CN204174197U - A kind of magnetic bead and the separatory magnetic head of binding substances thereof - Google Patents
A kind of magnetic bead and the separatory magnetic head of binding substances thereof Download PDFInfo
- Publication number
- CN204174197U CN204174197U CN201420382458.3U CN201420382458U CN204174197U CN 204174197 U CN204174197 U CN 204174197U CN 201420382458 U CN201420382458 U CN 201420382458U CN 204174197 U CN204174197 U CN 204174197U
- Authority
- CN
- China
- Prior art keywords
- binding substances
- magnetic
- inner sleeve
- magnetic bead
- separatory
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 239000011324 bead Substances 0.000 title claims abstract description 52
- 239000000126 substance Substances 0.000 title claims abstract description 47
- 238000001179 sorption measurement Methods 0.000 claims abstract description 33
- 230000000694 effects Effects 0.000 claims description 4
- 239000000696 magnetic material Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000002028 Biomass Substances 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 8
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000000926 separation method Methods 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 230000001276 controlling effect Effects 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- BGPVFRJUHWVFKM-UHFFFAOYSA-N N1=C2C=CC=CC2=[N+]([O-])C1(CC1)CCC21N=C1C=CC=CC1=[N+]2[O-] Chemical compound N1=C2C=CC=CC2=[N+]([O-])C1(CC1)CCC21N=C1C=CC=CC1=[N+]2[O-] BGPVFRJUHWVFKM-UHFFFAOYSA-N 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 238000011953 bioanalysis Methods 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000003245 working effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of magnetic bead and the separatory magnetic head of binding substances thereof, belong to magnetic bead and binding substances separation Instrument technology field thereof.This device comprises shell and adsorption magnet, described shell is made up of jacket and inner sleeve, described inner sleeve can move up and down in described overcoat, and described adsorption magnet is arranged in described overcoat and described inner sleeve, and can in described overcoat with described inner sleeve up and down or vertical movement.This device can complete absorption and the releasing operation of magnetic bead and binding substances thereof on demand smoothly, simply easily, and can available protecting magnetic head itself and culture dish and experiment sample wherein.
Description
Technical field
The utility model belongs to magnetic bead and binding substances separation Instrument technology field thereof, is specifically related to a kind of magnetic bead and the separatory magnetic head of binding substances thereof, be used alone or as instrument one parts use.
Background technology
As magnetic bead and the separatory magnetic head of binding substances thereof, there are electro-magnet and permanent magnet as material, in use artificial or power operation step is too much, greatly have impact on the level of automation of bio-analysis instrument, efficiency and quality.Such as, after magnetic head adsorbs magnetic bead and binding substances thereof, use during permanent magnet and just need to add plastic jacket on magnet, when magnetic bead and binding substances thereof will be discharged, just need magnet to extract out from plastic jacket, under the prerequisite not having adsoption-magnetic field, collect magnetic bead and binding substances product thereof.This operation is more loaded down with trivial details, needs to be extracted out from plastic jacket by magnet or insert; Magnet inserted simultaneously or extract plastic jacket out, easily damage plastic jacket and damage magnetic bead and binding substances thereof, directly affecting the sensitivity of biological detection and the quality of ravin.
When using conventional magnetic heads to be separated the target cell of magnetic bead combination, because the general idea of operation, the lid taking away Tissue Culture Dish may be forgotten, when magnetic head attempts culture tank internal adsorption magnetic bead or magnetic bead binding substances, culture dish lid and sample just directly possible and magnet extrude, and such culture dish lid is just likely damaged by pressure by magnetic head, both destroys magnetic head, damage again the sample in culture dish, so need a method prevented.
Utility model content
The purpose of this utility model is to provide a kind of absorption and release magnetic bead and the magnetic field working method of binding substances thereof, and can in the process of operation, the device of available protecting magnetic head itself and culture dish used and the biological specimen that will detect.
The technical scheme that the utility model adopts is:
A kind of magnetic bead and the separatory magnetic head of binding substances thereof, comprise shell and adsorption magnet, described shell is made up of jacket and inner sleeve, described inner sleeve can move up and down in described overcoat, described adsorption magnet is arranged in described overcoat and described inner sleeve, and can move up and down in described overcoat and described inner sleeve.
Further, described binding substances refers to the biomass cells and biomacromolecule that combine with magnetic bead.
Preferably, the diameter of described inner sleeve is less than the diameter of described overcoat.
Further, described inner sleeve is connected by pressure protect key with described overcoat.
Further, in described inner sleeve, permanent magnet is installed.
Preferably, the bottom of described inner sleeve is opening form or closed.
Preferably, the subsidiary material of described shell and inner casing are non-magnetic material.
