CN203790958U - Automatic multi-channel pipettor of real-time detection of suction head - Google Patents

Automatic multi-channel pipettor of real-time detection of suction head Download PDF

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Publication number
CN203790958U
CN203790958U CN201420076632.1U CN201420076632U CN203790958U CN 203790958 U CN203790958 U CN 203790958U CN 201420076632 U CN201420076632 U CN 201420076632U CN 203790958 U CN203790958 U CN 203790958U
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China
Prior art keywords
suction nozzle
liquid
contact block
real
transfering device
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Expired - Lifetime
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CN201420076632.1U
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Chinese (zh)
Inventor
王炜
何农跃
邬燕琪
陈柱
夏云
马嫚
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Anhui Zhongke Spring Valley Laser Industry Technology Research Institute Co Ltd
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Nanjing Institute of Advanced Laser Technology
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Priority to CN201420076632.1U priority Critical patent/CN203790958U/en
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Abstract

The utility model discloses an automatic multi-channel pipettor of real-time detection of a suction head. The automatic multi-channel pipettor comprises a pipetting device, the suction head mounted at the lower end of the pipetting device, a frame for fixing the pipetting device and a position detection device, wherein a through hole is formed in the bottom surface of the frame; a touch block which can slide up and down sleeves the pipetting device; a protuberance extending to the edge of the through hole is arranged on the outer wall of the touch block; the touch block at the lower side of the protuberance passes through the through hole along with the pipetting device and extends downwards; the lower end of the touch block is in contact with the suction head; the position detection device is connected with the touch block. According to the automatic multi-channel pipettor, whether the suction head is assembled or fallen off is detected in real time by detecting the position of the touch block; the automatic multi-channel pipettor can respond correspondingly as long as the suction head is not assembled in place, i.e., the artificial monitoring is replaced. Thus, the working efficiency is improved and the cost is lowered.

