CN204422550U - Multiplex disposable blood transfusion cross match blood test combiner - Google Patents
Multiplex disposable blood transfusion cross match blood test combiner Download PDFInfo
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- CN204422550U CN204422550U CN201520104646.4U CN201520104646U CN204422550U CN 204422550 U CN204422550 U CN 204422550U CN 201520104646 U CN201520104646 U CN 201520104646U CN 204422550 U CN204422550 U CN 204422550U
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Abstract
The utility model relates to medical experiment equipment, it is a kind of multiplex disposable blood transfusion cross match blood test combiner, it has transparent plastic small test tube, two application of sample pipes are provided with in the upper end of small test tube, it is characterized in that: be also provided with reagent adding and sample tap in the upper end of small test tube, at distance 4-10mm place, bottom surface, the bottom of small test tube, fork-join has a liquid suction pipe, and be furnished with a sampling viewer, it has suction pipe, the tail end of suction pipe is connected with a transparent plastic film cover, is connected with a manual gasbag after transparent plastic film cover; The utility model solves existing blood transfusion cross matching experiment and there is no special implement, adopts common test tube operation very complicated loaded down with trivial details, and the problem of easily makeing mistakes, a kind of multiplex disposable blood transfusion cross match blood test combiner is provided.
Description
Technical field
The utility model relates to medical experiment equipment, especially a kind of multiplex disposable blood transfusion cross match blood test combiner.
Background technology
Up to the present, it is all principles according to safe transfusion that situation of all-level hospitals Blood Transfusion Dept. will do blood transfusion cross matching experiment, the blood source (as A type RH positive patient need be transfused blood, the blood donor of the A type RH positive must be sought) of application abo blood group and RH blood group phase homotype, blood donor is separated with serum respectively with the blood cell of receptor, cross one another and be allowed to condition at reaction in test tube and observe its haemolysis agglutination phenomenon again, can judgement implement safe transfusion, namely carries out cross matching experiment.In the prior art, blood transfusion cross matching experiment also there is no special implement, common test tube is normally adopted to test, this experiment is as an a patient only defeated person-portion blood, then they should get primary and secondary two test tubes and add respectively: receptor's serum+blood donor's blood cell (supervisor): receptor's blood cell+blood donor's serum (secondary pipe).25-30 DEG C, within 5-10 minute, centrifugal taking-up visual inspection is with or without haemolysis and agglutination phenomenon, after pitching again, blood instillation slide use microscopic examination with or without agglutination phenomenon, finally report primary and secondary pipe with or without haemolysis and agglutination phenomenon, conclusion " conforms to " or " not conforming to ", judges the blood that could input this blood donor.
If when receptor needs massive transfusion (more than two person-portions) donor blood, cross matching experiment done before its blood transfusion is just more complicated.Such as y-bend is once needs six test tubes (because all will carry out cross matching experiment between every blood donor and receptor and between every blood donor), if receptor needs trident for the moment, that wants twelve earthly branches test tube, blood cell receptor and serum is added to application of sample is repeated multiple times from numbering, repeatedly every position application of sample, (only this task operator of application of sample to give with sample injector and often props up test tube to add blood cell and serum each once, then twelve earthly branches test tube wants application of sample 24 times, but also the mode that blood cell and serum push the wrong bit pairing to be added to supervisor and time pipe, its operation visible is very complicated loaded down with trivial details), when therefore needing the blood of multidigit blood donor, this cross-over experiment whole process is more complicated loaded down with trivial details and easily make mistakes.
For this reason, applicant had once applied for a kind of disposable blood transfusion cross matching experiment combiner on January 19th, 2011, and obtain utility model patent, but this combiner is only applicable to brine media blood mathcing test, now medical circle recommends cross match blood test to adopt cohesion amine medium method, former combiner is inconvenient to add semi-commercial (semiworks) production and pump drainage supernatant, and adopts the transparent plastic film capsule of small test tube side fork-join, observes tube manipulation inconvenience.
Summary of the invention
The purpose of this utility model will solve tradition blood transfusion cross matching experiment exactly and there is no special implement, common test tube is adopted to test, complicated operation is loaded down with trivial details, and easily make mistakes, and the combiner inconvenience of former utility model adds in test semi-commercial (semiworks) production and pump drainage supernatant, be not suitable for the test of cohesion amine medium method, observe sample, the problems such as inconvenient operation, provide a kind of multiplex disposable blood transfusion cross match blood test combiner.
The utility model is further improvement for former patented method and expansion, and proposes two schemes.
