CN203700356U - Defoaming paddle and defoaming device - Google Patents
Defoaming paddle and defoaming device Download PDFInfo
- Publication number
- CN203700356U CN203700356U CN201420074162.5U CN201420074162U CN203700356U CN 203700356 U CN203700356 U CN 203700356U CN 201420074162 U CN201420074162 U CN 201420074162U CN 203700356 U CN203700356 U CN 203700356U
- Authority
- CN
- China
- Prior art keywords
- froth breaking
- defoaming
- oar
- blade
- paddle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000004113 cell culture Methods 0.000 claims abstract description 17
- 210000004102 animal cell Anatomy 0.000 claims abstract description 15
- 230000005540 biological transmission Effects 0.000 claims abstract description 7
- 238000012423 maintenance Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 238000003756 stirring Methods 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000006260 foam Substances 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 235000011089 carbon dioxide Nutrition 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 239000013530 defoamer Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- 239000013543 active substance Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
Landscapes
- Degasification And Air Bubble Elimination (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model belongs to the field of biologic machinery, and particularly relates to a defoaming paddle and a defoaming device. The defoaming paddle is mainly applied in a biological reactor for animal cell culture, and comprises a deforaming paddle, a fixing ring and a fixing fitting, wherein the fixing ring is fixedly connected to the middle of the defoaming paddle, and the sawteeth are arranged at the bottom of the defoaming paddle. The defoaming device is mainly applied in the biological reactor for animal cell culture, and comprises a power device, a rotary shaft and the defoaming paddle, wherein the rotary shaft is fixedly connected with the defoaming paddle via the fixing fitting, and the bottom of the rotary shaft is in transmission connection with the power device. The defoaming paddle and the defoaming device have the advantages of simple structure, convenience for mounting and maintenance, low cost, high safety and the like.
Description
Technical field
The utility model belongs to bio-mechanical field, is specifically related to a kind of froth breaking oar and defoaming device.
Background technology
Animal cell large-scale high-density culture technology has obtained and has paid close attention to more and more widely and apply in the production of therapeutic type albumen.In cell large scale culturing process, biological reactor for cell culture is the key equipment in whole process, and it will provide suitable growing environment and determine the Quality and yield of cell cultures for cell.Possessing low shearing effect, preferably transmission effect and hydrodynamics according to the growth requirement of zooblast is this class reactor design or the mandatory principle of improvement.
Owing to containing the surfactants such as a certain amount of albumen, polysaccharide in fermented liquid, and the existence of cells, microcarrier etc. in a large number, under violent stirring and aeration condition, easily produce a large amount of foams, thereby cell is produced to damage, and hindered the gas-liquid mass transfer on top layer.In stirring reactor, the breather of bubbling style brings good dissolved oxygen mass transfer effect to system on the one hand, but also easily produce a large amount of foams, on the one hand cell is produced to damage, on the one hand, if foam gathers too much in media surface, top layer mass transfer is had to certain inhibition in addition, limit the delivery rate of oxygen, be unfavorable for the growth of cell.
At present, in stirring type bioreactor, general employing toward the mode of substratum interpolation defoamer avoided producing too much bubble, but defoamer belongs to tensio-active agent, may produce bad effect to purifying; Or adopt without the oxygen supply of bubbles for aeration device, limited in one's ability but this ventilating mode is supported, only adapt to small-scale cell cultures, be not suitable for mass cell and cultivate.
Utility model content
First object of the present utility model is for providing a kind of froth breaking oar, and described froth breaking oar is mainly used in animal cell culture bioreactor, has the features such as simple in structure, installation and maintenance convenient, cost is low, security is high.Concrete technical scheme is as follows:
A kind of froth breaking oar, comprises froth breaking blade, set collar and standing finish, and described set collar is fixed in the middle part of described froth breaking blade, and the bottom of described froth breaking blade is provided with sawtooth.
Further, the back side of described froth breaking blade is the one in inclined-plane, plane, cambered surface.
Further, described froth breaking oar is mainly used in animal cell culture bioreactor, and the diameter of described froth breaking blade is 75～90% of described animal cell culture bioreactor diameter.
Further, described froth breaking blade bottom tooth width is 1/20～1/10 of described froth breaking diameter of propeller blade.
Further, the tooth pitch between the adjacent sawtooth in described froth breaking blade bottom is 10～30mm.
Further, described standing finish comprises nut, clip and cutting ferrule.
Second object of the present utility model is for providing a kind of defoaming device, and described defoaming device is mainly used in animal cell culture bioreactor, and described defoaming device comprises power set, turning axle and above-mentioned froth breaking oar, wherein:
Described turning axle is fixedly connected with described froth breaking oar by described standing finish, and the bottom of described turning axle and described power set are in transmission connection.
Further, described power set is electric motor.
Compared with prior art, advantage is for the disclosed froth breaking oar of the utility model and defoaming device:
(1) simple in structure, installation and maintenance are convenient and cost is low, safe, especially meet the clean requirement of pharmaceutical industries, and independent installation, is easy to clean, and is applicable to the animal cell culture bioreactor of production for treating type protein drug;
(2) thering is preferably defoaming effect---froth breaking oar is fixed on stir shaft, rotate along with the rotation of stirring rake, most of bubble of liquid level is directly broken up, liquid level bottom bubble is arrived in edge, itself and tank inwall bump against, bubble breaking in knockout process, becomes liquid and is back in solution along tank skin;
(3) after bubble is broken up, liquid reactor middle part surface is exposed, and it contacts with gas, and dissolved oxygen transmission is strengthened, and carbonic acid gas volatilization is accelerated, and is conducive to Growth of Cells;
(4) after bubble is broken up, be brought to surperficial cell and get back to rapidly in liquid, recover growth, avoided the minimizing of cell.
