CN203191381U - Test standard plate for raster type fluorescent Elisa analyzer - Google Patents
Test standard plate for raster type fluorescent Elisa analyzer Download PDFInfo
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- CN203191381U CN203191381U CN 201320170486 CN201320170486U CN203191381U CN 203191381 U CN203191381 U CN 203191381U CN 201320170486 CN201320170486 CN 201320170486 CN 201320170486 U CN201320170486 U CN 201320170486U CN 203191381 U CN203191381 U CN 203191381U
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Abstract
The utility model discloses a test standard plate for a raster type fluorescent Elisa analyzer. The standard plate comprises a standard substrate; Elisa holes having the same specification and arranged regularly are installed in the standard substrate; each Elisa hole is a cylindrical structure with a closed bottom; a matrix is fixed at the internal bottom of each Elisa hole; a solid fluorescent material is coated on the upper surface of the matrix; the matrix and the corresponding solid fluorescent material form a fluorescent film; and the representations of the transmission wavelength and excitation wavelength of the fluorescent film are used as standard values of the standard plate. The test standard plate for the raster type fluorescent Elisa analyzer is simple in structure, unified in standard, easy to operate and maintain, and low in price.
Description
Technical field
The utility model relates to a kind of analyser testing apparatus, relates in particular to a kind of raster pattern fluorescence enzyme micro-plate reader testing standard plate.
Background technology
Fluorescence is a kind of luminescence phenomenon that the material stimulated radiation produces.When material molecule is subjected to the light source irradiation of specific wavelength, the molecule absorption photon also makes the electronics induced transition that is in ground state to excited state, be in the electronics instability of excited state, return energy unnecessary in the process of ground state in transition and discharge with the form of photon, thereby produce luminescence phenomenon.The light that sends by this kind mode is called fluorescence.
The fluorescence enzyme micro-plate reader is to utilize fluorescence phenomenon to carry out the instrument of material qualitative and quantitative analysis instrument, and it has little, the highly sensitive characteristics of background interference.The fluorescence enzyme micro-plate reader combines high flux and the highly sensitive advantage of fluorescence signal of immunoreactive high specific, enzyme micro-plate reader, has obtained in fields such as food security, clinical examinations in recent years using widely.The common plate of fluorescence enzyme micro-plate reader can carry out the detection of 96 or 384 samples, and can use the third party to detect reagent.Fluorescence enzyme micro-plate reader testing process and principle are generally as follows: at first wrap antibody on the quilt in ELISA Plate, then sample to be detected is joined in the enzyme mark hole, testing compound in the sample is caught with regard to the specific antibody that is coated on the enzyme mark hole, detect antibody through adding again after the washing, detect the object of antibody on being combined in capture antibody and be combined again, form the structure of " sandwich hamburger formula ".Then anti-through the further enzyme di-that adds in washing back, the anti-detection antibody in " sandwich hamburger compound " of enzyme di-is combined.After washing, further add fluorogenic substrate, reacting under the effect of this substrate enzyme on the enzyme di-is anti-generates the substrate with intense fluorescence signal.The amount positive correlation of fluorescence intensity and product, the content positive correlation of target compound in the amount of product and the sample to be detected.Therefore, can judge the content of target compound in the testing sample according to fluorescence signal intensity in the enzyme mark hole, reaction back.
Whether accurate the metering performance of and the instrument of fluorescence enzyme micro-plate reader testing result be closely related, these metering performances comprise indexs such as emission wavelength accuracy, excitation wavelength accuracy, sensitivity, repeatability, and the affirmation of these metering performance indexs and calibration must realize by measurement standard.The fluorescence enzyme micro-plate reader is broadly divided into optical filtering chip or two kinds of structures of raster pattern from the optical texture principle, and the wavelength accuracy of optical filtering chip luciferase mark analyser can remove optical filter and detect calibration through the qualified ultraviolet spectrophotometer of measurement verification.But the fluorescence enzyme micro-plate reader measurement standard of raster pattern comparatively is short of, structure is various, also do not formulate corresponding instrument vertification regulation or calibrating standard, can't carry out calibrating or the calibration of instrument, the measurement standard of development raster pattern fluorescence enzyme micro-plate reader becomes the task of top priority.
