CN204560694U - Plant pathogeny line insect surface sterilization centrifuge shield - Google Patents
Plant pathogeny line insect surface sterilization centrifuge shield Download PDFInfo
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- CN204560694U CN204560694U CN201520219564.4U CN201520219564U CN204560694U CN 204560694 U CN204560694 U CN 204560694U CN 201520219564 U CN201520219564 U CN 201520219564U CN 204560694 U CN204560694 U CN 204560694U
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- inner sleeve
- outer sleeve
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- line insect
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Abstract
The utility model discloses a kind of plant pathogeny line insect surface sterilization centrifuge shield, comprise the inner sleeve for holding nematode liquid to be sterilized and the outer sleeve for collecting sterilization and cleaning waste liquid, inner sleeve is socketed on the upper end of outer sleeve, tube wall below inner sleeve cover junction has the liquid outlet for liquid body exudate, the liquid oozed out from liquid outlet enters outer sleeve.Plant pathogeny line insect liquid to be sterilized is placed in the bottom of inner sleeve, the middle and lower part of inner sleeve is socketed in outer sleeve, the sterilization in inner sleeve and cleaning waste liquid is made to ooze out to enter outer sleeve from the liquid outlet of inner sleeve tube wall middle and lower part and collect, after centrifuge cleaning and sterilizing, the bottom of inner sleeve is the pathogenic nematode disinfected.In whole disinfecting process, thimerosal and cleaning fluid automatically ooze out from liquid outlet through centrifugal action and enter outer sleeve, and pathogenic nematode is in the bottom of inner sleeve always, avoid the damage or quantity loss that may cause pathogenic nematode.
Description
Technical field
The utility model relates to a kind of plant pathogeny line insect surface sterilization device, is specifically related to a kind of plant pathogeny line insect surface sterilization centrifuge shield.
Background technology
Pathogenic nematode surface sterilization is the important step during culture of nematodes and nematode-host study mutually.Method conventional is at present: 1) nematode is put into centrifuge tube, after adding thimerosal sterilization certain hour, topples over or removes upper solution with pipettor; 2) add sterile water wash, be deposited to after bottom centrifuge tube until nematode, topple over or remove upper water liquid with pipettor; 3) repeated washing 2-3 time.The shortcoming of the method is: the process repeatedly toppling over or remove upper solution, can injure or lose nematode, inapplicable a small amount of nematode surface sterilization; Also unskilled neophyty operation is not suitable for.
Utility model content
The purpose of this utility model is to provide a kind of centrifuge shield avoiding plant pathogeny line insect damage and quantity to lose, improve sterilization speed.
The utility model discloses a kind of plant pathogeny line insect surface sterilization centrifuge shield, comprise the inner sleeve for holding nematode liquid to be sterilized and the outer sleeve for collecting sterilization and cleaning waste liquid, inner sleeve is socketed on the upper end of outer sleeve, tube wall below inner sleeve cover junction has the liquid outlet for liquid body exudate, the liquid oozed out from liquid outlet enters outer sleeve.
As preferably, the cylindrical tube of described outer sleeve to be bottom surface be outer convex arc surface.
As preferably, the top of described inner sleeve is cylindrical section, bottom is the arc section of evagination, transition linkage section between top and bottom is followed successively by conical section and cylindrical section, the external diameter of Upper cylindrical section at least equals the internal diameter of described outer sleeve, and the external diameter of bottom arc section is less than the internal diameter of described outer sleeve.
As preferably, described inner sleeve is socketed with the upper end of conical section and described outer sleeve.
As preferably, described liquid outlet has two, and position is symmetrical about the axial centre face of inner sleeve, and shape is square, is embedded with the miillpore filter that aperture is less than pathogenic nematode size.
As preferably, the volume of described outer sleeve at least doubles the volume of inner sleeve.
As preferably, the upper end of described inner sleeve is supporting embedded gag.
As preferably, described inner sleeve, outer sleeve and lid are working of plastics.
