CN204090602U - Matsutake mycelia culture tank - Google Patents
Matsutake mycelia culture tank Download PDFInfo
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- CN204090602U CN204090602U CN201420275415.5U CN201420275415U CN204090602U CN 204090602 U CN204090602 U CN 204090602U CN 201420275415 U CN201420275415 U CN 201420275415U CN 204090602 U CN204090602 U CN 204090602U
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- tank body
- tank
- air intake
- matsutake
- culture tank
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- 241000121220 Tricholoma matsutake Species 0.000 title claims abstract description 47
- 238000011081 inoculation Methods 0.000 claims abstract description 15
- 238000003756 stirring Methods 0.000 claims abstract description 15
- 238000011534 incubation Methods 0.000 abstract description 4
- 230000001737 promoting effect Effects 0.000 abstract description 4
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 230000002503 metabolic effect Effects 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 19
- 239000012531 culture fluid Substances 0.000 description 15
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 13
- 239000001301 oxygen Substances 0.000 description 13
- 229910052760 oxygen Inorganic materials 0.000 description 13
- 238000000034 method Methods 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 5
- 230000000903 blocking effect Effects 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000008204 material by function Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- VQKFNUFAXTZWDK-UHFFFAOYSA-N alpha-methylfuran Natural products CC1=CC=CO1 VQKFNUFAXTZWDK-UHFFFAOYSA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000505 pernicious effect Effects 0.000 description 1
- 238000010223 real-time analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of matsutake mycelia culture tank, belong to the artificial cultivation field of matsutake mycelia.Described culture tank comprises: tank body (1), cover (2), stirring rod (3), paddle (4), gas outlet (5), inoculation mouth (6), two outer loop interfaces (7) and (8), sample tap (9), air intake (11); Wherein the top of tank body (1) and middle part are ball-type, and bottom is gentle slope type tank neck, and whole tank body (1) is in balloon shape.The special culture tank of the utility model matsutake mycelia solves matsutake mycelia incubation growth slowly and be difficult to the problems such as a large amount of productions, there is volume little, cost is low, energy consumption is low, easy and simple to handle, efficiency advantages of higher, has important application potential in the deep exploitation etc. of matsutake field artificial propagation promoting and functional metabolic product.
Description
Technical field
The utility model relates to the cultivation special purpose device of a kind of fungi, particularly relates to the culture apparatus of matsutake mycelia, belongs to the artificial cultivation field of matsutake mycelia.
background technology
Matsutake is precious sylvan life wild fungi, has formally been listed in Chinese Second Class Key Protected Plant register.Matsutake is a kind of ectotrophic mycorrhiza forming trophobiosis relation with pine tree, and the root system of live body trees must be relied on to maintain growth; Because matsutake is very harsh to the requirement of growth conditions, at present still can not artificial cultivation.Due to destruction and the unordered pernicious collection of forest ecological environment, the biosphere of its growth reduces increasingly, and resource is day by day deficient; Thus the demand of matsutake artificial propagation promoting and functional materials deep exploitation is more and more large, and its basis is exactly a large amount of rapid culture techniques of matsutake mycelia.
At present; the incubator that the cultivation of matsutake mycelia adopts is triangular flask or cylindrical shape; all exist and be separated difficulty, poor growth, small scale and the problem such as yield is low; as 500ml triangular flask liquid culture yield is only about 4g/L, although have its cultivation amount of the report of yield about 20g/L to be only 100ml scale or incubation time reaches more than 1 month.In the artificial cultivation of matsutake mycelia the carrying out of problem seriously the constrains matsutake field artificial propagation promoting technology such as ubiquitous small scale, cycle long and yield is low and functional materials deep development research on utilization, urgently improve.
utility model content
The purpose of this utility model is to provide a kind ofly cultivates the matsutake mycelia culture apparatus special that scale is large, the cycle is short, the yield of matsutake mycelia is high.
Above-mentioned purpose of the present utility model is achieved through the following technical solutions:
A special culture tank for matsutake mycelia, comprising: tank body, cover, stirring rod, paddle, gas outlet, inoculation mouth (or material-feeding port), sample tap, air intake; Wherein, cover, gas outlet and inoculation mouth (or material-feeding port) are arranged on the upper end of tank body; Stirring rod and paddle are positioned at the lumen centers of tank body; Sample tap is arranged on the lower end of tank body; In sample tap, be provided with control valve, in air intake, be provided with control valve; Wherein, the top of tank body and the volume at middle part are significantly greater than the volume of tank body lower part and volume in the middle part of tank body is maximum.
