CN203898495U - Embryo transfer device for rat and mouse embryo transfer - Google Patents
Embryo transfer device for rat and mouse embryo transfer Download PDFInfo
- Publication number
- CN203898495U CN203898495U CN201420150543.7U CN201420150543U CN203898495U CN 203898495 U CN203898495 U CN 203898495U CN 201420150543 U CN201420150543 U CN 201420150543U CN 203898495 U CN203898495 U CN 203898495U
- Authority
- CN
- China
- Prior art keywords
- embryo
- embryo transfer
- collecting tube
- transfer device
- syringe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 71
- 238000012546 transfer Methods 0.000 title claims abstract description 14
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims description 4
- 238000002161 passivation Methods 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 abstract description 4
- 230000006378 damage Effects 0.000 abstract description 3
- 210000003101 oviduct Anatomy 0.000 abstract description 2
- 210000002257 embryonic structure Anatomy 0.000 abstract 3
- 208000027418 Wounds and injury Diseases 0.000 abstract 1
- 239000011521 glass Substances 0.000 abstract 1
- 208000014674 injury Diseases 0.000 abstract 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 7
- 210000004291 uterus Anatomy 0.000 description 6
- 238000000034 method Methods 0.000 description 4
- 241000973497 Siphonognathus argyrophanes Species 0.000 description 3
- 239000012531 culture fluid Substances 0.000 description 3
- 229920001971 elastomer Polymers 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 210000005000 reproductive tract Anatomy 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- QGMRQYFBGABWDR-UHFFFAOYSA-M Pentobarbital sodium Chemical compound [Na+].CCCC(C)C1(CC)C(=O)NC(=O)[N-]C1=O QGMRQYFBGABWDR-UHFFFAOYSA-M 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000806 elastomer Substances 0.000 description 1
- 239000013536 elastomeric material Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000003692 ilium Anatomy 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 229960002275 pentobarbital sodium Drugs 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000004340 zona pellucida Anatomy 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an embryo transfer device for rat and mouse embryo transfer. The embryo transfer device comprises an embryo collecting tube, a flexible joint and a hose connected with an injector, wherein the injector is connected with the embryo collecting tube through the flexible joint and the hose, so that the force of the injector and the embryo collecting tube is buffered; the middle section of the embryo collecting tube is grilled to reach the temperature of 110-120 DEG C and the embryo collecting tube has a blunt tip end opening of which the inner diameter phi is 300-400 mu m, so as to reduce the injury to wombs, oviducts and embryos and prevent the embryos from being lost. The embryo transfer device is good in sterile control and ensures stable quality, the embryos are conveniently observed and collected under an inverted microscope or sight glass and are conveniently transferred, the preparation is simple and the cost is low.
Description
Technical field
This utility model belongs to animal embryo and transplants the embryo transplanter of use, the embryo transplanter that particularly large and small Mus embryo transfer is used.
Background technology
Large and small Mus is as a kind of important laboratory animal of exploring gene function in developmental biology and disease research, large and small musculus cdna group is carried out to genetic modification and relate to embryo transfer (Embryo Transfer, ET, claim again zygote transplation), be transplanted in the receptor Mus reproductive tract that physiological status is identical by the body early embryo of the inside and outside production of its body, make it to continue to develop into normal individual.Embryo's the whether successful pseudo-fetus of quality, receptor Mus, the factor such as method and skilled operation degree of transplanting are depended in embryo transfer success or not; in this process; adopt the embryo transplanter of suitable aperture, handled easily for successfully collecting and protect body early embryo, and embryo transfer is particularly important to receptor Mus reproductive tract.
Commercially available embryo transplantation tube is generally imported product at present, and price is more expensive.Also the mouth suction pipe that has researcher to use Pasteur to manage making operates, but the collecting amount of mouthful more difficult control embryo liquid of suction pipe, and preparation is difficult to specification.
Summary of the invention
This utility model aims to provide that a kind of work efficiency is high, steady quality, easy to use, aseptic controlling good and prepare easy embryo transplanter.
