CN204779611U

CN204779611U - Automatic incubator of cell

Info

Publication number: CN204779611U
Application number: CN201520323960.1U
Authority: CN
Inventors: 任峰
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-05-19
Filing date: 2015-05-19
Publication date: 2015-11-18
Anticipated expiration: 2025-05-19

Abstract

The utility model relates to an automatic incubator of cell, the incubator includes box, box door, cold -stored device, CO2 child care device, culture medium case or collection box, central incubator, activation case, cell culture bag, micro - camera device and the central control system midway of keeing, cold -stored device, CO2 child care device and the micro - camera device of keeing all is connected respectively with central control system, one side in the box sets up to the cold -stored device of taking care of, and the opposite side in this box sets up to CO2 child care device, and the device is taken care of in this refrigeration and CO2 child care device keeps off looks interval, seal installation through setting up separating in the box. The more traditional manual efficiency of this cell case (appreciation cell quantity) has improved 30%, cell culture automatic control, has realized that cell culture is standardized unitized, has reduced the consumptive material use amount, has reduced the cultivation cost.

Description

The automatic incubator of cell

Technical field

The utility model belongs to medicine instrument technical field, the automatic incubator of especially a kind of cell.

Background technology

Cell cultures be cell under artificial environment, utilize nutritive substance etc., bred increment, and for various object " culturing process.Now scientific research, bio-pharmaceuticals, regenerative medicine, clinical application and genetically engineered etc. are widely used in.

Along with the progress of society, scientific and technological is flourish, and the mankind there has also been higher expectation to quality of life and predicted life.Have a health, happiness, happy life cycle be everyone dream.But as persistent ailments such as tumours, the side effect that traditional treatment means is brought also allows a lot of patient sufferings can't bear.

Clinical cytology treatment is the disease treatment new technology of rising in recent years, refer to the characteristic utilizing some to have the cell of specific function, after employing biological engineering method is obtained and is processed by amplification in vitro, specific culture etc., make these cells have enhancing immunity, kill the therapeutic efficiency such as pathogenic agent and tumour cell, promotion tissue and organ regeneration and physical recovery, thus reach the object of disease therapy.

Cell therapy is with its good curative effect, and side effect is little, and the advantage that more individuation, personalization etc. are unique is the treatment of some refractory diseases, provides a kind of selection, sometimes or even last selection.In the historical stage very long at present and in the future, cell therapy all will take on important role in clinical treatment, and 21st century will be the epoch that cell therapy plays a significant role.

In cell therapy technology, " Cell culture invitro " is the most key, an important ring.It is directly connected to effect and the security for the treatment of.Immunocyte has powerful lethality to cancer cells, and more the immunocyte in patient body is suppressed or damages, quantity also cannot meet clinical demand, and this just needs Applied Biotechnology to cultivate a large amount of highly active immunocyte.Culture is generally individual cells or cell mass.Cell all will be lived in artificial environment when cultivating, and due to the change of environment, the movement of cell or the impact by some other factors, incubation time lengthens.This cell cultures is many carries out in laboratory is by professional and technical personnel's manual operations, but need guarantee the security of institute's culturing cell, never infected.Cultured cell in vitro lacks anti-infection ability, so preventing pollution determines to cultivate successfully or the failed most important condition.Even if in well-appointed laboratory, if operator's technological operation is lack of standardization, also can cause polluting.Therefore every operation process should be guaranteed aseptic, each cultivate link all must accomplish without any confusion, completely reliable.Aseptic technique: sterilization, sterilizing, anticorrosion, antibacterial particularly key.Measure large due to culturing cell kind majority and there is highly active immunocyte, therefore then having strict and high-level requirement to this biotechnology.Current manual operations obviously can not meet the demands such as scientific research, bio-pharmaceuticals, regenerative medicine, clinical application and genetically engineered far away, but also there is a lot of shortcoming.Therefore a kind of new cells produce equipment is needed badly.