Further, the bottom of described inner sleeve is set with plastic jacket, and described plastic jacket is directly adsorbed onto magnet for avoiding magnetic bead and binding substances thereof.
Further, moving up and down of described adsorption magnet can be reached by the effect of external magnetic field.
Further, it is by the top or the bottom extraneous magnetic field that are added in magnetic head, can utilize different pole orientation that adsorption magnet moves, and acts on inner adsorption magnet and makes it to reach and move up and down.
The utility model has the following advantages:
This apparatus structure is simple, production cost is low, this device can under instrument or artificial control, the magnetic field of conversion absorption and release magnetic bead and binding substances thereof effectively, reach the object of collecting magnetic bead and binding substances thereof, easy to operate, in order to change absorption and discharge the troublesome operation step done in magnetic field before decreasing; This device has protection biological specimen and magnetic head itself is without prejudice simultaneously, for device gives a warning condition, be convenient to Timeliness coverage check correlated condition in biological sample analysis process and operation whether correct.
Accompanying drawing explanation
A kind of magnetic bead that Fig. 1 provides for the utility model embodiment 1 and the separatory magnetic head of binding substances thereof are in the structural representation (magnet is away from bottom inner sleeve) during off working state;
A kind of magnetic bead that Fig. 2 provides for the utility model embodiment 1 and the separatory magnetic head of binding substances thereof are in the structural representation (magnetic head is in the situation of arresting magnetic bead and binding substances thereof) during readiness for operation;
A kind of magnetic bead that Fig. 3 provides for the utility model embodiment 1 and the separatory magnetic head of binding substances thereof are in the structural representation (magnetic bead and binding substances thereof are attracted to the surface of plastic jacket) during absorption magnetic bead;
A kind of magnetic bead that Fig. 4 provides for the utility model embodiment 1 and the separatory magnetic head of binding substances thereof are in the structural representation (away from bottom, magnetic bead and binding substances thereof are released adsorption magnet) during release adsorptive;
A kind of magnetic bead that Fig. 5 provides for the utility model embodiment 1 and the separatory magnetic head of binding substances thereof when pressure anomaly protection magnetic head and biological specimen not damaged time structural representation.
Embodiment
Below in conjunction with drawings and embodiments, the utility model is described in further detail.
Embodiment 1
Referring to figs. 1 through Fig. 5, a kind of magnetic bead that the present embodiment provides and the separatory magnetic head of binding substances thereof, comprise shell and adsorption magnet 1 (magnetic head).Shell is made up of overcoat 2 and inner sleeve 3, and inner sleeve 3 vertically can move in overcoat 2, and the diameter of inner sleeve 3 is less than the diameter of overcoat 2, and the bottom of inner sleeve 3 is opening form, and its opening is slightly less than the diameter of adsorption magnet 1, and the material of shell is non-magnetic material.In the present embodiment, the diameter of overcoat 2 is 0.8mm, and its length is 40mm, and the opening diameter of inner sleeve 3 is 0.5mm, and length is 25mm.Under this size, this device can reach a best working effect.Adsorption magnet 1 is arranged in overcoat 2 and inner sleeve 3, and can move up and down in overcoat 2 and inner sleeve 3, and inner sleeve 3 is connected by pressure protect key 4 with overcoat 2.Top non-magnetic material in overcoat 2 installs the lid 5 that binds.
With reference to Fig. 1, during the state of do not work when this device is in (non-adsorbed), adsorption magnet 1 is adsorbed in overcoat 2, and shell is effectively protected adsorption magnet 1, prevents adsorption magnet 1 from being polluted.With reference to Fig. 2, when this device is in Job readiness (absorption), by manual operation or instrument controlling, adsorption magnet 1 is taken off from fixed magnet 5, the fore portion of adsorption magnet 1 exceedes the opening of inner sleeve 3, adsorption magnet 1 position in inner sleeve 3, be in and can adsorb magnetic bead and binding substances situation thereof, such preparation work is complete.With reference to Fig. 3, when this device starts to adsorb work, plastic jacket 6 is sleeved on the lower outside of inner sleeve 3, plastic jacket 6 protects adsorption magnet 1 directly not adsorb with magnetic bead and binding substances thereof, and magnetic bead 7, under the effect of adsorption magnet 1, is attracted on the surface of plastic jacket 6.With reference to Fig. 4, when this device is in release magnetic bead and binding substances thereof, automatically controlled by artificial or instrument, under the action of a magnetic field of the two like magnetic poles repel each other bottom sample, adsorption magnet 1 is pushed up from inner sleeve 3, to the top of magnet cover, magnetic bead 7 is not having adsoption-magnetic field, and under having the effect of release magnetic field (bottom of sample container is equipped with the magnet of two like magnetic poles repel each other), be discharged in suitable container.With reference to Fig. 5, exceed to a certain degree when this device is subject to downward pressure, but time immotile; pressure protect key 4 loosens, and the adsorption magnet 1 in inner sleeve 3 is pushed in overcoat 2, now; only have plastic jacket 6 impaired, but adsorption magnet 1 structure and biological specimen can be protected.This situation, when forgetting the lid taking sample culture dish away, very useful.