In order to reach best technique effect, preferably, the top of tank body and middle part are ball-type, and the bottom of tank body is gentle slope type tank neck, and whole tank body is balloon shape.
The culture tank of current matsutake mycelia is triangular flask or cylindrical shape, cultivates matsutake mycelia by the culture tank of triangular flask or cylindrical shape, all there is the problems such as yield is low, cultivation small scale, cultivation cycle are long; The utility model is found by a large amount of experiments, in employing, upper volume is large, the air contact surfaces that the balloon shape culture tank that bottom volume gentle slope type reduces effectively can increase unit volume culture fluid amasss, improve the molten amount of oxygen of culture fluid, what minimizing bubbles burst brought to cell coerces, strengthen the entirety circulation of culture fluid, impel cultural hypha even, finally significantly improve the culture efficiency of matsutake mycelia.Confirm through test, compare the cultivation tank body of other shapes such as existing cylindrical or triangle, gas spherical tank can significantly improve the culture efficiency of matsutake mycelia.
The utility model finds further, in incubation, the method of supplying that oxygen supply adopts bubble type to enter gas (from culture tank bottom gas-feeding) can guarantee the sufficient supplies of oxygen and being evenly distributed of oxygen, effectively can improve the culture efficiency of matsutake mycelia; Therefore, air intake is preferably arranged on the bottommost of culture tank by the utility model, realizes from culture tank bottom gas-feeding oxygen.In addition, an air intake control valve is set in air intake, can guarantees to cultivate (air feed) front and cultivate after (air feed) terminate, to avoid in tank body culture fluid water clock and the situation such as contaminated.
Bottom culture tank during supply oxygen, the mycelia apposition growth near air intake and agglomerating often can cause not smooth into gas passage or blocking, finally cause cultural hypha inefficiency; In order to solve the problem, the utility model arranges two outer loop interfaces being abutted against above air intake, two outer loop interface flexible pipes are connected, the fluid circulation of culture fluid is formed near air intake, prevent mycelia apposition growth near air intake and the agglomerating caused blocking entering gas passage, ensure to cultivate steadily and high efficiency.
The sample tap being arranged on culture tank tank body lower part is convenient to nutrient source, pH value and the metabolite composition transfer such as real-time analysis carbon nitrogen; Wherein, the control valve be arranged in sample tap can ensure water clock and the pollution of culture fluid in non-sampling period tank body.
The gas outlet being arranged on culture tank tank body top can ensure constant voltage and the reduction of the culture efficiency avoiding abnormal tank pressure to bring.
The stirring rod and the paddle that are arranged in the inner chamber of culture tank tank body can prevent bubble generation, harmful components are formed and the reduction of agglomerating the brought culture efficiency of mycelia.
The special culture tank of matsutake mycelia of the present utility model, solve matsutake mycelia incubation growth and slowly and in a large number produce the problems such as difficult, there is volume little, cost is low, energy consumption is low, easy and simple to handle, efficiency advantages of higher, the utility model has important application potential in the deep exploitation etc. of matsutake field artificial propagation promoting and functional metabolic product.
Accompanying drawing explanation
Fig. 1 is a kind of structural representation of the special culture tank of the utility model matsutake mycelia.
Fig. 2 is another structural representation of the special culture tank of the utility model matsutake mycelia.
Reference numeral: 1-tank body, 2-cover, 3-stirring rod, 4-paddle, 5-gas outlet, 6-inoculates mouth (or material-feeding port), and 7-is away from the outer loop interface of air intake, and 8-is near the outer loop interface of air intake, 9-sample tap, 10-is arranged on the control valve in sample tap, 11-air intake, and 12-is arranged on the control valve in air intake.
Embodiment
Further describe the utility model below in conjunction with specific embodiment, advantage and disadvantage of the present utility model will be more clear along with description.But these embodiments are only exemplary, do not form any restriction to scope of the present utility model.It will be understood by those skilled in the art that and do not departing under spirit and scope of the present utility model and can modify to details of the present utility model and form or replace, but these amendments and replacement all fall in protection domain of the present utility model.