To sum up, the shortcoming existed in prior art is as shown in table 1 compared with the utility model:

Table 1 automatic cell culture system and traditional manual operate culture technique difference table

Manual cultivation	Automatic cultivation
		Manual operation, uncertain factor is more, poor repeatability	Totally-enclosed automatic control, repeatability is high
Influence of Evaporation	Substratum horizontal stable
		Man's activity	Computer control, unmanned
Substratum needs people for preheating	Heating, temperature etc. are controlled automatically by system
		In different positions, spatial diversity is larger	Culture bag condition is identical, indifference
Time-consuming and cost is high	Few cost consuming time is low
		Manual sterilization, security risk is large	Automatic disinfection in system, safety

There is ask for something in country to the hospital carrying out immunocyte Vitro Culture Techniques, the Ministry of Health promulgates " medical skill clinical application management method " in 2009, concrete norm provision wherein about application autoimmune cell (T cell, NK cell) treatment technology is pointed out, requirement medical institutions are Grade III Class A hospital, and have the diagnosis and treatment subject relevant with application autoimmune cell (T cell, NK cell) treatment technology of administrative department of public health's approval and register; The laboratory that requirement provides autoimmune cell (T cell, NK cell) preparation to prepare should possess the GMP preparation room of drug regulatory department above the provincial level and disease prevention and control center's certification, has cell collection, processing, calibrating, preservation and clinical application whole process Standard operation procedure SOP (SOP) and complete quality control record; Require that medical personnel must could obtain working qualification through Special Training.

Therefore, cell therapy is carried out for vast clinical hospitals, just there is higher threshold and huge fund input.

By retrieval, not yet find the patent publication us relevant to the utility model patent application.

Summary of the invention

The purpose of this utility model is to overcome the deficiencies in the prior art part, there is provided that one improves working efficiency, cell cultures can control automatically, realize closing cultivation completely, no longer include open link and exist, thoroughly ensured cell biological safely, reduce toxigenic capacity, general environment can be realized use, greatly reduce the automatic incubator of cell of GMP construction and procuring equipment expense.

To achieve these goals, the technical scheme that adopts of the utility model is as follows:

The automatic incubator of a kind of cell, described incubator comprises casing, cabinet door, refrigeration storage appts, CO ₂child care device, substratum case or midway collection box, central incubator, activation box, cell culture bags, micro-photographing apparatus and central control system, described refrigeration storage appts, CO ₂child care device is all connected with central control system respectively with micro-photographing apparatus; Side in described casing is set to refrigerate storage appts, and the opposite side in this casing is set to CO ₂child care device, this refrigeration storage appts and CO ₂child care device by be arranged at barrier in casing separately, sealing installs;

Described refrigeration storage appts comprises reefer system, refrigeration storage appts cavity, substratum case or midway collection box, described reefer system is arranged on the box inside of refrigeration storage appts nearside, described substratum case or midway collection box are arranged in refrigeration storage appts cavity, and this reefer system is used for providing cold storage function to refrigerating storage appts cavity and substratum case or midway collection box; Described CO ₂child care device comprises CO ₂child care device cavity, CO ₂apparatus for controlling of supply, central incubator, activation box and micro-photographing apparatus, described CO ₂apparatus for controlling of supply is arranged at CO ₂on the box inside of child care device nearside, described central incubator, activation box and micro-photographing apparatus are arranged at CO ₂in child care device cavity, described CO ₂apparatus for controlling of supply is used for CO ₂child care device cavity, central incubator and activation box provide and maintain CO ₂within the required range, described central incubator and activation box are juxtaposed on CO to concentration separately ₂the both sides of child care device cavity, described micro-photographing apparatus is located at the top of central incubator and activation box, and this micro-photographing apparatus can measure the quantity of the cell proliferation in central incubator and activation box and be uploaded to central control system;

Described reefer system, CO ₂apparatus for controlling of supply is connected respectively with central control system respectively;

Described cell culture bags be arranged at substratum case or midway collection box, central incubator, on activation box;

Described refrigeration storage appts, CO ₂one side of the casing of child care device side is hinged with cabinet door, and this cabinet door can make after closing to refrigerate storage appts, CO ₂child care device all becomes enclosed space.

And described substratum case or halfway collection box, central incubator, activation box are all from top to bottom respectively at refrigeration storage appts cavity, CO ₂in child care device cavity, parallel interval arranges multiple, micro-photographing apparatus and substratum case or midway collection box, central authorities' incubator, activation box is all equipped with multiple, at each substratum case or midway collection box, central authorities' incubator, the below of activation box is provided with the reeded pallet of top system, the box inside of the two bottom sides of each pallet is provided with the slideway of telescopic, this slideway can slide anteroposterior, this slideway can drive pallet along casing slide anteroposterior, every layer of substratum case or midway collection box and central incubator between be equipped with small-sized heat-sealing machine, this slideway and small-sized heat-sealing machine are connected with central control system respectively and arrange.

And, pallet above the both sides of the groove of the pallet of described central incubator arranges stirring roller, this stirring roller can roll along the longitudinal direction back and forth, stirs roller and is realized by same stepping driven by motor Timing Belt and synchronizing wheel with the stroke of ecto-entad from inside to outside.

