CN204214703U - Shaking table device - Google Patents
Shaking table device Download PDFInfo
- Publication number
- CN204214703U CN204214703U CN201420599817.0U CN201420599817U CN204214703U CN 204214703 U CN204214703 U CN 204214703U CN 201420599817 U CN201420599817 U CN 201420599817U CN 204214703 U CN204214703 U CN 204214703U
- Authority
- CN
- China
- Prior art keywords
- shaking table
- rocker
- drive motor
- sample
- web member
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000005096 rolling process Methods 0.000 claims description 15
- 239000007788 liquid Substances 0.000 abstract description 10
- 230000008014 freezing Effects 0.000 description 18
- 238000007710 freezing Methods 0.000 description 18
- 238000006467 substitution reaction Methods 0.000 description 16
- 238000000034 method Methods 0.000 description 9
- 241001131688 Coracias garrulus Species 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model relates to a kind of shaking table device, and it comprises shaking table, sample cavity chamber cap, table driving device for carrying sample; Described table driving device is arranged on described sample chamber and covers, and is connected with described shaking table by a web member passing described sample cavity chamber cap, for driving described web member to move, thus drives described shaking table to shake.The shaking table device that the utility model provides, by coordinating of table driving device and shaking table, can increase the seepage velocity of replacement liquid, greatly shorten the replacement time of replacement liquid.
Description
Technical field
The utility model relates to Bioexperiment equipment technical field, particularly a kind of shaking table device.
Background technology
Freezing substitution, as one of the method for biological electron microscope sample preparation, is widely used in the research of cell ultrastructure at present.Conventional method for making sample carries out chemistry at normal temperatures to fix and dehydration, easily cause some soluble component and the macromolecular loss of part biological and redistribution, cause cyto-architectural change, thus can not meet the requirement of people research organization and cell ultrastructure on a molecular scale.The ultimate principle of freezing substitution method is, at low temperatures with organic solvent by the replacement slowly of the solid water in the sample of snap frozen (as high pressure is freezing) out, simultaneously auxiliaryly to fix with chemistry, at electric Microscopic observation after high temperature or low temperature embedding, polymerization, section, heavy metal dyeing.The method both can preserve good ultrastructure during cell native state, was again important method prepared by Electron Microscopic Sample.
At present, freezing substitution instrument has business-like product, the robotization of whole freezing substitution process implementation, and when can accurately control, temperature control.But because sample remains static in freezing substitution instrument, in organic solvent and sample, the replacing velocity of water is slow at low temperatures, cause the experimental period of freezing substitution very long, usual needs 5 ~ 7 days, sample for the infiltration of the difficulty such as plant, yeast then needs the time more grown, this not only makes the service efficiency of instrument low, more greatly limit the speed of experiment progress.Therefore, when not affecting substitution effect, shortening the time of freezing substitution, becoming problem demanding prompt solution.
Utility model content
One of the purpose of this utility model is to provide a kind of seepage velocity that can increase replacement liquid, shortens the shaking table device of replacement liquid replacement time.
According to an aspect of the present utility model, providing a kind of shaking table device, comprising the shaking table for carrying sample, sample cavity chamber cap, table driving device; Described table driving device is arranged on described sample chamber and covers, and is connected with described shaking table by a web member passing described sample cavity chamber cap, for driving described web member to move, thus drives described shaking table to shake.
Further, described table driving device comprises drive motor, motor rack and cam; Described drive motor is fixed on the top board of described sample cavity chamber cap by motor rack, and described cam is connected with described drive motor and described web member respectively, and described drive motor drives described cam rotate and then drive described web member to move up and down.
Further, described shaking table comprises shaking table rocker, shaker stent and shaking table base; Described shaking table rocker is connected by axle with described shaker stent; Described shaking table base is fixedly connected with described shaker stent.
Further, the axle of described shaking table rocker and described shaker stent is equipped with torsion spring, for resetting.
Further, described table driving device comprises drive motor; Described drive motor is fixed on the top board of described sample cavity chamber cap; The axle of described drive motor assembles described web member, and the other end of described web member assembles a rolling member, and described rolling member contacts with described shaking table; Described drive motor drives described web member to rotate, and described rolling member is rolled around shaking table, thus drives described shaking table to shake.
