CN204462150U - A kind of microplate reader - Google Patents

A kind of microplate reader Download PDF

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Publication number
CN204462150U
CN204462150U CN201520203234.6U CN201520203234U CN204462150U CN 204462150 U CN204462150 U CN 204462150U CN 201520203234 U CN201520203234 U CN 201520203234U CN 204462150 U CN204462150 U CN 204462150U
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China
Prior art keywords
microplate reader
key
enzyme mark
display
main body
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Expired - Fee Related
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CN201520203234.6U
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Chinese (zh)
Inventor
白东峰
朱清智
卢宏炎
邓晓
王毅
华显立
贺军峰
许贵阳
倪江楠
连晗
张成光
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Henan Polytechnic Institute
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Henan Polytechnic Institute
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Priority to CN201520203234.6U priority Critical patent/CN204462150U/en
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Abstract

The utility model belongs to medical detection technology, relates to a kind of microplate reader specifically, comprises microplate reader main body, measuring chamber and control display, and microplate reader main body comprises power light, switch, fuse, thermovent, electrostatic prevention power lead, measuring chamber comprises base, slide rail, enzyme mark supporting plate, enzyme mark orifice plate, containing hole, sample Container, shading cover plate, edge light barrier, control display and comprise pedestal, brightness regulator, restart key, status indicator lamp, display screen, display operating key, mode selecting key, numerical key, detect manipulation key, microplate reader of the present utility model is with temperature sensor, the extension line of temperature sensor is connected with temperature polling instrument, realize the accurate control to experimental temperature, the accuracy of Enhancement test, LED is as detection light source, substantially prolongs the serviceable life of detection light source, saved a large amount of electric energy simultaneously, described measuring chamber base adds slideway, use disposable sample Container, avoid cross pollution.