And, described cell culture bags comprise substratum or midway reclaim culture bag, central culture bag, activation culture bag and connecting pipeline, described substratum or midway reclaim culture bag, central culture bag is connected with connecting pipeline respectively with activation culture bag; This activation culture bag is used for the activation culture to cell, this central culture bag is for the enlarged culturing to cell, and this substratum or midway are reclaimed culture bag and be used for providing substratum for central culture bag or midway is reclaimed or cultivation terminates rear recovery when central culture bag enlarged culturing; Described substratum or midway are reclaimed culture bag and are placed on substratum case or midway collection box, and described central culture bag is placed on central incubator, and described activation culture bag is placed on activation box.

And, described substratum or midway are reclaimed culture bag and are comprised bag and I liquid-inlet pipe, the edge of sealing is made in described bag four limit heat seal, and at the edge on bag top, coaxial uniform separation seal is fixed with I liquid-inlet pipe, and this I liquid-inlet pipe can be connected with connecting pipeline and be connected;

Described central culture bag comprises bag and II liquid-inlet pipe, and the edge of sealing is made in described bag four limit heat seal, and at the edge on bag top, coaxial separation seal is fixed with II liquid-inlet pipe, and this II liquid-inlet pipe all can be connected connection by connecting pipeline;

Described activation culture bag comprises bag and III liquid-inlet pipe, and the edge of sealing is made in described bag four limit heat seal, and at the edge on bag top, coaxial separation seal is fixed with III liquid-inlet pipe, and this III liquid-inlet pipe all can be connected with connecting pipeline and be connected.

And, described I liquid-inlet pipe, II liquid-inlet pipe and III liquid-inlet pipe are equipped with pipeline switch; Or described I liquid-inlet pipe is round tube type, its quantity is 4; Or described II liquid-inlet pipe is round tube type, its quantity is 3; Or described III liquid-inlet pipe is round tube type, its quantity is 2.

And described I liquid-inlet pipe, II liquid-inlet pipe and III liquid-inlet pipe are all coaxially shaped with the support bar of two symmetries in the end of bag inside; Or, be shaped with ring seal flange collar in the outer end of liquid-inlet pipe, by ring seal flange collar, sealing end cap be coaxially installed; Or the sealing the margin symmetry of the bottom of described bag is shaped with pilot hole; Or, at the connection flank that the coaxial symmetry of liquid-inlet pipe dual-side with bag sealing the margin junction is shaped with.

And described cell culture bags adopts the transparent material meeting plastics Aqueous injection container standard.

And described central control system can control and record all operating process, process, image, data.

Advantage of the present utility model and positively effect as follows:

1, this cell case comparatively traditional-handwork efficiency, increment cell quantity improves 30%, and cell cultures can control automatically, realizes closing completely cultivating, and no longer includes open link and exists, thoroughly ensured cell biological safety; Achieve cell cultures stdn, unitize, the cell cultures of more person-portions can be realized simultaneously, improve efficiency, reduce working strength, decrease consumptive material usage quantity, reduce toxigenic capacity, this incubator can realize general environment and use, and greatly reduces GMP and builds and procuring equipment expense.

2, traditional-handwork cell cultures mode is realized automatization operation system by this cell case, not simple machinery equipment, this system be by various function together with suite, realize the automatically integrating system of cell cultures.Such as, tumour patient immune cell therapy cell rises in value cultivation in a large number, and ACCS both can be used for the cultivation of the immunocyte of person-portion more than ten.The side effect brought due to treatment tumor operation, radiotherapy, embolic chemotherapy is very large, and the general immunity system of tumour patient and immunocyte quantity lowly, cannot be resisted or implement to identify and effectively kill and wound to interior tumor cell.A small amount of (50ml) peripheral blood of venous puncture is (GMP) lymphocyte of being isolated out aseptically, utilizes antibody, biotic factor, substratum etc., carries out activation intervention, amplification, cultivation artificially to cell.Make it rise in value in a large number and possess the ability identifying and kill and wound cancer cells, cultivate after collecting and feed back to again in patient body, implement cancer cells kills behavior, minimum to the side effect of patient.

3, this cell case can be controlled automatically by central control system, improves culture efficiency; Image is shot with video-corder automatically, cell counting, increment curve generate automatically; Culture environment, cultivation stir and automatically control; Cell concentration reclaims, and reduces follow-up cultivation extraction process; Close and cultivate, reduce manual operation, reduce the Pollution risk of context open; Automatic sequence control, by pre-designed best cultivation program, adds supplemental medium automatically; Auto-counting observation, automatic sampling, the automatic recovery, improve stability, the identity of cell quality greatly; Intelligent: can by different cell, the various specific cultivation program of different cultural method setting, solve the loaded down with trivial details and mishandle of artificial culture method; Shorten incubation time, for patient provides cell sooner; Reduce technician's working strength, reduce labour cost expenditure; Reduce consumptive material usage quantity, reduce toxigenic capacity.