Description

Detect in real time the multichannel automatic pipettor of suction nozzle
Technical field
The utility model relates to micropipette instrument field, relates in particular to a kind of multichannel automatic pipettor of real-time detection suction nozzle.
Background technology
Biomation work station can be realized high flux imbibition, move liquid and liquid feeding, improved widely the preparation efficiency of sample, as a core component---the Multi-channel liquid transfer device on biomation work station, it need to change suction nozzle frequently, in the preparation process of sample, if some suction nozzles do not load and put in place or come off in the course of the work, all can destroy the preparation process of whole sample.
Multi-channel liquid transfer device generally adopts air displacement formula piston structure to realize imbibition and liquid feeding process, Multi-channel liquid transfer device generally needs the passage of a plurality of piston body structures, the restriction that is subject to processing and assembles, be difficult to guarantee that each piston body can be assembled to same level, will inevitably have influence on like this each passage suction nozzle and load rear sealing, how to design and can reduce matching requirements, and can improve the difficult point that each passage imbibition precision is also needs solution.
Utility model content
Whether utility model object: the utility model aims to provide a kind of multichannel automatic pipettor of real-time detection suction nozzle, can detect in real time suction nozzle and come off, and adopt the independently design of piston body structure passage can greatly reduce the requirement of assembling.
Technical scheme: a kind of multichannel automatic pipettor of real-time detection suction nozzle, comprise liquid-transfering device, be arranged on the suction nozzle of liquid-transfering device lower end and the fixing framework of liquid-transfering device, it is characterized in that, framework bottom surface offers through hole, on liquid-transfering device, be arranged with the contact block that can slide up and down, contact block outer wall is provided with the projection of extending to through hole edge, and the contact block of projection downside is with liquid-transfering device through through hole and to downward-extension, and the lower end of contact block contacts with suction nozzle; Also comprise position detecting device, position detecting device is connected with contact block.
Position detecting device comprises the battery lead plate being fixed in framework and is located at the electrode contact of end, contact block upper end, the through hole that battery lead plate is provided with a plurality of sensing electrodes and passes for liquid-transfering device; When contact block slides up and down, the sensing electrode on described electrode contact electric conduction pole plate.
Battery lead plate connects by winding displacement the electrode that framework end face is installed.Electrode is the electrode of golden finger type.The electrode of golden finger type, for coordinating with biomation work station, provides the status signal of suction nozzle to biomation work station.
At the sheathed top, outside of liquid-transfering device spring, the upper surface of top spring contacts with the lower surface of the gripper shoe that install position detecting device top, and the lower surface of top spring contacts with the upper surface of contact block.When suction nozzle is in place, contact block upward sliding in suction nozzle holder, top spring compressive deformation, and because the frictional force between suction nozzle and liquid-transfering device lower end is greater than the restoring force of top spring, suction nozzle can not come off; If but suction nozzle does not load and puts in place, the restoring force of top spring makes contact block down sliding, get back on liquid-transfering device slidably extreme lower position.
Suction nozzle inwall is provided with projection, and the upside of projection contacts with the lower surface of liquid-transfering device.
Liquid-transfering device lower end is a cone, and annular groove is offered in the lower end of cone, is placed with lower O type circle in annular groove, and lower O type circle contacts with the inwall of suction nozzle.Thereby the inwall of suction nozzle extruding O type circle is realized the sealing that moves liquid passage.
Suction nozzle is equipped with filter core, for preventing that reagent molecule or vapour colloidal sol from diffusing in liquid-transfering device, can avoid polluting.
Operation principle: at the suction nozzle of multichannel automatic pipettor that detects in real time suction nozzle not under installment state, contact block extreme lower position slidably on liquid-transfering device; When suction nozzle is packed into from the lower end of liquid-transfering device, the upper surface of suction nozzle contacts at first with the lower surface of contact block, now suction nozzle holder contact block to top offset, the electrode contact of contact block and the battery lead plate of top are contacted, the electrode of battery lead plate respective channel will be switched on, Continuity signal is delivered to the golden finger electrode of framework end face upper right side by right side winding displacement, work station can read by golden finger electrode the signal of telecommunication that suction nozzle packs into, thereby can judge that suction nozzle loading puts in place.Now, the inwall of suction nozzle can contact with the lower O type circle of liquid-transfering device, thereby and the lower O type circle of extruding realize the sealing of bottom; With the top spring compressive deformation of end in contact on contact block, but because the frictional force between suction nozzle and liquid-transfering device lower end is greater than the restoring force of top spring, suction nozzle can not come off; If suction nozzle does not load and puts in place, contact block is subject to the effect of top spring restoring force to get back to extreme lower position, now, electrode contact and then moves down together, disconnects and contacting with battery lead plate, and Continuity signal interrupts, work station cannot obtain Continuity signal from golden finger electrode, sends the alarm that suction nozzle unloaded puts in place.