Accompanying drawing explanation
Fig. 1 is that the back side is the froth breaking paddle structure schematic diagram on inclined-plane
Fig. 2 is defoaming device structural representation
Fig. 3 is that the back side is the froth breaking paddle structure schematic diagram of cambered surface
Fig. 4 is that the back side is the froth breaking paddle structure schematic diagram of plane
Embodiment
Describe below in conjunction with accompanying drawing
Specific embodiment 1
As shown in Figure 1, a kind of froth breaking oar, is mainly used in animal cell culture bioreactor, froth breaking oar comprises froth breaking blade 1, set collar 2 and standing finish 3, set collar 2 is fixed in the middle part of froth breaking blade 1, and the bottom of froth breaking blade 1 is provided with sawtooth, and the back side of froth breaking blade 1 is inclined-plane.The diameter of froth breaking blade 1 is 75% of animal cell culture bioreactor diameter, and froth breaking blade 1 bottom tooth width is 1/20 of its diameter, the tooth pitch 10mm between the froth breaking blade 1 adjacent sawtooth in bottom.Standing finish 3 comprises nut, clip and cutting ferrule.
As shown in Figure 2, a kind of defoaming device, is mainly used in animal cell culture bioreactor, and defoaming device comprises the froth breaking oar described in power set 4, turning axle 5 and Fig. 1, and froth breaking oar is fixed on turning axle 5 by set collar 2, standing finish 3.The bottom of turning axle 5 and power set 4 are in transmission connection, and power set 4 is electric motor.
When use, it is upper that froth breaking oar is fixed on the suitable position of described stir shaft (apart from substratum liquid level 0.5～1cm) by standing finish 3, and power set 4 drives turning axle 5 and drives the common unitary rotation of froth breaking blade 1.When defoaming device rotates, froth breaking blade 1 is smashed part bubble by shearing force, and part does not have broken bubble to rotate with the rotation of defoaming device, under centrifugal action, be thrown out of, then itself and tank inwall bump against, and bubble breaking in knockout process, becomes liquid and be back in solution along tank skin.Reactor part fluid level gauge broad in the middle exposes, and is beneficial to oxygen transmission and carbonic acid gas volatilization.
Specific embodiment 2
Roughly the same with specific embodiment 1, difference is only:
(1) as shown in Figure 3, the back side of froth breaking blade 1 is arc;
(2) diameter of froth breaking blade 1 is 90% of animal cell culture bioreactor diameter, and froth breaking blade 1 bottom tooth width is 1/10 of its diameter, the tooth pitch 30mm between the froth breaking blade 1 adjacent sawtooth in bottom.
Specific embodiment 3
Roughly the same with specific embodiment 1, difference is only:
(1) as shown in Figure 4, the back side of froth breaking blade 1 is plane;
(2) diameter of froth breaking blade 1 is 85% of animal cell culture bioreactor diameter, and froth breaking blade 1 bottom tooth width is 1/16 of its diameter, the tooth pitch 20mm between the froth breaking blade 1 adjacent sawtooth in bottom.
Claims (8)
1. a froth breaking oar, is characterized in that, comprises froth breaking blade, set collar and standing finish, and described set collar is fixed in the middle part of described froth breaking blade, and the bottom of described froth breaking blade is provided with sawtooth.
2. froth breaking oar as claimed in claim 1, is characterized in that, the back side of described froth breaking blade is the one in inclined-plane, plane, cambered surface.
3. froth breaking oar as claimed in claim 1, described froth breaking oar is mainly used in animal cell culture bioreactor, it is characterized in that, and the diameter of described froth breaking blade is 75 ~ 90% of described animal cell culture bioreactor diameter.
4. froth breaking oar as claimed in claim 1, is characterized in that, described froth breaking blade bottom tooth width is 1/20～1/10 of described froth breaking diameter of propeller blade.
5. froth breaking oar as claimed in claim 1, is characterized in that, the tooth pitch between the adjacent sawtooth in described froth breaking blade bottom is 10～30mm.
6. froth breaking oar as claimed in claim 1, is characterized in that, described standing finish comprises nut, clip and cutting ferrule.
7. a defoaming device, its characteristic is, comprises froth breaking oar claimed in claim 1, power set and turning axle, and described turning axle is fixedly connected with described froth breaking oar by described standing finish, and the bottom of described turning axle and described power set are in transmission connection.
8. defoaming device as claimed in claim 7, is characterized in that, described power set is electric motor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420074162.5U CN203700356U (en) | 2014-02-20 | 2014-02-20 | Defoaming paddle and defoaming device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420074162.5U CN203700356U (en) | 2014-02-20 | 2014-02-20 | Defoaming paddle and defoaming device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203700356U true CN203700356U (en) | 2014-07-09 |
Family
ID=51050820
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420074162.5U Expired - Lifetime CN203700356U (en) | 2014-02-20 | 2014-02-20 | Defoaming paddle and defoaming device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203700356U (en) |
-
2014
- 2014-02-20 CN CN201420074162.5U patent/CN203700356U/en not_active Expired - Lifetime
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: No.1 and No.2 Juli Road, No.280, Free Trade Pilot Area, Pudong New Area, Shanghai, 2003 Patentee after: TASLY PHARMACEUTICAL GROUP Co.,Ltd. Address before: 201203 No. 280 Curie Road, Zhangjiang High-tech Park, Pudong New Area, Shanghai Patentee before: SHANGHAI TASLY PHARMACEUTICAL Co.,Ltd. |
|
| CP03 | Change of name, title or address | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140709 |
|
| CX01 | Expiry of patent term |