CN204752723U - Device is vaccinated with bacterial to fermentation - Google Patents
Device is vaccinated with bacterial to fermentation Download PDFInfo
- Publication number
- CN204752723U CN204752723U CN201520523127.1U CN201520523127U CN204752723U CN 204752723 U CN204752723 U CN 204752723U CN 201520523127 U CN201520523127 U CN 201520523127U CN 204752723 U CN204752723 U CN 204752723U
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- China
- Prior art keywords
- step shaft
- inoculation
- cap
- sanitation
- diaphragm valve
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Abstract
The utility model relates to a device is vaccinated with bacterial to fermentation, it comprises bacterial bottle part, switching subtotal fermentation cylinder part, bacterial bottle part is including bacterial bottle, health level diaphragm valve one, fixed joint, the switching part is including inoculation cap, cap core, the fermentation cylinder part is including fermentation cylinder, inoculation coupling, health level diaphragm valve no. 2. The utility model discloses the disinfection is convenient, and the leakproofness is good, and the inoculation convenience just is difficult for the microbiological contamination.
Description
Technical field
The utility model relates to a kind of inoculator, particularly relates to a kind of fermentation bacterial classification inoculation apparatus.
Background technology
In bio-pharmaceuticals fermentative production, generally all need to carry out the cultivation preparation of strain liquid, and to access bacterial classification in producing be indispensable and very crucial link.Require in biological fermentation process to realize aseptic technique, meet pure-blood ferment.Now widely used classification inoculation apparatus, one adopts to topple over strain liquid inoculation after fermentor tank pressure release, the very easily microbiological contamination of this method; The another kind of method adopting needle injection, this method when syringe needle meticulous, strain liquid not easily enters fermentor tank, and when syringe needle is excessively thick, then the rubber mouth of punctures is too greatly, easy microbiological contamination.The problems such as existing inoculator ubiquity structure is too simple, and stopping property is poor, and sterilization and disinfection is inconvenient.
Utility model content
For the deficiencies in the prior art, it is convenient that the utility model aims to provide a kind of sterilization, good airproof performance, inoculates conveniently and the fermentation bacterial classification inoculation apparatus of not easily microbiological contamination.
Of the present utility modelly to be achieved through the following technical solutions.
A kind of fermentation bacterial classification inoculation apparatus, comprises seed bottle part, changeover section and fermentor tank part, described seed bottle part comprises seed bottle, sanitation-grade diaphragm valve one, permanent joint, one end of described sanitation-grade diaphragm valve one is connected with described seed bottle by thimble connector one, the other end of described sanitation-grade diaphragm valve one is connected with described permanent joint by thimble connector two, and described permanent joint is provided with internal thread, described changeover section comprises inoculation cap, cap core, described cap core comprises the first step shaft, second step shaft, 3rd step shaft, described first step shaft is provided with the outside screw fixing with the screw-internal thread fit of described permanent joint, described first step shaft is axially arranged with hole one, bottom, described hole one is in described second step shaft, described inoculation cap is provided with the through hole coordinated with the first step shaft, described first step shaft is fixed on described inoculation cap through described through hole by gasket one and nut, described second step shaft is laterally provided with hole two, described in described Kong Eryu, hole one is communicated with, described second step shaft external diameter is greater than described first step shaft external diameter and is less than described inoculation cap internal diameter, make to leave gap vertically between described cap core and described inoculation cap, internal thread is provided with in described inoculation cap, described fermentor tank part comprises fermentor tank, inoculated tube joint, sanitation-grade diaphragm valve two, one end of described sanitation-grade diaphragm valve two is connected with described inoculated tube joint by flange one, the other end of described sanitation-grade diaphragm valve two is connected with described fermentor tank by flange two, described inoculated tube joint is provided with the outside screw with the screw-internal thread fit of described inoculation cap, the internal diameter of described inoculated tube joint is greater than the external diameter of described 3rd step shaft, and described cap core is fixed on described inoculated tube joint by gasket two and described inoculation cap.
Above-mentioned fermentation bacterial classification inoculation apparatus, wherein: described sanitation-grade diaphragm valve one is provided with fire resisting shield with the coupling end of described permanent joint.
Above-mentioned fermentation bacterial classification inoculation apparatus, wherein: described 3rd step shaft is provided with scrobicular ring, and described gasket two is stuck on scrobicular ring.
Above-mentioned fermentation bacterial classification inoculation apparatus, wherein: outside described inoculation cap, be provided with rotation long handle.
Above-mentioned fermentation bacterial classification inoculation apparatus, wherein: also comprise sealing nut one, described sealing nut one is provided with the internal thread coordinated with the outside screw of described first step shaft.
Above-mentioned fermentation bacterial classification inoculation apparatus, wherein: also comprise sealing nut two described in sealing nut two and be provided with the internal thread coordinated with the outside screw of inoculated tube joint.
