CN203505347U - Novel device for researching larval diapause relief - Google Patents
Novel device for researching larval diapause relief Download PDFInfo
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- CN203505347U CN203505347U CN201320635844.4U CN201320635844U CN203505347U CN 203505347 U CN203505347 U CN 203505347U CN 201320635844 U CN201320635844 U CN 201320635844U CN 203505347 U CN203505347 U CN 203505347U
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- diapause
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- insects
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Abstract
The utility model discloses a device for simulating external environment of insects with larval diapause at diapause period. The purposes are as follows: firstly, the external environment of insects with larval diapause at diapause period is simulated, and the biological characteristics of the larva at diapause period are observed and grasped to provide first hand information for prediction and forecasting of the insects; secondly, the external environment of insects with larval diapause at diapause period is greatly met so as to reduce the error caused by human disturbance or mutual interference of insets to biological research of larval diapause. In order to achieve the above purposes, the device adopts the following technical scheme that the external environment of insects with larval diapause at diapause period is simulated. The device is characterized by comprising transparent cylinders, wherein the upper end and the lower end of each cylinder are plugged by cotton plugs, one diapause larvae is put in one cylinder, and the cylinders are vertically inserted in soil or inserted in floating plate pores. According to the device, required appliances are conveniently and easily obtained, the operation is simple, convenient and vivid, and the practicability is high.
Description
Technical field
The utility model relates to the insect with larval diapause, and simulation wild environment, with the developmental characteristic of insect between the duration of diapause of field of research larval diapause, is studied the developmental characteristic of larval diapause insect between duration of diapause under the permanent illumination condition of constant indoor temperature.
Background technology
Diapause is a type that animal is subject to inactive state that the induction of external environmental condition produces, it not only shows as the reduction of morphogenetic pause and physiological activity, and diapause Once you begin just must tide over certain phase or could finish after certain physiological change, by diapause status, can be divided into again more the summer residing season and survive the winter.For example, important pests Asiatic corn borer and the striped rice borer of cause harm corn and paddy rice, all survive the winter with larval diapause.In the time of in the winter time, larva keeps out the cold with diapause status, and between duration of diapause, does not need to take food.Diapause can occur in each stage of insect, can occur in ovum, larva (nymph), pupa and adult stage.Wherein with ovum, pupa and adult, enter the relatively good research of insect of diapause status; because it is external, there is diaphragm or approached maturity state; than being easier to, preserve; and with larva (nymph), enter the insect of diapause; its health is equivalent to directly be exposed in environment, and environment has a great impact its survival and smooth termination of diapause state.These have all determined that the external environment during larval diapause has directly affected the releasing of larval diapause, and at present the research method of this respect are existed to a lot of problems;
1, existing to being generally to look into or light trap lures and looks into by outdoor artificial stripping with larval diapause insect prediction, the two all exists corresponding weak point, when using outdoor artificial stripping checking method, need to expend a large amount of human and material resources, and use light trap to lure while looking into, be vulnerable to weather and artificial interference, easily cause the prediction of insect to produce larger deviation, be unfavorable for the effective prevention and control to insect;
2, for scientific research personnel, in desk research, with the diapause of the insect of larval diapause, remove and seem of crucial importance, and method is generally to allow diapause insect concentrate be in an external environment condition that relative moisture is higher, so not only can not effectively simulate external environment, but also ignored the direct feeding effect of insect to water, and the effect that has increased phase mutual interference between insect individuality, easily cause experimental error; Another kind method is that Diapause Larva single head is placed on to (as 24 orifice plates) in orifice plate, although this mode has solved the mutual interference effect between insect individuality, but ignored the direct feeding effect of insect to water completely, can make like this lethality of insect during termination of diapause increase, cause larger experimental bias.
Summary of the invention
The utility model, in order to solve the above-mentioned deficiency of art methods, provides a kind of new scheme.Above-mentioned purpose of the present utility model realizes by following technical scheme: simulation is with the external environment during the Insect Diapause of larval diapause, it is characterized in that: comprise transparent cylinder, its cylinder upper and lower side clogs with cotton plug, a cylinder is put a Diapause Larva, erects and is inserted in soil or is inserted in the kickboard hole floating in water.Described transparent cylinder can be the cylinder of various shapes, its thickness length can be determined according to the size of observed Diapause Larva, general thickness is creeped and is advisable with larva energy easy on and off, length is advisable with long 4 times of larva body, described cotton plug can be the stopper that various energy are ventilative and absorb water, and can make of absorbent cotton, and described kickboard can be the various objects (as cystosepiment) that can swim on the water surface, described hole, its diameter is inserted firmly not fall with cylinder.The utility model advantage is compared with prior art: upper end of the present utility model cotton plug can play ventilative and larva and bore moth (Diapause Larva can be chewed part cotton plug and become thread, thereby oneself is wrapped up) warming effect, lower end cotton plug can play the effect of simulating plant root system retaining, can guarantee the necessary ambient humidity of larval growth and directly water supply, transparent cylinder can play the effect of host's stem stalk, and convenient observation.We insert cylinder in soil, can direct modeling field Diapause Larva external environment; If cylinder is inserted in kickboard hole, the water supply of Diapause Larva can not only be provided, can also effectively carry out the various experiments of Diapause Larva under the permanent illumination condition of constant indoor temperature, also avoided phase mutual interference and the external environment influence between insect individuality simultaneously, and can accomplish large-scale repeated test, reduce experimental error, observed and recorded larva is in the situation of activity between duration of diapause and the position support situation after diapause releasing easily, can also to larva, add nutritive element by the form of nutrient solution if needed, larva is better survived.Cylinder of the present utility model and cotton plug have been simulated the diapause external environment of field larva effectively, also for indoor larval diapause, remove the research of testing simultaneously and have created good condition, and the required utensil of the utility model is conveniently easy to get, and method is simplified, easy and simple to handle;
For Corn Pests Asiatic corn borer, during larval diapause, larva inserted below in water, insert in soil and traditional diapause being placed in 24 orifice plates is removed success rate and is simply compared as follows:
| Insert in water | Insert in soil | Traditional approach (24 orifice plate) | |
| Success rate (%) is removed in diapause | 90.7±2.1 a | 88.0±2.0 a | 51.3±4.2 b |
Numeral in form (mean+SD), letter representation significant difference level, different letter representation significant differences.