Scheme one: multiplex disposable match cross matching combiner, there is transparent plastic small test tube, two application of sample pipes are provided with in the upper end of small test tube, it is characterized in that: be also provided with in the upper end of small test tube and add agent sample tap, at distance 4-10mm place, bottom surface, the bottom of small test tube, fork-join has a liquid suction pipe, and is furnished with a sampler, and it has suction pipe, the tail end of suction pipe is connected with a transparent plastic film cover, is connected with a manual gasbag after transparent plastic film cover.
In scheme one, described two application of sample pipes are designed to that one is long and the other is short, or the cross sectional shape of two application of sample pipes is designed to two kinds of different shapes.
In scheme one, the length of described liquid suction pipe is the junction that upper port exceedes application of sample pipe and small test tube.
In scheme one, three and above small test tube is adopted to connect in a row.
Scheme two, multiplex disposable blood transfusion cross match blood test combiner, has (n+1)
2individual transparent plastic small test tube, n is blood donor's number, it is characterized in that: be designed with a test tube rack, and test tube rack is provided with (n+1) that square formation is arranged
2individual test tube jack, namely often row has n+1 test tube jack, do not show n+1 test tube hole, distance 4-10mm place, bottom surface, the bottom fork-join of described small test tube has a liquid suction pipe, all small test tubes are placed in the test tube jack of test tube rack one to one, and be equipped with application of sample chi and a sampling viewer of two different colours, described sampling viewer has suction pipe, the tail end of suction pipe is connected with a transparent plastic film cover, is connected with a manual gasbag after transparent plastic film cover.
In scheme two, connect in a row with the small test tube of the n+1 in a line.
In scheme two, the length of the liquid suction pipe of described small test tube bottom is lower than small test tube 10-20mm suitable for reading.
In scheme two, described two application of sample chis are redness blueness.
In scheme two, on every root application of sample chi, corresponding a row or column small test tube has n+1 well, or has slotted eye in the middle of every root application of sample chi, and the length of slotted eye is the spread length of a row or column small test tube.
The utility model structure is simple, low cost of manufacture, and single use is safe and reliable, application the utility model carries out blood transfusion cross match blood test, convenient and swift, not easily makes mistakes, particularly convenient in test with the adding and pump drainage supernatant of reagent, have special sampler, more convenient observation operation.The utility model is not only applicable to brine media blood mathcing test, can also be applicable to antibody screen seizure test and complementary antihuman globulin test, particularly can be used for condensing the blood mathcing test of amine medium.
Accompanying drawing explanation
Fig. 1 is the small test tube structural representation of the utility model structure one;
Fig. 2 is the sampling observation device structural representation of the utility model structure one;
Fig. 3 is the using state figure adopting the utility model structure one to carry out fork one test;
Fig. 4 is the small test tube structural representation of the utility model structure two;
Fig. 5 is the trident one test tube rack schematic diagram of the utility model structure two;
Fig. 6 is application of sample chi structure one schematic diagram of the utility model structure two;
Fig. 7 is application of sample chi structure two schematic diagram of the utility model structure two;
In figure: 1-square application of sample pipe, 2-add agent sample tap, 3-circular application of sample pipe, 4-liquid suction pipe, 5-small test tube, 6-manual gasbag, 7-transparent plastic film capsule, 8-suction pipe, 9-small test tube, 10-liquid suction pipe, 11-bungee, 12-test tube jack, 13-test tube rack, 14-red application of sample chi, 15-well, 16-blue application of sample chi, 17-slotted eye.
Embodiment
Embodiment 1
See Fig. 1, the utility model has transparent plastic small test tube 5, a square application of sample pipe 1 and a circular application of sample pipe 3 is provided with in the upper end of small test tube 5, (two application of sample pipes 1 described in the utility model, 3 also can be designed to that one is long and the other is short, or two application of sample pipes 1, the cross sectional shape of 3 is designed to two kinds of other different shapes), particularly: be also provided with in the upper end of small test tube and add agent sample tap 2, at distance 4-10mm place, bottom, the bottom of small test tube 5, fork-join has a liquid suction pipe 4, and be furnished with a sampling viewer, it has suction pipe 8, the tail end of suction pipe 8 is connected with a transparent plastic film capsule 7, a manual gasbag 6 is connected with after transparent plastic film capsule 7.
The length of liquid suction pipe 4 described in the present embodiment is the junction that upper port exceedes application of sample pipe and small test tube 5.
It is in a row in the present embodiment to be that employing three small test tubes 5 connect, and connector can be adopted to connect in a row, also can plastics preprocessing in a row, three small test tubes 5 and six roots of sensation application of sample pipe can a plastic shaping be structure as a whole.