Description

A kind of microplate reader
Technical field
The utility model belongs to medical detection technology, relates to a kind of microplate reader specifically.
Background technology
Microplate reader is the instrumentation of enzyme linked immunosorbent assay, be widely used in clinical examination, biological study, agricultural sciences, in food and environmental science, the use of microplate reader avoids the comparatively big error caused when original range estimation judges, application in reproductive health care field is more and more extensive, facilitate the raising of healthy reproduction technical merit, sample to be measured is placed in enzyme mark orifice plate by microplate reader in use, make light vertically through the sample solution to be measured in enzyme mark orifice plate, carry out Collection and analysis by photoelectricity receiving device to the light signal through sample solution to obtain a result, the enzyme mark orifice plate of existing microplate reader is provided with many micropores and is used for accommodating sample solution, enzyme mark orifice plate needs clean clean after using once, but because enzyme mark orifice plate is provided with micropore, so clean very difficult, and when sample solution itself is containing some pollutants or pathogen, does not wash clean clearly and also can cause cross pollution, in enzyme linked immunosorbent assay, temperature controlled accuracy directly has influence on the accuracy of enzymatic activity and immunoassay result mensuration, and existing microplate reader does not have the function of accurate control temperature, existing microplate reader, most halogen tungsten lamp that uses is as detection light source, and this light source is short for serviceable life, and electrical source consumption energy is large, existing microplate reader, use and cleaning of enzyme mark orifice plate and enzyme mark supporting plate time cumbersome, therefore, how to control the temperature of microplate reader, adopt long-life cold light source replacement halogen tungsten lamp light source, how to make easy to clean become urgent problem.
Summary of the invention
In order to overcome deficiency existing in background technology, the utility model proposes a kind of microplate reader, described microplate reader is provided with temperature sensor, and the extension line of temperature sensor is connected with temperature polling instrument, realize accurately controlling the temperature of experiment, the accuracy of Enhancement test result; Described microplate reader uses LED as detection light source, substantially prolongs the serviceable life of detection light source, has saved a large amount of electric energy simultaneously; Described microplate reader measuring chamber base adds slideway, facilitate enzyme mark supporting plate, the installation of enzyme mark orifice plate, dismounting, cleaning, described microplate reader uses disposable sample Container, avoids because enzyme mark orifice plate does not wash clean clearly the cross pollution caused, can split connecting band as required to choose appropriate specimen cup and detect, testing process is convenient and safe.
To achieve these goals, the utility model is achieved by the following technical solution:
Described microplate reader, comprise microplate reader main body, measuring chamber and control display, described microplate reader main body upper side left part is provided with measuring chamber, microplate reader main body right part is provided with control display, microplate reader main body comprises power light, switch, fuse, thermovent, electrostatic prevention power lead, left end on microplate reader main body leading flank is provided with power light, and the front end on left surface is provided with switch, and the rear end of left surface is provided with fuse, fuse rear end is provided with thermovent, and trailing flank left end is provided with electrostatic prevention power lead, measuring chamber comprises base, slide rail, enzyme mark supporting plate, enzyme mark orifice plate, containing hole, shading cover plate, edge light barrier, described sample Container comprises specimen cup, connecting band, adjacent two specimen cups are linked together by connecting band, described floor installation is on support, the bottom of base is provided with slide rail, described enzyme mark supporting plate is arranged on base by slide rail, enzyme mark supporting plate is provided with fixed placement groove, described enzyme mark orifice plate is arranged on enzyme mark supporting plate by fixed placement groove, before described base, rear two ends are provided with slideway, described shading cover plate is arranged on the upper end of base, described base is provided with edge light barrier at the closing end of shading cover plate, control display and comprise pedestal, brightness regulator, restart key, status indicator lamp, display screen, display operating key, mode selecting key, numerical key, detect manipulation key, pedestal is provided with bottom controller, the left side controlling display upper side is provided with brightness regulator, brightness regulator upper end is provided with restarts key, the upper right side controlled in display upper side is provided with status indicator lamp, control display top and be provided with display screen, display operating key is provided with below display screen, display controller right lower quadrant is provided with mode selecting key, mode selecting key left is provided with numerical key.
Further, described enzyme mark supporting plate is provided with temperature sensor, and this temperature sensor and temperature polling instrument are electrically connected.
Further, described containing hole has N number of, N >=1, and is evenly distributed on enzyme mark orifice plate, uses disposable sample Container, and the distance of adjacent two containing holes is identical with the distance of adjacent two specimen cups, and described connecting band is single row type detachable structure.
Further, measuring chamber base adds slideway.
Further, described detection light source is LED.
The beneficial effects of the utility model:
