CN203385649U - Automatic imaging system for large-scale biological tissue serial section - Google Patents
Automatic imaging system for large-scale biological tissue serial section Download PDFInfo
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- CN203385649U CN203385649U CN201320129316.1U CN201320129316U CN203385649U CN 203385649 U CN203385649 U CN 203385649U CN 201320129316 U CN201320129316 U CN 201320129316U CN 203385649 U CN203385649 U CN 203385649U
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Abstract
The utility model provides an imaging system for a large-scale biological tissue serial section. The imaging system comprises an image obtaining unit used for obtaining a digital image of the serial section, a splicing unit used for carrying out two-dimensional splicing on the image to form an integral image of the large-scale section, and a control unit used for controlling the image obtaining unit to automatically image and controlling the splicing unit to automatically splice the image. The control unit is used for controlling the image obtaining unit to automatically and continuously obtain the image of the section and controlling the splicing unit to splice the serial section image obtained by the image obtaining unit so as to obtain an integral digital image of a test sample.
Description
Technical field
The utility model relates to the robotization imaging system of a kind of large scale biological tissue serial section, particularly software control system to be realized the robotization imaging of magnanimity large scale biological tissue serial section and the splicing of two-dimensional digital image.
Background technology
The imaging of human pathology's section or biological tissue section is the necessary means of obtaining information.Particularly for the section of hospital pathology department magnanimity, or a large amount of serial section of producing in order to study mechanics of biological tissue of laboratory, its workload with artificial imaging is huge, time-consuming, effort.In addition, the visual field of conventional optical microscope is limited, is far smaller than the size of pathological section and biological tissue section, and the digital image information that provides section complete is provided.
In addition, for business-like scanning imagery equipment, general integrated microscope and other peripherals, the systematic comparison complexity, cost is also higher.
Thus, the robotization imaging system of a kind of large scale of magnanimity cheaply of industry needs biological tissue serial section.
The utility model content
In order to reach above-mentioned purpose, the utility model aims to provide the robotization imaging system of a kind of large scale of magnanimity cheaply biological tissue serial section.
For this reason, the utility model provides the imaging system of a kind of large scale biological tissue serial section, comprising: image acquisition unit, and it obtains the digitized image of described serial section; Concatenation unit, thus it carries out to described image the complete image that the two dimension splicing forms the large scale section; And control module, it is controlled described image acquisition unit and automatically carries out imaging and control described concatenation unit automatically being spliced.Described control module is controlled the image acquisition unit Automatic continuous and is obtained the image of described section, and controls and get the serial section image splicing of described concatenation unit to being obtained by described image acquisition unit, thereby obtains the complete digitized image of sample.
In some embodiment, described image-generating unit comprises: optical module, and it is in order to collect the optical signalling of transmission from described serial section; Image-forming assembly, it is in order to be converted to data image signal by described optical signalling; And light source assembly, it provides illumination for described optical module.
In some embodiment, described optical module comprises: objective lens, and it comprises the object lens of different amplification and numerical aperture, described control module is selected the object lens of different numerical apertures according to the requirement of different resolution; And convertible optical filtering film magazine, it comprises a plurality of filter set of selecting particular range of wavelengths, described control module switches different filter set according to predefined imaging requirements, thereby makes the described system can be to the biologic slice imaging of samples of different colours mark.
In some embodiment, described light source is light emitting diode, laser instrument, or mercury lamp light source.
In some embodiment, described image-forming assembly is camera.
In some embodiment, described camera is beam coupling device (CCD), or complementary matal-oxide semiconductor (CMOS) camera.
In some embodiment, described imaging system also comprises and prevents focal length drift unit, in described imaging process, it utilizes the light of specific wavelength to lock the distance of described objective lens and described section at the echo of the boundary reflection of described objective lens and described section, thereby the defocusing phenomenon that cause the shift position that prevents from cutting into slices, guarantee picture quality.
In some embodiment, described control module, after adjusting the position of described objective table, then carries out the switching of described filter set, thereby realizes several figure of same position automatic shooting different colours.
In some embodiment, described concatenation unit comprises: for the section digital picture obtained, do background, after the processing of the necessity such as normalization, the size of the overlapping region between the continuous two width images of setting during according to shooting and the degree of correlation of overlapping region image information realize the splicing of multiple images, finally obtain the whole number image of large scale section.
The continuous large scale of native system biological tissue serial section robotization imaging system, by to motorized subject table, objective lens, filter set, lighting source, take the automatic control of camera, can realize the robotization imaging to magnanimity large scale biological tissue serial section, can provide the light field imaging also can provide fluorescence imaging, simplified the complexity of prior art and reduced cost.Simultaneously, at same position, by the conversion of optical filter, can photograph the image of different wavelength range, especially at the biological sample of observing multiple label mark, very important using value be arranged.
By reference to the accompanying drawings, according to hereinafter illustrate that by example the description of the utility model purport can know other side of the present utility model and advantage.