The combiner that the present embodiment makes is used for fork one test when donor blood (receptor only need), and to carry out brine media blood mathcing test step as follows: it is in a row to get disposable blood transfusion cross matching experiment combiner three group, two groups of cross arrangements are divided by two combiner six roots of sensation application of sample pipes, and with bungee 11 combination of two together, wherein two rounded nose application of sample pipes are for adding blood cell suspension pipe, and two square spigot application of sample pipes are for healing pigging; Wherein a small test tube is supervisor, and another tubule is time pipe, and has own control pipe; Then in supervisor, add receptor's serum and blood donor's blood cell suspension, in secondary pipe, add receptor's blood cell suspension and blood donor's serum, in own control pipe, add patient self blood cell and serum.Carry out following operation again:
(1) above combiner three is managed the mixing of interior intersection thing and put into 25 DEG C of incubator reaction 5-10 minute;
(2) take out low-speed centrifugal 2 minutes, take out and observe supernatant respectively, with or without haemolysis, agglutination phenomenon;
(3) if without haemolysis, without agglutination phenomenon, then adopt sampler to be sucked in transparent plastic film capsule by the potpourri in small test tube, and examine under a microscope, with or without tiny agglutination phenomenon;
(4) all without haemolysis more than, without agglutination phenomenon, conclusion " meets " report, otherwise goes out " not meeting " report, can carry out first-class multi-fork one test of y-bend by that analogy.
As adopted cohesion amine medium rule to add low solion (LIM) 0.65ml again mix in often propping up in test tube 9 after application of sample in above-mentioned test, more often pipe to add the mixing of cohesion amine two centrifugal, then press 7,8,9 step end of operations in example 2.
Embodiment 2
See Fig. 4-7, two of the utility model structure is one group of combiner for trident one blood mathcing test, first the shaping small test tube as shown in Figure 49 of transparent plastic is adopted, the bottom distance bottom surface 4-10mm(of small test tube 9 is specially 6mm) place's fork-join has a liquid suction pipe 10, liquid suction pipe 10 is upwards arranged and 10-20mm(suitable for reading lower than small test tube 9 is specially 15mm mono-outlet), get four such small test tubes 9 to connect and in a rowly (can web member be adopted, as Connection Card is connected, all right plastics preprocessing time system is in a row, series of products can be made according to test needs, as 3 one rows, 5 one rows wait with applicable y-bend one, the first-class various blood mathcing test of four forks), prepare four row's small test tubes 9.Adopt plastic shaping test tube rack 13(as shown in Figure 5), it has 16 the test tube jacks 12 arranged in square formation.(four a line, four one row can make series of products according to test needs, as 3 a line, and 3 one row; 5 a line, 5 one row etc., pitch first-class various blood mathcing test with applicable y-bend one, four).Adopt plastic shaping one red application of sample chi 14(as Fig. 6) and a blue application of sample chi 16(as Fig. 7), red application of sample chi 14 has a row four wells 15, (well 15 is corresponding with the test tube jack on test tube rack 13 to be arranged, four wells 15 also can change slotted hole structure into), blue application of sample chi 16 has a slotted eye 17, and slotted eye 17 length is the length that a line four small test tubes 9 one are arranged.(slotted eye 17 also can change four rows into and add slotted hole structure).Last with example 1, make a sampling viewer (see Fig. 2 structure), namely form the blood mathcing test combiner of another kind of form of the present utility model.This combiner can make y-bend one, four pitch one, five fork first-class plurality of specifications, corresponding small test tube 9 is prepared n+1 company in a row.
Application the utility model combiner, it is as follows to carry out cohesion amine medium method trident one blood mathcing test step:
1. get four row's small test tube 9(and often arrange four), put into test tube rack 13;
2. receptor being compiled is No. 0, and it is 1,2, No. 3 that three blood donors compile respectively;
3. get red application of sample chi 14, be placed on the first row small test tube, corresponding the first row four small test tubes add two, No. 0 blood cell, more red application of sample chi 14 is moved to the second row, corresponding second row four small test tubes add two, No. 1 blood cell, in like manner add No. 2, No. 3 blood cells successively;
4. getting blue application of sample chi 16 is placed on the 1st row test tube, and corresponding first row four small test tubes add No. 0 serum two, bluer application of sample chi 16 is moved to secondary series, and corresponding secondary series four small test tubes add No. 1 serum two, in like manner adds No. 2, No. 3 serum successively;
5. in each small test tube, add low solion (LIM) 0.65ml, after mixing, in each small test tube, add cohesion amine aqueous solution again, (Polybene) solution two, and mix;
6. use special centrifugal machine, by each row's small test tube centrifugally operated 10 seconds (3400 turns/min), then adopt special drawing liquid pump to take out supernatant by the liquid suction pipe 10 on small test tube, and retain supernatant 0.1ml;
7. manually shake each row's test tube gently, estimate red blood cell with or without aggegation, as then reformed without aggegation;
8. add Resuspending(suspending agent) two droplets shake test tube mixing gently and observations, if aggegation is scattered in 60 seconds, representative condenses amine by Polybene to cause rejection to assemble match result to be harmonious; As aggegation is not scattered, be then the specific reaction that red cell antigens antibody combines, match result does not conform to;
9., as reaction can be coagulated, adopt sampling viewer to be sucked in transparent plastic film capsule by sample and use microscopic examination.