4, this cell case can complete filling, the replacing (all or part of) of substratum automatically, and control the height (deviation is at 0.2mm) of substratum, whole flow processs of best cultured cell can be imitated, without the need to manually heating nutrient solution in advance, without the need to manually realizing precisely adding nutrient solution or not timing supplements, in N number of cultivation unit, reach consistent, by computer controlled automatic, whole process can be tested by remote monitoring; High-precision sensor real-time feedback control manages, and provides very stable, highly consistent experiment condition, and has the repeatability of height, without the need to changing culturing process, can turn out high conforming high-quality cell.

5, native system can run automatically without the need to artificially on duty, and resource is more reasonably used; This system can be run weekly 24 hours every days for 7 days, and the restriction of laboratory technician's working hour when not cultivating by artificial cell, eliminates the factor of the main source " people " that cell cultivation process pollutes, ensure that repeatability and the gnotobasis of culturing cell; " frangible " or the clone (as primary cell or stem cell) of complexity in time optimizing clone processing mode and the accurate control to each step---realize automatization cultivation, and there is versatility and adaptability widely.

Accompanying drawing explanation

Fig. 1 is the anatomical connectivity schematic diagram of the utility model incubator;

Fig. 2 is the anatomical connectivity stereographic map that the cabinet door of Fig. 1 is opened;

Fig. 3 is the part sectioned view in Fig. 2;

Fig. 4 is the anatomical connectivity schematic diagram of cell culture bags of one deck pallet in Fig. 1, substratum case or midway collection box, central incubator, activation box;

Fig. 5 is the substratum of cell culture bags in Fig. 4 or the anatomical connectivity front view of midway recovery culture bag;

Fig. 6 is the partial enlarged drawing of the I liquid-inlet pipe in Fig. 5;

Fig. 7 is the axis sectional view of Fig. 6;

Fig. 8 is the right view of Fig. 6;

Fig. 9 is the axis sectional view of Fig. 8.

Embodiment

Below in conjunction with embodiment, further illustrate the utility model, following embodiment is illustrative, is not determinate, can not limit protection domain of the present utility model with following embodiment.

Each syndeton of the present utility model, if no special instructions, can be understood as the ordinary skill in the art.

The automatic incubator of a kind of cell, as shown in Figure 1, Figure 2, Figure 3 and Figure 4, described incubator comprises casing 1, cabinet door 3, refrigeration storage appts (in figure non-label), CO ₂child care device (in figure non-label), substratum case or midway collection box 10, central incubator 12, activation box 15, cell culture bags (in figure non-label), micro-photographing apparatus 9 and central control system 2, described refrigeration storage appts, CO ₂child care device is all connected with central control system respectively with micro-photographing apparatus; Side in described casing is set to refrigerate storage appts, and the opposite side in this casing is set to CO ₂child care device, this refrigeration storage appts and CO ₂child care device by be arranged at barrier (in figure non-label) in casing separately, sealing installs;

Described refrigeration storage appts comprises reefer system 6, refrigeration storage appts cavity 5, substratum case or midway collection box, described reefer system is arranged on the box inside of refrigeration storage appts nearside, described substratum case or midway collection box are arranged in refrigeration storage appts cavity, and this reefer system is used for providing cold storage function to refrigerating storage appts cavity and substratum case or midway collection box; Described CO ₂child care device comprises CO ₂child care device cavity 8, CO ₂apparatus for controlling of supply 7, central incubator, activation box and micro-photographing apparatus, described CO ₂apparatus for controlling of supply is arranged at CO ₂on the box inside of child care device nearside, described central incubator, activation box and micro-photographing apparatus are arranged at CO ₂in child care device cavity, described CO ₂apparatus for controlling of supply is used for CO ₂child care device cavity, central incubator and activation box provide and maintain CO ₂within the required range, described central incubator and activation box are juxtaposed on CO to concentration separately ₂the both sides of child care device cavity, described micro-photographing apparatus is located at the top of central incubator and activation box, and this micro-photographing apparatus can measure the quantity of the cell proliferation in central incubator and activation box and be uploaded to central control system;

