CN203923196U - Test kit - Google Patents

Test kit Download PDF

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Publication number
CN203923196U
CN203923196U CN201420296925.0U CN201420296925U CN203923196U CN 203923196 U CN203923196 U CN 203923196U CN 201420296925 U CN201420296925 U CN 201420296925U CN 203923196 U CN203923196 U CN 203923196U
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CN
China
Prior art keywords
box body
test kit
thalline
centrifuge tube
check
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201420296925.0U
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Chinese (zh)
Inventor
吕利群
李梦影
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Shanghai Maritime University
Shanghai Ocean University
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Shanghai Maritime University
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Priority to CN201420296925.0U priority Critical patent/CN203923196U/en
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Publication of CN203923196U publication Critical patent/CN203923196U/en
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Abstract

The utility model relates to detection instrument field, particularly a kind of test kit, and it detects for aquaculture thalline susceptibility, can realize quickly and easily thalline susceptibility and detect, and comprising: box body; Check-out console, it is placed on box body, and has 12 circle detection shrinkage pools with 3 × 4 array arrangements, applies the fungus resistant coating that contains dry nystatin on the inwall of each described detection shrinkage pool; Centrifuge tube, it is placed in the groove of box body, and comprises the pipe lid of elongated body and sealed tube body; Thalline sampling instrument, it is placed in the cavity of box body; Lid, it is pivotally connected to box body, and when in closing condition and box body jointly by check-out console, centrifuge tube and the sealing of thalline sampling instrument.