Compared with prior art, the utility model has the advantages that: the utility model seed bottle part can carry out the bottling of bacterial classification at aseptic operating platform, bacterial classification can not be made to infect other bacteriums; The utility model changeover section can be contained in fermentor tank part after high-temperature sterilization; when needing inoculation; under flame protection, seed bottle part is contained on cap core; bacterial classification flows into fermentor tank by inside, not with extraneous contact, is not easy microbiological contamination; strain liquid uninterrupted can be controlled by rotating long handle; obturaged by cap core sealing nut under flame protection after inoculation, can also disassemble at any time and carry out disinfection, after sterilization, refitting is gone back or carries out the inoculation of other fermentor tanks.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the sectional view of the utility model cap core;
Fig. 3 is the partial enlarged view of the utility model when not inoculating;
Partial enlarged view when Fig. 4 is the utility model inoculation;
In figure: A. seed bottle part, B. changeover section, C. fermentor tank part, 1. seed bottle, 2. thimble connector one, 3. sanitation-grade diaphragm valve one, 4. thimble connector two, 5. fire resisting shield, 6. permanent joint, 7. cap core, 8. nut, 9. spring pad, 10. inoculate cap, 11. rotate long handle, 12. gaskets one, 13. gaskets two, 14. inoculated tube joints, 15. flange one, 16. sanitation-grade diaphragm valves two, 17. flanges two, 701. hole two, holes one, 702., 703. first step shafts, 704. second step shafts, 705. the 3rd step shafts.
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described in further detail, but not as restriction of the present utility model.
As shown in Fig. 1 to figure two, a kind of fermentation bacterial classification inoculation apparatus, comprises seed bottle part A, changeover section B and changeover section C, described seed bottle part A comprises seed bottle 1, sanitation-grade diaphragm valve 1, permanent joint 6, one end of described sanitation-grade diaphragm valve 1 is connected with described seed bottle 1 by thimble connector 1, the other end of described sanitation-grade diaphragm valve 1 is connected with described permanent joint 6 by thimble connector 24, and described permanent joint 6 is provided with internal thread, described changeover section B comprises inoculation cap 10, cap core 7, described cap core 7 comprises the first step shaft 703, second step shaft 704, 3rd step shaft 705, described first step shaft 703 is provided with the outside screw fixing with the screw-internal thread fit of described permanent joint 6, described first step shaft 703 is axially arranged with hole 1, bottom, described hole 1 is in described second step shaft 704, described inoculation cap 10 is provided with the through hole coordinated with the first step shaft 703, described first step shaft 703 passes through gasket 1 through described through hole, nut 8 and spring pad 9 are fixed on described inoculation cap 10, described second step shaft 704 is laterally provided with hole 2 702, described hole 2 702 is communicated with described hole 1, described second step shaft 704 external diameter is greater than described first step shaft 703 external diameter and is less than described inoculation cap 10 internal diameter, make to leave gap vertically between described cap core 7 and described inoculation cap 10, internal thread is provided with in described inoculation cap 10, described changeover section C comprises fermentor tank, inoculated tube joint 14, sanitation-grade diaphragm valve 2 16, one end of described sanitation-grade diaphragm valve 2 16 is connected with described inoculated tube joint 14 by flange 1, the other end of described sanitation-grade diaphragm valve 2 16 is connected with described fermentor tank by flange 2 17, described inoculated tube joint 14 is provided with the outside screw with the screw-internal thread fit of described inoculation cap 10, the internal diameter of described inoculated tube joint 14 is greater than the external diameter of described 3rd step shaft 705, and described cap core 7 is fixed on described inoculated tube joint 14 by gasket 2 13 and described inoculation cap 10.
Above-mentioned fermentation bacterial classification inoculation apparatus, wherein: described sanitation-grade diaphragm valve 1 is provided with fire resisting shield 5 with the coupling end of described permanent joint 6.
Above-mentioned fermentation bacterial classification inoculation apparatus, wherein: described 3rd step shaft 705 is provided with scrobicular ring, and described gasket 2 13 is stuck on scrobicular ring.
Above-mentioned fermentation bacterial classification inoculation apparatus, wherein: be provided with outside described inoculation cap 10 and rotate long handle 11.
Above-mentioned fermentation bacterial classification inoculation apparatus, wherein: also comprise sealing nut one (not shown), described sealing nut one (not shown) is provided with the internal thread coordinated with the outside screw of described first step shaft 703.
Above-mentioned fermentation bacterial classification inoculation apparatus, wherein: also comprise sealing nut two (not shown) described in sealing nut two (not shown) and be provided with the internal thread coordinated with the outside screw of inoculated tube joint 14.
Fig. 3 is the utility model when not inoculating, the relative position of inoculation cap 10, cap core 7 and inoculated tube joint 14.
When Fig. 4 is the utility model inoculation, the relative position of inoculation cap 10, cap core 7 and inoculated tube joint 14 and strain liquid flow direction.