In the present embodiment, the storage temperature condition of described reefer system is 4 DEG C, and each independently position is by being placed in the independent temperature device of its internal space to control constant temperature, and realized by refrigeration system and heating system, those structures all can be regarded as prior art;

Described CO ₂child care device is 37 DEG C, CO ₂the concentration 5% of gas, this CO ₂child care device maintains CO ₂5% concentration of gas, Growth of Cells both needed O ₂, need CO again ₂, its suitable atmosphere surrounding is that 95% air adds 5%CO ₂mixed gas, CO ₂required for Growth of Cells, be again products of cellular metabolism simultaneously, CO ₂increasing to make pH value decline.CO ₂environment can be made to maintain PH7.2-7.4, and this is the ecotopia of Growth of Cells.But cell is different, CO ₂different on its impact.

CO ₂the Automatic Control Theory of gas 5% concentration:

CO ₂automatic control CO ₂concentration be pass through CO ₂concentration sensor carries out.CO ₂sensor is used for detecting CO in casing ₂concentration, passes to the control device such as pilot circuit and magnetic valve by detected result, if CO in case detected ₂concentration is on the low side, then magnetic valve is opened, CO ₂enter casing, until CO ₂concentration reaches set concentration, now closed electromagnetic valve, CO in case ₂cut off, reach steady state.CO ₂sampling thief is by CO in case ₂with the thief hatch of the gas sampling after air mixed to machine exterior panel, to use CO at any time ₂concentration determination instrument detects CO ₂concentration whether reach requirement.Current most CO ₂incubator is (its relative humidity level produced can reach about 95%, but after opening the door, humidity resume speed is very slow) that produced moisture by the Evaporation of humidification dish.Because moisture evaporated area is larger, more easily reach the time that the humidity of maximal phase to saturated humidity and after switch gate recovers shorter.So the large culture space of the moisture evaporated area culture space little compared with moisture evaporated area easily keeps maximal phase to saturated humidity.

CO ₂the automatic control structure feature of gas 5% concentration:

1, incubator inner bag adopts mirror face stainless steel or bushing argon arc welding to make, and corner semicircle is easy to clean.

2, the microcomputer temperature controller, temperature fluctuation is little.Case built with adopt the spraying disinfection system of ultrasonic atomization technology and ultraviolet germicidal lamp can timing, regularly carry out disinfection in case, thus to pollute during more effectively preventing cell cultures.

3, the warm warning of independent limit, exceedes limit temperature and namely automatically interrupts, and ensures that experiment safety runs and does not meet accident.

4, door temperature control is adopted effectively can to prevent glass door dew condensation phenomenon in case.

5, water-jacket typ is furnished with microbe filter and is positioned at inlet mouth, provides the gas of 100% filter-sterilized.

6, CO is joined ₂reducing valve (being exclusively used in CO2gas incubator)

7, CO is furnished with ₂tolerance display unit, air storage amount lower than after standard, automatic alarm.CO ₂gas is by the CO outside incubator ₂gas cylinder provides.

Above-mentioned CO ₂apparatus for controlling of supply also can be regarded as prior art.

In the present embodiment, described substratum case or midway collection box, central incubator, activation box are all from top to bottom respectively at refrigeration storage appts cavity, CO ₂in child care device cavity, parallel interval arranges multiple, micro-photographing apparatus and substratum case or midway collection box, central authorities' incubator, activation box is all equipped with multiple, at each substratum case or midway collection box, central authorities' incubator, the below of activation box is provided with the pallet 4 that top is shaped with groove (not shown), the box inside of the two bottom sides of each pallet is provided with the slideway (not shown) of telescopic, this slideway can slide anteroposterior, this slideway can drive pallet along casing slide anteroposterior, every layer of substratum case or midway collection box and central incubator between can be equipped with small-sized heat-sealing machine (not shown), this slideway and small-sized heat-sealing machine are connected with central control system respectively and arrange,

In the present embodiment, pallet above the both sides of the groove of the pallet of described central incubator is arranged and stirs roller 14, this stirring roller can roll along the longitudinal direction back and forth, and stirring roller can be realized by same stepping driven by motor Timing Belt and synchronizing wheel (not shown) with the stroke of ecto-entad from inside to outside.Because cell culture bags can adopt the material meeting plastics Aqueous injection container standard, be flexible materials, so " the rolling " of stirring roller reciprocal both can be stirred the cell in culture bag and substratum fully.Owing to stirring frequently, can be deposited in the old refuse matter (lactic acid etc.) of cell periphery, diffuse to all substratum uniformly, keep the oxygen level of substratum homogeneous, imitated culture environment worsens.

