CN204608030U - Culture dish at the bottom of laser scanning co-focusing microscope special efficient glass - Google Patents
Culture dish at the bottom of laser scanning co-focusing microscope special efficient glass Download PDFInfo
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- CN204608030U CN204608030U CN201520260028.9U CN201520260028U CN204608030U CN 204608030 U CN204608030 U CN 204608030U CN 201520260028 U CN201520260028 U CN 201520260028U CN 204608030 U CN204608030 U CN 204608030U
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- culture dish
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- glass
- laser scanning
- polystyrene
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Abstract
The utility model discloses culture dish at the bottom of laser scanning co-focusing microscope special efficient glass, main body is made up of polystyrene, comprise culture dish body (1) and upper cover (2), described culture dish body (1) bottom is provided with manhole, described culture dish is positioned at bottom fixing glass egative film (3) of described through hole, the glass negative (3) of described culture dish bottom centre is Observable district, the polystyrene district of periphery, described Observable district is unobservable district, and surface, described unobservable district is provided with super-hydrophobic nano floor (4).Culture dish at the bottom of the laser scanning co-focusing microscope special efficient glass that the utility model provides, cell can be made only to grow on the glass negative of culture dish bottom centre, and can not in the polystyrene district adherent growth of foster ware bottom periphery, the consumption of nutrient solution can be reduced, reduce and change liquid number of times, reduce experimental cost, also improve the accuracy of cell experiment, have a good application prospect.
Description
Technical field
The utility model relates to cell cultivation equipment technical field in biomedicine experiment, particularly culture dish at the bottom of a kind of laser scanning co-focusing microscope special efficient glass.
Background technology
Cell is basic structure and the functional unit of all life activity, frequently by cell cultures in biomedical research, then microscope is adopted to observe to cell the research carrying out life science, and laser scanning co-focusing microscope is cytogenetic analysis instrument more advanced at present, it installs laser scanning device additional on fluorescent microscope imaging basis, computer is utilized to carry out image procossing, cell, tissue slice that not only observable is fixing, also can observe real-time dynamicly the structure of viable cell, molecule, ion and detect.
When observing viable cell, need with the use of culture dish at the bottom of the special glass of laser scanning co-focusing microscope.Generally be provided with manhole in the bottom of culture dish, and the bottom fixing glass egative film of lead to the hole site is positioned at culture dish, the inside of through hole and glass negative form culture chamber, at the bottom of the special glass of laser scanning co-focusing microscope, culture dish bottom surface is thin, glass base thickness 0.085-0.19mm, light transmission is good, is mainly used in requiring that magnification is high, culture vessel bottom surface transparence requires high microscope experiment, is one of important auxiliary products of laser scanning co-focusing microscope.Be limited to its constructional feature, be only positioned at bottom this culture chamber and just can be observed, the cell on culture chamber inwall can not be observed.But the same nutritive substance consumed in nutrient solution, make cell change the shortening of the liquid timed interval, changing liquid number of times increases, and both wasted nutrient solution, and increased labor capacity again, cell contamination probability also increases.When carrying out cell dyeing or mark, at the bottom of traditional special glass of conventional laser scanning confocal microscope owing to there is a lot of cell in non-viewing district in culture dish, can increase the usage quantity of dye liquor or antibody, cause unnecessary waste.
Summary of the invention
The utility model object is to provide culture dish at the bottom of a kind of laser scanning co-focusing microscope special efficient glass, cell is only grown in the observable district of culture dish bottom centre, can reduce the usage quantity of nutrient solution, reduce and change liquid number of times, thus reduce labour intensity.
Based on the problems referred to above, the technical scheme that the utility model provides is:
Culture dish at the bottom of laser scanning co-focusing microscope special efficient glass, main body is made up of polystyrene, comprise culture dish body and upper cover, described culture dish body bottom portion is provided with manhole, described culture dish is positioned at the bottom fixing glass egative film of described through hole, the glass negative of described culture dish bottom centre is Observable district, and the polystyrene district of periphery, described Observable district is unobservable district, and surface, described unobservable district is provided with super-hydrophobic nano floor.
Preferably, described super-hydrophobic nano layer is for having the polystyrene of super hydrophobic surface, poly (l-lactic acid) or polytetrafluoroethylmaterial material.
Preferably, described super-hydrophobic nano layer is array carbon nano tube film or fluoridizes PEDOT film.
Compared with prior art, the utility model has the advantages that:
1. adopt the technical solution of the utility model, on the super-hydrophobic nano floor surface in the unobservable district of the polystyrene of culture dish bottom periphery, there is super-hydrophobicity, cell is not easily in its surface adhesion and breeding, and only there is the glass negative adherent growth on the surface of water-wet behavior, the cell quantity in the unobservable district of the polystyrene of culture dish bottom periphery is declined, the consumption of nutrient solution can be reduced, but also can reduce and change liquid number of times, thus reduction labour intensity, and the usage quantity of cell dye liquor or antibody can be reduced, avoid waste.