1. adopt temperature sensor, can realize the accurate control to experimental temperature, temperature controlled accuracy directly has influence on the accuracy of enzymatic activity and immunoassay result mensuration, and therefore, serviceability temperature sensor can increase the accuracy of experiment.
2. on base, add slideway, facilitate the installation of enzyme mark supporting plate and enzyme mark orifice plate, dismounting, cleaning, also uses disposable sample Container, had both solved enzyme mark supporting plate, the problem of enzyme mark orifice plate cleaning difficulty, turn avoid sample solution and remains in phenomenon enzyme mark orifice plate causing cross pollution.
3. use disposable sample Container, adjacent two specimen cups of disposable sample Container are connected by connecting band, and can split connecting band as required and choose appropriate specimen cup and detect, testing process is convenient and safe.
4. use LED cold light source as detection light source, substantially prolongs the serviceable life of detection light source, saved a large amount of electric energy simultaneously, reduce production cost and the use cost of microplate reader.
Accompanying drawing explanation
Accompanying drawing 1 is microplate reader agent structure schematic diagram;
Accompanying drawing 2 is measuring chamber structural representation;
Accompanying drawing 3 is the structural representation of sample Container;
Accompanying drawing 4 is for controlling display schematic diagram;
Accompanying drawing 5 is temperature sensor operating diagram.
In figure: 1-microplate reader main body, 2-measuring chamber, 3-controls display, 4-power light, 5-switch, 6-fuse, 7-thermovent, 8-electrostatic prevention power lead, 9-shading cover plate, 10-base, 11-edge light barrier, 12-slide rail, 13-enzyme mark supporting plate, 14-enzyme mark orifice plate, 15-containing hole, 16-sample Container, the disposable sample Container of 17-, 18-specimen cup, 19-connecting band, 20-mode selecting key, 21-numerical key, 22-detects manipulation key, 23-pedestal, 24-brightness regulator, 25-restarts key, 26-status indicator lamp, 27-display screen, 28-display operating key, 29-temperature polling instrument, 30-temperature sensor.
Embodiment
In order to make the purpose of this utility model, technical scheme and beneficial effect clearly, below in conjunction with accompanying drawing, preferred embodiment of the present utility model is described in detail, to facilitate the technical staff to understand.
The technical solution adopted in the utility model is:
As Figure 1-5, a kind of microplate reader, comprise microplate reader main body 1, measuring chamber 2 and control display 3, microplate reader main body 1 comprises power light 4, switch 5, fuse 6, thermovent 7, electrostatic prevention power lead 8, microplate reader main body 1 upper side left part is provided with measuring chamber 2, microplate reader main body 1 right part is provided with and controls display 3, left end on the leading flank of microplate reader main body 1 is provided with power light 4, the front end of microplate reader main body 1 left surface is provided with switch 5, microplate reader main body 1 trailing flank left end is provided with fuse 6, fuse 6 rear end is provided with thermovent 7, on rear side of microplate reader main body, left end is provided with electrostatic prevention power lead 8.
Measuring chamber comprises base 10, slide rail 12, enzyme mark supporting plate 13, enzyme mark orifice plate 14, containing hole 15, shading cover plate 9, edge light barrier 11, control display 3 and comprise pedestal 23, brightness regulator 24, restart key 25, status indicator lamp 26, display screen 27, display operating key 28, mode selecting key 20, numerical key 21, detect manipulation key 22, microplate reader of the present utility model is with temperature sensor 30, the extension line of temperature sensor 30 is connected with temperature polling instrument 29, realize accurately controlling the temperature of experiment, the accuracy of Enhancement test, LED is as detection light source, substantially prolongs the serviceable life of detection light source, saved a large amount of electric energy simultaneously, measuring chamber base adds slideway simultaneously, also use disposable sample Container, both enzyme mark supporting plate had been solved, the problem of enzyme mark orifice plate cleaning difficulty, turn avoid sample solution and remain in phenomenon enzyme mark orifice plate causing cross pollution.
Control display 3 and comprise pedestal 23, brightness regulator 24, restart key 25, status indicator lamp 26, display screen 27, display operating key 28, mode selecting key 20, numerical key 21, detect manipulation key 22, control to be provided with pedestal 23 bottom display 3, the left side controlling display 3 upper side is provided with brightness regulator 24, brightness regulator 24 upper end is provided with restarts key 25, the upper right side controlled in display 3 upper side is provided with status indicator lamp 26, control display 3 top and be provided with display screen 27, display operating key 28 is provided with below display screen 27, display operating key 28 right lower quadrant is provided with mode selecting key 20, mode selecting key 20 left is provided with numerical key 21.
The course of work of the present utility model: the principle of work of microplate reader is as follows, the light that illuminator sends after filtration mating plate or monochromator becomes a monochromic beam, inject the sample to be measured in containing hole, this light part is by light absorption, another part is then irradiated on photoelectric detector through sample, by this sample difference to be measured, strong and weak different light signal changes into corresponding electric signal to photoelectric detector, electric signal is through enlarge leadingly, logarithmic amplification, send into microprocessor after the signal processings such as analog to digital conversion and carry out data processing and calculating, finally show result by display and printer.