Described in embryo transplanter of the present utility model, object reaches with the following methods:
It is made up of embryo collection pipe (2), connecting hose (3), flexible pipe (4), syringe (5), wherein:
The long capillary glass-tube of 12cm that embryo collection pipe (2) is crossed for ethane via epoxyethane sterilization, 1/3 ~ 1/2 position of its tube most advanced and sophisticated mouthful (1) is 110 ° ~ 120 °, tip port (1) is the passivation mouth of internal diameter Φ 300～400 μ m, so that manually draw embryo under inverted microscope or stereoscope;
Embryo collection pipe (2) is connected with one end (41) of flexible pipe (4) by connecting hose (3), and the other end (42) of flexible pipe (4) connects the entrance port of 1～4mL syringe (5).
The syringe (5) of described embryo transplanter is 1mL.
Beneficial effect and technological progress that the embryo transplanter of this utility model has are:
Its embryo collection pipe crest truncation is that internal diameter is that Φ 300～400 μ m can reduce tip port to uterus and oviducal damage, avoids embryo to cause damage, again can be because of the excessive loss that causes embryo in migration process of bore.The effective connecting hose of syringe and embryo collection and hose connection, the connecting hose of connection and flexible pipe can play cushioning effect.Operating distance between object lens and the object stage of inverted microscope is 6 ~ 7cm, 1/3 ~ 1/2 section, embryo collection pipe tube mouth tip scorches into be had 110 ° ~ hexagonal angle degree and is conducive to directly collect embryo at inverted microscope with after looking Microscopic observation embryo's morphosis, carry out the uterine transplantation of large and small Mus, the embryo transplanter of other types is suitable for carrying out under the stereoscope of operating distance 6cm ~ 12cm the operation of embryo collection.
This utility model preparation method is simple and easy can be illustrated in following detailed description of the invention, and its steady quality, easy to use, aseptic controlling good and work efficiency high is seen in following detailed description of the invention purgation operational instances.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model, and figure comprises: tip port one, embryo collection pipe 2, connecting hose 3, flexible pipe 4, syringe 5, and the two ends 41,42 of flexible pipe 4.
Detailed description of the invention
Embodiment 1:
The long capillary glass-tube of 12cm of the internal diameter 1mm that embryo collection pipe 1 is crossed for ethane via epoxyethane sterilization, most advanced and sophisticated 1/3 position of the tube mouth of capillary glass-tube is 120 ° so that manually draw embryo under inverted microscope or stereoscope, and most advanced and sophisticated 1 internal diameter of the mouth of pipe of passivation is Φ 350 μ m
Embryo collection pipe 2 is connected with one end 41 of flexible pipe 4 by connecting hose 3, and the other end 42 of flexible pipe 4 connects the entrance port of 1mL syringe 5.
Above connecting hose 3 is elastomeric material with flexible pipe 4.
Embodiment 2:
Prepare embryo collection pipe: the capillary glass-tube that the approximately 12cm of the oxirane disinfection sterilizing of learning from else's experience is long, heats approaching tip position alcohol burner; When heating part rubicundity and become very soft after, remove flame, rapidly with pincet by its most advanced and sophisticated drawing-down, prevent from melting and stop up, external force is broken into the mouth of pipe 1 of flat and passivation, and most advanced and sophisticated 1 internal diameter of the mouth of pipe is Φ 300～400 μ m, after cooling, again 1/3 place at the tube mouth tip 1 of capillary glass-tube is scorched and has 110～hexagonal angle degree, this angle is convenient under inverted microscope and stereoscope, manually draw embryo, has completed the preparation of embryo collection pipe 1;
Prepare embryo transplanter: joint one end 42 of rubber tube 4 is connected to the entrance port of syringe 5, the other end 41 of rubber tube 4 is connected with embryo collection pipe 2 by elastomer connector 3.
Operational instances:
Utilize prepared embryo transplanter directly under inverted microscope or stereoscope, with the embryo collection pipe of syringe UNICOM, manually first draw the long culture fluid of about 0.5cm (not containing embryo), and then inhale the air section of 0.5cm, finally draw the standard compliant embryo of quality who selects.