Further, described shaking table comprises shaking table rocker, shaking table base, shaking table rocker center bearing bracket and shaking table rocker center bearing bracket universal joint; Described shaking table rocker center bearing bracket is fixed on described shaking table base, and be connected with described shaking table rocker by shaking table rocker center bearing bracket universal joint, when described rolling member rolls around described shaking table rocker, described shaking table rocker is shaken around described shaking table rocker center bearing bracket with sample.
Further, described web member is motor connection lever, and described rolling member is roller, and the part that described motor connection lever contacts with described shaking table rocker is provided with described roller.
Further, described shaking table comprises shaking table rocker, shaker stent and shaking table base; Described shaking table rocker is connected by axle with described shaker stent; Described shaking table base is fixedly connected with described shaker stent; Described axle is provided with driven sprocket.
Further, described table driving device comprises drive motor and motor rack; Described drive motor is fixed on described motor rack, and described motor rack is fixed on the top board of described sample cavity chamber cap, the axle of described drive motor assembles a drive sprocket; Described web member is chain, and described chain is connected with described drive sprocket and driven sprocket respectively; When described drive motor rotates, drive sprocket drives described chain movement, and chain drives again described driven sprocket to rotate, thus makes described shaking table rocker can do rolling movement in the vertical direction.
Further, described shaking table rocker configures some spring clamps, for clamping sample hose.
The shaking table device that the utility model provides, by coordinating of table driving device and shaking table, can increase the seepage velocity of replacement liquid, greatly shorten the replacement time of replacement liquid.
Accompanying drawing explanation
The structural representation of the shaking table device that Fig. 1 provides for the utility model embodiment one;
The structural representation of the shaking table device that Fig. 2 provides for the utility model embodiment two;
The structural representation of the shaking table device that Fig. 3 provides for the utility model embodiment three.
Embodiment
For enabling above-mentioned purpose of the present utility model, feature and advantage become apparent more, are described in further detail the utility model below in conjunction with the drawings and specific embodiments.
The utility model provides a kind of shaking table device, comprises the shaking table for carrying sample, sample cavity chamber cap and table driving device.Table driving device is arranged on sample chamber and covers, and is connected with shaking table by a web member passing sample cavity chamber cap, for driving web member to move, thus drives shaking table shake.Below in conjunction with specific embodiment the shaking table device that the utility model provides is applied on freezing substitution instrument and is specifically described.
Embodiment one
See Fig. 1, the table driving device in the shaking table device that the utility model embodiment provides comprises drive motor 1, motor rack 3 and cam 2; Drive motor 1 is fixed on the top board 4 of sample cavity chamber cap 5 by motor rack 3.Cam 2 is connected with drive motor 1 and web member respectively.Drive motor 1 is with moving cam 2 to rotate and then is with follower link to move up and down.Web member is specifically as follows a connecting rod 6.Top board 4 is transparent, in order to observe the situation of sample chamber.Sample cavity chamber cap 5, for covering cryogenic chamber 12, makes the pollution that it keeps low temperature environment and prevents extraneous aqueous vapor.
Shaking table comprises shaking table rocker 7, shaker stent 9 and shaking table base 8.Shaking table rocker 7 is connected by axle with shaker stent 9; Shaking table base 8 is fixedly connected with shaker stent 9.Shaking table rocker 7 and the axle of shaker stent 9 are equipped with torsion spring (not shown), for resetting.Shaking table rocker 7 configures some spring clamps 10, for clamping sample hose 11.
The use procedure of this shaking table device is exemplified below: open freezing substitution instrument, adds liquid nitrogen, is chilled in advance lower than-90 DEG C; Low temperature shaking table is put into sample cavity precooling; Get 1.5ml centrifuge tube, add about 1ml replacement liquid (1-2% osmic acid is dissolved in the acetone containing 5% ultrapure water), cool in liquid nitrogen; Centrifuge tube containing sample being transferred to the good freezing substitution instrument of precooling substitutes indoor, adds the replacement liquid cooled in advance; Be fixed on the fixing spring folder 10 of shaking table sample; Cover sample cavity chamber cap 5, fastening, make connecting rod withstand on shaking table rocker 7; Open motor control switch, set motor speed, cam 2 drive link 6, connecting rod moves up and down, and the one end that press against shaking table rocker 7 fluctuates, and shaking table rocker 7 is shaken, and the solution in centrifuge tube constantly stirs; Set freezing substitution instrument parameter, arrange and run freezing substitution program; Complete Deng freezing substitution program, close shaking table switch, open sample chamber top cover, take out sample, close freezing substitution instrument.
