CN204314218U

CN204314218U - A kind of Novel ATP fluorescence microbial rapid detection instrument

Info

Publication number: CN204314218U
Application number: CN201420716112.2U
Authority: CN
Inventors: 侯玉柱; 柯润辉; 尹建军; 宋全厚; 田雨
Original assignee: China National Research Institute of Food and Fermentation Industries
Current assignee: China National Research Institute of Food and Fermentation Industries
Priority date: 2014-11-25
Filing date: 2014-11-25
Publication date: 2015-05-06
Anticipated expiration: 2024-11-25

Abstract

The utility model discloses a kind of Novel ATP fluorescence microbial rapid detection instrument.It comprises ATP fluorescent microorganism detector body, is provided with sampling device in ATP fluorescent microorganism detector body; Sampling device is provided with the sample introduction tank of the concentric circles setting that 2 internal diameters do not wait.The utility model tool has the following advantages: the concentric sample introduction tank that (1) two internal diameter is different, both can place little sample introduction cup, smears the direct mensuration needs of the bacteria suspension of high bacterial content for routine; Large sample introduction cup can be changed again into, sample for low bacterial content is with film to measure after miillpore filter enrichment method, food enterprise can be helped to monitor material surface sanitary condition, the low bacterial content sample tests demands such as purging system water can be met again, avoid the filter membrane after concentrating with miillpore filter carry out secondary wash-out again sample detection time inoculating microbe secondary pollution risk.(2) based on ATP bioluminescence technology, simple to operate, sample introduction is easy, reliable results, more can meet the actual demand of enterprise.

Description

A kind of Novel ATP fluorescence microbial rapid detection instrument

Technical field

The utility model relates to a kind of Novel ATP fluorescence microbial rapid detection instrument, belongs to microorganism detection field, food safety detection aspect.

Background technology

For further condensed food production overall process supervision, improve the food enterprises quality and safety control ability, national health State Family Planning Commission has issued " national food safety standard food production general sanitary specification " (GB14881-2013).This specification is fundamental prerequisite and the hygienic requirements of food production, and the refinement Sum decomposition of the requirement such as food production, factory building layout, installations and facilities, personnel's health of proposition is the important means that manufacturing enterprise ensures food security.Hygienic practice enforced in 1 day June in 2014.

The sanitation monitoring of food production process can effectively prevent and reduce the possibility of health contamination accident generation, improve food safety standard, there is the shortcoming detecting hysteresis quality in traditional microorganism detection method GB4789.2-2010, the sanitary condition of health critical control point cannot be monitored in real time, hazard (Hazard AnalysisCritical Control Point, HACCP) the most effective system is controlled as food production, the support of Fast Detection Technique is needed more effectively to play a role, ATP (adenosine triphyosphate) fluorescence microbial rapid detection technology has easy and simple to handle, rapid sensitive, with low cost, the advantages such as environmental protection, can as effective support of HACCP system, timely identification exogenous microbial pollutes, and can rapid evaluation effect of sanitary treatment whether up to standard.

ATP fluorescence microbial rapid detection instrument basic source is in the market in import, and structure is also similar, is all and the sanitary condition of semar technique in conjunction with Fast Measurement material surface.Existing microorganism examines the method that instrument detects low bacteria containing amount sample soon, it is all the mode measured after adopting filter membrane enrichment method, sample after general miillpore filter concentrates carries out secondary wash-out, then sample detection, and too much operation steps can bring the risk of inoculating microbe secondary pollution.

Utility model content

The purpose of this utility model is to provide a kind of Novel ATP fluorescence microbial rapid detection instrument, the utility model both may be used for high bacteria containing amount fluid sample direct injected and had detected, may be used for again low bacteria containing amount example enrichment to measure, and the utility model can be used for the miillpore filter of enrichment of bacterial without the need to wash-out, directly put into sample introduction cup and add reagent detection; Detector is based on ATP bioluminescence technology, simple to operate, sample introduction is easy, reliable results, more can meet the actual demand of enterprise.

The Novel ATP fluorescence microbial rapid detection instrument that the utility model provides, it comprises ATP fluorescent microorganism detector body, is provided with sampling device in described ATP fluorescent microorganism detector body;

Described sampling device is provided with the sample introduction tank that at least 2 internal diameters do not wait.

Above-mentioned detector, described sampling device is provided with the sample introduction tank that 2 internal diameters do not wait.

Above-mentioned detector, 2 described sample introduction tank are that concentric circles is arranged, and the setting of center of circle circle ensure that the homogeny in light signal collection region, thus ensures the repeatability and the reliability that detect data.

Above-mentioned detector, the diameter as the described sample introduction tank of inner circle is 9mm;

Diameter as the described sample introduction tank of cylindrical is 20mm.