Below in conjunction with Figure of description, structure of the present utility model and technique effect are described further.
Fig. 1 is the first structural representation of the special culture tank of matsutake mycelia of the present utility model; It comprises: tank body (1), cover (2), stirring rod (3), paddle (4), gas outlet (5), inoculation mouth or material-feeding port (6), sample tap (9), air intake (11); Wherein, cover (2), gas outlet (5) and inoculation mouth or material-feeding port (6) are arranged on the upper end of tank body (1); Stirring rod (3) and paddle (4) are positioned at the lumen centers of tank body (1); Sample tap (9) is arranged on the lower end of tank body (1); Control valve (10) is provided with in sample tap (9); Air intake (11) is arranged on the bottommost of culture tank, is provided with control valve (12) in air intake (11); Oxygen supply can guarantee the sufficient supplies of oxygen and being evenly distributed of oxygen from the method for supplying bottom culture tank, effectively can improve the culture efficiency of matsutake mycelia; In addition, an air intake control valve is set in air intake, can guarantees to cultivate (air feed) front and cultivate after (air feed) terminate, to avoid in tank body culture fluid water clock and the situation such as contaminated.
Top and the middle part of tank body (1) are ball-type, and the bottom of tank body is gentle slope type tank neck, and whole tank body is balloon shape.In employing, upper volume is large, the air contact surfaces that the balloon shape culture tank that bottom volume gentle slope type reduces effectively can increase unit volume culture fluid amasss, improve the molten amount of oxygen of culture fluid, what minimizing bubbles burst brought to cell coerces, strengthen the entirety circulation of culture fluid, impel cultural hypha even, finally significantly improve the culture efficiency of matsutake mycelia.Confirm through test, compare the cultivation tank body of other shapes such as existing cylindrical or triangle, gas spherical tank can significantly improve the culture efficiency of matsutake mycelia.
The using method of the culture tank of this matsutake mycelia comprises: be injected into by the medium of matsutake mycelia in tank body (1), and the volume of medium is no more than 80% of tank body (1) volume; Matsutake bacterial classification is inoculated in the medium of tank body (1) by inoculation mouth or material-feeding port (6), opens stirring rod (3), carry out the cultivation of matsutake mycelia; Open the control valve (12) arranged in air intake (11), by the bottom of tank body to medium supply oxygen; In good time unlatching gas outlet (5), gets rid of the too much gas in tank body, ensures the constant voltage in tank body; In culture tank, sample analysis is taken out by sample tap (9); Supplemented the nutrients in tank body by inoculation mouth (or material-feeding port) (6) in good time.
Fig. 2 is the another kind of structural representation of the special culture tank of the utility model matsutake mycelia; It comprises: tank body (1), cover (2), stirring rod (3), paddle (4), gas outlet (5), inoculation mouth (or material-feeding port) (6), two outer loop interfaces (7) and (8), sample tap (9), air intake (11); Wherein, cover (2), gas outlet (5) and inoculation mouth (or material-feeding port) (6) are arranged on the upper end of tank body (1); Stirring rod (3) and paddle (4) are positioned at the lumen centers of tank body (1); Sample tap (9) is arranged on the lower end of tank body (1); Control valve (10) is provided with in sample tap (9); Air intake (11) is arranged on the bottommost of culture tank, is provided with control valve (12) in air intake (11); Two outer loop interfaces (7) and (8) are arranged on the top of being abutted against air intake; Two outer loop interface flexible pipes are connected, and form the fluid circulation of culture fluid near air intake, prevent mycelia apposition growth near air intake and the agglomerating caused blocking entering gas passage, ensure to cultivate steadily and high efficiency.
Top and the middle part of tank body (1) are ball-type, and the bottom of tank body is gentle slope type tank neck, and whole tank body is balloon shape.In employing, upper volume is large, the air contact surfaces that the balloon shape culture tank that bottom volume gentle slope type reduces effectively can increase unit volume culture fluid amasss, improve the molten amount of oxygen of culture fluid, what minimizing bubbles burst brought to cell coerces, strengthen the entirety circulation of culture fluid, impel cultural hypha even, finally significantly improve the culture efficiency of matsutake mycelia.Confirm through test, compare the cultivation tank body of other shapes such as existing cylindrical or triangle, gas spherical tank can significantly improve the culture efficiency of matsutake mycelia.