The accompanying drawing explanation
By reference to the accompanying drawings, by hereinafter describing in detail, can more clearly understand above-mentioned and other feature and advantage of the present utility model, wherein:
Fig. 1 is the structural representation according to a kind of large scale of the present utility model biological tissue serial section imaging system; And
Fig. 2 is the structural representation according to the example of a kind of large scale of the present utility model biological tissue serial section imaging system.
Embodiment
Referring to the accompanying drawing of the utility model embodiment, hereinafter the utility model will be described in more detail.Yet the utility model can be with many multi-form realizations, and should not be construed as the restriction of the embodiment be subject in this proposition.On the contrary, it is abundant and complete open in order to reach proposing these embodiment, and makes those skilled in the art understand scope of the present utility model fully.In these accompanying drawings, for clarity sake, may carry out amplifying or distortion to size and the relative size in layer and zone.
The part that should be understood that description of the present utility model/be illustrated as individual unit can be present in two or more physically independent but cooperation realizes the entity of the function of describe/diagram.In addition, describe/be illustrated as two or more and physically independently partly can be integrated into an independent physically entity to carry out described/illustrated function.
Refer now to Fig. 1 and describe the imaging system according to the large scale biological tissue serial section of the utility model embodiment in detail.As shown in Figure 1, according to the imaging system of the large scale biological tissue serial section of the utility model embodiment, comprise image acquisition unit, it obtains the digitized image of described serial section; Concatenation unit, thus it carries out to described image the complete image that the two dimension splicing forms the large scale section; And control module, it is controlled described image acquisition unit and automatically carries out imaging and control described concatenation unit automatically being spliced.Described control module is controlled the image acquisition unit Automatic continuous and is obtained the image of described section, and controls and get described concatenation unit the serial section image obtained by described image acquisition unit is spliced, thereby obtains the complete digitized image of sample.
In the present embodiment, described image-generating unit comprises optical module, and it is in order to collect the optical signalling of transmission from described serial section; Image-forming assembly, it is in order to be converted to data image signal by described optical signalling; And light source assembly, it provides illumination for described optical module.
Particularly, described optical module comprises objective lens, and it comprises the object lens of different amplification and numerical aperture, and described control module is selected the object lens of different numerical apertures according to the requirement of different resolution; And convertible optical filtering film magazine.Described convertible optical filtering film magazine comprises a plurality of filter set of selecting particular range of wavelengths, described control module switches different filter set according to predefined imaging requirements, thereby makes the described system can be to the biologic slice imaging of samples of different colours mark.
In preferred embodiment, described light source is light emitting diode, laser instrument, or mercury lamp light source.
In addition, in the present embodiment, described image-forming assembly is camera.Particularly, described camera is beam coupling device (CCD), or complementary matal-oxide semiconductor (CMOS) camera.
In preferred embodiment, described imaging system also comprises and prevents focal length drift unit, in described imaging process, it utilizes the light of specific wavelength to lock the distance of described objective lens and described section at the echo of the boundary reflection of described objective lens and described section, thereby the defocusing phenomenon that cause the shift position that prevents from cutting into slices, guarantee picture quality.
In addition, described control module, after adjusting the position of described objective table, then carries out the switching of described filter set, thereby realizes several figure of same position automatic shooting different colours.
In preferred embodiment, described concatenation unit also comprises: for the section digital picture obtained, do background, after the processing of the necessity such as normalization, the size of the overlapping region between the continuous two width images of setting during according to shooting and the degree of correlation of overlapping region image information realize the splicing of multiple images, finally obtain the whole number image of large scale section.
Refer now to Fig. 2 and describe the example of realizing occurring according to system of the present utility model system in detail.
The structure intention of the example that Fig. 2 is a kind of large scale of native system biological tissue serial section automatic imaging system.As shown in Figure 2, native system is a kind of robotization imaging system, comprising: optical imagery unit 10, prevent focus drifting unit 11, computer control system 12 and camera 13.
Motorized subject table 100 is for placing sample, in practical operation, select interested zone in sample, generally pass through two of this zone of selection to angular coordinate, thereby determine the rectangular area of section, then by computing machine 12 software control motorized subject table 100 and CCD13, realize that sample moves a position, get figure, then move and get the circulation of figure, until take complete selected zone.Consider the processing such as splicing of later image, in clapping the figure process, can automatically calculate and set out the size of overlapping region between adjacent two width figure.
In imaging process, prevent that illumination that focus drifting system 11 sends specific wavelength is at sample surfaces, and collect the light signal be reflected back and the height of object lens is done to real-time adjustment, the defocusing phenomenon produced to prevent sample from moving on a large scale.
If sample is used common dye dyeing, system adopts bright field illumination mode (LED); If the antibody labeling of fluorescin or fluorescence molecule mark for sample, adopt LED, laser or mercury lamp illumination.