Description

Test kit
Technical field
The utility model relates to detection instrument field, particularly a kind of test kit.
Background technology
In recent years, bacteriosis becomes the most serious class transmissible disease of harm cultured freshwater fish, but due to antibiotic abuse, makes pathogenic bacterium very fast to antibiotic Susceptible change; In addition, life-time service microbiotic makes pathogenic bacterium occur resistance problem, only with sometimes poor effect of experience medication in the past.Therefore, when disease occurs, first need to carry out drug screening and Drug level and judge, and suit the remedy to the case on this basis, to control as early as possible disease, reduce fisherman's loss.
At present mainly adopt K-B paper disk method for the detection method of aquaculture bacteriosis, related products is mainly the susceptibility detection kit forming by posting antibiotic drug sensitive test paper and culture dish.This product, due to substratum meeting breed bacteria, fungi in preservation process, thereby long-term preservation and the use of uncomfortable aquaculture.Detect by this method and need to take a long time, and can only carry out preliminary drug screening, all can not effectively measure aquatic products Drug level; In addition, adopt these methods to need professional knowledge and Experiment on Microbiology condition to carry out susceptibility test experience, and common aquaculturist and fishery technology department of basic unit do not possess professional knowledge conventionally and Experiment on Microbiology condition is carried out susceptibility test experience, thereby be difficult to carry out quickly and easily susceptibility test experience at aquaculture one line scene and some fishery technology departments of basic unit.
Utility model content
Embodiment of the present utility model provides a kind of test kit, detects for aquaculture thalline susceptibility, can realize quickly and easily thalline susceptibility and detect.
According to embodiment of the present utility model, a kind of test kit is provided, detect for aquaculture thalline susceptibility, comprising:
Box body;
Check-out console, it is placed on box body, and has 12 circle detection shrinkage pools with 3 × 4 array arrangements;
Centrifuge tube, it is placed in the groove of box body, and comprises the pipe lid of elongated body and sealed tube body, applies the fungus resistant coating that contains dry nystatin on the inwall of each described detection shrinkage pool;
Thalline sampling instrument, it is placed in the cavity of box body;
Lid, it is pivotally connected to box body, and when in closing condition and box body jointly by check-out console, centrifuge tube and the sealing of thalline sampling instrument.
Preferably, in each embodiment of the present utility model, detect that shrinkage pool is of similar shape and size described in each.
Preferably, in each embodiment of the present utility model, on the inwall of each described detection shrinkage pool, comprise: the coating that comprises dry nystatin.
Preferably, in each embodiment of the present utility model, described thalline sampling instrument comprises: one or more in cotton swab, tweezers, scissors.
Preferably, in each embodiment of the present utility model, described test kit further comprises:
Timer, it is arranged on described box body;
And/or
Temperature sensor, it is arranged on described box body.
Preferably, in each embodiment of the present utility model, described test kit further comprises:
Electric heating and thermal insulation device, it is arranged on described box body.
Preferably, in each embodiment of the present utility model, described electric heating and thermal insulation device comprises: the one or more electric heating bars that extend along the periphery of described check-out console.
Preferably, in each embodiment of the present utility model, described test kit further comprises:
Shading valve, it is installed to the described lid with transparent region slidably, blocks described transparent region and when in open site, expose described transparent region when time in the closed position.
Preferably, in each embodiment of the present utility model, described check-out console is installed to described box body by a rotating shaft, and described check-out console can make to detect shrinkage pool from transferring to upward downward around described rotating shaft rotation.
Preferably, in each embodiment of the present utility model, described test kit further comprises:
Centrifuge tube bracing frame, it clamps described centrifuge tube, and when in holding state, described centrifuge tube is remained on to vertical position.
The test kit providing by embodiment of the present utility model can be realized quickly and easily thalline susceptibility and detect in the time carrying out the detection of aquaculture thalline susceptibility.
Brief description of the drawings
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be discussed below, obviously, only embodiment more of the present utility model in the technical scheme being described by reference to the accompanying drawings, for those of ordinary skills, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawing illustrated embodiments other embodiment and accompanying drawing thereof.
Fig. 1 is according to the structural representation of the test kit of embodiment of the present utility model.
Embodiment
Below with reference to accompanying drawing, the technical scheme of the each embodiment of the utility model is known to complete description, obviously, described embodiment is only a part of embodiment of the present utility model, instead of whole embodiment.Based on the embodiment described in the utility model, all other embodiment that those of ordinary skill in the art obtain under the prerequisite that does not need creative work, in the scope of protecting at the utility model.
Embodiment of the present utility model provides a kind of test kit, detects for aquaculture thalline susceptibility, can realize quickly and easily thalline susceptibility and detect.
According to embodiment of the present utility model, a kind of test kit is provided, detect for aquaculture thalline susceptibility, comprising:
Box body;
Check-out console, it is placed on box body, and has 12 circle detection shrinkage pools with 3 × 4 array arrangements, applies the fungus resistant coating that contains dry nystatin on the inwall of each described detection shrinkage pool;
Centrifuge tube, it is placed in the groove of box body, and comprises the pipe lid of elongated body and sealed tube body;
Thalline sampling instrument, it is placed in the cavity of box body;
Lid, it is pivotally connected to box body, and when in closing condition and box body jointly by check-out console, centrifuge tube and the sealing of thalline sampling instrument.
Like this, in described test kit, be provided with the assembly of the multiple necessity that detect for aquaculture thalline susceptibility, comprise: for carrying check-out console (multiple detection shrinkage pools being set to hold respectively target thalline and different medicament samples to be measured on it), the centrifuge tube for admixing medical solutions, the thalline sampling instrument of target thalline and medicament to be measured, these assemblies all dressing also can seal by lid in the box body of test kit.Especially, on the inwall of each described detection shrinkage pool, apply the fungus resistant coating that contains dry nystatin, like this, by comprising the coating of nystatin, on the one hand can bacteria growing inhibiting and thalline susceptibility is detected and cause interference, the effectively growth of Antifungi on the other hand, to avoid available reagent box incident mildew phenomenon in the time preserving thalline for a long time.By this test kit of the present utility model, can carry out aquaculture thalline (Aeromonas veronii that Aeromonas hydrophila, grass carp or the black carp that such as bighead or crucian infect infects etc.) susceptibility in mode conveniently and detect, particularly more convenient for aquaculture one line and some fishery technology departments of basic unit.
Thus, the described test kit that the utility model provides, can be applied to the screening of the quick types of medicines of the on-the-spot bacteriosis of aquaculture and the judgement of Drug level, realizes medication quickly and easily thus detect for concrete bacterium thalline.
Preferably, in each embodiment of the present utility model, detect that shrinkage pool is of similar shape and size described in each.Like this, carry out when thalline susceptibility detects making these medicaments remain in identical testing environment at the different medicaments that difference detected in shrinkage pool, be easy to detect and comparison result.
In actual applications, can the antibacterial effect of different pharmaceutical (as antibiotic medicine) be compared or the antibacterial effect under the different concns of same medicine is compared, the medicines such as such as doxycycline, norfloxicin, Enrofloxacin, florfenicol, trimethoprim-sulfamethoxazole, gentamicin.
The solid medium that can comprise the medicine (for example microbiotic) of different sorts, concentration in each described detection shrinkage pool, carries out drug effect contrast and drug screening thus.
Preferably, in each embodiment of the present utility model, there is cumulative groove depth for the described groove of settling described centrifuge tube.Like this, centrifuge tube is in the time being placed in groove, and its mouth of pipe can pollute to prevent the nutrient solution outflow in centrifuge tube a little more than the pipe end.
Preferably, in each embodiment of the present utility model, described thalline sampling instrument comprises: one or more in cotton swab, tweezers, scissors.Should be understood that as required, thalline sampling instrument also can comprise other utensil, such as pipette etc.
Preferably, in each embodiment of the present utility model, described test kit further comprises:
Timer, it is arranged on described box body.
Like this, in the time different medicaments being carried out to thalline susceptibility detection (sieve medicine), can set the different concern time, be beneficial to carry out corresponding detecting step.For example, during with centrifuge culture thalline, can be 8-12 hour by yeast culture set of time, in the time arriving setting-up time, can remind (for example sending audio or video signal by audio or video prompting device reminds) user to carry out corresponding operating, for example observation detects thalli growth situation in shrinkage pool etc.
Preferably, in each embodiment of the present utility model, described test kit further comprises:
Temperature sensor, it is arranged on described box body.
Like this, in the time different medicaments being carried out to thalline susceptibility detection (sieve medicine) for concrete thalline, can set applicable envrionment temperature, the growth that is detected thalline to be adapted to is temperature required, is beneficial to carry out corresponding detecting step.For example, the centrifuge tube of cultivating thalline is placed at 20-30 DEG C and is cultivated.。
In one embodiment, described test kit can comprise that timer and temperature sensor are used in conjunction with simultaneously, and the quantity of timer and temperature sensor also can be determined according to specific needs.
Preferably, in each embodiment of the present utility model, described test kit further comprises:
Electric heating and thermal insulation device, it is arranged on described box body.
Like this, in the time different medicaments being carried out to the detection of thalline susceptibility for concrete thalline, can set applicable envrionment temperature, thereby detected thalline is grown in applicable temperature, be beneficial to carry out follow-up corresponding detecting step.For example, the centrifuge tube of having cultivated thalline is left standstill and cultivated, can leave standstill 8~12 hours at 25~30 DEG C, or can leave standstill 12~18 hours at 20~25 DEG C.