The utility model content
The purpose of this utility model is to provide a kind of simple in structure, raster pattern fluorescence enzyme micro-plate reader testing standard plate that standard is unified.
For achieving the above object, the utility model provides a kind of raster pattern fluorescence enzyme micro-plate reader testing standard plate, comprise standard substrate, several same specifications and regularly arranged enzyme mark hole are installed in the described standard substrate, the tubular construction of each described enzyme mark Kong Weiyi base seal, the inner bottom part in each described enzyme mark hole fixedly has matrix, the upper surface of described matrix is coated with the solid fluorescence material, described matrix and the solid fluorescence material corresponding with it constitute fluorescence membrane, and the emission wavelength of described fluorescence membrane and the sign of excitation wavelength are as the standard value of this on-gauge plate.
Raster pattern fluorescence enzyme micro-plate reader testing standard plate of the present utility model, described matrix is papery or high molecular polymer material.
Raster pattern fluorescence enzyme micro-plate reader testing standard plate of the present utility model, described solid fluorescence material is fluorescein, cy3, cy5, Cy7, PE-Cy5, PE-Cy7, Alexa Fluor350, fluorescein isothiocynate, Alexa Fluor488, Cy3, Alexa Fluor555, rhodamine, PE, PE-Cy3, APC or Alexa Fluor647.
Raster pattern fluorescence enzyme micro-plate reader testing standard plate of the present utility model comprises standard substrate, several same specifications and regularly arranged enzyme mark hole are installed in the standard substrate, the tubular construction of each enzyme mark Kong Weiyi base seal, the inner bottom part in each enzyme mark hole fixedly has matrix, the upper surface of matrix is coated with the solid fluorescence material, matrix and the solid fluorescence material corresponding with it constitute fluorescence membrane, and the emission wavelength of fluorescence membrane and the sign of excitation wavelength are as the standard value of this on-gauge plate.A kind of simple in structure, standard is unified, easy operating is safeguarded, low-cost fluorescence enzyme micro-plate reader metrology and measurement on-gauge plate thereby provide.
Description of drawings
Fig. 1 is the spatial structure cut-open view of raster pattern fluorescence enzyme micro-plate reader testing standard plate of the present utility model;
Fig. 2 is the A place enlarged drawing of Fig. 1.
Embodiment
Below in conjunction with accompanying drawing embodiment of the present utility model is described in detail:
With reference to illustrated in figures 1 and 2, the raster pattern fluorescence enzyme micro-plate reader testing standard plate of present embodiment comprises standard substrate 1,96 same specifications and regularly arranged enzyme mark hole 2 are installed in the standard substrate 1, each enzyme mark hole 2 is the columnar structured of a base seal, the inner bottom part in each enzyme mark hole 2 is adhesively fixed with the matrix 3 that its material is papery or high molecular polymer, the upper surface of matrix 3 is coated with for example fluorescein of solid fluorescence material 4(, cy3, cy5, Cy7, PE-Cy5, PE-Cy7, Alexa Fluor350, fluorescein isothiocynate, Alexa Fluor488, Cy3, Alexa Fluor555, rhodamine, PE, PE-Cy3, APC, or Alexa Fluor647 etc.), matrix 3 and the solid fluorescence material 4 corresponding with it constitute fluorescence membrane, and the emission wavelength of fluorescence membrane and the sign of excitation wavelength are as the standard value of this on-gauge plate.