Embodiment two
See Fig. 2, the table driving device in the shaking table device that the utility model embodiment provides comprises drive motor 1; Drive motor 1 is fixed on the top board 4 of sample cavity chamber cap 5.Top board 4 is transparent, in order to observe the situation of sample chamber.Sample cavity chamber cap 5, for covering cryogenic chamber 12, makes the pollution that it keeps low temperature environment and prevents extraneous aqueous vapor.Assembling connecting piece on the axle of drive motor 1.Web member can be specifically motor connection lever 13.The other end of motor connection lever 13 assembles a rolling member, i.e. roller 14.Shaking table comprises shaking table rocker 7, shaking table base 8, shaking table rocker center bearing bracket 15 and shaking table rocker center bearing bracket universal joint 16.Shaking table rocker center bearing bracket 15 is fixed on shaking table base 8, and is connected with shaking table rocker 7 by shaking table rocker center bearing bracket universal joint 16.Shaking table rocker 7 configures some spring clamps 10, for clamping sample hose 11.During work, roller 14 contacts with shaking table rocker 7.Drive motor 1 rotates and drives motor connection lever 13 360 ° of rotations in the horizontal direction, and roller 14 rolls across at shaking table rocker 7 edge, and shaking table rocker 7 is shaken around shaking table rocker center bearing bracket 15 with sample.
Embodiment three
As shown in Figure 3, the shaking table in the shaking table device that provides of the utility model embodiment comprises shaking table rocker 7, shaker stent 9 and shaking table base 8.Shaking table rocker 7 is connected by axle with shaker stent 9, and the side of this axle is provided with driven sprocket 19.Shaking table base 8 is fixedly connected with shaker stent 9.Shaking table rocker 7 configures some spring clamps 10, for clamping sample hose 11.
Table driving device comprises drive motor 1 and motor rack 3.Drive motor is fixed on motor rack 3.Motor rack 3 is fixed on the top board 4 of sample cavity chamber cap 5.Top board 4 is transparent, in order to observe the situation of sample chamber.Sample cavity chamber cap 5, for covering cryogenic chamber 12, makes the pollution that it keeps low temperature environment and prevents extraneous aqueous vapor.The axle of drive motor 1 assembles a drive sprocket 17.Web member is chain 18.Chain 18 is connected with drive sprocket 17 and driven sprocket 19 respectively; When drive motor 1 rotates, drive sprocket 17 drives chain 18 to move, and chain 18 drives again driven sprocket 19 to rotate, thus makes shaking table rocker 7 can do rolling movement in the vertical direction.Control forward and reverse operation of drive motor 1, the change of rotational angle and speed, can make shaking table rocker 7 with various ways such as different amplitude and velocity interpolation shake, rollings.
The shaking table device that the utility model embodiment provides, has following beneficial effect:
If 1 is applied in freezing substitution instrument field, the low temperature shaking table device that snap frozen substitutes can be realized, can greatly provide freezing substitution efficiency.The utility model shaking table device is divided into low temperature part and room temperature part.Low temperature part mainly shaking table, and be not suitable for being placed on the table driving device (such as, motor and power supply) worked in low temperature environment and be placed on outside low temperature cavity chamber cap.This design both can be whole device working stability, decreased again quantity and the volume of parts in low temperature sample room to greatest extent, saved space.
2, change the form of the kind of motor and the method for operation, connected mode, shaking table, can realize that shaking table is multi-form, the motion of friction speed amplitude, be applicable to different application field and sample type.Whole apparatus design structure is simple, easy to operate, with low cost.
3, this shaking table device through the adjustment of part dimension and shape, can use as instrument separately, can be arranged on again on business-like freezing substitution instrument and use.Both can be used as the instrument that in sample for use in transmitted electron microscope technology of preparing, snap frozen substitutes, can be applicable to again that other needs are severe at low temperature, high temperature, high humidity, high salt etc., environment that shaking table control system is put into by extreme environment and being not suitable for, the operating process such as shake, mixing, stirring, infiltration of sample, product or raw material uses.
Be described in detail the utility model above, apply specific case herein and set forth principle of the present utility model and embodiment, the explanation of above embodiment just understands method of the present utility model and core concept thereof for helping; Meanwhile, for one of ordinary skill in the art, according to thought of the present utility model, all will change in specific embodiments and applications, in sum, this description should not be construed as restriction of the present utility model.