Preferably, in each embodiment of the present utility model, described electric heating and thermal insulation device comprises: the one or more electric heating bars that extend along the periphery of described check-out console.Like this, in electric heating bar is enclosed in the multiple detection shrinkage pools on check-out console along check-out console circumferential distribution, limit the thalline that a metastable space of temperature is beneficial to cultivate in these multiple detection shrinkage pools grows under metastable environment, make thus detected result more reliable, this around in the relatively low situation of envrionment temperature (for example, in winter) advantageous particularly.
Preferably, in each embodiment of the present utility model, described test kit further comprises:
Shading valve, it is installed to the described lid with transparent region slidably, blocks described transparent region and when in open site, expose described transparent region when time in the closed position.
Like this, shading valve can expose the transparent region of lid when in open site, testing staff can be from the situation in transparent region box of inspection, for example detect the growing state of thalline in shrinkage pool, and will whole not the opening of lid and may have influence on the growth (such as temperature fluctuation, pollution microbes etc.) of thalline; Shading valve shelters from this transparent region and makes to remain in shading environment to adapt to thalli growth needs in box when time in the closed position.
Preferably, in each embodiment of the present utility model, described check-out console is installed to described box body by a rotating shaft, and described check-out console can make to detect shrinkage pool from transferring to upward downward around described rotating shaft rotation.Like this, thalline to be detected is applied to check-out console upward (, towards box opening or lid) each detection shrinkage pool in after (and can further wait until thalline to be detected dry after), check-out console rotation can be made to detect shrinkage pool transfers downward (, towards box body inside or bottom surface) to and makes to detect in the lucifuge environment of thalline to be detected in shrinkage pool relative closure in box body and grow.In favourable embodiment, described rotating shaft can be along the center line setting of check-out console, makes still can form with other parts of box body the structure of relative closure after check-out console upset downwards; But implement, in side, also can described rotating shaft be set along of a check-out console edge at other, make check-out console entirety to side-overturning.
Preferably, in each embodiment of the present utility model, described test kit further comprises:
Centrifuge tube bracing frame, it clamps described centrifuge tube, and when in holding state, described centrifuge tube is remained on to vertical position.
Like this, can avoid centrifuge tube to tilt and nutrient solution leakage is wherein polluted.
Visible, in described testing cassete, can be provided for aquaculture bacteriosis and sieve fast the assembly of multiple necessity of medicine, comprise: add the solid medium of different medicaments and the check-out console (multiple detection shrinkage pools being set to hold respectively different sorts, concentration medicament and target thalline on it) of carrying target thalline, for mixing centrifuge tube, the thalline sampling instrument of thalline, these assemblies all dressing in the box body of test kit and can seal by lid.Especially, can comprise the microbiotic of different sorts, concentration in each described detection shrinkage pool, high density is wherein anti-sudden change Drug level, has solved so the long-term non-rational use of drug of current aquaculture, causes pathogenic bacteria to occur resistance problem.Especially, in each described detection shrinkage pool, comprise can Antifungi growth but nystatin to bacterium unrestraint effect, to avoid available reagent box incident mildew phenomenon when long-time the preservation.By this test kit of the present utility model, can such as, sieve fast medicine for aquaculture bacterium (Aeromonas veronii that Aeromonas hydrophila, grass carp or the black carp that bighead or crucian infect infects etc.) in mode conveniently, particularly more convenient for aquaculture one line and some fishery technology departments of basic unit.
Fig. 1 is according to the structural representation of the test kit of embodiment of the present utility model, that is, and and vertical view, and removed lid to demonstrate the internal structure of test kit for clear demonstration.Visible a kind of test kit in the embodiment shown in fig. 1, detects for aquaculture thalline susceptibility, comprising:
Box body;
Check-out console 100, it is placed on box body, and has 12 circle detection shrinkage pools 101 with 3 × 4 array arrangements, applies the fungus resistant coating that contains dry nystatin on the inwall of each described detection shrinkage pool 101;
Centrifuge tube (not shown), it is placed in the groove 200 of box body, and comprises the pipe lid of elongated body and sealed tube body;
Thalline sampling instrument (not shown), it is placed in the cavity 300 of box body;
Lid (be removed in the drawings and not shown), it is pivotally connected to box body, and when in closing condition and box body jointly by check-out console 100, centrifuge tube and the sealing of thalline sampling instrument.
The test kit providing by embodiment of the present utility model, detect carrying out can realizing quickly and easily thalline susceptibility when aquaculture thalline susceptibility detects, and can carry out rapid detection and active drug screens to select applicable medication to aquaculture bacteriosis thus.
The various embodiment that the utility model provides combination mutually in any way as required, the technical scheme obtaining by this combination, also in scope of the present utility model.
Obviously,, in the situation that not departing from spirit and scope of the present utility model, those skilled in the art can carry out various changes and modification to the utility model.If be within the scope of claim of the present utility model and equivalent thereof to these changes of the present utility model and modification, the utility model also will comprise these changes and modification.