Light is by detected material, and namely the capacity volume variance of front and back is the energy that detected material sponges, and under specific wavelength, the concentration of same detected material becomes quantitative relationship with absorbed energy.Measure each material and have its specific wavelength, at this wavelength, this material can absorb maximum luminous energies.If select other wavelength period, will cause the inaccurate of testing result, therefore, when measuring detection thing, we select specific wavelength to detect, and are called measurement wavelength.But each material also exists certain non-specific adsorption to luminous energy, in order to eliminate this non-specific adsorption, we choose one again with reference to wavelength, to eliminate this inaccuracy.Under reference wavelength, the absorption detecting object light is minimum.The difference of the light absorption value of determined wavelength and reference wavelength just can eliminate non-specific adsorption.
The utility model comprises microplate reader main body 1, measuring chamber 2 and control display 3.Microplate reader main body 1 comprises power light 4, switch 5, fuse 6, thermovent 7, electrostatic prevention power lead 8, microplate reader main body 1 upper side left part is provided with measuring chamber 2, and microplate reader main body 1 right part is provided with and controls display 3, and the left end on the leading flank of microplate reader main body 1 is provided with power light 4, the front end of microplate reader main body 1 left surface is provided with switch 5, microplate reader main body 1 trailing flank left end is provided with fuse 6, and fuse 6 rear end is provided with thermovent 7, and on rear side of microplate reader main body, left end is provided with electrostatic prevention power lead 8.
Measuring chamber 2 comprises base 10, slide rail 12, enzyme mark supporting plate 13, enzyme mark orifice plate 14, containing hole 15, shading cover plate 9, edge light barrier 11, disposable sample Container 17 comprises specimen cup 18 and connecting band 19, slide rail 12 is arranged on base 10, then adjacent two specimen cups 18 are linked together by connecting band 19 and form disposable sample Container 17, both enzyme mark orifice plate 14 had been solved, enzyme mark supporting plate 13 cleans the problem of difficulty, turn avoid sample solution and remain in situation enzyme mark orifice plate 14 causing cross pollution, and adjacent two specimen cups 18 of disposable sample Container 17 are connected by connecting band 19, connecting band 19 can be split as required choose appropriate specimen cup 18 and detect, testing process is convenient and safe, containing hole 15 is offered again on enzyme mark orifice plate 14, disposable sample Container 17 is placed on enzyme mark orifice plate 14 by containing hole 15, then on enzyme mark orifice plate 14, fixed placement groove is offered, enzyme mark orifice plate 14 is arranged on enzyme mark supporting plate 13 by fixed placement groove, again enzyme mark supporting plate 13 is arranged on base 10 by slide rail 12, two sections, the front and back of base 10 arrange slideway 12, slideway 12 facilitates enzyme mark supporting plate 13, the installation of enzyme mark orifice plate 14, dismounting, cleaning.Shading cover plate 9 is arranged on the upper end of base 10 by slideway 12, then on base 10, edge light barrier 11 is set at the closing end of shading cover plate 9.
Control display 3 and comprise pedestal 23, brightness regulator 24, restart key 25, status indicator lamp 26, display screen 27, display operating key 28, mode selecting key 20, numerical key 21, detect manipulation key 22, pedestal 23 is provided with bottom controller 3, the left side controlling display 3 upper side is provided with brightness regulator 24, brightness regulator 24 upper end is provided with restarts key 25, the upper right side controlled in display 3 upper side is provided with status indicator lamp 26, control display 3 top and be provided with display screen 27, display operating key 28 is provided with below display screen 27, display operating key 28 right lower quadrant is provided with mode selecting key 20, mode selecting key 20 left is provided with numerical key 21.
Microplate reader described in the utility model, enzyme mark supporting plate 13 with temperature sensor 30 is placed in microplate reader main body 1, what temperature sensor adopted U.S. DALLAS semiconductor company to release can only temperature sensor DS18B20, temperature measurement range is-55-+125, completely system requirements can be met, close door, the extension line of temperature sensor 30 is connected with temperature polling instrument 29, open microplate reader power supply, set the temperature of microplate reader and wait for the stable of each temperature sensor reading, the temperature control situations of microplate reader main body 1 in each region of enzyme mark supporting plate 13 can be obtained, and then achieve the temperature accurately being controlled this enzyme mark supporting plate 13 regional by the adjustment of microplate reader main body 1 temperature, thus also just can realize accurately controlling the temperature of experiment, enhance the accuracy of experiment.Microplate reader described in the utility model needs to adopt long-life cold light source LED as detection light source, substantially prolongs the serviceable life of detection light source, saved a large amount of electric energy simultaneously, reduce production cost and the use cost of microplate reader, the base 10 of measuring chamber 2 adds slide rail 12, facilitate enzyme mark supporting plate 13, the installation of enzyme mark orifice plate 14, dismounting, cleaning, and adjacent two specimen cups 18 of disposable sample Container 17 are connected by connecting band 19, connecting band 19 can be split as required choose appropriate specimen cup 18 and detect, testing process is convenient and safe.
What finally illustrate is, above preferred embodiment is only in order to illustrate the technical solution of the utility model and unrestricted, although be described in detail the utility model by above preferred embodiment, but those skilled in the art are to be understood that, various change can be made to it in the form and details, and not depart from the utility model claims limited range.