The little sample introduction cup that in the utility model ATP fluorescence microbial rapid detection instrument, the described sample introduction tank of inner circle can be 8.5mm with external diameter, internal diameter is 6.5mm coordinates, and the material of little sample introduction cup is transparent plastic, and little sample introduction cup is single use; The large sample introduction cup that the sample introduction tank of cylindrical can be 18mm with external diameter, internal diameter is 16mm coordinates, and the material of large sample introduction cup can be clear glass, reusable.

Use course of work during the utility model bacterial detection sum as follows:

When detecting the sample of high bacterial content, directly sample liquid to be checked can be added little sample introduction cup, little sample introduction cup is positioned in the described sample introduction tank of described inner circle, push the ATP fluorescent value measuring testing sample in ATP fluorescent microorganism detector body.

During the sample detection of low bacterial content, by the sample of low bacterial content after miillpore filter enrichment method, miillpore filter is directly put into bottom large sample introduction cup, after directly adding fluorescent reagent on film, large sample introduction cup is positioned in the sample introduction tank of described cylindrical, pushes in ATP fluorescent microorganism detector body and measure.

Compared with prior art, the utility model tool has the following advantages:

(1) can dual-use, the sample introduction tank that concentric two internal diameters are different, both can place little sample introduction cup, smeared the direct mensuration needs of the bacteria suspension of high bacterial content for routine; Large sample introduction cup can be changed again into, sample for low bacterial content is with film to measure after miillpore filter enrichment method, namely food enterprise can be helped to monitor material surface sanitary condition, the low bacterial content sample tests demands such as purging system (CIP) water can be met again, avoid the filter membrane after concentrating with miillpore filter carry out secondary wash-out again sample detection time inoculating microbe secondary pollution risk.

(2) detector is based on ATP bioluminescence technology, simple to operate, sample introduction is easy, reliable results, more can meet the actual demand of enterprise.

Accompanying drawing explanation

Fig. 1 is the structural representation of the utility model ATP fluorescence microbial rapid detection instrument.

In figure, mark is as follows:

1 ATP fluorescent microorganism detector body, 2 sampling devices, 3 sample introduction tank I, 4 sample introduction tank II.

Embodiment

Below in conjunction with accompanying drawing, the utility model is further described, but the utility model is not limited to following examples.

As shown in Figure 1, be the utility model ATP fluorescence microbial rapid detection instrument, it comprises ATP fluorescent microorganism detector body 1, ATP fluorescent microorganism detector body 1 and is connected with sampling device 2.Sampling device 2 is provided with the sample introduction tank that 2 internal diameters are respectively the concentric circles setting of 9mm and 20mm, be labeled as sample introduction tank I 3 and sample introduction tank II 4 successively, the concentric circles of sample introduction tank I 3 and sample introduction tank II 4 arranges the homogeny that ensure that light signal collection region, thus ensures the repeatability and the reliability that detect data.

The little sample introduction cup that in the utility model ATP fluorescence microbial rapid detection instrument, sample introduction tank I 3 can be 8.5mm with external diameter, internal diameter is 6.5mm coordinates, and the material of little sample introduction cup is transparent plastic, and little sample introduction cup is single use; The large sample introduction cup that sample introduction tank II 4 can be 18mm with external diameter, internal diameter is 16mm coordinates, and the material of large sample introduction cup can be clear glass, reusable.

Use course of work during the utility model detection ATP fluorescence microorganism as follows:

One, the sample detection of high bacterial content:

When detecting the sample of high bacterial content, directly sample liquid to be checked can be added little sample introduction cup, little sample introduction cup is positioned in sample introduction tank I 3, push the ATP fluorescent value measuring testing sample in ATP fluorescent microorganism detector body.

Two, the sample detection of low bacterial content:

During the sample detection of low bacterial content, by the sample of low bacterial content after miillpore filter enrichment method, miillpore filter is directly put into bottom large sample introduction cup, after directly adding fluorescent reagent on film, large sample introduction cup is positioned in sample introduction tank II 4, pushes in ATP fluorescent microorganism detector body and measure.

Claims

1. a Novel ATP fluorescence microbial rapid detection instrument, it comprises ATP fluorescent microorganism detector body, is provided with sampling device in described ATP fluorescent microorganism detector body; It is characterized in that:

2. detector according to claim 1, is characterized in that: described sampling device is provided with the sample introduction tank that 2 internal diameters do not wait.

3. detector according to claim 2, is characterized in that: 2 described sample introduction tank are that concentric circles is arranged.

4. detector according to claim 3, is characterized in that: the diameter as the described sample introduction tank of inner circle is 9mm;

Diameter as the described sample introduction tank of cylindrical is 20mm.