Beneficial effect: the utility model is by detecting the position of contact block, whether realize the online suction nozzle that detects in real time loads and comes off, once it is not in place that suction nozzle loads, the multichannel automatic pipettor that detects suction nozzle in real time can be made corresponding reaction, replaced manual monitoring, improve operating efficiency, saved labor cost.And each liquid-transfering device employing dynamic fit, separate, can not be subject to the impact of other liquid-transfering device, be convenient to change; In addition, the utility model also possesses simple in structure, easily assembling, and good sealing effect, long service life, can keep the advantages such as precision of imbibition and liquid feeding for a long time.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
The specific embodiment
As shown in Figure 1, a multichannel automatic pipettor for real-time detection suction nozzle, comprises linear electric motors 101, electric machine support 102, upper cover plate 201, lower cover 202, side plate 203, drive plate 301, gripper shoe 302, support bar 303, battery lead plate 401, contact block 402, top spring 403, winding displacement 404, piston rod 501, spring 502, trim ring 503, upper O type circle 504, piston cylinder 505, lower O type circle 506, suction nozzle 601, filter core 602.
Linear electric motors 101 are arranged on electric machine support 102, and linear electric motors 101 are fixing shaft type linear stepping motor, and driving shaft moves up and down vertically, does not rotate.Adopt fixedly shaft type linear stepping motor, drives structure design is simplified greatly.Electric machine support 102 is arranged on upper cover plate 201.There is the electrode of golden finger type in the upper right side of upper cover plate 201, for coordinating with biomation work station, provides the status signal of suction nozzle 601 to biomation work station.Between the end of 203, two side plate 203 downsides of upper cover plate 201 both sides installation side plates, lower cover 202 is installed, upper cover plate 201, lower cover 202, the common formation framework of side plate 203; In framework, be in turn provided with drive plate 301, gripper shoe 302 and battery lead plate 401 parallel with upper cover plate 201, all offers circular hole on four angles of cover plate 201, drive plate 301, gripper shoe 302, battery lead plate 401 and lower cover 202; Support bar 303 is fixed on drive plate 301, gripper shoe 302 and battery lead plate 401 between upper cover plate 201 and lower cover 202 through each circular hole; Wherein upper cover plate 201, gripper shoe 302, battery lead plate 401 and lower cover 202 are fixedly connected with support bar 303; Drive plate 301 is flexibly connected with support bar 303, and drive plate 301 can be done axially and move up and down along support bar 303.Support bar 303 materials are hard, wear-resistant metal, and polishing is done on surface and chromium plating is processed, and guarantees its smooth surface.Through hole is offered in upper cover plate 201 centers, and the driving shaft of linear electric motors 101 is connected with drive plate 301 through the through hole of upper cover plate 201, and the motion of the driving shaft of linear electric motors 101 can drive drive plate 301 to move up and down along support bar 303.
The multichannel automatic pipettor of the real-time detection suction nozzle in the present embodiment is 8 passages, and each passage is provided with a liquid-transfering device, moves liquid dress and comprises piston rod 501, spring 502, trim ring 503, upper O type circle 504, piston cylinder 505, lower O type circle 506.Piston rod 501 is connected to drive plate 301 lower surfaces, and spring 502 is enclosed within the outer wall of piston rod 501, and piston rod 501 is set in piston cylinder 505, and piston cylinder 505 is fixed in gripper shoe 303.Piston cylinder 505 adopts stainless steel material, and the diameter of piston cylinder 505 upper ends is greater than the diameter of stage casing cylindrical shell, and piston cylinder 505 ends, upper end offer groove, places successively upper O type circle 504 and trim ring 503 in this groove; In gripper shoe 302, there are 8 identical circular holes of diameter, the center of circle of circular hole point-blank, the diameter of circular hole is identical with the external diameter of piston cylinder 505 stage casing cylindrical shells, when piston cylinder 505 inserts from gripper shoe 302 tops, because piston cylinder 505 upper end diameter are greater than the diameter of gripper shoe 302 circular holes, the bottom surface of piston cylinder 505 upper ends forms and contacts with gripper shoe 302 upper surfaces, and this contact-making surface is piston supporting surface, and piston cylinder 505 is supported by gripper shoe 303.
The outer wall of piston cylinder 505 stage casing cylindrical shells overlaps successively top spring 403 and contact block 402, and the upper surface of top spring 403 contacts with gripper shoe 303 lower surfaces, and the lower surface of top spring 403 contacts with the upper surface of contact block 402.The material of contact block 402 is potteries, is shaped as cylindricly, and contact block 402 is matched in clearance with piston cylinder 505, and contact block 402 can slide up and down along piston cylinder 505.The upper surface of contact block 402 scribbles conducting resinl, and there are two projections the upper surface of contact block 402 for electrode contact, and electrode contact top is provided with battery lead plate 401, battery lead plate 401 is fixing by support bar 303, battery lead plate 401 is PCB circuit boards, which is provided with a plurality of sensing electrodes, shares a negative or positive electrode.Battery lead plate 401 is connected with the electrode of golden finger type on upper cover plate 201 by right side winding displacement 404; Battery lead plate 401 also has 8 identical circular holes of diameter, and point-blank, circular hole passes for the piston cylinder 505 that has been nested with top spring 403 in the center of circle of circular hole.On lower cover 202, have 8 diameter same via, point-blank, the diameter of lower cover 202 through holes is identical with the external diameter of contact block 402 in the center of circle of through hole, and cover has the piston cylinder 505 of contact block 402 to continue to downward-extension through lower cover 202 through holes.The outer wall of contact block 402 is provided with flange, flange stretches out from through hole edge, when contact block 402 penetrates from the top of lower cover 202 through holes, flange is blocked in part contact block 402 on lower cover 202, further also defines contact block 402 in the position of piston cylinder 505 down slidings.
Piston cylinder 505 lower ends are that tapering is the cone of 17 °, and annular groove is offered in the lower end of cone, are placed with lower O type circle 506 in annular groove; The material of upper O type circle, lower O type circle is elastic caoutchouc wear-resisting, acid and alkali-resistance, such as fluorine unpigmented rubber etc.Suction nozzle 601 is equipped with in the lower end of piston cylinder 505, and the inwall tapering of suction nozzle 601 is 17 °, and the length of suction nozzle 601 is 40mm, and the capacity of suction nozzle 601 is 50 μ l.The upper inside wall size of suction nozzle 601 and piston cylinder 505 lower end cones measure-alike, can increase the laminating of suction nozzle and piston cylinder.Suction nozzle 601 inwalls are provided with projection, and the lower surface of piston cylinder 505 contacts with the upside of suction nozzle 601 inner processes, and the inwall of suction nozzle 601 contacts with the lower O type circle 506 of piston cylinder, thus and the lower O type circle 506 realization sealings of extruding.Suction nozzle 601 materials are polypropylene, and silanization processing is carried out on surface, can reduce extension liquid.Suction nozzle 601 is also equipped with filter core 602, can prevent that reagent molecule or vapour colloidal sol from diffusing in piston cylinder 505, avoids polluting.
During installation, piston rod 501 from lower end pack into successively spring 502, trim ring 503, O type circle 504, then be inserted in piston cylinder 505, upper O type circle 504, trim ring 503 are placed in the groove on piston cylinder top; Piston rod 501 contacts with drive plate 301 lower surfaces, and the center, upper surface of drive plate 301 is connected with the driving shaft of linear electric motors 101.Piston cylinder 505, from gripper shoe 302 upper ends pack into, then after top spring 403, contact block 402 are installed on piston cylinder 505 successively, then is completed to cooperation through the circular hole of battery lead plate 401 and lower cover 202.
Do not installing under the state of suction nozzle 601, contact block 402 is affiliated on lower cover 202, contact block 402 extreme lower position slidably on piston cylinder 505, when suction nozzle 601 is packed into from the lower end of piston cylinder, the upper surface of suction nozzle 601 contacts at first with the lower surface of contact block 402, continue upwards to pack into, until the lower surface of piston cylinder 505 contacts with the upside of suction nozzle 601 inner processes, till can not upwards packing into again, now suction nozzle 601 is asking contact block 402 to top offset, the electrode contact of contact block 402 and the battery lead plate of top 401 are contacted, the electrode of battery lead plate 401 respective channel will be switched on, Continuity signal is delivered to the golden finger electrode of upper cover plate 201 upper right sides by right side winding displacement, work station can read by golden finger electrode the signal of telecommunication that suction nozzle 601 packs into, thereby the suction nozzle that can judge loads and puts in place.Now, the inwall of suction nozzle 601 can contact with the lower O type circle 506 of piston cylinder, thereby and the lower O type circle 506 of extruding realize the sealing of bottom; With top spring 403 compressive deformations of end in contact on contact block 402, but because the frictional force between suction nozzle 601 and piston cylinder 505 lower ends is greater than the restoring force of top spring 403, suction nozzle 601 can not come off; If suction nozzle 601 does not load and puts in place, contact block 402 is subject to the effect of top spring 403 restoring forces to get back to extreme lower position, now, electrode contact and then moves down together, disconnect and contacting with battery lead plate 401, Continuity signal interrupts, and work station cannot obtain Continuity signal from golden finger electrode, sends the alarm that suction nozzle unloaded puts in place.The multichannel automatic pipettor of real-time detection suction nozzle described in the utility model, by detecting the position of contact block 402, just can judge whether suction nozzle 601 comes off.
The multichannel automatic pipettor course of work that detects in real time suction nozzle is as follows, when the driving shaft of linear electric motors 101 moves down, drive drive plate 301 to move downward, drive plate 301 drives a plurality of piston rods 501 to move down, piston rod 501 is discharged the gas in piston cylinder 505 from the lower end of suction nozzle 601, then the suction nozzle on pipettor 601 is inserted in reagent, linear electric motors 101 move upward and drive drive plate 301 to move up, piston rod 501 is subject to the effect of spring 502 restoring forces, along with drive plate 301 moves up together, piston cylinder 505 forms negative pressure, reagent will be drawn in suction nozzle 601, linear electric motors 101 again move down and the reagent being drawn in suction nozzle 601 can be discharged, by controlling the displacement of linear electric motors 101, can realize accurate imbibition and liquid feeding.

Claims (8)

1. a multichannel automatic pipettor that detects in real time suction nozzle, comprise liquid-transfering device, be arranged on the suction nozzle of liquid-transfering device lower end and the fixing framework of liquid-transfering device, it is characterized in that, framework bottom surface offers through hole, on liquid-transfering device, be arranged with the contact block that can slide up and down, contact block outer wall is provided with the projection of extending to through hole edge, and the contact block of projection downside is with liquid-transfering device through through hole and to downward-extension, and the lower end of contact block contacts with suction nozzle; Also comprise position detecting device, position detecting device is connected with contact block.
2. the multichannel automatic pipettor of real-time detection suction nozzle according to claim 1, it is characterized in that, described position detecting device comprises the battery lead plate being fixed in framework and is located at the electrode contact of end, contact block upper end, the through hole that battery lead plate is provided with a plurality of sensing electrodes and passes for liquid-transfering device; When contact block slides up and down, the sensing electrode on described electrode contact electric conduction pole plate.
3. the multichannel automatic pipettor of real-time detection suction nozzle according to claim 2, is characterized in that, described battery lead plate connects by winding displacement the electrode that framework end face is installed.
4. the multichannel automatic pipettor of real-time detection suction nozzle according to claim 3, is characterized in that, the electrode that described electrode is golden finger type.
5. the multichannel automatic pipettor of real-time detection suction nozzle according to claim 1, it is characterized in that, at the sheathed top, outside of described liquid-transfering device spring, the upper surface of top spring contacts with the lower surface of the gripper shoe that install position detecting device top, and the lower surface of top spring contacts with the upper surface of contact block.
6. the multichannel automatic pipettor of real-time detection suction nozzle according to claim 1, is characterized in that, described suction nozzle inwall is provided with projection, and the upside of projection contacts with the lower surface of liquid-transfering device.
7. the multichannel automatic pipettor of real-time detection suction nozzle according to claim 6, is characterized in that, described liquid-transfering device lower end is a cone, and annular groove is offered in the lower end of cone, is placed with lower O type circle in annular groove, and lower O type circle contacts with the inwall of suction nozzle.
8. the multichannel automatic pipettor of real-time detection suction nozzle according to claim 1, is characterized in that, described suction nozzle is equipped with filter core, for preventing that reagent molecule or vapour colloidal sol from diffusing in liquid-transfering device.
CN201420076632.1U 2014-02-21 2014-02-21 Automatic multi-channel pipettor of real-time detection of suction head Expired - Lifetime CN203790958U (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105854969A (en) * 2016-06-12 2016-08-17 齐齐哈尔医学院 5-50 microliter 60-sucker liquid transfer device
CN108273578A (en) * 2018-03-07 2018-07-13 广东顺德工业设计研究院(广东顺德创新设计研究院) Liquid-transfering device
CN109477853A (en) * 2016-07-22 2019-03-15 泰肯贸易股份公司 Liquid shifting equipment with suction pipette head detection unit and the method for detecting the suction pipette head at liquid shifting equipment
CN109988705A (en) * 2019-05-07 2019-07-09 刘晓 A kind of test-tube baby's micromanipulation ware special multichannel circular drop matrix preparing device
CN111974473A (en) * 2020-08-13 2020-11-24 温州市人民医院 Batch sample adding device

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105854969A (en) * 2016-06-12 2016-08-17 齐齐哈尔医学院 5-50 microliter 60-sucker liquid transfer device
CN105854969B (en) * 2016-06-12 2017-08-29 齐齐哈尔医学院 5 50 microlitre of 60 suction nozzle pipettor
CN109477853A (en) * 2016-07-22 2019-03-15 泰肯贸易股份公司 Liquid shifting equipment with suction pipette head detection unit and the method for detecting the suction pipette head at liquid shifting equipment
CN109477853B (en) * 2016-07-22 2023-02-17 帝肯贸易股份公司 Pipetting device with a pipette tip detection unit and method for detecting pipette tips at a pipetting device
CN108273578A (en) * 2018-03-07 2018-07-13 广东顺德工业设计研究院(广东顺德创新设计研究院) Liquid-transfering device
CN108273578B (en) * 2018-03-07 2024-03-26 广东顺德工业设计研究院(广东顺德创新设计研究院) Pipetting device
CN109988705A (en) * 2019-05-07 2019-07-09 刘晓 A kind of test-tube baby's micromanipulation ware special multichannel circular drop matrix preparing device
CN111974473A (en) * 2020-08-13 2020-11-24 温州市人民医院 Batch sample adding device

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Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20231205

Address after: 241200 Chungu 3D Printing Industrial Park, Fanchang Economic Development Zone, Wuhu City, Anhui Province

Patentee after: Anhui Zhongke Spring Valley Laser Industry Technology Research Institute Co.,Ltd.

Address before: 210038 18 Xinghe Road, Nanjing Economic Development Zone, Nanjing, Jiangsu

Patentee before: NANJING INSTITUTE OF ADVANCED LASER TECHNOLOGY

CX01 Expiry of patent term
CX01 Expiry of patent term

Granted publication date: 20140827