In the present embodiment, described micro-photographing apparatus can adopt the device of patent ZL200820144401.4 " the automatic transmission of test cup and photoelectric double-magnetic circuit detection system ".

In the present embodiment, as shown in Fig. 5, Fig. 6, Fig. 7, Fig. 8 and Fig. 9, described cell culture bags comprise substratum or midway reclaim culture bag 11, central culture bag 13, activation culture bag 16 and connecting pipeline 18, described substratum or midway reclaim culture bag, central culture bag is connected with connecting pipeline respectively with activation culture bag, this activation culture bag is used for the activation culture to cell, this central culture bag is for the enlarged culturing to cell, and this substratum or midway are reclaimed culture bag and be used for providing substratum for central culture bag or midway is reclaimed or cultivation terminates rear recovery when central culture bag enlarged culturing, administration is separated in order to realize plural number, midway is reclaimed cell, the midway of cell is reclaimed and can be carried out 2 times, in central authorities' culture bag, cultured cells is transported in the substratum in left side or the empty bag of midway recovery culture bag, after recovery completes, use the aseptic heat-sealing machine hot pressing in incubator, close and cut off culture bag flexible pipe, take out substratum or midway culture bag, small-sized heat-sealing machine can be equipped with due to every layer of substratum case or between midway collection box and central incubator, the plastic hose (i.e. connecting pipeline) connected between cell culture bags is after pallet playback, just enter in the Heat Sealing groove of heat-sealing machine, when needing the recovery of cultivation midway or cultivation to terminate rear recovery, i.e. hot pressing, close and cut off culture bag flexible pipe, it can under the instruction of central control system freely " ejection " or " playback ", after pallet playback, cultivate cabinet door one and close, the combination of incubator is complete.

Described substratum or midway are reclaimed culture bag and are placed on substratum case or midway collection box, and described central culture bag is placed on central incubator, and described activation culture bag is placed on activation box;

Described substratum or midway are reclaimed culture bag and are comprised bag 22 and I liquid-inlet pipe 21, the edge 24 of sealing is made in described bag four limit heat seal, at the edge on bag top, coaxial uniform separation seal is fixed with I liquid-inlet pipe, in the present embodiment, liquid-inlet pipe is round tube type, its quantity is 4, and this I liquid-inlet pipe all can be connected with connecting pipeline and be connected;

Described central culture bag comprises bag and II liquid-inlet pipe 19, the edge of sealing is made in described bag four limit heat seal, at the edge on bag top, coaxial separation seal is fixed with II liquid-inlet pipe, in the present embodiment, liquid-inlet pipe is round tube type, its quantity is 3, and this II liquid-inlet pipe all can be connected with connecting pipeline and be connected;

Described activation culture bag comprises bag and III liquid-inlet pipe 17, the edge of sealing is made in described bag four limit heat seal, at the edge on bag top, coaxial separation seal is fixed with III liquid-inlet pipe, in the present embodiment, liquid-inlet pipe is round tube type, its quantity is 2, and this III liquid-inlet pipe all can be connected with connecting pipeline and be connected;

The structural similitude of culture bag is reclaimed in the structure of described central culture bag and activation culture bag and substratum or midway, therefore specifically maps;

Described I liquid-inlet pipe, II liquid-inlet pipe and III liquid-inlet pipe are equipped with pipeline switch 20, with easy to use, this I liquid-inlet pipe, II liquid-inlet pipe and III liquid-inlet pipe, can be used for joint detection instrument, exhaust, draw samples and supplemental medium, described I liquid-inlet pipe, II liquid-inlet pipe and III liquid-inlet pipe are all coaxially shaped with the support bar 28 of two symmetries in the end of bag inside, bag effectively can be avoided to be squeezed shutoff liquid-inlet pipe, to ensure that liquid-inlet pipe is unimpeded.

In order to seal liquid-inlet pipe, being shaped with ring seal flange collar 26 in the outer end of liquid-inlet pipe, by ring seal flange collar, sealing end cap 23 being coaxially installed.

Hanging up and using for the ease of culture bag, the sealing the margin symmetry of the bottom of bag is shaped with pilot hole 25.

In order to strengthen the stopping property of liquid-inlet pipe and bag, at the connection flank 27 that the coaxial symmetry of liquid-inlet pipe dual-side with bag sealing the margin junction is shaped with.

Described cell culture bags adopts the transparent material meeting plastics Aqueous injection container standard, and such as this material can be the polyethylene of highly air-permeable.