Pseudo-fetus mice is injected on super bacterium workbench after 1% pentobarbital sodium solution 0.2ml anesthesia, carry out Baoding, ventricumbent position, with the back of iodine tincture cotton wiping mice, from ilium 1.5cm with from center line 1cm place, back, cut off dorsal body setae, use alcohol swab wiping, see out the otch of 1cm with little shears, see white adipose piece, gently draw and draw ovary, fallopian tube and uterus, stab an aperture in pipe junction, palace with 26gauge syringe needle, confirm that pin enters in intracavity instead of Uterus wall, extract pin, to embryo's the embryo collection grafts about 5mm in hole insertion uterus be thus housed, utilize syringe thrust will contain gently embryo's solution segments and throw uterus into until air section, leave standstill 20s left and right, rear sew up wound.Notice that the culture fluid containing embryo sucking in embryo collection pipe must lack, the culture fluid of squeezing into uterus also must lack, and will select the embryo transfer that zona pellucida is complete.
Claims (2)
1. the embryo transplanter that large and small Mus embryo transfer is used, this embryo transplanter is made up of embryo collection pipe (2), connecting hose (3), flexible pipe (4), syringe (5), it is characterized in that:
The long capillary glass-tube of 12cm that embryo collection pipe (2) is crossed for ethane via epoxyethane sterilization, 1/3 ~ 1/2 position of its tube most advanced and sophisticated mouthful (1) is 110 ° ~ 120 °, tip port (1) is the passivation mouth of internal diameter Φ 300～400 μ m, so that manually draw embryo under inverted microscope or stereoscope;
Embryo collection pipe (2) is connected with one end (41) of flexible pipe (4) by connecting hose (3), and the other end (42) of flexible pipe (4) connects the entrance port of 1～4mL syringe (5).
2. embryo transplanter according to claim 1, is characterized in that syringe (5) is 1mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420150543.7U CN203898495U (en) | 2014-03-31 | 2014-03-31 | Embryo transfer device for rat and mouse embryo transfer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420150543.7U CN203898495U (en) | 2014-03-31 | 2014-03-31 | Embryo transfer device for rat and mouse embryo transfer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203898495U true CN203898495U (en) | 2014-10-29 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420150543.7U Expired - Lifetime CN203898495U (en) | 2014-03-31 | 2014-03-31 | Embryo transfer device for rat and mouse embryo transfer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203898495U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104771212A (en) * | 2015-04-23 | 2015-07-15 | 南京爱贝生物科技有限公司 | Trace embryo and gamete transfer method |
| WO2016101226A1 (en) * | 2014-12-25 | 2016-06-30 | 河北农业大学 | Embryo transplantation device and operation method thereof |
| CN109223143A (en) * | 2018-11-09 | 2019-01-18 | 潍坊护理职业学院 | A kind of embryo transplanter for test-tube baby |
| CN110013292A (en) * | 2019-05-21 | 2019-07-16 | 安徽农业大学 | A kind of shifting embryo needle being able to maintain that blastaea stereochemical structure |
| CN110269720A (en) * | 2019-07-17 | 2019-09-24 | 山西医科大学 | A kind of fixed pin and preparation method thereof for mice embryonic amniotic cavity microinjection |
-
2014
- 2014-03-31 CN CN201420150543.7U patent/CN203898495U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016101226A1 (en) * | 2014-12-25 | 2016-06-30 | 河北农业大学 | Embryo transplantation device and operation method thereof |
| CN104771212A (en) * | 2015-04-23 | 2015-07-15 | 南京爱贝生物科技有限公司 | Trace embryo and gamete transfer method |
| CN109223143A (en) * | 2018-11-09 | 2019-01-18 | 潍坊护理职业学院 | A kind of embryo transplanter for test-tube baby |
| CN110013292A (en) * | 2019-05-21 | 2019-07-16 | 安徽农业大学 | A kind of shifting embryo needle being able to maintain that blastaea stereochemical structure |
| CN110269720A (en) * | 2019-07-17 | 2019-09-24 | 山西医科大学 | A kind of fixed pin and preparation method thereof for mice embryonic amniotic cavity microinjection |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20141029 |
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| CX01 | Expiry of patent term |