Claims (5)

1. a microplate reader, comprise microplate reader main body, measuring chamber and control display, it is characterized in that: described microplate reader main body upper side left part is provided with measuring chamber, microplate reader main body right part is provided with control display, microplate reader main body comprises power light, switch, fuse, thermovent, electrostatic prevention power lead, left end on microplate reader main body leading flank is provided with power light, and the front end on left surface is provided with switch, and the rear end of left surface is provided with fuse, fuse rear end is provided with thermovent, and trailing flank left end is provided with electrostatic prevention power lead, measuring chamber comprises base, slide rail, enzyme mark supporting plate, enzyme mark orifice plate, containing hole, shading cover plate, edge light barrier, sample Container comprises specimen cup, connecting band, adjacent two specimen cups are linked together by connecting band, described floor installation is on support, the bottom of base is provided with slide rail, described enzyme mark supporting plate is arranged on base by slide rail, enzyme mark supporting plate is provided with fixed placement groove, described enzyme mark orifice plate is arranged on enzyme mark supporting plate by fixed placement groove, the rear and front end of described base is provided with slideway, described shading cover plate is arranged on the upper end of base, described base is provided with edge light barrier at the closing end of shading cover plate, control display and comprise pedestal, brightness regulator, restart key, status indicator lamp, display screen, display operating key, mode selecting key, numerical key, detect manipulation key, pedestal is provided with bottom controller, the left side controlling display upper side is provided with brightness regulator, brightness regulator upper end is provided with restarts key, the upper right side controlled in display upper side is provided with status indicator lamp, control display top and be provided with display screen, display operating key is provided with below display screen, display controller right lower quadrant is provided with mode selecting key, mode selecting key left is provided with numerical key.
2. a kind of microplate reader as claimed in claim 1, is characterized in that: enzyme mark supporting plate is provided with temperature sensor, and this temperature sensor and temperature polling instrument are electrically connected.
3. a kind of microplate reader as claimed in claim 1, it is characterized in that: described containing hole has N number of, N >=1, and be evenly distributed on enzyme mark orifice plate, the disposable sample Container of movable placement on containing hole, the distance of adjacent two containing holes is identical with the distance of adjacent two specimen cups, and described connecting band is single row type detachable structure.
4. a kind of microplate reader as claimed in claim 1, is characterized in that: measuring chamber base adds slideway.
5. a kind of microplate reader as claimed in claim 1, is characterized in that: described detection light source is LED.
CN201520203234.6U 2015-04-07 2015-04-07 A kind of microplate reader Expired - Fee Related CN204462150U (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107121471A (en) * 2017-06-27 2017-09-01 苏州奥特科然医疗科技有限公司 A kind of ELIASA
CN107271516A (en) * 2017-06-27 2017-10-20 苏州奥特科然医疗科技有限公司 A kind of ELIASA
CN107505310A (en) * 2017-08-15 2017-12-22 深圳市易瑞生物技术股份有限公司 Bio-energy shifts luminescence analyzer
WO2018006466A1 (en) * 2016-07-08 2018-01-11 沈阳医学院 Freely-assembled enzyme label plate
CN107991447A (en) * 2017-11-13 2018-05-04 无锡艾科瑞思产品设计与研究有限公司 A kind of trade effluent detection device

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018006466A1 (en) * 2016-07-08 2018-01-11 沈阳医学院 Freely-assembled enzyme label plate
US10507467B2 (en) 2016-07-08 2019-12-17 Shenyang Medical College Freely-assembled ELISA plate
CN107121471A (en) * 2017-06-27 2017-09-01 苏州奥特科然医疗科技有限公司 A kind of ELIASA
CN107271516A (en) * 2017-06-27 2017-10-20 苏州奥特科然医疗科技有限公司 A kind of ELIASA
CN107505310A (en) * 2017-08-15 2017-12-22 深圳市易瑞生物技术股份有限公司 Bio-energy shifts luminescence analyzer
CN107505310B (en) * 2017-08-15 2019-11-15 深圳市易瑞生物技术股份有限公司 Bio-energy shifts luminescence analyzer
CN107991447A (en) * 2017-11-13 2018-05-04 无锡艾科瑞思产品设计与研究有限公司 A kind of trade effluent detection device

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Granted publication date: 20150708

Termination date: 20160407