The advantage of above-mentioned cell culture bags is as follows:

This employing meets the material of plastics Aqueous injection container standard, without stripping material in cell cultivation process, not containing producing dysgenic plasticizer or stripping material to cell; Adopt the polyethylene of highly air-permeable, CO when can meet cell cultures ₂the requirement of the requirement of gas 5% concentration environment and pH value; Adopt needleless connecting joint, so when injecting cell or sampling, do not need to use entry needle, improve the security of operation; Compared with use culturing bottle, gas exchange area is large, is beneficial at limited culture space mass propgation, can obtains the cell of greater amt, closed cultivation, reduces the probability of cell contamination and operator's infection; Material transparent flexible, is convenient under the microscope directly observation of cell state.Liquid inlet and outlet pipe is flexiplast tubing, is convenient to be interconnected close with hot pressing.

In the present embodiment, described central control system can record all operating process, process, image, data, the number of times of the change of temperature in incubation time, culture bag, liquor charging fluid, barcode can be enclosed with the photo etc. of microscope photographing, as with reference to recording and preserving.

The control and management software of above-mentioned central control system can be understood as prior art, also can be as follows:

One, requirement

A. basic demand and the function of developing this software are the reception and the analysis that realize full-automatic cell training system data.

B. the data management based on Access Database Systems makes this software more flexible.

C. the master data flowing of software is the reception of instrument data, the input of patient information, information for hospital, information about doctor, the input of report information, and the data output produced the search request of patient information etc. that associated subscriber proposes.

D. the constrained input treatment scheme of data all relies on the support of database.

E. the safety performance of the safe basic guarantee Access of data is more satisfactory, keeps the compatibility substantially with some other database in this software design.

Two, target

The design objective of this software must reach the saving of manpower and equipment expense as far as possible, and the speed of software data processing is improved, the whole design process of software must by the improve of throughput, and raising of person works's efficiency etc. makes software development cost minimize. realize ensureing that the fund input under the prerequisite of software quality minimizes.

Three, function introduction

1, user logs in

User logs in software by username and password, and each user has different authorities, can realize the function of adding user and amendment authority by user management module.

2, detection module

Can accurately monitoring instrument automatic sample, automatic mixing, automatically test, auto-flushing, and in the database of the software that the test data of instrument is stored.

3, data inquiry module

Data can be detected the same day, also carries out comprehensive inquiry by information such as sample number, test sequence numbers by date inquiries, and with printing function, ink-jet or the laser printer of current main flow can be connected, carry out single report printing or group financial statements print.

4, quality control module

Every day can input detection data, then carrying out standard error analysis by detecting data, forming Quality Control graphic representation.

5, laboratory information custom block

User can define some general information in advance, carries out prompt operation after convenient.

6, user management module

The user of super-ordinate right can be added by this function and delete user, also can revise user right, specifies special user's specific function, management by different levels.

7, help module

Software operating method is easily provided, describes operating process, facilitate user to inquire about.

The advantage of the automatic incubator of the utility model cell is as follows:

(1) save labor force widely, reduce cell cultures cost: the consumption reducing substratum; Reduce artificial expense.

(2) omnidistance controlled, can to operating record.Simple to operate, do not need skilled culture technique.

(3) cultivation performance is improved: measure according to automated cell, can judge that sensitization completes; Multistage liquor charging, stirs at any time, often.

(4) compare manual operations, improve 30 ~ 40% culture efficiency.

(5) security: the supporting tightness system of casing can guarantee sterility; Misoperation is stopped in the management of barcode.

The step of the utility model cell culture operations can be as follows:

1) culture medium bag, cell culture bags, cell activation bag are put into successively the specified location of pallet, connect the pipeline between them.

2), after pallet playback, chamber door is closed, and the combination of incubator is complete.

3) touch control system button: " cultivate and start " → to activation and enlarged culturing transfer.

4) operate the microimaging probe hidden in incubator, observe the change in activation box.

5) constantly monitor active state, captured picture is compared, and can the value-added beginning of automatic decision → shift to enlarged culturing engineering.

6) suitable liquor charging opportunity is preset: can divide for several times to the substratum liquor charging in cell culture bags, due to multistage liquor charging, can ensure that the substratum in culture bag is fresh.

7) cell in culturing process can be observed at any time and measure cell quantity, and keeping survey record result.

8) in time often stir → operation stirs the stroke of roller " from inside to outside and ecto-entad ".

9) cell culture period is about 2 time-of-weeks, cultivates at most the cell of 2L.