Accompanying drawing explanation
Fig. 1 is that the utility model cylinder inserts the structural representation in kickboard hole;
Fig. 2 is that the utility model cylinder inserts the structural representation in soil.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in further detail:
As shown in Figure 1, a kind of for desk research the device with insect insecticidal properties between duration of diapause of larval diapause, it is characterized in that: comprise transparent cylinder 2, described cylinder 2 upper ends are plugged with cotton plug 1, and lower end is plugged with cotton plug 4, and the Diapause Larva 3 with larval diapause insect that needs are observed is placed in described cylinder 2, described with cotton plug 1,4, the cylinder 2 of Diapause Larva 3 is inserted in the hole 6 in kickboard 5, kickboard 5 is swum on horizontal plane 7, with water container 8 splendid attire water;
As shown in Figure 2, a kind of wild environment of simulating is with the insecticidal properties of insect between the duration of diapause of field of research larval diapause, it is characterized in that: comprise transparent cylinder 2, described cylinder 2 upper ends are plugged with cotton plug 1, lower end is plugged with cotton plug 4, the Diapause Larva 3 with larval diapause insect that needs are observed is placed in described cylinder 2, and by described, with cotton plug 1,4, the cylinder 2 of Diapause Larva 3 is inserted in 9 li, earth;
But, above-mentioned embodiment is exemplary, in order better to make those skilled in the art can understand this patent, can not be interpreted as it is this patent to be comprised to the restriction of scope: so long as according to spirit that this patent discloses do be anyly equal to change or modify, all fall into the scope that this patent comprises.
Claims (5)
1. study the new device that larval diapause is removed for one kind, it is characterized in that: comprise transparent cylinder, its cylinder upper and lower side clogs with cotton plug, and a transparent cylinder is put a Diapause Larva, erects and is inserted in soil or is inserted in the kickboard hole floating in water.
2. a kind of new device that larval diapause is removed of studying according to claim 1, it is characterized in that: cylindrical transparent, thickness diameter and length of tube are determined because of Diapause Larva size, and general thickness is creeped and is advisable with larva energy easy on and off, and length is advisable with 4 times of long left and right of larva body.
3. a kind of new device that larval diapause is removed of studying according to claim 1, is characterized in that: cylinder upper and lower side stopper is with function ventilative and water suction.
4. a kind of new device that larval diapause is removed of studying according to claim 1, is characterized in that: in the time of in direct insertion soil, make the top of cylinder lower end stopper be not less than native plane.
5. a kind of new device that larval diapause is removed of studying according to claim 1, is characterized in that: in the time of in the hole on inserting kickboard, kickboard hole is can fix cylinder, and after cylinder inserts, the top of its lower end stopper is not less than horizontal plane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320635844.4U CN203505347U (en) | 2013-10-16 | 2013-10-16 | Novel device for researching larval diapause relief |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320635844.4U CN203505347U (en) | 2013-10-16 | 2013-10-16 | Novel device for researching larval diapause relief |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203505347U true CN203505347U (en) | 2014-04-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320635844.4U Expired - Fee Related CN203505347U (en) | 2013-10-16 | 2013-10-16 | Novel device for researching larval diapause relief |
Country Status (1)
| Country | Link |
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| CN (1) | CN203505347U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108056071A (en) * | 2018-01-18 | 2018-05-22 | 中国农业科学院植物保护研究所 | A kind of Diapause inducement method of beautiful Chrysopa |
-
2013
- 2013-10-16 CN CN201320635844.4U patent/CN203505347U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108056071A (en) * | 2018-01-18 | 2018-05-22 | 中国农业科学院植物保护研究所 | A kind of Diapause inducement method of beautiful Chrysopa |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140402 Termination date: 20141016 |
|
| EXPY | Termination of patent right or utility model |