The using method of the culture tank of this matsutake mycelia comprises: be injected into by the liquid nutrient medium of matsutake mycelia in tank body (1), and the volume of liquid nutrient medium is no more than 80% of tank body (1) volume; Matsutake bacterial classification is inoculated in the medium of tank body (1) by inoculation mouth (or material-feeding port) (6), opens stirring rod (3), carry out the cultivation of matsutake mycelia; Open the control valve (12) arranged in air intake (11), by the bottom of tank body to medium supply oxygen; Two outer loop interfaces (7) are connected with flexible pipe with (8), near air intake, form the fluid circulation of culture fluid; In good time unlatching gas outlet (5), gets rid of the too much gas in tank body, ensures the constant voltage in tank body; In culture tank, sample analysis is taken out by sample tap (9); Supplemented the nutrients in tank body by inoculation mouth (or material-feeding port) (6) in good time.
Claims (8)
1. a special culture tank for matsutake mycelia, comprising: tank body (1), cover (2), stirring rod (3), paddle (4), gas outlet (5), inoculation mouth (6), sample tap (9), air intake (11); Wherein, cover (2), gas outlet (5) and inoculation mouth (6) are arranged on the upper end of tank body (1); Stirring rod (3) and paddle (4) are positioned at the lumen centers of tank body (1); Sample tap (9) is arranged on the lower end of tank body (1); In sample tap (9), be provided with control valve (10), in air intake (11), be provided with control valve (12); It is characterized in that: the top of tank body (1) and the volume at middle part are significantly greater than the volume of tank body lower part and volume in the middle part of tank body is maximum.
2. according to special culture tank according to claim 1, it is characterized in that: top and the middle part of tank body (1) are ball-type, the bottom of tank body (1) is gentle slope type tank neck.
3. according to the special culture tank described in claim 1 or 2, it is characterized in that: the entirety of tank body (1) is balloon shape.
4. according to special culture tank according to claim 1, it is characterized in that: bottommost air intake (11) being arranged on culture tank; An air intake control valve (12) is set in air intake (11).
5. a special culture tank for matsutake mycelia, comprising: tank body (1), cover (2), stirring rod (3), paddle (4), gas outlet (5), inoculation mouth (6), sample tap (9), air intake (11); Wherein, cover (2), gas outlet (5) and inoculation mouth (6) are arranged on the upper end of tank body (1); Stirring rod (3) and paddle (4) are positioned at the lumen centers of tank body (1); Sample tap (9) is arranged on the lower end of tank body (1); In sample tap (9), be provided with control valve (10), in air intake (11), be provided with control valve (12); It is characterized in that: the top of tank body (1) and the volume at middle part are significantly greater than the volume of tank body lower part and volume in the middle part of tank body is maximum; Two outer loop interfaces (7) and (8) are set in the top of being abutted against air intake (11); Outer loop interface (7) is away from air intake (11), and outer loop interface (8) is near air intake (11).
6. according to special culture tank according to claim 5, it is characterized in that: top and the middle part of tank body (1) are ball-type, the bottom of tank body (1) is gentle slope type tank neck.
7. according to the special culture tank described in claim 5 or 6, it is characterized in that: the entirety of tank body (1) is balloon shape.
8. according to special culture tank according to claim 5, it is characterized in that: bottommost air intake (11) being arranged on culture tank; An air intake control valve (12) is set in air intake (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420275415.5U CN204090602U (en) | 2014-05-27 | 2014-05-27 | Matsutake mycelia culture tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420275415.5U CN204090602U (en) | 2014-05-27 | 2014-05-27 | Matsutake mycelia culture tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204090602U true CN204090602U (en) | 2015-01-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420275415.5U Expired - Fee Related CN204090602U (en) | 2014-05-27 | 2014-05-27 | Matsutake mycelia culture tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204090602U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104025904A (en) * | 2014-05-27 | 2014-09-10 | 中国林业科学研究院森林生态环境与保护研究所 | Culturing method for tricholoma matsutake mycelia and special device thereof |
-
2014
- 2014-05-27 CN CN201420275415.5U patent/CN204090602U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104025904A (en) * | 2014-05-27 | 2014-09-10 | 中国林业科学研究院森林生态环境与保护研究所 | Culturing method for tricholoma matsutake mycelia and special device thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150114 |