During inoculation, realized by following steps:
One, seed bottle part A, changeover section B is taken apart disinfection;
Two, at aseptic working platform, bacterial classification is loaded in seed bottle 1, at aseptic working platform, changeover section B is assembled;
Three, flame high-temperature sterilization is carried out to inoculation tube stub 14, under flame protection, the changeover section B assembled and inoculated tube joint 14 are connected, open sanitation-grade diaphragm valve 2 16, inspection be seal whether intact, if intact continuation next step, if not intact, close sanitation-grade diaphragm valve 2 16 and overhaul;
Four, under the protection of flame, the cap core 7 of seed bottle part A and changeover section B is connected;
Five, opening sanitation-grade diaphragm valve 1, then open and rotate long handle 11 to appropriate position, rotating long handle 11 as being found bad can the closedown at any time of sealing;
Six, under the protection of flame, seed bottle part A is pulled down after inoculation; sealing nut one (not shown) is contained on cap core 7 and tightens; if then during inoculation next time, bacterial classification of the same race can save the sterilization to jointing, or only carries out simple flame disinfection.
When jointing disinfection pulled down by needs, under the protection of flame, jointing can be pulled down, then under the protection of flame, sealing nut two (not shown) is contained on inoculated tube joint 14 and tighten.
The foregoing is only the utility model preferred embodiment; not thereby embodiment of the present utility model and protection domain is limited; to those skilled in the art; should recognize and all should be included in the scheme that equivalent replacement done by all utilization the utility model specification sheetss and diagramatic content and apparent change obtain in protection domain of the present utility model.
Claims (6)
1. a fermentation bacterial classification inoculation apparatus, is characterized in that: comprise seed bottle part, changeover section and fermentor tank part, described seed bottle part comprises seed bottle, sanitation-grade diaphragm valve one, permanent joint, one end of described sanitation-grade diaphragm valve one is connected with described seed bottle by thimble connector one, the other end of described sanitation-grade diaphragm valve one is connected with described permanent joint by thimble connector two, and described permanent joint is provided with internal thread, described changeover section comprises inoculation cap, cap core, described cap core comprises the first step shaft, second step shaft, 3rd step shaft, described first step shaft is provided with the outside screw fixing with the screw-internal thread fit of described permanent joint, described first step shaft is axially arranged with hole one, bottom, described hole one is in described second step shaft, described inoculation cap is provided with the through hole coordinated with the first step shaft, described first step shaft is fixed on described inoculation cap through described through hole by gasket one and nut, described second step shaft is laterally provided with hole two, described in described Kong Eryu, hole one is communicated with, described second step shaft external diameter is greater than described first step shaft external diameter and is less than described inoculation cap internal diameter, make to leave gap vertically between described cap core and described inoculation cap, internal thread is provided with in described inoculation cap, described fermentor tank part comprises fermentor tank, inoculated tube joint, sanitation-grade diaphragm valve two, one end of described sanitation-grade diaphragm valve two is connected with described inoculated tube joint by flange one, the other end of described sanitation-grade diaphragm valve two is connected with described fermentor tank by flange two, described inoculated tube joint is provided with the outside screw with the screw-internal thread fit of described inoculation cap, the internal diameter of described inoculated tube joint is greater than the external diameter of described 3rd step shaft, and described cap core is fixed on described inoculated tube joint by gasket two and described inoculation cap.
2. fermentation bacterial classification inoculation apparatus according to claim 1, is characterized in that: described sanitation-grade diaphragm valve one is provided with fire resisting shield with the coupling end of described permanent joint.
3. fermentation bacterial classification inoculation apparatus according to claim 1, is characterized in that: described 3rd step shaft is provided with scrobicular ring, and described gasket two is stuck on scrobicular ring.
4. fermentation bacterial classification inoculation apparatus according to claim 1, is characterized in that: be provided with rotation long handle outside described inoculation cap.
5. fermentation bacterial classification inoculation apparatus according to claim 1, is characterized in that: also comprise sealing nut one, and described sealing nut one is provided with the internal thread coordinated with the outside screw of described first step shaft.
6. fermentation bacterial classification inoculation apparatus according to claim 1, is characterized in that: also comprise sealing nut two described in sealing nut two and be provided with the internal thread coordinated with the outside screw of inoculated tube joint.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520523127.1U CN204752723U (en) | 2015-07-19 | 2015-07-19 | Device is vaccinated with bacterial to fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520523127.1U CN204752723U (en) | 2015-07-19 | 2015-07-19 | Device is vaccinated with bacterial to fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204752723U true CN204752723U (en) | 2015-11-11 |
Family
ID=54467452
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520523127.1U Expired - Fee Related CN204752723U (en) | 2015-07-19 | 2015-07-19 | Device is vaccinated with bacterial to fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204752723U (en) |
-
2015
- 2015-07-19 CN CN201520523127.1U patent/CN204752723U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151111 Termination date: 20200719 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |