CN204550528U - A kind of cell scuffing experimental device - Google Patents
A kind of cell scuffing experimental device Download PDFInfo
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- CN204550528U CN204550528U CN201520177047.5U CN201520177047U CN204550528U CN 204550528 U CN204550528 U CN 204550528U CN 201520177047 U CN201520177047 U CN 201520177047U CN 204550528 U CN204550528 U CN 204550528U
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- culture dish
- pivoting lid
- cell
- lower cover
- experimental device
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Abstract
The utility model discloses a kind of cell scuffing experimental device, comprise culture dish, culture dish is covered with pivoting lid, and described pivoting lid comprises the upper cover body and lower cover that are consolidated, and the diameter of section that the diameter of section of upper cover body is greater than lower cover is formed and falls the pivoting lid of boss-shaped; Lower cover embeds in culture dish, and the sidewall of lower cover and the inner side-wall of culture dish are fitted, and fit in the sidewall top of the shaft shoulder formed between lower cover and upper cover body and culture dish; Pivoting lid is processed with the pin holes of one group of through pivoting lid, is inserted with the cut draw point stretched in culture dish in each pin holes, rotate pivoting lid, cut draw point can carry out scratch test to the cell in culture dish.Cell scuffing experimental device of the present utility model is simple to operate, good stability, it is even that analog physical damages the scratch width marked, and there will not be cut to bend, departs from and the uncertain problem in position, make experimental result accurate, test raw data the most reliably for follow-up work provides.
Description
Technical field
The utility model belongs to medical instruments field, is specifically related to a kind of cell scuffing experimental device.
Background technology
Cell migration refers to the movement that cell produces after receiving migration signal or experiencing the concentration gradient of Cucumber.In moving process, cell constantly repeats and forwards stretches out cynapse, and then tractive rear cell space travels forward.Cytoskeleton and its associated proteins, intercellular substance is the basic substance of this process in addition, also has many kinds of substance can carry out minute adjustment to it in addition.Cell migration is the displacement undertaken by cell space deformation, and the translational speed of therefore cell migration is very slow.But the motion characteristics of this cell migration " step slow power micro-", be but that cell is looked for food, wound healing, embryo's generation, immune response, involved by the physiological phenomenon such as infection and cancer metastasis.Therefore cell migration is a major subjects of current RESEARCH ON CELL-BIOLOGY, and scientists attempts the research by cell migration, in medical use such as prevention cancer metastasis, allo-skin graft etc., obtain larger achievement.Also because of the motion characteristics that cell migration is exclusive, the popular research direction of biology is still now.
Current research cell migration mainly contains two kinds of experimental technique: transwell and tests and scratch experiment, wherein scratch experiment can damage the migration of rear cell by analog mechanical better, and having the features such as simple to operate, experimental period is short, extracellular environment is stable, is the experimental technique that research cell migration often adopts.
Cell scratch experiment is a kind of simple to operate, economical and practical research cell migration or the ill vitro test method of tumor invasion.This side's ratio juris is, when cell grow to be fused into individual layer state time, artificially on the monolayer cell merged manufacture a white space, be called " cut ".The cell of scratching edge can progress into white space makes " cut " heal.In existing cell scratch experiment, first need to draw horizontal line mark with marker at 6 orifice plates or the 12 orifice plate back sides before culture plate inoculating cell, access in the hole of 6 orifice plates or 12 orifice plates after cell dissociation, after cell is paved with at the bottom of plate, cell cut is manufactured perpendicular to orifice plate with rifle head, scratch width will be kept in scoring processes consistent, and rifle head wants vertical, can not tilt.
In existing cell scratch experiment, cell scuffing experimental device is not complete, and locate the same visual field when conveniently experiment microscope is taken pictures, each laboratory technician needs to use marker to draw horizontal line at the orifice plate back side voluntarily, in order to keep scratch width consistent, rifle head needs to compare ruler and keeps vertical always, makes experimentation to seem loaded down with trivial details.
Owing to there is no fixing, the locating mechanism of cut rod, and the operant level of each laboratory technician is different with custom in experimentation, artificial rifle head is difficult to ensure the consistent of scratch width, cause occurring that cut bends, departing from, scoring position is uncertain, easily cause error in experimentation, affect final experimental result, be difficult to carry out the problems such as follow-up study work.
Summary of the invention
For the deficiency that prior art exists, the purpose of this utility model is, provide a kind of new cell scuffing experimental device, another object of the present utility model is, solve scratch width in prior art uneven and bending, depart from, the uncertain problem in position.
In order to solve the problems of the technologies described above, the utility model adopts following technical scheme to be achieved:
A kind of cell scuffing experimental device, comprise culture dish, culture dish is covered with pivoting lid, pivoting lid is processed with the pin holes of one group of through pivoting lid, the cut draw point stretched in culture dish is inserted with in each pin holes, rotate pivoting lid, cut draw point can carry out scratch test to the cell in culture dish.
The utility model also has distinguishes technical characteristic as follows:
Described pivoting lid comprises the upper cover body and lower cover that are consolidated, and the diameter of section that the diameter of section of upper cover body is greater than lower cover is formed and falls the pivoting lid of boss-shaped;
Lower cover embeds in culture dish, and the sidewall of lower cover and the inner side-wall of culture dish are fitted, and fit in the sidewall top of the shaft shoulder formed between lower cover and upper cover body and culture dish.
Magnet is provided with bottom described culture dish.
Described pin holes three angles be distributed on pivoting lid are the radius of 120 ° with on the intersection point of the involute urve centered by the center of circle of pivoting lid.
The thickness of described pivoting lid is not less than 1.5cm.
The utility model compared with prior art, has following technique effect:
Cell scuffing experimental device of the present utility model is simple to operate, good stability, it is even that analog physical damages the scratch width marked, and there will not be cut to bend, departs from and the uncertain problem in position, make experimental result accurate, test raw data the most reliably for follow-up work provides.
Accompanying drawing explanation
Fig. 1 is schematic cross-section of the present utility model.
Fig. 2 is pin holes distribution schematic diagram of the present utility model.
In figure, the implication of each label is: 1-culture dish, 2-pivoting lid, (2-1)-upper cover body, (2-2)-lower cover, 3-pin holes, 4-cut draw point, 5-magnet.
Below in conjunction with accompanying drawing, explanation is further explained in detail to particular content of the present utility model.
Embodiment
Below provide specific embodiment of the utility model, it should be noted that the utility model is not limited to following specific embodiment, all equivalents done on technical scheme basis all fall into protection domain of the present utility model.
Embodiment:
Defer to technique scheme, as depicted in figs. 1 and 2, the present embodiment provides a kind of cell scuffing experimental device, comprise culture dish 1, culture dish 1 is covered with pivoting lid 2, the diameter of section that the diameter of section that described pivoting lid 2 comprises upper cover body 2-1 and lower cover 2-2, the upper cover body 2-1 be consolidated is greater than lower cover 2-2 is formed and falls the pivoting lid 2 of boss-shaped;
Lower cover 2-2 embeds in culture dish 1, and the sidewall of lower cover 2-2 and the inner side-wall of culture dish 1 are fitted, and fit in the sidewall top of the shaft shoulder formed between lower cover 2-2 and upper cover body 2-1 and culture dish 1;
Pivoting lid 2 is processed with the pin holes 3 of one group of through pivoting lid 2, is inserted with the cut draw point 4 stretched in culture dish 1 in each pin holes 3, rotate pivoting lid 2, cut draw point 4 can carry out scratch test to the cell in culture dish 1.
Be provided with magnet 5 bottom culture dish 1, be used for attracting cut draw point 4 to be in plumbness all the time, can not offset in scoring processes, magnet 5 can adopt permanent magnet, also can adopt electro-magnet.
