite not departing from the utility model design spirit; the various distortion that those of ordinary skill in the art make the technical solution of the utility model and improvement, all should fall in protection domain that the utility model claims determine.
Claims (6)
1. an ethidium bromide adsorbent equipment, it is characterized in that: described adsorbent equipment comprises condensation box and adsorption box, described condensation box upper and lower opening, described adsorption box bottom opening, top is provided with top cover, connects bottom described adsorption box with described condensation box top, described condensation box comprises condensate layer, described condensate layer side is provided with inlet opening, and opposite side is provided with apopore, and described condensate layer is provided with the air hole running through top and bottom; Described adsorption box comprises adsorbed layer, and be placed with the activated charcoal of ventilated membrane parcel in described adsorbed layer, described adsorbed layer is provided with the open-work communicated with described condensate layer air hole.
2. ethidium bromide adsorbent equipment according to claim 1, is characterized in that: described condensation box and described adsorption box are aluminum alloy material.
3. ethidium bromide adsorbent equipment according to claim 1, is characterized in that: described condensate layer is also provided with some condensation heads, and described condensation head is conical, and its tip deviates from described adsorption box.
4. ethidium bromide adsorbent equipment according to claim 3, is characterized in that: often row condensation head and often row air hole interval arrange.
5. ethidium bromide adsorbent equipment according to claim 1, is characterized in that: described condensation box and described adsorption box measure-alike.
6. ethidium bromide adsorbent equipment according to claim 1, is characterized in that: described condensation box two bottom sides is provided with electrophoresis tank fixed head, and described electrophoresis tank fixed head is provided with semicircle fixed orifice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520501975.2U CN204789459U (en) | 2015-07-13 | 2015-07-13 | Ethidium bromide adsorption equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520501975.2U CN204789459U (en) | 2015-07-13 | 2015-07-13 | Ethidium bromide adsorption equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204789459U true CN204789459U (en) | 2015-11-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520501975.2U Expired - Fee Related CN204789459U (en) | 2015-07-13 | 2015-07-13 | Ethidium bromide adsorption equipment |
Country Status (1)
| Country | Link |
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| CN (1) | CN204789459U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106345220A (en) * | 2015-07-13 | 2017-01-25 | 中国农业科学院兰州兽医研究所 | Ethidium bromide adsorbing device |
-
2015
- 2015-07-13 CN CN201520501975.2U patent/CN204789459U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106345220A (en) * | 2015-07-13 | 2017-01-25 | 中国农业科学院兰州兽医研究所 | Ethidium bromide adsorbing device |
| CN106345220B (en) * | 2015-07-13 | 2023-04-28 | 中国农业科学院兰州兽医研究所 | Application of adsorption device in adsorption of volatilized ethidium bromide |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151118 |