Plant pathogeny line insect liquid to be sterilized is placed in the bottom of inner sleeve by the utility model, the middle and lower part of inner sleeve is socketed in outer sleeve, the sterilization in inner sleeve and cleaning waste liquid is made to ooze out to enter outer sleeve from the liquid outlet of inner sleeve tube wall middle and lower part and collect, after centrifuge cleaning and sterilizing, the bottom of inner sleeve is the pathogenic nematode disinfected.In whole disinfecting process, thimerosal and cleaning fluid automatically ooze out from liquid outlet through centrifugal action and enter outer sleeve, and pathogenic nematode is in the bottom of inner sleeve always, avoid the defect of damage that prior art may cause pathogenic nematode or quantity loss.
The utility model coordinates centrifuge to use, and automatically automatically collect waste liquid in the sterilization of plant pathogeny line insect and cleaning process, do not have skill requirement to operating personnel, structure is simple, easy to use.The surface sterilization speed of plant pathogeny line insect greatly can also be accelerated while ensureing the damage that pathogenic nematode may cause or quantity loss.
Accompanying drawing explanation
Fig. 1 is the sectional structure schematic diagram of the utility model embodiment.
Embodiment
As shown in Figure 1, present embodiment discloses a kind of plant pathogeny line insect surface sterilization centrifuge shield, comprise the inner sleeve 1 for holding nematode liquid to be sterilized and the outer sleeve 2 for collecting sterilization and cleaning waste liquid, inner sleeve 1 is socketed on the upper end of outer sleeve 2, the tube wall that inner sleeve 1 overlaps below junction has the liquid outlet 11 for liquid body exudate, the liquid oozed out from liquid outlet 11 enters outer sleeve 2 to be collected.
Outer sleeve 2 for bottom surface be the cylindrical tube of outer convex arc surface.
The top of inner sleeve 1 is cylindrical section, bottom is the arc section of evagination, transition linkage section between top and bottom is followed successively by conical section and cylindrical section, the external diameter of Upper cylindrical section at least equals the internal diameter of outer sleeve 2, and the external diameter of bottom arc section is less than the internal diameter of outer sleeve 2.
Inner sleeve 1 is socketed with the upper end of conical section and outer sleeve 2.
Liquid outlet 11 has two, and position is symmetrical about the axial centre face of inner sleeve 1, and shape is square, is embedded with the miillpore filter 12 that aperture is less than pathogenic nematode size.
In order to make collection waste liquid safer in centrifugal sterilization cleaning process, the volume of outer sleeve 2 is made at least to double the volume of inner sleeve 1.
Liquid in centrifugal sterilization cleaning process in inner sleeve spills from its upper end, and supporting in the upper end of inner sleeve have embedded gag 3.
In order to cost-saving, the material of inner sleeve, outer sleeve and lid all adopts reusable working of plastics.
Plant pathogeny line insect liquid to be sterilized is placed in the bottom of inner sleeve by the utility model, the middle and lower part of inner sleeve is socketed in outer sleeve, the sterilization in inner sleeve and cleaning waste liquid is made to ooze out to enter outer sleeve from the liquid outlet of inner sleeve tube wall middle and lower part and collect, after centrifuge cleaning and sterilizing, the bottom of inner sleeve is the pathogenic nematode disinfected.In whole disinfecting process, thimerosal and cleaning fluid automatically ooze out from liquid outlet through centrifugal action and enter outer sleeve, and pathogenic nematode is in the bottom of inner sleeve always, avoid the defect of damage that prior art may cause pathogenic nematode or quantity loss.The utility model coordinates centrifuge to use, and automatically automatically collect waste liquid in the sterilization of plant pathogeny line insect and cleaning process, do not have skill requirement to operating personnel, structure is simple, easy to use.The surface sterilization speed of plant pathogeny line insect greatly can also be accelerated while ensureing the damage that pathogenic nematode may cause or quantity loss.