In the present embodiment, above-mentioned raster pattern fluorescence enzyme micro-plate reader testing standard plate can adopt following processes to obtain, and specifically may further comprise the steps:
Step 4, the matrix 3 that is combined with solid fluorescence material 4 on it is cut into and enzyme mark hole 2 internal diameters circular fluorescent film of the same size, and adopts bonding agent the circular fluorescent film to be bonded on the inner bottom part in enzyme mark hole 2;
Step 5, the enzyme mark hole 2 that each its inner bottom part is provided with fluorescence membrane fixedly are assemblied in the standard substrate 1.
When the raster pattern fluorescence enzyme micro-plate reader testing standard plate of present embodiment uses:
When being used for excitation wavelength error of indication metrology and measurement, in raster pattern fluorescence enzyme micro-plate reader, fix a certain selected emission wavelength, scan excitation wavelength then, obtain the wavelength X i at each peak in the excitation spectrum, and compare with standard value λ s, obtain the error of indication Δ λ i of one group of excitation wavelength, get wherein the maximum as the error of indication report value of excitation wavelength.When being used for emission wavelength error of indication metrology and measurement, in raster pattern fluorescence enzyme micro-plate reader, fix a certain selected excitation wavelength, scan emission wavelength then, obtain the wavelength X i at each peak in the emission spectrum, and compare with standard value λ s, obtain the error of indication Δ λ i of one group of emission wavelength, get wherein the maximum as the error of indication report value of excitation wavelength.
Above embodiment is described preferred implementation of the present utility model; be not that scope of the present utility model is limited; under the prerequisite that does not break away from the utility model design spirit; various distortion and improvement that the common engineering technical personnel in this area make the technical solution of the utility model all should fall in the definite protection domain of claims of the present utility model.
Claims (3)
1. raster pattern fluorescence enzyme micro-plate reader testing standard plate, it is characterized in that, comprise standard substrate (1), several same specifications and regularly arranged enzyme mark hole (2) are installed in the described standard substrate (1), each described enzyme mark hole (2) is the tubular construction of a base seal, the inner bottom part in each described enzyme mark hole (2) fixedly has matrix (3), the upper surface of described matrix (3) is coated with solid fluorescence material (4), described matrix (3) and the solid fluorescence material (4) corresponding with it constitute fluorescence membrane, and the emission wavelength of described fluorescence membrane and the sign of excitation wavelength are as the standard value of this on-gauge plate.
2. raster pattern fluorescence enzyme micro-plate reader testing standard plate according to claim 1 is characterized in that described matrix (3) is papery or high molecular polymer material.
3. raster pattern fluorescence enzyme micro-plate reader testing standard plate according to claim 2, it is characterized in that described solid fluorescence material (4) is fluorescein, cy3, cy5, Cy7, PE-Cy5, PE-Cy7, Alexa Fluor350, fluorescein isothiocynate, Alexa Fluor488, Cy3, Alexa Fluor555, rhodamine, PE, PE-Cy3, APC or Alexa Fluor647.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320170486 CN203191381U (en) | 2013-04-08 | 2013-04-08 | Test standard plate for raster type fluorescent Elisa analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320170486 CN203191381U (en) | 2013-04-08 | 2013-04-08 | Test standard plate for raster type fluorescent Elisa analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203191381U true CN203191381U (en) | 2013-09-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320170486 Withdrawn - After Issue CN203191381U (en) | 2013-04-08 | 2013-04-08 | Test standard plate for raster type fluorescent Elisa analyzer |
Country Status (1)
| Country | Link |
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| CN (1) | CN203191381U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103217526A (en) * | 2013-04-08 | 2013-07-24 | 中国计量科学研究院 | Grating type luciferase label analysis meter testing standard plate and processing technology thereof |
-
2013
- 2013-04-08 CN CN 201320170486 patent/CN203191381U/en not_active Withdrawn - After Issue
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103217526A (en) * | 2013-04-08 | 2013-07-24 | 中国计量科学研究院 | Grating type luciferase label analysis meter testing standard plate and processing technology thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20130911 Effective date of abandoning: 20150121 |
|
| RGAV | Abandon patent right to avoid regrant |