The utility model incubator can control envrionment temperature in culture space and humidity, and the utility model temperature and humidity is easy to adjust, simple, economical and practical.The temperature and humidity environment that the utility model provides optimum to control for obtaining the perfect cell effect of propagation.Under the combination of different temperature and humidities, optimum can be obtained and cultivate region.The discrete setting of circuit, gas circuit, fluid path, respectively becomes unit, avoids mutual interference; By computer control air pump, realize pipeline and automatically complete the adding of nutrient solution, rising limit restriction, automatic stirring, automatically clean, determination and analysis unattended operation.Intelligence liquid automatic filling device: can carry out liquid filling to multiple culture bag, filling rate is made by oneself, also can have the function of the some culture bag of filling separately simultaneously as required.

Claims

1. the automatic incubator of cell, is characterized in that: described incubator comprises casing, cabinet door, refrigeration storage appts, CO ₂child care device, substratum case or midway collection box, central incubator, activation box, cell culture bags, micro-photographing apparatus and central control system, described refrigeration storage appts, CO ₂child care device is all connected with central control system respectively with micro-photographing apparatus; Side in described casing is set to refrigerate storage appts, and the opposite side in this casing is set to CO ₂child care device, this refrigeration storage appts and CO ₂child care device by be arranged at barrier in casing separately, sealing installs;

2. the automatic incubator of cell according to claim 1, is characterized in that: described substratum case or halfway collection box, central incubator, activation box are all from top to bottom respectively at refrigeration storage appts cavity, CO ₂in child care device cavity, parallel interval arranges multiple, micro-photographing apparatus and substratum case or midway collection box, central authorities' incubator, activation box is all equipped with multiple, at each substratum case or midway collection box, central authorities' incubator, the below of activation box is provided with the reeded pallet of top system, the box inside of the two bottom sides of each pallet is provided with the slideway of telescopic, this slideway can slide anteroposterior, this slideway can drive pallet along casing slide anteroposterior, every layer of substratum case or midway collection box and central incubator between be equipped with small-sized heat-sealing machine, this slideway and small-sized heat-sealing machine are connected with central control system respectively and arrange.

3. the automatic incubator of cell according to claim 2, it is characterized in that: the pallet above the both sides of the groove of the pallet of described central incubator arranges stirring roller, this stirring roller can roll along the longitudinal direction back and forth, stirs roller and is realized by same stepping driven by motor Timing Belt and synchronizing wheel with the stroke of ecto-entad from inside to outside.

4. the automatic incubator of the cell according to any one of claims 1 to 3, it is characterized in that: described cell culture bags comprise substratum or midway reclaim culture bag, central culture bag, activation culture bag and connecting pipeline, described substratum or midway reclaim culture bag, central culture bag is connected with connecting pipeline respectively with activation culture bag; This activation culture bag is used for the activation culture to cell, this central culture bag is for the enlarged culturing to cell, and this substratum or midway are reclaimed culture bag and be used for providing substratum for central culture bag or midway is reclaimed or cultivation terminates rear recovery when central culture bag enlarged culturing; Described substratum or midway are reclaimed culture bag and are placed on substratum case or midway collection box, and described central culture bag is placed on central incubator, and described activation culture bag is placed on activation box.

5. the automatic incubator of cell according to claim 4, it is characterized in that: described substratum or midway are reclaimed culture bag and comprised bag and I liquid-inlet pipe, the edge of sealing is made in described bag four limit heat seal, at the edge on bag top, coaxial uniform separation seal is fixed with I liquid-inlet pipe, and this I liquid-inlet pipe can be connected with connecting pipeline and be connected;

6. the automatic incubator of cell according to claim 5, is characterized in that: described I liquid-inlet pipe, II liquid-inlet pipe and III liquid-inlet pipe are equipped with pipeline switch; Or described I liquid-inlet pipe is round tube type, its quantity is 4; Or described II liquid-inlet pipe is round tube type, its quantity is 3; Or described III liquid-inlet pipe is round tube type, its quantity is 2.

7. the automatic incubator of cell according to claim 5, is characterized in that: described I liquid-inlet pipe, II liquid-inlet pipe and III liquid-inlet pipe are all coaxially shaped with the support bar of two symmetries in the end of bag inside; Or, be shaped with ring seal flange collar in the outer end of liquid-inlet pipe, by ring seal flange collar, sealing end cap be coaxially installed; Or the sealing the margin symmetry of the bottom of described bag is shaped with pilot hole; Or, at the connection flank that the coaxial symmetry of liquid-inlet pipe dual-side with bag sealing the margin junction is shaped with.

8. the automatic incubator of cell according to claim 4, is characterized in that: described cell culture bags adopts the transparent material meeting plastics Aqueous injection container standard.