The combination of the utility model cross matching combiner is also applicable to brine media blood mathcing test antibody, Screen test and complementary antihuman globulin test.
Claims (9)
1. multiplex disposable blood transfusion cross match blood test combiner, there is transparent plastic small test tube, two application of sample pipes are provided with in the upper end of small test tube, it is characterized in that: be also provided with in the upper end of small test tube and add agent sample tap, at distance 4-10mm place, bottom surface, the bottom of small test tube, fork-join has a liquid suction pipe, and is furnished with a sampling viewer, and this sampling viewer has suction pipe, the tail end of suction pipe is connected with a transparent plastic film cover, is connected with a manual gasbag after transparent plastic film cover.
2. multiplex disposable blood transfusion cross match blood test combiner according to claim 1, is characterized in that: described two application of sample pipes are designed to that one is long and the other is short, or the cross sectional shape of two application of sample pipes is designed to two kinds of different shapes.
3. multiplex disposable blood transfusion cross match blood test combiner according to claim 1, is characterized in that: the length of described liquid suction pipe is the junction that upper port exceedes application of sample pipe and small test tube.
4. multiplex disposable blood transfusion cross match blood test combiner according to claim 1, is characterized in that: adopt three and above small test tube to connect in a row.
5. multiplex disposable blood transfusion cross match blood test combiner, has (n+1)
2individual transparent plastic small test tube, n is blood donor's number, it is characterized in that: be designed with a test tube rack, and test tube rack is provided with (n+1) that square formation is arranged
2individual test tube jack, namely often row has n+1 test tube jack, often show n+1 test tube hole, distance 4-10mm place, bottom surface, the bottom fork-join of described small test tube has a liquid suction pipe, all small test tubes are placed in the test tube jack of test tube rack one to one, and be equipped with application of sample chi and a sampling viewer of two different colours, described sampling viewer has suction pipe, the tail end of suction pipe is connected with a transparent plastic film cover, is connected with a manual gasbag after transparent plastic film cover.
6. multiplex disposable blood transfusion cross match blood test combiner according to claim 5, is characterized in that: connect in a row with the small test tube of the n+1 in a line.
7. multiplex disposable blood transfusion cross match blood test combiner according to claim 5, is characterized in that: the length of the liquid suction pipe of described small test tube bottom establishes a liquid outlet lower than small test tube lower 10-20mm place suitable for reading.
8. multiplex disposable blood transfusion cross match blood test combiner according to claim 5, is characterized in that: described two application of sample chis are redness blueness.
9. the multiplex disposable blood transfusion cross match blood test combiner according to claim 5 or 8, it is characterized in that: on every root application of sample chi, corresponding a row or column small test tube has n+1 well, or slotted eye is had in the middle of every root application of sample chi, the length of slotted eye is the spread length of a row or column small test tube.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520104646.4U CN204422550U (en) | 2015-02-13 | 2015-02-13 | Multiplex disposable blood transfusion cross match blood test combiner |
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| CN201520104646.4U CN204422550U (en) | 2015-02-13 | 2015-02-13 | Multiplex disposable blood transfusion cross match blood test combiner |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104614538A (en) * | 2015-02-13 | 2015-05-13 | 刘大基 | Multifunctional disposable transfusion and cross blood-matching test combiner |
| CN105548581A (en) * | 2015-12-04 | 2016-05-04 | 漯河医学高等专科学校 | Gel for cross matching in blood transfusion, preparation method and experiment combiner |
-
2015
- 2015-02-13 CN CN201520104646.4U patent/CN204422550U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104614538A (en) * | 2015-02-13 | 2015-05-13 | 刘大基 | Multifunctional disposable transfusion and cross blood-matching test combiner |
| CN105548581A (en) * | 2015-12-04 | 2016-05-04 | 漯河医学高等专科学校 | Gel for cross matching in blood transfusion, preparation method and experiment combiner |
| CN105548581B (en) * | 2015-12-04 | 2017-09-22 | 漯河医学高等专科学校 | One kind blood transfusion cross matching gel, preparation method and experimental group clutch |
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