Claims (10)
1. a shaking table device, is characterized in that, comprising:
For carrying shaking table, sample cavity chamber cap, the table driving device of sample; Described table driving device is arranged on described sample chamber and covers, and is connected with described shaking table by a web member passing described sample cavity chamber cap, for driving described web member to move, thus drives described shaking table to shake.
2. shaking table device according to claim 1, is characterized in that, described table driving device comprises drive motor, motor rack and cam; Described drive motor is fixed on the top board of described sample cavity chamber cap by motor rack, and described cam is connected with described drive motor and described web member respectively, and described drive motor drives described cam rotate and then drive described web member to move up and down.
3. shaking table device according to claim 2, is characterized in that, described shaking table comprises shaking table rocker, shaker stent and shaking table base; Described shaking table rocker is connected by axle with described shaker stent; Described shaking table base is fixedly connected with described shaker stent.
4. shaking table device according to claim 3, is characterized in that, the axle of described shaking table rocker and described shaker stent is equipped with torsion spring, for resetting.
5. shaking table device according to claim 1, is characterized in that, described table driving device comprises drive motor; Described drive motor is fixed on the top board of described sample cavity chamber cap; The axle of described drive motor assembles described web member, and the other end of described web member assembles a rolling member, and described rolling member contacts with described shaking table; Described drive motor drives described web member to rotate, and described rolling member is rolled around shaking table, thus drives described shaking table to shake.
6. shaking table device according to claim 5, is characterized in that, described shaking table comprises shaking table rocker, shaking table base, shaking table rocker center bearing bracket and shaking table rocker center bearing bracket universal joint; Described shaking table rocker center bearing bracket is fixed on described shaking table base, and be connected with described shaking table rocker by shaking table rocker center bearing bracket universal joint, when described rolling member rolls around described shaking table rocker, described shaking table rocker is shaken around described shaking table rocker center bearing bracket with sample.
7. shaking table device according to claim 6, is characterized in that, described web member is motor connection lever, and described rolling member is roller, and the part that described motor connection lever contacts with described shaking table rocker is provided with described roller.
8. shaking table device according to claim 1, is characterized in that, described shaking table comprises shaking table rocker, shaker stent and shaking table base; Described shaking table rocker is connected by axle with described shaker stent; Described shaking table base is fixedly connected with described shaker stent; Described axle is provided with driven sprocket.
9. shaking table device according to claim 8, is characterized in that, described table driving device comprises drive motor and motor rack; Described drive motor is fixed on described motor rack, and described motor rack is fixed on the top board of described sample cavity chamber cap, the axle of described drive motor assembles a drive sprocket; Described web member is chain, and described chain is connected with described drive sprocket and driven sprocket respectively; When described drive motor rotates, drive sprocket drives described chain movement, and chain drives again described driven sprocket to rotate, thus makes described shaking table rocker can do rolling movement in the vertical direction.
10. the shaking table device according to any one of claim 3,6 or 8, is characterized in that, described shaking table rocker configures some spring clamps, for clamping sample hose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420599817.0U CN204214703U (en) | 2014-10-16 | 2014-10-16 | Shaking table device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420599817.0U CN204214703U (en) | 2014-10-16 | 2014-10-16 | Shaking table device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204214703U true CN204214703U (en) | 2015-03-18 |
Family
ID=52983368
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420599817.0U Expired - Fee Related CN204214703U (en) | 2014-10-16 | 2014-10-16 | Shaking table device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204214703U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104330290B (en) * | 2014-10-16 | 2017-04-05 | 中国科学院生物物理研究所 | Shaking table device |
| CN107913626A (en) * | 2016-10-10 | 2018-04-17 | 深圳开立生物医疗科技股份有限公司 | A kind of test tube arranges evenly mixing device |
-
2014
- 2014-10-16 CN CN201420599817.0U patent/CN204214703U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104330290B (en) * | 2014-10-16 | 2017-04-05 | 中国科学院生物物理研究所 | Shaking table device |
| CN107913626A (en) * | 2016-10-10 | 2018-04-17 | 深圳开立生物医疗科技股份有限公司 | A kind of test tube arranges evenly mixing device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150318 Termination date: 20191016 |
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| CF01 | Termination of patent right due to non-payment of annual fee |