Concrete sterilization cleaning step is as follows:
1, plant pathogeny line insect liquid to be sterilized is put into the bottom of inner sleeve, add thimerosal in proportion, cover lid after mixing;
2, after having sterilized, upper and lower for inside and outside sleeve pipe noose is put on the fixed mount of centrifuge well, control centrifuge with the low-speed centrifugal of 2000 ~ 3000 revs/min running 2 ~ 3 minutes, most of thimerosal entered in outer sleeve and discards, only staying in inner sleeve below liquid outlet a small amount of containing nematode thimerosal;
3, internally enough sterile waters are added in sleeve pipe, embedded gag on mixing bonnet; Upper and lower for inside and outside sleeve pipe noose is put into well on centrifuge fixed mount, control centrifuge with the low-speed centrifugal of 2000 ~ 3000 revs/min running 2 ~ 3 minutes, most of water entered in outer sleeve and discards, only staying in inner sleeve below liquid outlet a small amount of containing nematode water;
4, repeat step 3 liang to three times, finally stay the plant pathogeny line insect be through surface sterilization bottom inner sleeve.
Claims (9)
1. a plant pathogeny line insect surface sterilization centrifuge shield, it is characterized in that: it comprises the inner sleeve for holding pathogenic nematode liquid to be sterilized and the outer sleeve for collecting sterilization and cleaning waste liquid, inner sleeve is socketed on the upper end of outer sleeve, tube wall below inner sleeve cover junction has the liquid outlet for liquid body exudate, the liquid oozed out from liquid outlet enters outer sleeve.
2. plant pathogeny line insect surface sterilization centrifuge shield as claimed in claim 1, is characterized in that: the cylindrical tube of described outer sleeve to be bottom surface be outer convex arc surface.
3. plant pathogeny line insect surface sterilization centrifuge shield as claimed in claim 1, it is characterized in that: the top of described inner sleeve is cylindrical section, bottom is the arc section of evagination, transition linkage section between top and bottom is followed successively by conical section and cylindrical section, the external diameter of Upper cylindrical section at least equals the internal diameter of described outer sleeve, and the external diameter of bottom arc section is less than the internal diameter of described outer sleeve.
4. plant pathogeny line insect surface sterilization centrifuge shield as claimed in claim 3, is characterized in that: described inner sleeve is socketed with the upper end of conical section and described outer sleeve.
5. plant pathogeny line insect surface sterilization centrifuge shield as claimed in claim 4, it is characterized in that: described liquid outlet has two, position is symmetrical about the axial centre face of inner sleeve, and shape is square, is embedded with the miillpore filter that aperture is less than pathogenic nematode size.
6. plant pathogeny line insect surface sterilization centrifuge shield as claimed in claim 1, is characterized in that: the volume of described outer sleeve at least doubles the volume of inner sleeve.
7. plant pathogeny line insect surface sterilization centrifuge shield as claimed in claim 2, is characterized in that: the upper end of described inner sleeve is supporting lid.
8. plant pathogeny line insect surface sterilization centrifuge shield as claimed in claim 7, is characterized in that: described lid is embedded gag.
9. plant pathogeny line insect surface sterilization centrifuge shield as claimed in claim 7, is characterized in that: described inner sleeve, outer sleeve and lid are working of plastics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520219564.4U CN204560694U (en) | 2015-04-14 | 2015-04-14 | Plant pathogeny line insect surface sterilization centrifuge shield |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520219564.4U CN204560694U (en) | 2015-04-14 | 2015-04-14 | Plant pathogeny line insect surface sterilization centrifuge shield |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204560694U true CN204560694U (en) | 2015-08-19 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520219564.4U Expired - Fee Related CN204560694U (en) | 2015-04-14 | 2015-04-14 | Plant pathogeny line insect surface sterilization centrifuge shield |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204560694U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601482B (en) * | 2018-12-07 | 2021-06-25 | 江苏科技大学 | Method for preparing sterile pine wood nematodes |
-
2015
- 2015-04-14 CN CN201520219564.4U patent/CN204560694U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601482B (en) * | 2018-12-07 | 2021-06-25 | 江苏科技大学 | Method for preparing sterile pine wood nematodes |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150819 Termination date: 20160414 |
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| CF01 | Termination of patent right due to non-payment of annual fee |