Pin holes 3 three angles be distributed on pivoting lid 2 are the radius of 120 ° with on the intersection point of the involute urve centered by the center of circle of pivoting lid 2.The radius of the circular cut that every root cut draw point 4 is formed in rotary course is all different, is unlikely repetition, also not overlapping, and cut draw point 4 is effectively utilized, and is also convenient to the carrying out of testing.
The thickness of pivoting lid 2 is not less than 1.5cm, makes cut draw point 4 can stable existence in pin holes 3, and do not rock, the thickness of pivoting lid 2 is too little, and cut draw point 4 can rock in rotation process.
Cell scuffing experimental device of the present utility model in use, nutrient solution containing cell is put into cultivation, in 1, covers pivoting lid 2, need to insert corresponding cut draw point 4 according to different experiments, rotate pivoting lid 2, drive cut draw point 4 cut, because pivoting lid 2 and culture dish 1 assemble well, can not produce in rotation process and rock, draw point is under the acting in conjunction of magnet 5 with the pivoting lid 2 having thickness suitable, and also can not rock in pin holes 3, therefore cut quality is high.
Claims (5)
1. a cell scuffing experimental device, comprise culture dish (1), it is characterized in that: culture dish (1) is covered with pivoting lid (2), pivoting lid (2) is processed with the pin holes (3) of one group of through pivoting lid (2), the cut draw point (4) stretched in culture dish (1) is inserted with in each pin holes (3), rotate pivoting lid (2), cut draw point (4) can carry out scratch test to the cell in culture dish (1).
2. cell scuffing experimental device as claimed in claim 1, it is characterized in that: described pivoting lid (2) comprises the upper cover body (2-1) and lower cover (2-2) that are consolidated, the diameter of section that the diameter of section of upper cover body (2-1) is greater than lower cover (2-2) is formed and falls the pivoting lid (2) of boss-shaped;
Lower cover (2-2) embeds in culture dish (1), the sidewall of lower cover (2-2) and the inner side-wall of culture dish (1) are fitted, and fit in the sidewall top of the shaft shoulder formed between lower cover (2-2) and upper cover body (2-1) and culture dish (1).
3. cell scuffing experimental device as claimed in claim 1, is characterized in that: described culture dish (1) bottom is provided with magnet (5).
4. cell scuffing experimental device as claimed in claim 1, is characterized in that: described pin holes (3) three angles be distributed on pivoting lid (2) are the radius of 120 ° with on the intersection point of the involute urve centered by the center of circle of pivoting lid (2).
5. cell scuffing experimental device as claimed in claim 1, is characterized in that: the thickness of described pivoting lid (2) is not less than 1.5cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520177047.5U CN204550528U (en) | 2015-03-26 | 2015-03-26 | A kind of cell scuffing experimental device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520177047.5U CN204550528U (en) | 2015-03-26 | 2015-03-26 | A kind of cell scuffing experimental device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204550528U true CN204550528U (en) | 2015-08-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520177047.5U Expired - Fee Related CN204550528U (en) | 2015-03-26 | 2015-03-26 | A kind of cell scuffing experimental device |
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| Country | Link |
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| CN (1) | CN204550528U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520525A (en) * | 2017-01-01 | 2017-03-22 | 重庆科技学院 | Cell scratch test cover |
| CN106701534A (en) * | 2017-01-01 | 2017-05-24 | 重庆科技学院 | Application method of cell wound scratch assay cover |
-
2015
- 2015-03-26 CN CN201520177047.5U patent/CN204550528U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520525A (en) * | 2017-01-01 | 2017-03-22 | 重庆科技学院 | Cell scratch test cover |
| CN106701534A (en) * | 2017-01-01 | 2017-05-24 | 重庆科技学院 | Application method of cell wound scratch assay cover |
| CN106520525B (en) * | 2017-01-01 | 2019-03-05 | 重庆科技学院 | A kind of cell scratch experiment lid |
| CN106701534B (en) * | 2017-01-01 | 2019-04-05 | 重庆科技学院 | A kind of application method of cell scratch experiment lid |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150812 Termination date: 20160326 |