Claims (10)

1. a test kit, detects for aquaculture thalline susceptibility, it is characterized in that, comprising:
Box body;
Check-out console, it is placed on box body, and has 12 circle detection shrinkage pools with 3 × 4 array arrangements, applies the fungus resistant coating that contains dry nystatin on the inwall of each described detection shrinkage pool;
Centrifuge tube, it is placed in the groove of box body, and comprises the pipe lid of elongated body and sealed tube body;
Thalline sampling instrument, it is placed in the cavity of box body;
Lid, it is pivotally connected to box body, and when in closing condition and box body jointly by check-out console, centrifuge tube and the sealing of thalline sampling instrument.
2. test kit as claimed in claim 1, is characterized in that,
Described in each, detect that shrinkage pool is of similar shape and size.
3. test kit as claimed in claim 1, is characterized in that,
For settling the described groove of described centrifuge tube to there is cumulative groove depth.
4. test kit as claimed in claim 1, is characterized in that,
Described thalline sampling instrument comprises: one or more in cotton swab, tweezers, scissors.
5. test kit as claimed in claim 1, is characterized in that, further comprises:
Timer, it is arranged on described box body;
And/or
Temperature sensor, it is arranged on described box body.
6. test kit as claimed in claim 1, is characterized in that, further comprises:
Electric heating and thermal insulation device, it is arranged on described box body.
7. test kit as claimed in claim 6, is characterized in that,
Described electric heating and thermal insulation device comprises: the one or more electric heating bars that extend along the periphery of described check-out console.
8. test kit as claimed in claim 1, is characterized in that, further comprises:
Shading valve, it is installed to the described lid with transparent region slidably, blocks described transparent region and when in open site, expose described transparent region when time in the closed position.
9. test kit as claimed in claim 1, is characterized in that, described check-out console is installed to described box body by a rotating shaft, and described check-out console can make to detect shrinkage pool from transferring to upward downward around described rotating shaft rotation.
10. test kit as claimed in claim 1, is characterized in that, further comprises:
Centrifuge tube bracing frame, it clamps described centrifuge tube, and when in holding state, described centrifuge tube is remained on to vertical position.
CN201420296925.0U 2014-05-29 2014-05-29 Test kit Expired - Fee Related CN203923196U (en)

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Application Number Priority Date Filing Date Title
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106239456A (en) * 2016-07-17 2016-12-21 佛山市大臻医学检验所有限公司 Portable high-efficiency detection kit
CN111527193A (en) * 2017-12-13 2020-08-11 株式会社日立高新技术 Antibacterial agent introduction plate for bacteriological examination and transparent plate
CN115092504A (en) * 2022-06-28 2022-09-23 温州医科大学 Diagnostic kit
CN116574595A (en) * 2023-05-23 2023-08-11 青岛中创汇科生物科技有限公司 Drug sensitivity detection kit

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106239456A (en) * 2016-07-17 2016-12-21 佛山市大臻医学检验所有限公司 Portable high-efficiency detection kit
CN111527193A (en) * 2017-12-13 2020-08-11 株式会社日立高新技术 Antibacterial agent introduction plate for bacteriological examination and transparent plate
CN111527193B (en) * 2017-12-13 2024-04-05 株式会社日立高新技术 Antibacterial agent introduction plate for bacterial examination and transparent plate
CN115092504A (en) * 2022-06-28 2022-09-23 温州医科大学 Diagnostic kit
CN116574595A (en) * 2023-05-23 2023-08-11 青岛中创汇科生物科技有限公司 Drug sensitivity detection kit

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20141105

Termination date: 20150529

EXPY Termination of patent right or utility model