Embodiment 2
As different from Example 1, adsorption magnet 1 is after completing release magnetic bead and binding substances thereof, and move to new local time, adsorption magnet 1 can come back to again inner sleeve 3 place, is adsorption operations next time, ready for the present embodiment.Just, adsorption magnet be can have been come by artificial or instrument controlling up and down, can by placement external magnet at corresponding Tissue Culture Dish trench bottom, or the top of inner magnet, utilize the two like magnetic poles repel each other in magnetic field, the principle that there is a natural attraction between the sexes, reach the object controlling adsorption magnet and move up and down.
This apparatus structure is simple, production cost is low, this device can under instrument or artificial control, the magnetic field of conversion absorption and release magnetic bead and binding substances thereof effectively, reach the object of collecting magnetic bead and binding substances thereof, easy to operate, loaded down with trivial details before decreasing, by the insertion plastic jacket of permanent magnet with the operation steps such as to extract in plastic jacket out, reach the object of absorption and release magnetic bead and binding substances thereof; This device has protection biological specimen and magnetic head itself is without prejudice simultaneously, for device gives a warning condition, be convenient to check in time correlated condition in biological sample analysis process and operation whether correct.
It should be noted last that, above embodiment is only in order to illustrate the technical solution of the utility model and unrestricted, although be described in detail the utility model with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can modify to the technical solution of the utility model or equivalent replacement, and not departing from the spirit and scope of technical solutions of the utility model, it all should be encompassed in the middle of right of the present utility model.
Claims (10)
1. a magnetic bead and the separatory magnetic head of binding substances thereof, it is characterized in that, comprise shell and adsorption magnet, described shell is made up of jacket and inner sleeve, described inner sleeve can move up and down in described overcoat, described adsorption magnet is arranged in described overcoat and described inner sleeve, and can move up and down in described overcoat and described inner sleeve.
2. magnetic bead according to claim 1 and the separatory magnetic head of binding substances thereof, is characterized in that, described binding substances refers to the biomass cells and biomacromolecule that combine with magnetic bead.
3. magnetic bead according to claim 1 and the separatory magnetic head of binding substances thereof, is characterized in that, the diameter of described inner sleeve is less than the diameter of described overcoat.
4. magnetic bead according to claim 2 and the separatory magnetic head of binding substances thereof, it is characterized in that, described inner sleeve is connected by pressure protect key with described overcoat.
5. magnetic bead according to claim 2 and the separatory magnetic head of binding substances thereof, is characterized in that, be provided with permanent magnet in described inner sleeve.
6. magnetic bead according to claim 1 and the separatory magnetic head of binding substances thereof, is characterized in that, the bottom of described inner sleeve is opening form or closed.
7. magnetic bead according to claim 1 and the separatory magnetic head of binding substances thereof, is characterized in that, the subsidiary material of described shell and inner casing are non-magnetic material.
8. magnetic bead according to claim 1 and the separatory magnetic head of binding substances thereof, it is characterized in that, the bottom of described inner sleeve is set with plastic jacket, and described plastic jacket is directly adsorbed onto magnet for avoiding magnetic bead and binding substances thereof.
9. magnetic bead according to claim 1 and the separatory magnetic head of binding substances thereof, is characterized in that, moving up and down of described adsorption magnet can be reached by the effect of external magnetic field.
10. magnetic bead according to claim 9 and the separatory magnetic head of binding substances thereof, it is characterized in that, adsorption magnet moves, and extraneous magnetic field is by different pole orientation, top or the bottom of magnetic head can be added in, act on inner adsorption magnet and make it to reach and move up and down.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420382458.3U CN204174197U (en) | 2014-07-10 | 2014-07-10 | A kind of magnetic bead and the separatory magnetic head of binding substances thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420382458.3U CN204174197U (en) | 2014-07-10 | 2014-07-10 | A kind of magnetic bead and the separatory magnetic head of binding substances thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204174197U true CN204174197U (en) | 2015-02-25 |
Family
ID=52563022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420382458.3U Expired - Lifetime CN204174197U (en) | 2014-07-10 | 2014-07-10 | A kind of magnetic bead and the separatory magnetic head of binding substances thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204174197U (en) |
-
2014
- 2014-07-10 CN CN201420382458.3U patent/CN204174197U/en not_active Expired - Lifetime
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20150225 |