2. adopt the technical solution of the utility model, the contaminated probability of cell can be reduced, particularly for the cell needing long period of experiments, can avoid, by the frequent impact changing nutrient solution, improving the accuracy of cell experiment.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme of the utility model embodiment, below the accompanying drawing used required in describing embodiment is briefly described, accompanying drawing in the following describes is only embodiments more of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation of culture dish embodiment at the bottom of a kind of laser scanning co-focusing microscope special efficient of the utility model glass;
Wherein: 1, culture dish body; 2, upper cover; 3, glass negative; 4, super-hydrophobic nano layer.
Embodiment
Below in conjunction with specific embodiment, such scheme is described further.Should be understood that these embodiments are not limited to for illustration of the utility model limit scope of the present utility model.The implementation condition adopted in embodiment can do further adjustment according to the condition of concrete producer, and not marked implementation condition is generally the condition in normal experiment.
See Fig. 1, for the structural representation of the utility model embodiment, culture dish at the bottom of a kind of laser scanning co-focusing microscope special efficient glass is provided, main body is made up of polystyrene, comprise culture dish body 1 and upper cover 2, wherein the bottom of culture dish body 1 is provided with manhole, culture dish is positioned at the bottom fixing glass egative film 3 of through hole, during cell cultures, cell is often in the glass negative of culture dish bottom centre and the polystyrene district adherent growth of glass negative periphery, but the cell being only positioned at adherent growth on glass negative 3 just can be observed, the cell of polystyrene district adherent growth then can not be observed, cell attachment is cultivated the adherent surface of general requirement and is had wetting ability, only grow on the glass negative 3 of culture dish bottom centre to make cell, and can not in the polystyrene district adherent growth of culture dish bottom periphery, the utility model is provided with one deck super-hydrophobic nano floor 4 in the polystyrene district of culture dish bottom periphery.
Preferred super-hydrophobic nano layer 4 is for having the polystyrene of super hydrophobic surface, poly (l-lactic acid) or polytetrafluoroethylmaterial material, super-hydrophobic nano layer 5 for super-hydrophobic array carbon nano tube film or can also fluoridize PEDOT film, the making method of above-mentioned super-hydrophobic nano layer is the method adopted in prior art water, such as at polystyrene, poly (l-lactic acid) or polytetrafluoroethylmaterial material surface etching, there is the microtexture of certain roughness thus build super hydrophobic surface, adopting the method for vapour deposition prepare super-hydrophobic array carbon nano tube film or fluoridize PEDOT film.
Should be appreciated that, the super-hydrophobic nano layer in the utility model is not limited only to above-described embodiment, and other materials can also be adopted to be prepared.
In sum, culture dish of the present utility model can make cell only grow on the glass negative of culture dish bottom centre, and can not in the polystyrene district adherent growth of culture dish bottom periphery, namely cell is controlled in observable district, the consumption of nutrient solution can be reduced like this, reduce and change liquid number of times, avoid frequently changing the impact of liquid on cell experiment.
Above-mentioned example, only for technical conceive of the present utility model and feature are described, its object is to person skilled in the art person can be understood content of the present utility model and implement according to this, can not limit protection domain of the present utility model with this.All equivalent transformations of doing according to the utility model spirit or modification, all should be encompassed within protection domain of the present utility model.
Claims (3)
1. culture dish at the bottom of laser scanning co-focusing microscope special efficient glass, it is characterized in that: main body is made up of polystyrene, comprise culture dish body (1) and upper cover (2), described culture dish body (1) bottom is provided with manhole, described culture dish is positioned at bottom fixing glass egative film (3) of described through hole, the glass negative (3) of described culture dish bottom centre is Observable district, the polystyrene district of periphery, described Observable district is unobservable district, and surface, described unobservable district is provided with super-hydrophobic nano floor (4).
2. culture dish at the bottom of laser scanning co-focusing microscope special efficient glass according to claim 1, is characterized in that: described super-hydrophobic nano layer (4) is for having the polystyrene of super hydrophobic surface, poly (l-lactic acid) or polytetrafluoroethylmaterial material.
3. culture dish at the bottom of laser scanning co-focusing microscope special efficient glass according to claim 1, is characterized in that: described super-hydrophobic nano layer (4) is for array carbon nano tube film or fluoridize PEDOT film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520260028.9U CN204608030U (en) | 2015-04-27 | 2015-04-27 | Culture dish at the bottom of laser scanning co-focusing microscope special efficient glass |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520260028.9U CN204608030U (en) | 2015-04-27 | 2015-04-27 | Culture dish at the bottom of laser scanning co-focusing microscope special efficient glass |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204608030U true CN204608030U (en) | 2015-09-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520260028.9U Expired - Fee Related CN204608030U (en) | 2015-04-27 | 2015-04-27 | Culture dish at the bottom of laser scanning co-focusing microscope special efficient glass |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204608030U (en) |
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2015
- 2015-04-27 CN CN201520260028.9U patent/CN204608030U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150902 Termination date: 20170427 |
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| CF01 | Termination of patent right due to non-payment of annual fee |