The continuous large scale of native system biological tissue serial section robotization imaging system, by to motorized subject table, objective lens, filter set, lighting source, take the automatic control of camera, can realize the robotization imaging to magnanimity large scale biological tissue serial section, can provide the light field imaging also can provide fluorescence imaging, simplified the complexity of prior art and reduced cost.Simultaneously, at same position, by the conversion of optical filter, can photograph the image of different wavelength range, especially at the biological sample of observing multiple label mark, very important using value be arranged.
Because should be understood that the utility model, those skilled in the art can realize not breaking away from spirit or scope of the present utility model with many other concrete forms.Although already described embodiment of the present utility model, the utility model should be understood and these embodiment should be restricted to, make and change and revise within the utility model spirit and scope that those skilled in the art can define as appended claims.
Claims (9)
1. the robotization imaging system of a large scale biological tissue serial section comprises:
Image acquisition unit, it obtains the digitized image of described serial section;
Concatenation unit, thus it carries out to described image the complete image that the two dimension splicing forms the large scale section; And
Control module, it is controlled described image acquisition unit and automatically carries out imaging and control described concatenation unit automatically being spliced,
Wherein said control module is controlled the image acquisition unit Automatic continuous and is obtained the image of described section, and controls and get described concatenation unit the serial section image obtained by described image acquisition unit is spliced, thereby obtains the complete digitized image of sample.
2. according to the described robotization imaging system of right 1, it is characterized in that, described image-generating unit comprises:
Optical module, it is in order to collect the optical signalling of transmission from described serial section;
Image-forming assembly, it is in order to be converted to data image signal by described optical signalling; And
Light source assembly, it provides illumination for described optical module.
3. according to the described robotization imaging system of right 2, it is characterized in that, described optical module comprises:
Objective lens, it comprises the object lens of different amplification and numerical aperture, described control module is selected the object lens of different numerical apertures according to the requirement of different resolution; And
Convertible optical filtering film magazine, it comprises a plurality of filter set of selecting particular range of wavelengths, described control module switches different filter set according to predefined imaging requirements, thereby makes the described system can be to the biologic slice imaging of samples of different colours mark.
4. according to the described robotization imaging system of right 2, it is characterized in that, described light source is light emitting diode, laser instrument, or mercury lamp light source.
5. according to the described robotization imaging system of right 2, it is characterized in that, described image-forming assembly is camera.
6. according to the described robotization imaging system of right 5, it is characterized in that, described camera is the beam coupling device, or complementary matal-oxide semiconductor camera.
7. according to the described robotization imaging system of right 1, it is characterized in that, described imaging system also comprises and prevents focal length drift unit, in described imaging process, it utilizes the light of specific wavelength to lock the distance of described objective lens and described section at the echo of the boundary reflection of described objective lens and described section, thereby the defocusing phenomenon that cause the shift position that prevents from cutting into slices, guarantee picture quality.
8. according to the described robotization imaging system of right 1, it is characterized in that, described control module, after adjusting the position of described objective table, then carries out the switching of described filter set, thereby realizes several figure of same position automatic shooting different colours.
9. according to the described robotization imaging system of right 1, it is characterized in that, its described concatenation unit comprises: after doing background and normalized for the section digital picture obtained, between the continuous two width images of setting during according to shooting, the degree of correlation of the size of overlapping region and overlapping region image information realizes the splicing of multiple images, finally obtains the whole number image of large scale section.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201320129316.1U CN203385649U (en) | 2013-03-20 | 2013-03-20 | Automatic imaging system for large-scale biological tissue serial section |
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| CN201320129316.1U CN203385649U (en) | 2013-03-20 | 2013-03-20 | Automatic imaging system for large-scale biological tissue serial section |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103175780A (en) * | 2013-03-20 | 2013-06-26 | 上海交通大学 | Automated imaging system of large-size serial section of biological tissue |
| CN105960586A (en) * | 2014-01-30 | 2016-09-21 | Bd科斯特公司 | A system and method for image acquisition using supervised high quality imaging |
-
2013
- 2013-03-20 CN CN201320129316.1U patent/CN203385649U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103175780A (en) * | 2013-03-20 | 2013-06-26 | 上海交通大学 | Automated imaging system of large-size serial section of biological tissue |
| CN105960586A (en) * | 2014-01-30 | 2016-09-21 | Bd科斯特公司 | A system and method for image acquisition using supervised high quality imaging |
| US10200625B2 (en) | 2014-01-30 | 2019-02-05 | Bd Kiestra B.V. | System and method for image acquisition using supervised high quality imaging |
| CN105960586B (en) * | 2014-01-30 | 2020-03-03 | Bd科斯特公司 | System and method for image acquisition using supervised high quality imaging |
| US10841503B2 (en) | 2014-01-30 | 2020-11-17 | Bd Kiestra B.V. | System and method for image acquisition using supervised high quality imaging |
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